LOCUS       pk335-CARGO-3mer-35kb-DSF 6597 bp    DNA     circular SYN 05-SEP-2024
DEFINITION  pk335-CARGO-3mer-35kb-DSF.
ACCESSION   pk335-CARGO-3mer-35kb-DSF
VERSION     pk335-CARGO-3mer-35kb-DSF
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     enhancer        2162..2447
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     intron          2732..2959
                     /note="pLannotate"
                     /label="hybrid intron"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="intron"
     repeat_region   3992..4132
                     /note="pLannotate"
                     /label="AAV2 ITR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="repeat_region"
     polyA_signal    3709..3820
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     CDS             5280..6083
                     /note="pLannotate"
                     /label="NeoR/KanR"
                     /database="snapgene"
                     /identity="99.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     protein_bind    3920..3989
                     /note="pLannotate"
                     /label="attB"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             2977..3678
                     /note="pLannotate"
                     /label="TagBFP"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      1..589
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        5213..5260
                     /note="pLannotate"
                     /label="EM7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        4705..5191
                     /note="pLannotate"
                     /label="PGK promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="97.4"
                     /fragment="False"
                     /other="promoter"
     rep_origin      4207..4662
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="98.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        2449..2731
                     /note="pLannotate"
                     /label="chicken β-actin promoter"
                     /database="snapgene"
                     /identity="98.2"
                     /match_length="101.8"
                     /fragment="False"
                     /other="promoter"
     promoter        768..999
                     /note="pLannotate"
                     /label="U6 promoter"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="96.3"
                     /fragment="False"
                     /other="promoter"
     promoter        1146..1377
                     /note="pLannotate"
                     /label="U6 promoter"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="96.3"
                     /fragment="False"
                     /other="promoter"
     promoter        1524..1755
                     /note="pLannotate"
                     /label="U6 promoter"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="96.3"
                     /fragment="False"
                     /other="promoter"
     promoter        1902..2133
                     /note="pLannotate"
                     /label="U6 promoter"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="96.3"
                     /fragment="False"
                     /other="promoter"
     CDS             6132..6426
                     /note="pLannotate"
                     /label="bla(M) (fragment)"
                     /database="snapgene"
                     /identity="98.6"
                     /match_length="37.1"
                     /fragment="True"
                     /other="CDS"
     misc_RNA        669..736
                     /note="pLannotate"
                     /label="tracrRNA (fragment)"
                     /database="snapgene"
                     /identity="98.5"
                     /match_length="86.1"
                     /fragment="True"
                     /other="misc_RNA"
     misc_RNA        1047..1114
                     /note="pLannotate"
                     /label="tracrRNA (fragment)"
                     /database="snapgene"
                     /identity="98.5"
                     /match_length="86.1"
                     /fragment="True"
                     /other="misc_RNA"
     misc_RNA        1425..1492
                     /note="pLannotate"
                     /label="tracrRNA (fragment)"
                     /database="snapgene"
                     /identity="98.5"
                     /match_length="86.1"
                     /fragment="True"
                     /other="misc_RNA"
     misc_RNA        1803..1870
                     /note="pLannotate"
                     /label="tracrRNA (fragment)"
                     /database="snapgene"
                     /identity="98.5"
                     /match_length="86.1"
                     /fragment="True"
                     /other="misc_RNA"
     misc_feature    complement(4137..4188)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="36.9"
                     /fragment="True"
                     /other="misc_feature"
     misc_feature    complement(4663..4704)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="29.8"
                     /fragment="True"
                     /other="misc_feature"
     polyA_signal    6087..6123
                     /note="pLannotate"
                     /label="PGK poly(A) signal (fragment)"
                     /database="snapgene"
                     /identity="97.3"
                     /match_length="8.0"
                     /fragment="True"
                     /other="polyA_signal"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt gtttaagagc
      661 tatgctggaa acagcatagc aagtttaaat aaggctagtc cgttatcaac ttgaaaaagt
      721 ggcaccgagt cggtgctttt tttctcgagt actaggatcc attaggcttt cccatgattc
      781 cttcatattt gcatatacga tacaaggctg ttagagagat aattggaatt aatttgactg
      841 taaacacaaa gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt
      901 ttgcagtttt aaaattatgt tttaaaatgg actatcatat gcttaccgta acttgaaagt
      961 atttcgattt cttggcttta tatatcttgt ggaaaggacg aaacaccgct gcagatacct
     1021 gcagagttgt ttaagagcta tgctggaaac agcatagcaa gtttaaataa ggctagtccg
     1081 ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt tctcgagtac taggatccat
     1141 taggctttcc catgattcct tcatatttgc atatacgata caaggctgtt agagagataa
     1201 ttggaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt
     1261 aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc
     1321 ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa
     1381 acaccgctta atcatgtctg tttaccgttt aagagctatg ctggaaacag catagcaagt
     1441 ttaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gctttttttc
     1501 tcgagtacta ggatccatta ggctttccca tgattccttc atatttgcat atacgataca
     1561 aggctgttag agagataatt ggaattaatt tgactgtaaa cacaaagata ttagtacaaa
     1621 atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta
     1681 aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg gctttatata
     1741 tcttgtggaa aggacgaaac accgtaaaat aacagactac tccggtttaa gagctatgct
     1801 ggaaacagca tagcaagttt aaataaggct agtccgttat caacttgaaa aagtggcacc
     1861 gagtcggtgc tttttttctc gagtactagg atccattagg ctttcccatg attccttcat
     1921 atttgcatat acgatacaag gctgttagag agataattgg aattaatttg actgtaaaca
     1981 caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag
     2041 ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg
     2101 atttcttggc tttatatatc ttgtggaaag gacgaaacac cgtttttttt ctagaggtac
     2161 ccgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     2221 tgacgtcaat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     2281 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     2341 acgtcaatga cggtaaatgg cccgcctggc attgtgccca gtacatgacc ttatgggact
     2401 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc
     2461 ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat
     2521 ttatttttta attattttgt gcagcgatgg gggcgggggg gggggggggg gggggcgcgc
     2581 gccaggcggg gcggggcggg gcgaggggcg gggcggggcg aggcggagag gtgcggcggc
     2641 agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg
     2701 gccctataaa aagcgaagcg cgcggcgggc gggagtcgct gcgcgctgcc ttcgccccgt
     2761 gccccgctcc gccgccgcct cgcgccgccc gccccggctc tgactgaccg cgttactccc
     2821 acaggtgagc gggcgggacg gcccttctcc tccgggctgt aattagctga gcaagaggta
     2881 agggtttaag ggatggttgg ttggtggggt attaatgttt aattacctgg agcacctgcc
     2941 tgaaatcact ttttttcagg ttggaccggt gccaccatga gcgagctgat taaggagaac
     3001 atgcacatga agctgtacat ggagggcacc gtggacaacc atcacttcaa gtgcacatcc
     3061 gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt ggtcgagggc
     3121 ggccctctcc ccttcgcctt cgacatcctg gctactagct tcctctacgg cagcaagacc
     3181 ttcatcaacc acacccaggg catccccgac ttcttcaagc agtccttccc tgagggcttc
     3241 acatgggaga gagtcaccac atacgatgac gggggcgtgc tgaccgctac ccaggacacc
     3301 agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa cttcacatcc
     3361 aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccttcaccga gacgctgtac
     3421 cccgctgacg gcggcctgga aggcagaaac gacatggccc tgaagctcgt gggcgggagc
     3481 catctgatcg caaacatcaa gaccacatat agatccaaga aacccgctaa gaacctcaag
     3541 atgcctggcg tctactatgt ggactacaga ctggaaagaa tcaaggaggc caacaacgaa
     3601 acctacgtcg agcagcacga ggtggcagtg gccagatact gcgacctccc tagcaaactg
     3661 gggcacaagc ttaattaaga attcctagag ctcgctgatc agcctcgact gtgccttcta
     3721 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     3781 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     3841 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagagaata
     3901 gcaggcatgc tggggagctc tcgaagccgc ggtgcgggtg ccagggcgtg cccttgggct
     3961 ccccgggcgc gtactccacc tcacccatcg caggaacccc tagtgatgga gttggccact
     4021 ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg
     4081 ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg
     4141 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac
     4201 catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     4261 tgaccgctac acttgccagc gccttagcgc ccgctccttt cgctttcttc ccttcctttc
     4321 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     4381 gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta
     4441 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     4501 atagtggact cttgttccaa actggaacaa cactcaactc tatctcgggc tattcttttg
     4561 atttataagg gattttgccg atttcggtct attggttaaa aaatgagctg atttaacaaa
     4621 aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta
     4681 caatctgctc tgatgccgca tagtgctttt cccaaggcag tctggagcat gcgctttagc
     4741 agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc
     4801 caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc
     4861 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat
     4921 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg
     4981 ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag
     5041 gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc
     5101 ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct
     5161 gttctcctct tcctcatctc cgggcctttc gaccgatcat caagctgcac gtgttgacaa
     5221 ttaatcatcg gcatagtata tcggcatagt ataatacgac tcactatagg agggccacca
     5281 tgggatcggc cattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     5341 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     5401 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     5461 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     5521 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     5581 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     5641 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     5701 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     5761 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
     5821 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     5881 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     5941 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     6001 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     6061 gccttcttga cgagttcttc tgatcgagaa attgatgatc tattaaacaa taaagacgtc
     6121 cacatggaag tgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     6181 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     6241 tggctggttt attgctgata aatctggagc cggtgagcgt ggaagccgcg gtatcattgc
     6301 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     6361 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     6421 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     6481 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     6541 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttc
//
