LOCUS       Copy_of_pEPHS1CB0021_(T 7442 bp ds-DNA     circular     02-SEP-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     Misc._feature   complement(28..155)
                     /label="RB short"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     Misc._feature   complement(179..1660)
                     /label="RK2 trfa (no Esp3I)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     Misc._feature   1671..2460
                     /label="pUC ori"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     Replication_ori 1706..1706
                     /label="ORI"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             complement(2468..3325)
                     /label="AP r"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     Replication_ori complement(3523..4140)
                     /label="oriV"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     Misc._feature   4170..4317
                     /label="TDNA-LB"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          4236..4259
                     /label="214or (L1, REV, LB)"
                     /note="sequence: CTGGTGGCAGGATATATTGTGGTG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     Promoter        4373..4798
                     /label="CaMV 35S promoter short"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     5'_UTR          4815..4876
                     /label="TMV Omega"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             4886..7197
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MWKLRIGEKNGKFNIGDGNGDDYLHSTNNFVGRQTWEFDPDAGTQKERDEIERLREQFLINKKKLDIRCCADLLMRNQLIKESGIDLISVPPVRLGDDEDVNYEAVTTAVRKAVRLNRAIQAWDGHWPAENAGPLFFTPPLIIALYISGTLDTILTQEHKREMIRYMYIHQNEDGGWGFYISGRSTMIGTALNYVGLRLLGEDDNDAIAKGRKWILDHGGATSIPSWGKVYLSVLGVYEWAGCNPLPPEFWLFPSFLPYHPAKMWCYCRTTYMPMSYLYGTGFQGPITDLVKSLRKEIHVIPYHQIDWNKQRHNCCKEDLYYPHTYIQDLLWDGLHYFSEPLVSKWPLKKLREKGLKRVLDLMQYNAEEGRYIGLGCVEKSLQMMCFSALDPNGIDFKRHLARVPDYLWVAEDGMKMQSFGSQLWDCTLVTQAIIASDMVEEYGDSLKKANFYLKESQIKQNPKGDFENMCRQFTKGAWTFSDQDQGWVVSDCTAEAVKCLLALSQMPQEISGEKVDVERLYDAINVLLYLQSPETGGFAIWEAPVPKPYLEKLNPSELFADIVVEREHVECTGSIIQTLQTFKTLHPGHRQKEIEVAIEKGIRFLENRQQENGSWYGYWGICYLYGTYFVLQGLVACGQTYENSEAVRKAVKFFLSTQNSEGGWGENFESCPQEKFIPLEGNRTNFVQTSWAMLGLLCGGQAERDPTPLHKAAKLLINGQMDNGDFPQQEITGVYMKNCMLHYAEYRNTFPLWALGEYRKRVWLAKQET"
     CDS             4889..7195
                     /label="Taraxasterol synthase"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    6493..6493
                     /label="G"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    7195..7195
                     /label="C"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     Base_insertion  7196..7197
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    7202..7204
                     /label="stop codon"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     Terminator      7235..7438
                     /label="35S terminator (CaMV)"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
ORIGIN
        1 GCAATTGTCT TCTGCACGAA GTGGTTTAAA CTATCAGTGT TTGACAGGAT ATATTGGCGG
       61 GTAAACCTAA GAGAAAAGAG CGTTTATTAG AATAATCGGA TATTTAAAAG GGCGTGAAAA
      121 GGTTTATCCG TTCGTCCATT TGTATGTGCA TGCCAACCAC AGGGTTCCCC AGATCAGGCG
      181 CTGGCTGCTG AACCCCCAGC CGGAACTGAC CCCACAAGGC CCTAGCGTTT GCAATGCACC
      241 AGGTCATCAT TGACCCAGGC GTGTTCCACC AGGCCGCTGC CTCGCAACTC TTCGCAGGCT
      301 TCGCCGACCT GCTCGCGCCA CTTCTTCACG CGGGTGGAAT CCGATCCGCA CATGAGGCGG
      361 AAGGTTTCCA GCTTGAGCGG GTACGGCTCC CGGTGCGAGC TGAAATAGTC GAACATCCGT
      421 CGGGCCGTCG GCGACAGCTT GCGGTACTTC TCCCATATGA ATTTCGTGTA GTGGTCGCCA
      481 GCAAACAGCA CGACGATTTC CTCGTCGATC AGGACCTGGC AACGGGACGT TTTCTTGCCA
      541 CGGTCCAGGA CGCGGAAGCG GTGCAGCAGC GACACCGATT CCAGGTGCCC AACGCGGTCG
      601 GACGTGAAGC CCATCGCCGT CGCCTGTAGG CGCGACAGGC ATTCCTCGGC CTTCGTGTAA
      661 TACCGGCCAT TGATCGACCA GCCCAGGTCC TGGCAAAGCT CGTAGAACGT GAAGGTGATC
      721 GGCTCGCCGA TAGGGGTGCG CTTCGCGTAC TCCAACACCT GCTGCCACAC CAGTTCGTCA
      781 TCGTCGGCCC GCAGCTCGAC GCCGGTGTAG GTGATCTTCA CGTCCTTGTT GACGTGGAAA
      841 ATGACCTTGT TTTGCAGCGC CTCGCGCGGG ATTTTCTTGT TGCGCGTGGT GAACAGGGCA
      901 GAGCGGGCCG TGTCGTTTGG CATCGCTCGC ATCGTGTCCG GCCACGGCGC AATATCGAAC
      961 AAGGAAAGCT GCATTTCCTT GATCTGCTGC TTCGTGTGTT TCAGCAACGC GGCCTGCTTG
     1021 GCCTCGCTGA CCTGTTTTGC CAGGTCCTCG CCGGCGGTTT TTCGCTTCTT GGTCGTCATA
     1081 GTTCCTCGCG TGTCGATGGT CATCGACTTC GCCAAACCTG CCGCCTCCTG TTCAAGACGA
     1141 CGCGAACGCT CCACGGCGGC CGATGGCGCG GGCAGGGCAG GGGGAGCCAG TTGCACGCTG
     1201 TCGCGCTCGA TCTTGGCCGT AGCTTGCTGG ACCATCGAGC CGACGGACTG GAAGGTTTCG
     1261 CGGGGCGCAC GCATGACGGT GCGGCTTGCG ATGGTTTCGG CATCCTCGGC GGAAAACCCC
     1321 GCGTCGATCA GTTCTTGCCT GTATGCCTTC CGGTCAAACG TCCGATTCAT TCACCCTCCT
     1381 TGCGGGATTG CCCCGACTCA CGCCGGGGCA ATGTGCCCTT ATTCCTGATT TGACCCGCCT
     1441 GGTGCCTTGG TGTCCAGATA ATCCACCTTA TCGGCAATGA AGTCGGTCCC GTAGACCGTC
     1501 TGGCCGTCCT TCTCGTACTT GGTATTCCGA ATCTTGCCCT GCACGAATAC CAGCGACCCC
     1561 TTGCCCAAAT ACTTGCCGTG GGCCTCGGCC TGAGAGCCAA AACACTTGAT GCGGAAGAAG
     1621 TCGGTGCGCT CCTGCTTGTC GCCGGCATCG TTGCGCCACA TCTAGGATCT GCCAGGAACC
     1681 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA
     1741 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT
     1801 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC
     1861 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC
     1921 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC
     1981 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT
     2041 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG
     2101 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA
     2161 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA
     2221 AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA
     2281 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG
     2341 AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC
     2401 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG
     2461 ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT
     2521 CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG
     2581 GCCCCAGTGC TGCAATGATA CCGCGAGAAC CACGCTCACC GGCTCCAGAT TTATCAGCAA
     2641 TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA
     2701 TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC
     2761 GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT
     2821 CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA
     2881 AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT
     2941 CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT
     3001 TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA
     3061 GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG
     3121 TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA
     3181 GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA
     3241 CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG
     3301 CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC
     3361 AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG
     3421 GGGTTCCGCG CACGAATTGG CCAGCGCTGC CATTTTTGGG GTGAGGCCGT TCGCGGCCGA
     3481 GGGGCGCAGC CCCTGGGGGG ATGGGAGGCC CGCGTTAGCG GGCCGGGAGG GTTCGAGAAG
     3541 GGGGGGCACC CCCCTTCGGC GTGCGCGGTC ACGCGCACAG GGCGCAGCCC TGGTTAAAAA
     3601 CAAGGTTTAT AAATATTGGT TTAAAAGCAG GTTAAAAGAC AGGTTAGCGG TGGCCGAAAA
     3661 ACGGGCGGAA ACCCTTGCAA ATGCTGGATT TTCTGCCTGT GGACAGCCCC TCAAATGTCA
     3721 ATAGGTGCGC CCCTCATCTG TCAGCACTCT GCCCCTCAAG TGTCAAGGAT CGCGCCCCTC
     3781 ATCTGTCAGT AGTCGCGCCC CTCAAGTGTC AATACCGCAG GGCACTTATC CCCAGGCTTG
     3841 TCCACATCAT CTGTGGGAAA CTCGCGTAAA ATCAGGCGTT TTCGCCGATT TGCGAGGCTG
     3901 GCCAGCTCCA CGTCGCCGGC CGAAATCGAG CCTGCCCCTC ATCTGTCAAC GCCGCGCCGG
     3961 GTGAGTCGGC CCCTCAAGTG TCAACGTCCG CCCCTCATCT GTCAGTGAGG GCCAAGTTTT
     4021 CCGCGAGGTA TCCACAACGC CGGCGGCCGC GGTGTCTCGC ACACGGCTTC GACGGCGTTT
     4081 CTGGCGCGTT TGCAGGGCCA TAGACGGCCG CCAGCCCAGC GGCGAGGGCA ACCAGCCCGG
     4141 TGAGCGTCGC AAAGGAGATC CTGATCTGAC TGATGGGCTG CCTGTATCGA GTGGTGATTT
     4201 TGTGCCGAGC TGCCGGTCGG GGAGCTGTTG GCTGGCTGGT GGCAGGATAT ATTGTGGTGT
     4261 AAACAAATTG ACGCTTAGAC AACTTAATAA CACATTGCGG ACGTTTTTAA TGTACTGGGG
     4321 TGGATGCAGT GGGCCCCACT CTGTGAAGAC AATGCCGAAT TCGGATCCGG AGGTCAACAT
     4381 GGTGGAGCAC GACACTCTGG TCTACTCCAA AAATGTCAAA GATACAGTCT CAGAAGATCA
     4441 AAGGGCTATT GAGACTTTTC AACAAAGGAT AATTTCGGGA AACCTCCTCG GATTCCATTG
     4501 CCCAGCTATC TGTCACTTCA TCGAAAGGAC AGTAGAAAAG GAAGGTGGCT CCTACAAATG
     4561 CCATCATTGC GATAAAGGAA AGGCTATCAT TCAAGATCTC TCTGCCGACA GTGGTCCCAA
     4621 AGATGGACCC CCACCCACGA GGAGCATCGT GGAAAAAGAA GAGGTTCCAA CCACGTCTAC
     4681 AAAGCAAGTG GATTGATGTG ACATCTCCAC TGACGTAAGG GATGACGCAC AATCCCACTA
     4741 TCCTTCGCAA GACCCTTCCT CTATATAAGG AAGTTCATTT CATTTGGAGA GGACACGCTC
     4801 GAGTATAAGA GCTCATTTTT ACAACAATTA CCAACAACAA CAAACAACAA ACAACATTAC
     4861 AATTACATTT ACAATTATCG ATACAATGtg gaaactcagg attggcgaaa aaaacggcaa
     4921 atttaacatc ggtgacggga acggagatga ctatctgcac agtactaaca acttcgtcgg
     4981 tcgtcaaacg tgggaattcg atccagacgc cggaacgcaa aaggagcgag atgaaataga
     5041 acgtcttagg gaacagttcc tgataaataa aaaaaaattg gatatcaggt gctgcgccga
     5101 cttattaatg aggaaccaac tcatcaaaga aagtggaata gatttaatca gtgtaccacc
     5161 cgttcgttta ggggatgatg aggacgtaaa ttatgaagca gtgacgacag ccgttcgtaa
     5221 agctgtgcgt ctgaatagag ctatacaggc ctgggacgga cattggcccg ctgaaaatgc
     5281 tggcccgtta ttcttcactc cgccattaat tatagcattg tacatctccg ggaccttaga
     5341 tactatactg actcaagagc acaagcgaga aatgatccga tatatgtata tacaccaaaa
     5401 tgaagatgga ggctggggat tttacatatc cgggcgtagc actatgatag ggacagcact
     5461 caattacgtc ggactgaggt tactgggcga agatgataat gatgccattg caaagggtcg
     5521 aaagtggatc cttgatcacg gaggggcaac atcaattcca agttggggca aggtttacct
     5581 ctccgtgctt ggcgtctacg aatgggctgg ctgtaaccca ttaccaccag agttttggtt
     5641 gttccccagt tttctcccct accacccagc taagatgtgg tgttattgtc gaactaccta
     5701 catgccgatg agctacctct acgggacagg attccagggt cccataactg atctggtaaa
     5761 atctctgagg aaagagattc atgttatccc ttaccaccag attgactgga ataaacaaag
     5821 acacaactgt tgtaaagaag atctgtacta tccgcataca tacatacagg acctcttgtg
     5881 ggatgggtta cattacttct ctgagcccct cgttagtaag tggcctctta aaaagttacg
     5941 tgagaagggc ttgaagaggg tccttgactt gatgcaatat aacgccgaag aaggtagata
     6001 cataggattg gggtgtgtcg agaaatcctt acaaatgatg tgcttctcag ctcttgaccc
     6061 aaatggtatc gattttaaaa gacacctggc ccgagtgcct gactacctct gggtcgctga
     6121 ggacgggatg aagatgcaat ctttcgggag tcagttgtgg gactgtacgc tggtaacgca
     6181 agcaataatc gcctccgaca tggtcgaaga atatggcgac tctttaaaga aagctaattt
     6241 ctacttaaaa gagtcacaaa taaagcaaaa ccccaagggg gactttgaga atatgtgtag
     6301 gcaatttacc aaaggagctt ggacatttag cgaccaggac caaggctggg ttgttagtga
     6361 ttgcactgct gaagcagtta aatgcctgct cgctttgtct cagatgcctc aggaaatttc
     6421 tggtgagaaa gtggacgttg agcgattgta tgacgctatt aacgtccttt tgtatttgca
     6481 aagtccagag actggggggt tcgccatctg ggaagctccg gtgcctaagc catacctcga
     6541 aaagctcaac ccctctgaac tttttgctga catagtagta gaacgtgagc acgtagagtg
     6601 cacagggagt ataattcaga ctctccagac tttcaagaca ctgcacccgg gccatcgtca
     6661 gaaagaaatc gaggtggcta ttgagaaagg aatcaggttt ctcgagaata ggcagcaaga
     6721 gaacggatca tggtacggat attggggtat atgttacttg tacggaacgt atttcgtgct
     6781 gcaaggactg gtggcctgtg gacagacgta tgagaactcc gaagccgtta gaaaagctgt
     6841 taaatttttc ctttcaacac aaaattcaga gggtggctgg ggagaaaatt tcgagtcttg
     6901 ccctcaggaa aaatttatcc cacttgaggg aaaccgaaca aattttgtac agacctcttg
     6961 ggccatgtta gggttgctgt gtggagggca agcagaaagg gatccgacac ctcttcacaa
     7021 agctgctaag ctgcttatta atggtcaaat ggacaacggg gacttccctc agcaagaaat
     7081 cactggggtt tatatgaaga attgcatgct gcactacgcc gaatatcgaa acaccttccc
     7141 tttatgggct ttaggcgaat accgtaaacg agtgtggctg gcaaaacaag agactggttc
     7201 gtaagtcgac cggctgctaa caaagcccga GCTTCTCTAG CTAGAGTCGA TCGACAAGCT
     7261 CGAGTTTCTC CATAATAATG TGTGAGTAGT TCCCAGATAA GGGAATTAGG GTTCCTATAG
     7321 GGTTTCGCTC ATGTGTTGAG CATATAAGAA ACCCTTAGTA TGTATTTGTA TTTGTAAAAT
     7381 ACTTCTATCA ATAAAATTTC TAATTCCTAA AACCAAAATC CAGTACTAAA ATCCAGATCG
     7441 CT
//