LOCUS       pEPDG1CB0026_(CoACT3-pT 3091 bp ds-DNA     circular     05-SEP-2024
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name: CoTKSS.gb CoTKSS in
            pTwist Chlor High Copy Resistance markers: Chloramphenicol This
            file was created using tools provided by Twist Bioscience
FEATURES             Location/Qualifiers
     Editing Hist... 1..9
                     /label="GGATAAACTAAAATAATATACTAATAATAAAAATTGGATGTCGGGAGTAATAAGTAAATACAAATATTGGTTCGAAGAGACTAGATTGAAGATGCTAGC"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    1..1078
                     /label="CoACT3"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     extracted re... 2..10
                     /label="Extracted region from CoTXSS*"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     BsaI site       3..8
                     /label="BsaI site"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     Base insertion  9..9
                     /label="misc_feature"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     Mol Clo fusi... 10..10
                     /label="AATG"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     Editing Hist... 717..717
                     /label="C"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     Editing Hist... 1044..1044
                     /label="C"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     Base insertion  1064..1065
                     /label="misc_feature"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     extracted re... 1064..1078
                     /label="Extracted region from CcOSC1"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     Editing Hist... 1064..1078
                     /label="TGACCATGCAAATATTGAGGGTATATAAGACTTTTGACTAAGTTTGTAGAGGTAATAGAAAGTATGACAACTTTCTTTTAATATTTTTACTACATTTTGTTTCTTTTAATATTTTTCATAATATGAGATGTAATTTTACTATTGGTAATTTGGATTGGTTAGAGTAAGATTGAGTTGAGTTGAGTTGAGTTGCTAATAGACATATAAAAATTAAGCTGAAATGCTGAATTGAGCTG"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     Mol Clo fusi... 1066..1069
                     /label="TTCG"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     Base insertion  1070..1070
                     /label="misc_feature"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     BsaI site       1071..1076
                     /label="BsaI site"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature_   1079..3091
                     /label="vector_backbone-2353-4366"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     source          1079..3091
                     /label="source"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature_   1135..1150
                     /label="m13_reverse"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature_   1323..1894
                     /label="colE1_high_copy"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature_   2044..2137
                     /label="lambda_t0_terminator"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature_   2054..2087
                     /label="lambda_T0_terminator"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature_   2159..2804
                     /label="CAT"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature_   2819..2920
                     /label="CAT_promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature_   3023..3037
                     /label="m13_forward"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
ORIGIN
        1 TAGGTCTCTA ATGGAGGGAG AGATGAAAAA CATGATATTT GTATGGGCAA CAGCGTTTGG
       61 ATCTCTCTTC ATCTGCCACA AAATCACCAA AGTTACTGCA CCAGGTACAA GTCGCTTATG
      121 CGCATTCATC CCTGTGGTTT GCATCTTCTT ATACCTCCCC CTCCGGCTTA CAACTGTTCA
      181 TTTCGCCGGC ACAACCTCTT TCTTCATCTC TTGGCTTGCA AACTTCAAAT TGATTTTGCT
      241 TGCTTTCAAT AAAGGCCCTC TGTTTTCAAA TCCACCCCTT CCTTTCTCCA AATTCATCCT
      301 CACCGCTTGT GTTCCCATTA AAATTTCATC TCATAGATCT GATTCTGAAC CCAAAAAATC
      361 ACCGACGGCG ATCAAATACT CCCTCAAGTT TATGGTGTTT CTTCTGTTGC TTAAAGTGTA
      421 TGAATACGAC GATCAATTTC ATCCTCTCAT CAGAATGACT CTGTTTTGTG TTCATTTATA
      481 TGTGGCTCTA GACCTAGGTT TAGCCATGGT TGCATACCTG GCTCGATCCA TCACTGGATT
      541 CGAACTCGAA CCACAGTTCG ATGAACCTTA CCTGGCTACA TCTCTACAAG ACTTTTGGGG
      601 AAAGAGGTGG AATCTAATGG TCACGGGTAT ACTACACCCT GCCGTGTACC TACCCGTCCG
      661 AGCAATTTCC GGTAGGTTTC TGACCAAAAA GCGGGCCGCA CTTCCTGCTC TTATGTTTTC
      721 GTTTATCGTA TCCGGGCTGA TGCACGAGTT GATTTTTTAT TACATTGGGC GATTGAAGCC
      781 CACATGGGAA GTCACGGGGT TCTTCGTCAT TCATGGCGTG CTGGTGAGTA TGGAGGTGGT
      841 GGTAAAAAGG GCGGTAGATG GAAGATTCCA TCTGCCAGCT GCGGTTTCCA GGCCGCTGGC
      901 GTTGGGTTTG GTGATCGCCA CCAGCTTCTG GTTATTCTTT CCGCCGTTCC TGCGGTTTGA
      961 TTCGGAAGTC CGATTATGTG AAGAATTTCT CGCCTTCATG GAACTTGTCC GTCGTGGACG
     1021 GTTTGTTGGC CCAAAGGACG TCTTTTGCCC TTACTTTAGA CGCGGTTCGA GAGACCTAAG
     1081 GCTAGGTGGA GGCTCAGTGA TGATAAGTCT GCGATGGTGG ATGCATGTGT CATGGTCATA
     1141 GCTGTTTCCT GTGTGAAATT GTTATCCGCT CAGAGGGCAC AATCCTATTC CGCGCTATCC
     1201 GACAATCTCC AAGACATTAG GTGGAGTTCA GTTCGGCGTA TGGCATATGT CGCTGGAAAG
     1261 AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG
     1321 TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG
     1381 TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG
     1441 CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA
     1501 AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC
     1561 TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT
     1621 AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT
     1681 GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG
     1741 CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT
     1801 ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT
     1861 GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT
     1921 TTGATCTTTT CTACGGGGTC TGACGCTCTA TTCAACAAAG CCGCCGTCCC GTCAAGTCAG
     1981 CGTAAATGGG TAGGGGGCTT CAAATCGTCC CCCCATACGA TATAAGTTGT TACTAGTGCT
     2041 TGGATTCTCA CCAATAAAAA ACGCCCGGCG GCAACCGAGC GTTCTGAACA AATCCAGATG
     2101 GAGTTCTGAG GTCATTACTG GATCTATCAA CAGGAGTCCA AGCGAGCTCG ATATCAAATT
     2161 ACGCCCCGCC CTGCCACTCA TCGCAGTACT GTTGTAATTC ATTAAGCATT CTGCCGACAT
     2221 GGAAGCCATC ACAAACGGCA TGATGAACCT GAATCGCCAG CGGCATCAGC ACCTTGTCGC
     2281 CTTGCGTATA ATATTTGCCC ATGGTGAAAA CGGGGGCGAA GAAGTTGTCC ATATTGGCCA
     2341 CGTTTAAATC AAAACTGGTG AAACTCACCC AGGGATTGGC TGAGACGAAA AACATATTCT
     2401 CAATAAACCC TTTAGGGAAA TAGGCCAGGT TTTCACCGTA ACACGCCACA TCTTGCGAAT
     2461 ATATGTGTAG AAACTGCCGG AAATCGTCGT GGTATTCACT CCAGAGCGAT GAAAACGTTT
     2521 CAGTTTGCTC ATGGAAAACG GTGTAACAAG GGTGAACACT ATCCCATATC ACCAGCTCAC
     2581 CGTCTTTCAT TGCCATACGA AATTCCGGAT GAGCATTCAT CAGGCGGGCA AGAATGTGAA
     2641 TAAAGGCCGG ATAAAACTTG TGCTTATTTT TCTTTACGGT CTTTAAAAAG GCCGTAATAT
     2701 CCAGCTGAAC GGTCTGGTTA TAGGTACATT GAGCAACTGA CTGAAATGCC TCAAAATGTT
     2761 CTTTACGATG CCATTGGGAT ATATCAACGG TGGTATATCC AGTGATTTTT TTCTCCATTT
     2821 TAGCTTCCTT AGCTCCTGAA AATCTCGATA ACTCAAAAAA TACGCCCGGT AGTGATCTTA
     2881 TTTCATTATG GTGAAAGTTG GAACCTCTTA CGTGCCGATC AACGTCTCAT TGATACCTGA
     2941 AACAAAACCC ATCGTACGGC CAAGGAAGTC TCCAATAACT GTGATCCACC ACAAGCGCCA
     3001 GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTC ATGCATAATC CGCACGCATC
     3061 TGGAATAAGG AAGTGCCATT CCGCCTGACC T
//