LOCUS       pEPDG1CB0028_(CYP716A39 3475 bp ds-DNA     circular     05-SEP-2024
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name: CoTKSS.gb CoTKSS in
            pTwist Chlor High Copy Resistance markers: Chloramphenicol This
            file was created using tools provided by Twist Bioscience
FEATURES             Location/Qualifiers
     misc_feature    1..1462
                     /label="CaCYP1 (CYP716A392a)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature_   1463..3475
                     /label="vector_backbone-2353-4366"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     source          1463..3475
                     /label="source"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature_   1519..1534
                     /label="m13_reverse"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature_   1707..2278
                     /label="colE1_high_copy"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature_   2428..2521
                     /label="lambda_t0_terminator"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature_   2438..2471
                     /label="lambda_T0_terminator"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature_   2543..3188
                     /label="CAT"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature_   3203..3304
                     /label="CAT_promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature_   3407..3421
                     /label="m13_forward"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
ORIGIN
        1 TAGGTCTCTA ATGGAGATAT TCTATGCTTC TCTTATCTCT CTTTTTGTTC TTGTTGTTTC
       61 TTTTTCTCTT CATTTTTTCT TCTACAATTC AAAACAACCC GAAGATCAGA AACTACTTCC
      121 ACCGGGTAGA ACGGGCTGGC CCATAATCGG AGAAAGTCTC GAGTTTCTCG GAAGCGGTTG
      181 GTACGGGCAC CCCGAAAAGT TCATACTTGA CCGAATGTCA AAGTTTTCGC CTATAGTATT
      241 TAGGTCATCT CTCTTGCTAG AGGATGCTGC AGTGATGTGT GGTGCTCAAG GGAACAAATT
      301 CTTGTTCTCA AATGAGAACA AACTAGTCCA AGTATGGTGG CCCGCTTCGG TTGACAAAAT
      361 CTTTCCGTCG GCTAACAAAA CCTACAAAAC CGAAACCATT AAAATGAGAA AAATGCTCCC
      421 GAATTTCCTC AAACCCGAAG CCTTGCAACG ATATATTCCA ATCATGGATA TTACCACACA
      481 AAAACACTTT GCCGCGGGAT GGGAAGGGAA AAAAGAAGTT GTGACTTATG AACTCACAAG
      541 ACACTTTGCT TTTTGGCTTG CGTGCAAGAT CTTTCTAGAT ATCGATGATC CTGAACGGGT
      601 CAGATATTTA TCTGGCCCGT TTGAGAGCAT CGTTACGGGG ATCCTGTCGA TCCCCATTGA
      661 TCTTCCGGGG ACACCGTTTA GAAGAGGGAT CAATGGGTCC AACTTCATAA TAAAGGAACT
      721 TGTGGATATT GTGAAGCAAA GGAAAATTGA TTTGGCTCAA GGGAAAGCAA CACCAACCCA
      781 AGATATATTG TCACATATGA TTCTATTTAG CGACGAAGAT GGCGAGTTCA TGACCGAACA
      841 TGACATTTCG GGCAAGATTC TTGCGTTGCT TATTGGTGGG CATGACACTG CGAGCTCTGC
      901 TACGGCTTTT ATTGTTAAGT ATCTTGCCGA ATTGCCAGAA ATCTATGATG GAGTCTACAA
      961 CGAACAAATG GAAATTGCAA AATATAAAGC AGCTGGGGAA TTATTGAATT GGGAGGATCT
     1021 ATCAAAGATG AAATACTCAT GGAAAGTTGC ATGTGAAGTT CTTAGATTAG TCCCACCGGC
     1081 CCAAGGTGCT TTTAGAGAAG CCCTTACAGA TTTCATGTAC AATGGTTACT CTATCCCCAA
     1141 GGGCTGGAAG TTATATTGGA GTGCAAGCTC AACCCATAAA AACCCCGAGG TTTTCCCCGA
     1201 ACCCGAAAAA TTTGACCCGT CAAGATTTGA TGGCAGCGGG CCAGCACCTT ACACGTTCGT
     1261 GCCATTTGGA GGAGGCCCGC ACATGTGTCC CGGAAAAGAG TATGCCCGAT TAGAAATACT
     1321 CGTGTTTATG CATCATCTTG TAACAAAGTT CAAATGGGAG AAAATTATTC CCGATGAGCC
     1381 CGTTGTTTTC AACCCGATGC CTAGCCCTGC CAAGGGTTTT CCTATTCGTA TTTACCCTCA
     1441 TAAAGCTGGT TCGAGAGACC TAAGGCTAGG TGGAGGCTCA GTGATGATAA GTCTGCGATG
     1501 GTGGATGCAT GTGTCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCAGAGG
     1561 GCACAATCCT ATTCCGCGCT ATCCGACAAT CTCCAAGACA TTAGGTGGAG TTCAGTTCGG
     1621 CGTATGGCAT ATGTCGCTGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC
     1681 CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC
     1741 AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG
     1801 TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC
     1861 CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT
     1921 CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG
     1981 CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC
     2041 TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT
     2101 GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT
     2161 ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC
     2221 AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA
     2281 AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCTATTCAAC
     2341 AAAGCCGCCG TCCCGTCAAG TCAGCGTAAA TGGGTAGGGG GCTTCAAATC GTCCCCCCAT
     2401 ACGATATAAG TTGTTACTAG TGCTTGGATT CTCACCAATA AAAAACGCCC GGCGGCAACC
     2461 GAGCGTTCTG AACAAATCCA GATGGAGTTC TGAGGTCATT ACTGGATCTA TCAACAGGAG
     2521 TCCAAGCGAG CTCGATATCA AATTACGCCC CGCCCTGCCA CTCATCGCAG TACTGTTGTA
     2581 ATTCATTAAG CATTCTGCCG ACATGGAAGC CATCACAAAC GGCATGATGA ACCTGAATCG
     2641 CCAGCGGCAT CAGCACCTTG TCGCCTTGCG TATAATATTT GCCCATGGTG AAAACGGGGG
     2701 CGAAGAAGTT GTCCATATTG GCCACGTTTA AATCAAAACT GGTGAAACTC ACCCAGGGAT
     2761 TGGCTGAGAC GAAAAACATA TTCTCAATAA ACCCTTTAGG GAAATAGGCC AGGTTTTCAC
     2821 CGTAACACGC CACATCTTGC GAATATATGT GTAGAAACTG CCGGAAATCG TCGTGGTATT
     2881 CACTCCAGAG CGATGAAAAC GTTTCAGTTT GCTCATGGAA AACGGTGTAA CAAGGGTGAA
     2941 CACTATCCCA TATCACCAGC TCACCGTCTT TCATTGCCAT ACGAAATTCC GGATGAGCAT
     3001 TCATCAGGCG GGCAAGAATG TGAATAAAGG CCGGATAAAA CTTGTGCTTA TTTTTCTTTA
     3061 CGGTCTTTAA AAAGGCCGTA ATATCCAGCT GAACGGTCTG GTTATAGGTA CATTGAGCAA
     3121 CTGACTGAAA TGCCTCAAAA TGTTCTTTAC GATGCCATTG GGATATATCA ACGGTGGTAT
     3181 ATCCAGTGAT TTTTTTCTCC ATTTTAGCTT CCTTAGCTCC TGAAAATCTC GATAACTCAA
     3241 AAAATACGCC CGGTAGTGAT CTTATTTCAT TATGGTGAAA GTTGGAACCT CTTACGTGCC
     3301 GATCAACGTC TCATTGATAC CTGAAACAAA ACCCATCGTA CGGCCAAGGA AGTCTCCAAT
     3361 AACTGTGATC CACCACAAGC GCCAGGGTTT TCCCAGTCAC GACGTTGTAA AACGACGGCC
     3421 AGTCATGCAT AATCCGCACG CATCTGGAAT AAGGAAGTGC CATTCCGCCT GACCT
//