LOCUS       pEPMS0CM0031_(TkTXSS-pT 4348 bp ds-DNA     circular     15-MAY-2023
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name: TkTKSS.gb TkTKSS in
            pTwist Chlor High Copy Resistance markers: Chloramphenicol This
            file was created using tools provided by Twist Bioscience
FEATURES             Location/Qualifiers
     source          1..4348
                     /label="source"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             1..2335
                     /label="TkTXSS"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             14..2320
                     /label="Taraxasterol synthase"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature_   2336..4348
                     /label="vector_backbone-2335-4348"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature_   2392..2407
                     /label="m13_reverse"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature_   2580..3151
                     /label="colE1_high_copy"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature_   3301..3394
                     /label="lambda_t0_terminator"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature_   3416..4061
                     /label="CAT"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature_   4076..4177
                     /label="CAT_promoter"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature_   4280..4294
                     /label="m13_forward"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
ORIGIN
        1 TAGGTCTCTA ATGTGGAAGC TGAGAATAGG TGAAAAGAAT GGAAAGTTCA ACATTGGTGA
       61 TGGAAACGGT GATGACTATT TGCATAGCAC CAACAACTTT GTGGGGAGAC AAACATGGGA
      121 ATTCGACCCT GATGCAGGCA CGCAGAAAGA GCGTGACGAA ATTGAAAGGC TTCGAGAGCA
      181 ATTTTTGATC AATAAAAAGA AGCTTGACAT TAGATGTTGC GCAGACTTGC TAATGCGAAA
      241 TCAGCTTATT AAGGAAAGCG GAATTGATCT TATAAGCGTA CCTCCAGTGA GACTTGGTGA
      301 CGACGAAGAT GTGAACTATG AAGCGGTGAC AACAGCCGTG AGAAAGGCAG TTAGACTGAA
      361 CCGTGCGATT CAAGCTTGGG ATGGCCATTG GCCAGCTGAA AATGCCGGTC CGTTGTTCTT
      421 CACTCCTCCC TTGATAATTG CCCTGTACAT AAGTGGTACA TTGGATACCA TCCTCACACA
      481 AGAACACAAA AGGGAGATGA TTCGTTATAT GTACATCCAT CAAAATGAGG ACGGAGGGTG
      541 GGGGTTCTAT ATATCAGGCA GAAGCACAAT GATAGGGACT GCGCTGAATT ATGTGGGTCT
      601 AAGACTTCTT GGAGAAGATG ATAATGATGC AATTGCTAAA GGCCGTAAAT GGATACTTGA
      661 CCACGGTGGC GCCACCTCTA TTCCTTCGTG GGGAAAGGTG TATCTTTCGG TGCTTGGAGT
      721 GTACGAATGG GCAGGGTGTA ACCCTCTTCC TCCAGAATTC TGGCTTTTTC CTTCCTTTTT
      781 GCCTTATCAT CCAGCAAAAA TGTGGTGTTA TTGCAGAACC ACATACATGC CAATGTCATA
      841 CTTGTATGGT ACAGGCTTCC AAGGGCCTAT TACGGACCTT GTTAAGTCTT TGAGAAAAGA
      901 GATTCATGTC ATTCCTTACC ATCAGATTGA TTGGAACAAA CAACGACATA ATTGTTGCAA
      961 GGAGGATCTT TACTACCCTC ATACCTACAT CCAAGATCTC TTATGGGATG GACTTCATTA
     1021 TTTTAGTGAG CCACTTGTAT CAAAATGGCC TCTCAAGAAG TTAAGAGAGA AGGGTTTGAA
     1081 AAGAGTATTG GATCTAATGC AATATAACGC CGAGGAAGGA CGTTACATAA CTCTGGGTTG
     1141 CGTTGAGAAG TCTTTACAAA TGATGTGTTT TTCTGCCCTA GATCCAAATG GAATTGACTT
     1201 TAAACGACAC CTTGCTAGAG TCCCCGATTA CTTATGGGTG GCGGAGGATG GTATGAAAAT
     1261 GCAAAGTTTT GGTAGTCAGT TATGGGATTG TACTCTTGTA ACTCAAGCAA TTATCGCTAG
     1321 TGATATGGTT GAAGAATATG GAGATTCACT TAAAAAAGCT AACTTTTACT TAAAAGAATC
     1381 ACAGATCAAA CAAAACCCAA AAGGCGATTT CGAAAACATG TGTCGCCAGT TTACAAAAGG
     1441 GGCTTGGACT TTCTCTGATC AAGATCAAGG TTGGGTCGTT TCAGATTGCA CTGCCGAAGC
     1501 TGTGAAGTGT TTATTGGCAT TGTCACAAAT GCCACAAGAA ATTTCCGGTG AAAAGGTCGA
     1561 CGTCGAGCGA TTATATGATG CCATTAACGT CCTTCTTTAC CTACAGAGTC CAGAAACTGG
     1621 TGGTTTTGCA ATTTGGGAGG CACCGGTTCC AAAACCATAT TTAGAGAAAT TGAATCCTTC
     1681 AGAACTTTTT GCAGACATAG TGGTCGAGAG AGAGCATGTT GAATGTACGG GGTCCATAAT
     1741 TCAAACGTTG CAAACATTTA AAACTCTGCA TCCAGGGCAT CGTCAAAAAG AAATAGAAGT
     1801 TGCTATTGAA AAAGGCATAC GCTTTTTGGA AAATAGACAA CAAGAAAATG GTTCTTGGTA
     1861 TGGTTATTGG GGTATATGTT ATCTTTATGG AACCTATTTC GTGTTACAAG GATTGGTAGC
     1921 TTGTGGACAA ACATATGAAA ATAGTGAAGC AGTTCGGAAA GCTGTCAAGT TTTTCCTCTC
     1981 GACACAAAAC TCAGAAGGTG GTTGGGGAGA GAACTTTGAG TCATGCCCAC AAGAGAAATT
     2041 TATACCTTTG GAAGGGAACA GAACAAATTT TGTGCAAACT TCATGGGCCA TGCTTGGACT
     2101 TCTATGTGGT GGACAGGCTG AAAGAGATCC GACACCATTA CACAAGGCAG CAAAATTGCT
     2161 CATAAATGGA CAAATGGATA ATGGAGACTT TCCTCAACAG GAAATAACAG GAGTGTACAT
     2221 GAAGAATTGC ATGTTACATT ATGCAGAATA TAGGAACACT TTTCCGCTAT GGGCGCTTGG
     2281 AGAGTACCGT AAGCGTGTTT GGTTAGCAAA ACAAGAAACC GGTTCGAGAG ACCTAAGGCT
     2341 AGGTGGAGGC TCAGTGATGA TAAGTCTGCG ATGGTGGATG CATGTGTCAT GGTCATAGCT
     2401 GTTTCCTGTG TGAAATTGTT ATCCGCTCAG AGGGCACAAT CCTATTCCGC GCTATCCGAC
     2461 AATCTCCAAG ACATTAGGTG GAGTTCAGTT CGGCGTATGG CATATGTCGC TGGAAAGAAC
     2521 ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT
     2581 TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG
     2641 CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC
     2701 TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC
     2761 GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC
     2821 AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC
     2881 TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT
     2941 AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT
     3001 AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC
     3061 TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT
     3121 TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG
     3181 ATCTTTTCTA CGGGGTCTGA CGCTCTATTC AACAAAGCCG CCGTCCCGTC AAGTCAGCGT
     3241 AAATGGGTAG GGGGCTTCAA ATCGTCCCCC CATACGATAT AAGTTGTTAC TAGTGCTTGG
     3301 ATTCTCACCA ATAAAAAACG CCCGGCGGCA ACCGAGCGTT CTGAACAAAT CCAGATGGAG
     3361 TTCTGAGGTC ATTACTGGAT CTATCAACAG GAGTCCAAGC GAGCTCGATA TCAAATTACG
     3421 CCCCGCCCTG CCACTCATCG CAGTACTGTT GTAATTCATT AAGCATTCTG CCGACATGGA
     3481 AGCCATCACA AACGGCATGA TGAACCTGAA TCGCCAGCGG CATCAGCACC TTGTCGCCTT
     3541 GCGTATAATA TTTGCCCATG GTGAAAACGG GGGCGAAGAA GTTGTCCATA TTGGCCACGT
     3601 TTAAATCAAA ACTGGTGAAA CTCACCCAGG GATTGGCTGA GACGAAAAAC ATATTCTCAA
     3661 TAAACCCTTT AGGGAAATAG GCCAGGTTTT CACCGTAACA CGCCACATCT TGCGAATATA
     3721 TGTGTAGAAA CTGCCGGAAA TCGTCGTGGT ATTCACTCCA GAGCGATGAA AACGTTTCAG
     3781 TTTGCTCATG GAAAACGGTG TAACAAGGGT GAACACTATC CCATATCACC AGCTCACCGT
     3841 CTTTCATTGC CATACGAAAT TCCGGATGAG CATTCATCAG GCGGGCAAGA ATGTGAATAA
     3901 AGGCCGGATA AAACTTGTGC TTATTTTTCT TTACGGTCTT TAAAAAGGCC GTAATATCCA
     3961 GCTGAACGGT CTGGTTATAG GTACATTGAG CAACTGACTG AAATGCCTCA AAATGTTCTT
     4021 TACGATGCCA TTGGGATATA TCAACGGTGG TATATCCAGT GATTTTTTTC TCCATTTTAG
     4081 CTTCCTTAGC TCCTGAAAAT CTCGATAACT CAAAAAATAC GCCCGGTAGT GATCTTATTT
     4141 CATTATGGTG AAAGTTGGAA CCTCTTACGT GCCGATCAAC GTCTCATTGA TACCTGAAAC
     4201 AAAACCCATC GTACGGCCAA GGAAGTCTCC AATAACTGTG ATCCACCACA AGCGCCAGGG
     4261 TTTTCCCAGT CACGACGTTG TAAAACGACG GCCAGTCATG CATAATCCGC ACGCATCTGG
     4321 AATAAGGAAG TGCCATTCCG CCTGACCT
//