LOCUS       CMV-mCyRFP2_-PTE        6615 bp DNA     circular SYN 24-SEP-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 24, 2024 from SnapGene 7.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6615
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     complement(591..612)
                     /label=V REV BPHP SR
                     /note="color: black; sequence: GGTGGCGACCGGTAGCGCTAGC; 
                     added: 2022-02-27"
     primer_bind     591..612
                     /label=I1 For SR BPHP
                     /note="color: black; sequence: GCTAGCGCTACCGGTCGCCACC; 
                     added: 2022-02-27"
     primer_bind     613..634
                     /label=I_Mar_For
                     /note="color: black; sequence: 
                     ttacaaaagtccctacccctatggtgagcaagggcgaggagc; added: 
                     2022-02-27"
     misc_feature    616..1314
                     /label=mCyRFP2
                     /note="color: #a6acb3"
     primer_bind     complement(1295..1335)
                     /label=I1 Cy Rev
                     /note="color: black; sequence: 
                     AGCTCGAGATCTGAGTCCGGActtgtacagctcgtccatgc; added: 
                     2022-02-27"
     primer_bind     complement(1298..1315)
                     /label=I2 REV Mar
                     /note="color: black; sequence: 
                     GCTGATTATGATCAGTTATCTttacttgtacagctcgtcca; added: 
                     2022-02-27"
     primer_bind     1315..1335
                     /label=Primer 89 SGLRSA For
                     /note="color: black; sequence: TCCGGACTCAGATCTCGAGCT; 
                     added: 2022-02-27"
     misc_feature    1336..2544
                     /label=PTEN
                     /note="color: #a6acb3"
     misc_feature    1375..1377
                     /label=R14G
                     /note="color: #a6acb3"
     primer_bind     complement(1389..1409)
                     /label=PTEN N rev
                     /note="color: black; sequence: aagtctaagtcgaatccatcc; 
                     added: 2022-01-02"
     primer_bind     complement(2449..2481)
                     /label=PTEN_4A_R
                     /note="color: black; sequence: 
                     tgcatctgcatatctataatgatcaggttcatt; added: 2021-10-07"
     misc_feature    2473..2490
                     /label=fragment 4A
                     /note="color: #a6acb3"
     misc_feature    2473..2490
                     /label=380-SDTTDS-385 TO 380- ADAADA-385
                     /note="color: #a6acb3"
     primer_bind     2482..2511
                     /label=PTEN_4A_F
                     /note="color: black; sequence: 
                     gcagatgcagatccagagaatgaacctttt; added: 2021-10-07"
     primer_bind     2491..2504
                     /label=PTEN_C_For
                     /note="color: black; sequence: gactctgatccagagaatga; added:
                     2021-07-15"
     primer_bind     2534..2575
                     /label=I_Mar_For
                     /note="color: black; sequence: 
                     ttacaaaagtccctacccctatggtgagcaagggcgaggagc; added: 
                     2022-02-27"
     primer_bind     complement(2534..2553)
                     /label=VRev 2
                     /note="color: black; sequence: aggggtagggacttttgtaa; added:
                     2022-02-27"
     misc_feature    2545..2553
                     /label=linker PTP
                     /note="color: #a6acb3"
     CDS             2554..3288
                     /codon_start=1
                     /product="bright, rapidly maturing, monomeric far-red 
                     fluorescent protein (Bajar et al., 2016)"
                     /label=mMaroon1
                     /note="color: #a0003c"
                     /translation="MVSKGEELIKENMHTKLYLTGTVNNHYFECTAEGEGKPYEGTQTN
                     RIKVVRGGPLPFAFDILAPCFMYGSKTFINHPPDIPDYFKQSFPEGFTWERTTVYEDGG
                     TLTATQDTSLQDGCLIYNVQVRGENFPSNGPVMQKKTLGWEASTETLYPADGSLEGRLD
                     WALKLVGGGHLHCRLETTYRSKKPAKNLKMPGVYFIDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     primer_bind     complement(3266..3286)
                     /label=I1 Cy Rev
                     /note="color: black; sequence: 
                     AGCTCGAGATCTGAGTCCGGActtgtacagctcgtccatgc; added: 
                     2022-02-27"
     primer_bind     complement(3269..3309)
                     /label=I2 REV Mar
                     /note="color: black; sequence: 
                     GCTGATTATGATCAGTTATCTttacttgtacagctcgtcca; added: 
                     2022-02-27"
     primer_bind     3289..3309
                     /label=primer 42'
                     /note="color: black; sequence: AGATAACTGATCATAATCAGC; 
                     added: 2021-11-16"
     polyA_signal    3403..3524
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      complement(3531..3986)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        4013..4117
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     promoter        4119..4476
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      4327..4462
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     CDS             4511..5305
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin)"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    5537..5584
                     /label=HSV TK poly(A) signal
                     /note="herpesvirus thymidine kinase polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      5913..6501
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgatca aggaggaaat gaccagcaag
      661 ctgtacctgg aaggcagcgt gaacggccac tacttcaagt gcacccacga aggggagggc
      721 aagccctacg agggcaagca gaccgccagg atcaaggtgg tggagggagg ccccctgccg
      781 ttcgcattcg acatcctggc cacccacttt atgtacggga gcaaggtgtt catcaagtac
      841 cccgccgaca tccccgatta ttttaagcag tccttccctg agggcttcac atgggagaga
      901 gtcatggtgt tcgaagacgg gggcgtgctg accactaccc aggacaccag cctccaggac
      961 ggcgtgctca tctacaacgt caagaccaga ggggtgaact tcccagccaa cggccccgtg
     1021 atgcagaaga aaacactggg ctgggagccc agcaccgaga ccatgtaccc cgccgacggc
     1081 ggcctggaag gcagatgcga caagaagctg aagctcgtgg gcgggggcca cctgcacgtc
     1141 aacttcaaga ccacatacaa gtccaagaaa cccgtgaaga tgcccggcgt ccactacgtg
     1201 gacaggagac tggaaagaat caaggaggcc gacaacgaga cctacgtcga gcagtacgag
     1261 cacgctgtgg ccagatactc caacctgggc ggaggcatgg acgagctgta caagtccgga
     1321 ctcagatctc gagctatgac agccatcatc aaagagatcg ttagcagaaa caaagggaga
     1381 tatcaagagg atggattcga cttagacttg acctatattt atccaaatat tattgctatg
     1441 ggatttcctg cagaaagact tgaaggtgta tacaggaaca atattgatga tgtagtaagg
     1501 tttttggatt caaagcataa aaaccattac aagatataca atctatgtgc tgagagacat
     1561 tatgacaccg ccaaatttaa ctgcagagtt gcacagtatc cttttgaaga ccataaccca
     1621 ccacagctag aacttatcaa acccttttgt gaagatcttg accaatggct aagtgaagac
     1681 gacaatcatg ttgcagcaat tcactgtaaa gctgggaaag gacggactgg tgtaatgatt
     1741 tgtgcatatt tattgcatcg gggcaagttt ttaaaggcac aagaggccct ggatttttat
     1801 ggggaagtaa ggaccagaga taaaaaggga gtaactattc ccagtcagag gcgctatgta
     1861 tattattata gctacctgtt aaagaatcac ctggattaca gaccagtggc actgttgttt
     1921 cacaagatga tgtttgaaac tattccaatg ttcagtggcg gaacttgcaa tccccagttt
     1981 gtggtctgcc agctaaaggt gaagatctac tcctccaact caggacccac gcggcgggag
     2041 gacaagctca tgtactttga gttccctcag ccattgcctg tgtgtggtga catcaaagta
     2101 gagttcttcc acaaacagaa caagatgctc aaaaaggaca aaatgtttca cttttgggta
     2161 aatacgttct tcataccagg accagaggaa acctcagaaa aagtggaaaa tggaagtctt
     2221 tgtgatcagg aaatcgatag catttgtagt atagagcgtg cggataatga caaggagtat
     2281 cttgtgctca ccctgacaaa aaatgatctt gacaaagcaa acaaagacaa ggccaaccga
     2341 tacttctctc caaattttaa ggtgaaatta tactttacaa aaacagtaga ggaaccatca
     2401 aatccagagg ctagcagttc aacttctgtg actccagacg ttagtgacaa tgaacctgat
     2461 cattatagat atgcagatgc agcagatgca gatccagaga atgaaccttt tgatgaagat
     2521 cagcattcac aaattacaaa agtccctacc cctatggtga gcaagggcga ggagctgatc
     2581 aaggagaaca tgcacaccaa gctgtacctg acaggcaccg tgaacaacca ctacttcgaa
     2641 tgcaccgccg aaggggaggg caagccctac gagggcaccc agaccaacag gatcaaggtt
     2701 gtgcgcggag gccccctgcc gttcgcattc gacatcctgg ccccctgctt tatgtacggg
     2761 agcaagacct tcattaacca tccacctgac attcccgatt attttaagca gtccttccct
     2821 gagggcttca catgggagag aaccaccgtt tacgaagacg ggggcacact cactgctaca
     2881 caggacacca gcctccagga cggctgcctg atctacaacg tccaagtcag aggggagaac
     2941 ttcccatcca acggccctgt gatgcagaag aaaacactcg gctgggaggc cagtaccgag
     3001 accctgtacc ccgctgacgg cagcctggaa ggcagactgg actgggccct gaagctcgtg
     3061 ggcgggggcc acctgcactg cagactggaa accacataca gatccaagaa acccgcaaag
     3121 aacctcaaga tgcccggcgt ctattttatt gacaggagac tggaaagaat caaggaggcc
     3181 gacaatgaga cctacgtcga gcagcacgag gtggctgtgg ccagatactg cgacctccct
     3241 agcaaactgg ggcacaaact taatggcatg gacgagctgt acaagtaaag ataactgatc
     3301 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
     3361 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
     3421 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     3481 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaacgcgt aaattgtaag
     3541 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     3601 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     3661 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     3721 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
     3781 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     3841 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     3901 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     3961 gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac
     4021 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     4081 ctgataaatg cttcaataat attgaaaaag gaagagtcct gaggcggaaa gaaccagctg
     4141 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     4201 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     4261 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     4321 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     4381 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     4441 tgaggaggct tttttggagg cctaggcttt tgcaaagatc gatcaagaga caggatgagg
     4501 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     4561 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     4621 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     4681 gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     4741 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     4801 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     4861 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     4921 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     4981 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag
     5041 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     5101 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     5161 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     5221 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     5281 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
     5341 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     5401 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     5461 gagttcttcg cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa
     5521 ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt
     5581 gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc
     5641 cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt
     5701 gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc ctcaggttac
     5761 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     5821 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     5881 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     5941 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     6001 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     6061 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     6121 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     6181 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     6241 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     6301 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     6361 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     6421 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     6481 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     6541 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     6601 attaccgcca tgcat
//
