LOCUS       pRel-Hyg               11389 bp    DNA     circular UNA 12-JUL-2023
DEFINITION  pRel-Hyg.
ACCESSION   urn.local...qs-g0tnwcv
VERSION     urn.local...qs-g0tnwcv
KEYWORDS    .
SOURCE      other DNA
  ORGANISM  other DNA.
FEATURES             Location/Qualifiers
     CDS             423..1865
                     /codon_start=1
                     /transl_table=11
                     /product="the rolling circle replication initiator protein
                     Rep"
                     /translation="MWKPGEATWGNTCRCNNVHTCPWCMSRILAVRGSNVQLAADGLA
                     DAGYGLHLGTNTLRHFERMAFGTVRKGMRHGLVAVLHDGWKGAYGSSGRRWRTMRDDF
                     GIIGYERAFEDTFGWGSGWHLHWHTLWVTREVLGPDAQAAFRDALAGAWAAGVESAGG
                     YTVSETCDRPGCSCEGKGHGTDVRPLNGADAADGDAGKQARYLYKDGDKTKGGVAKIG
                     LELAGQNFKAGRGDDRMGPLDLGDAAAAELQRLRRPGPFVEKYREREFGVFQVRKHYR
                     SQNLNRLIKELGIQQDVRTEEEITDDTEGLVAIAVIPAYIWYRYIARVAGRRLDLIKV
                     AETYGLPGVRRLVESWGLVWGKDVLDPPAPEAPAAPGDLDADQMRFEVMSEEEAAFRE
                     ARRKANEARTEELAASLDRVRQPKKEAIRPTISLRKRLKPKPVTVDVKTPPPGAASPV
                     CRRCKGKLAPVLQPWGQHPGDCLRVDTAVA"
                     /standard_name="Rep(pSG5)"
     misc_feature    423..1865
                     /note="Geneious type: Origin of replication"
                     /gene="rep(pSG5)"
                     /note="Geneious type: gene"
                     /standard_name="rep(pSG5)"
     misc_feature    2530..2778
                     /note="Geneious type: Origin of replication"
                     /note="Geneious type: oriT"
                     /standard_name="oriT"
     CDS             3063..4061
                     /codon_start=1
                     /transl_table=11
                     /product="hygromycin resistant protein"
                     /translation="MTQESLLLLDRIDSDDSYASLRNDQEFWEPLARRALEELGLPVP
                     PVLRVPGESTNPVLVGEPDPVIKLFGEHWCGPESLASESEAYAVLADAPVPVPRLLGR
                     GELRPGTGAWPWPYLVMSRMTGTTWRSAMDGTTDRNALLALARELGRVLGRLHRVPLT
                     GNTVLTPHSEVFPELLRERRAATVEDHRGWGYLSPRLLDRLEDWLPDVDTLLAGREPR
                     FVHGDLHGTNIFVDLAATEVTGIVDFTDVYAGDSRYSLVQLHLNAFRGDREILAALLD
                     GAQWKRTEDFARELLAFTFLHDFEVFEETPLDLSGFTDPEELAQFLWGPPDTAPGA"
                     /standard_name="HygR"
     gene            3063..4061
                     /standard_name="hygR"
     misc_feature    4430..4929
                     /note="Geneious type: Origin of replication"
                     /note="Geneious type: rep_origin"
                     /standard_name="rep(pBR322)"
     regulatory      5502..5615
                     /note="Geneious type: promoter"
                     /standard_name="PermE*"
     misc_feature    5622..5703
                     /note="gRNA scaffold; guide RNA scaffold for the
                     Streptococcus pyogenes CRISPR/Cas9 system"
                     /standard_name="gRNA scaffold"
     regulatory      5716..5886
                     /note="Geneious type: terminator"
                     /standard_name="to"
     CDS             6021..6881
                     /codon_start=1
                     /transl_table=11
                     /product="Ampicillin resistant protein"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /standard_name="AmpR"
     gene            6021..6881
                     /gene="ampR"
                     /standard_name="bla"
     regulatory      7012..7111
                     /note="Geneious type: promoter"
                     /standard_name="PtipA"
     CDS             7147..11253
                     /codon_start=1
                     /transl_table=11
                     /product="Cas9"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIK
                     KNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
                     ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAH
                     MIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSK
                     SRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLD
                     NLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTL
                     LKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVK
                     LNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY
                     YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVL
                     PKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKE
                     DYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLT
                     LFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDF
                     LKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQT
                     VKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKE
                     HPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKV
                     LTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKA
                     GFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIG
                     KATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
                     MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVV
                     AKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLF
                     ELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQH
                     KHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGA
                     PAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
                     /standard_name="Cas9"
     gene            7147..11253
                     /standard_name="cas9"
     regulatory      11260..11312
                     /note="Geneious type: Terminator"
                     /note="Geneious type: terminator"
                     /standard_name="Term1"
ORIGIN      
        1 tttgctctgt gacgagcgta cgagagaaca gcgcgtcgac accaccgcgc cccgccggac
       61 cgaagtgcca ctgcggcacc ccgatcgtcc acatccccgg gaagcgcccc aaaatctact
      121 gctcggacgc ctgccgaaag cgcgcaaagc gtctcattgc ggaccagtca cgcagcgtag
      181 caagcacgac tacagcgggt aatccggacg cgggctttag tagtaaggga aaatctccgt
      241 ccactgcagg tcagagcgct gtaactggaa cgggttctac cccaaacgag ggtgccgcga
      301 cagccgggcg ggaggacccg cgcgacgagc ggttcgcccg gcgggaccgg caccagacgg
      361 tgtcgctgaa cgaggcgttc aagggctgcg ggacccggct cactgccggg acggccgagc
      421 tgatgtggaa gccgggcgag gcgacgtggg gcaacacgtg ccgctgcaac aacgtccaca
      481 cctgcccgtg gtgcatgagc cgcatcctgg ccgtacgggg ctcgaacgtg cagctcgccg
      541 ccgacggtct cgcggacgcc gggtacgggc tgcaccttgg cacgaacacg ttgcgccact
      601 tcgagcgcat ggcgttcggc accgtccgca agggaatgcg gcacggcctg gtcgcggtct
      661 tgcacgacgg ctggaagggc gcgtacggct cgtccggccg ccgctggcgc acgatgcgcg
      721 acgacttcgg gatcatcggc tacgagcggg ccttcgagga caccttcggc tggggctccg
      781 gctggcacct gcactggcac acgctctggg tcacccgcga ggtgctcggc ccggacgccc
      841 aggccgcgtt ccgcgacgca ctcgccggag cctgggccgc cggggtcgaa tccgcgggcg
      901 gctacaccgt cagcgagacg tgcgaccggc ccggttgctc ctgtgagggc aagggccacg
      961 gcaccgacgt gcgcccactc aacggcgcgg acgccgctga cggcgatgcg ggcaagcagg
     1021 cccgctacct ctacaaggac ggcgacaaga ccaagggcgg cgtcgccaag atcggcctgg
     1081 agctggccgg acagaacttc aaggccggtc gcggcgacga ccgcatggga ccgctcgacc
     1141 tgggcgacgc ggcggccgcc gagctgcagc ggcttcgtcg gcccggcccg ttcgtcgaga
     1201 agtaccgcga gcgcgagttc ggcgtcttcc aggtccgcaa gcactaccgg tcgcagaacc
     1261 tgaaccggct gatcaaggaa ctcggcatcc agcaggacgt ccgcaccgag gaggagatca
     1321 ccgacgacac cgagggcctg gtcgccatcg cggtcatccc ggcctacatc tggtaccgat
     1381 acatcgcccg cgtcgccgga cgtcgcctcg acctcatcaa ggtcgccgag acgtacggcc
     1441 taccgggcgt ccggcggctc gtcgagtcct ggggcctggt gtggggcaag gacgtcctgg
     1501 acccgcccgc cccggaagcg ccggccgcgc cgggcgacct tgacgctgac cagatgaggt
     1561 tcgaggtcat gtccgaggag gaagctgctt tccgcgaggc gcgccggaag gccaacgagg
     1621 cccgcacgga ggagctggcc gcttcgctcg accgagtgcg tcagccgaag aaggaggcga
     1681 tccgcccgac gatcagcctt cggaagcgcc tgaagccgaa gccggtcacc gtcgacgtca
     1741 agactccccc gcccggcgcc gcgtcgcccg tgtgccgccg ctgcaagggc aagctcgccc
     1801 cggtactcca gccctggggc cagcaccccg gagactgcct ccgcgtcgac acagcggtcg
     1861 cgtagggccg ctgagggacc gccatgagac cgccaccccg gacatccggg tggcggtctc
     1921 atggcggtcc cggcgcgaaa gccgagggtc tgcctgccgt gaggtggccg gcggacctgg
     1981 gttactgctg gaatcccgcg cccagtccga gcgtccgccg gcgcttttca cctggctccg
     2041 ggtgctcgtc gatgagcacc aggccgtcga cgacgacgcg tccgcctgcc tgcttttggc
     2101 cggcgggccg ggccgcggtt gtccgccggg tcggaccggt cccttggtcg accgatgcct
     2161 cggccacgac gatgctgggc ccgaagcggt acgcgacccg tgccgggccg cagcggatcg
     2221 cggcagcagg agctccccgc ccgccacggc tggctggatg gtcgcctcac gcgcccgcag
     2281 gggtgacggc gtgggtgcgg cggtcagccc cgtgatcggg cgtcagacgg ccgctgaggg
     2341 accgcgacca cccctccctt cccgcctgcc gtctttcacc gcccggccgg tccgcagacg
     2401 ccgtacggcc aggccacgga ggattacggg cagatggcgc ccacccggca cccgggctgg
     2461 ggcaccctgg acagcacgaa ggtccgcccc actcaccggg gcgggccttc gcgttgagct
     2521 agagttcccg ccagcctcgc agagcaggat tcccgttgag caccgccagg tgcgaataag
     2581 ggacagtgaa gaaggaacac ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct
     2641 gacgccgttg gatacaccaa ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc
     2701 gtgcgaaaaa ggatggatat accgaaaaaa tcgctataat gaccccgaag cagggttatg
     2761 cagcggaaaa tgcagctcac ggtaactgat gccgtatttg cagtaccagc gtacggccca
     2821 cagaatgatg tcacgctgaa aatgccggcc tttgaatggg ttcatgtgca gctccataag
     2881 caaaagggga tgataagttt atcaccaccg actatttgca atgtaggctg gagctgcttc
     2941 gatttaaata atacttgaca tatcactgtg attcacatat aatatgcgca ggtcggctgg
     3001 ttggctgaca aagtgcgtag gatgactgta ctacaatcaa aaaaataata aggaggtaaa
     3061 tcatgaccca ggagtccctg ctgctgctgg accgcatcga cagcgacgac agctacgcga
     3121 gcctgcgcaa cgaccaggag ttctgggagc cgctcgcccg ccgcgcgctg gaagagctgg
     3181 gcctgccggt cccgccggtg ctgcgcgtgc cgggtgagtc caccaacccg gtgctggtgg
     3241 gcgagccgga cccggtcatc aagctgttcg gggagcactg gtgcggcccg gagagcctgg
     3301 cgagcgagag cgaggcgtac gcggtgctgg ccgacgcccc ggtcccggtg ccgcgcctgc
     3361 tgggtcgcgg cgagctgcgc ccaggcacgg gtgcctggcc gtggccgtac ctggtgatgt
     3421 cccgcatgac gggcaccacg tggcgcagcg cgatggacgg caccaccgac cgcaacgccc
     3481 tgctggccct ggcgcgcgaa ctgggtcgcg tcctgggtcg cctgcatcgc gtcccgctga
     3541 ccggcaacac ggtgctgacc ccgcactccg aagtgttccc ggagctgctg cgcgagcgcc
     3601 gcgccgccac cgtcgaggac caccggggct ggggctacct gagcccgcgc ctgctggacc
     3661 gcctggaaga ctggctgccg gacgtcgaca cgctgctggc gggtcgcgag ccgcgcttcg
     3721 tgcacggcga cctgcacggc accaacatct tcgtcgacct ggcggccacc gaggtcaccg
     3781 gcatcgtcga cttcacggac gtgtacgccg gtgactcccg ctactccctg gtccagcttc
     3841 atctgaacgc cttccgcggt gaccgcgaga tcctggcggc gctgctggac ggtgcgcagt
     3901 ggaagcgcac cgaggacttc gcgcgcgagc tgctggcctt cacgttcctc cacgacttcg
     3961 aggtcttcga agaaaccccg ctggacctga gcggcttcac cgacccggaa gaactggcgc
     4021 agttcctgtg gggtccgccg gacacggcgc cgggcgcgtg ataagatagc tcatgagcgg
     4081 agaacgagat gacgttggag gggcaaggtc gcgctgattg ctggggcaac acgtggagcg
     4141 gatcggggat tgtctttctt cagctcgctg atgatatgct gacgctcaat gccgtagttc
     4201 ctattcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
     4261 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     4321 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaaaaggat ctaggtgaag
     4381 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     4441 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     4501 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     4561 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     4621 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     4681 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     4741 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     4801 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     4861 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     4921 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     4981 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     5041 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     5101 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     5161 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     5221 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     5281 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     5341 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     5401 cggctcgtat gttgtgtgga attgtgagtt taaacgcgga taacaatttc acacagtcga
     5461 cttctagtgc agctagtgtc gactagagga tccccgggta tgcggtcgat cttgacggct
     5521 ggcgagaggt gcggggagga tctgaccgac gcggtccaca cgtggcaccg cgatgctgtt
     5581 gtgggcacaa tcgtgccggt tggtaggatc gacggccatg ggttttaggt ctagaaatag
     5641 caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt
     5701 ttttacgtag gcgtcagcta cgatgttccg gggactgctg atccggtcag caggtggaag
     5761 agggactgga ttccaaagtt ctcaatgctg cttgctgttc ttgaatgggg ggtcgttgac
     5821 gacgacatgg ctcgattggc gcgacaagtt gctgcgattc tcaccaataa aaaacgcccg
     5881 gcggcagaac aaatccagat ggagttcact agttgcgcgg aacccctatt tgtttatttt
     5941 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     6001 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     6061 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     6121 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     6181 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     6241 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     6301 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     6361 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     6421 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     6481 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     6541 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     6601 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     6661 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     6721 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     6781 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     6841 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     6901 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     6961 tcctttttga taatctcatg accaaaatgc tagctgaggt cattactgga ccggatcggg
     7021 gatctgggct gagggagccg acggcacgcg gcggctcacg gcgtggcacg cggaacgtcc
     7081 gggcttgcac ctcacgtcac gtgaggaggc agcgtggacg gcgtcagaga agggagcgga
     7141 caattgatgg acaagaagta ctccatcggc ctggacatcg gcaccaactc cgtcggctgg
     7201 gccgtcatca ccgacgagta caaggtcccg tccaagaagt tcaaggtcct gggcaacacc
     7261 gaccgccact ccatcaagaa gaacctgatc ggcgccctgc tgttcgactc cggcgagacc
     7321 gccgaggcca cccgcctgaa gcgcaccgcc cgccgccgct acacccgccg caagaaccgc
     7381 atctgctacc tgcaggagat cttctccaac gagatggcca aggtcgacga ctccttcttc
     7441 caccgcctgg aggagtcctt cctggtcgag gaggacaaga agcacgagcg ccacccgatc
     7501 ttcggcaaca tcgtcgacga ggtcgcctac cacgagaagt acccgaccat ctaccacctg
     7561 cgcaagaagc tggtcgactc caccgacaag gccgacctgc gcctgatcta cctggccctg
     7621 gcccacatga tcaagttccg cggccacttc ctgatcgagg gcgacctgaa cccggacaac
     7681 tccgacgtcg acaagctgtt catccagctg gtccagacct acaaccagct gttcgaggag
     7741 aacccgatca acgcctccgg cgtcgacgcc aaggccatcc tgtccgcccg cctgtccaag
     7801 tcccgccgcc tggagaacct gatcgcccag ctgccgggcg agaagaagaa cggcctgttc
     7861 ggcaacctga tcgccctgtc cctgggcctg accccgaact tcaagtccaa cttcgacctg
     7921 gccgaggacg ccaagctgca gctgtccaag gacacctacg acgacgacct ggacaacctg
     7981 ctggcccaga tcggcgacca gtacgccgac ctgttcctgg ccgccaagaa cctgtccgac
     8041 gccatcctgc tgtccgacat cctgcgcgtc aacaccgaga tcaccaaggc cccgctgtcc
     8101 gcctccatga tcaagcgcta cgacgagcac caccaggacc tgaccctgct gaaggccctg
     8161 gtccgccagc agctgccgga gaagtacaag gagatcttct tcgaccagtc caagaacggc
     8221 tacgccggct acatcgacgg cggcgcctcc caggaggagt tctacaagtt catcaagccg
     8281 atcctggaga agatggacgg caccgaggag ctgctggtca agctgaaccg cgaggacctg
     8341 ctgcgcaagc agcgcacctt cgacaacggc tccatcccgc accagatcca cctgggcgag
     8401 ctgcacgcca tcctgcgccg ccaggaggac ttctacccgt tcctgaagga caaccgcgag
     8461 aagatcgaga agatcctgac cttccgcatc ccgtactacg tcggcccgct ggcccgcggc
     8521 aactcccgct tcgcctggat gacccgcaag tccgaggaga ccatcacccc gtggaacttc
     8581 gaggaggtcg tcgacaaggg cgcctccgcc cagtccttca tcgagcgcat gaccaacttc
     8641 gacaagaacc tgccgaacga gaaggtcctg ccgaagcact ccctgctgta cgagtacttc
     8701 accgtctaca acgagctgac caaggtcaag tacgtcaccg agggcatgcg caagccggcc
     8761 ttcctgtccg gcgagcagaa gaaggccatc gtcgacctgc tgttcaagac caaccgcaag
     8821 gtcaccgtca agcagctgaa ggaggactac ttcaagaaga tcgagtgctt cgactccgtc
     8881 gagatctccg gcgtcgagga ccgcttcaac gcctccctgg gcacctacca cgacctgctg
     8941 aagatcatca aggacaagga cttcctggac aacgaggaga acgaggacat cctggaggac
     9001 atcgtcctga ccctgaccct gttcgaggac cgcgagatga tcgaggagcg cctgaagacc
     9061 tacgcccacc tgttcgacga caaggtcatg aagcagctga agcgccgccg ctacaccggc
     9121 tggggccgcc tgtcccgcaa gctgatcaac ggcatccgcg acaagcagtc cggcaagacc
     9181 atcctggact tcctgaagtc cgacggcttc gccaaccgca acttcatgca gctgatccac
     9241 gacgactccc tgaccttcaa ggaggacatc cagaaggccc aggtctccgg ccagggcgac
     9301 tccctgcacg agcacatcgc caacctggcc ggctccccgg ccatcaagaa gggcatcctg
     9361 cagaccgtca aggtcgtcga cgagctggtc aaggtcatgg gccgccacaa gccggagaac
     9421 atcgtcatcg agatggcccg cgagaaccag accacccaga agggccagaa gaactcccgc
     9481 gagcgcatga agcgcatcga ggagggcatc aaggagctgg gctcccagat cctgaaggag
     9541 cacccggtcg agaacaccca gctgcagaac gagaagctgt acctgtacta cctgcagaac
     9601 ggccgcgaca tgtacgtcga ccaggagctg gacatcaacc gcctgtccga ctacgacgtc
     9661 gaccacatcg tcccgcagtc cttcctgaag gacgactcca tcgacaacaa ggtcctgacc
     9721 cgctccgaca agaaccgcgg caagtccgac aacgtcccgt ccgaggaggt cgtcaagaag
     9781 atgaagaact actggcgcca gctgctgaac gccaagctga tcacccagcg caagttcgac
     9841 aacctgacca aggccgagcg cggcggcctg tccgagctgg acaaggccgg cttcatcaag
     9901 cgccagctgg tcgagacccg ccagatcacc aagcacgtcg cccagatcct ggactcccgc
     9961 atgaacacca agtacgacga gaacgacaag ctgatccgcg aggtcaaggt catcaccctg
    10021 aagtccaagc tggtctccga cttccgcaag gacttccagt tctacaaggt ccgcgagatc
    10081 aacaactacc accacgccca cgacgcctac ctgaacgccg tcgtcggcac cgccctgatc
    10141 aagaagtacc cgaagctgga gtccgagttc gtctacggcg actacaaggt ctacgacgtc
    10201 cgcaagatga tcgccaagtc cgagcaggag atcggcaagg ccaccgccaa gtacttcttc
    10261 tactccaaca tcatgaactt cttcaagacc gagatcaccc tggccaacgg cgagatccgc
    10321 aagcgcccgc tgatcgagac caacggcgag accggcgaga tcgtctggga caagggccgc
    10381 gacttcgcca ccgtccgcaa ggtcctgtcc atgccgcagg tcaacatcgt caagaagacc
    10441 gaggtccaga ccggcggctt ctccaaggag tccatcctgc cgaagcgcaa ctccgacaag
    10501 ctgatcgccc gcaagaagga ctgggacccg aagaagtacg gcggcttcga ctccccgacc
    10561 gtcgcctact ccgtcctggt cgtcgccaag gtcgagaagg gcaagtccaa gaagctgaag
    10621 tccgtcaagg agctgctggg catcaccatc atggagcgct cctccttcga gaagaacccg
    10681 atcgacttcc tggaggccaa gggctacaag gaggtcaaga aggacctgat catcaagctg
    10741 ccgaagtact ccctgttcga gctggagaac ggccgcaagc gcatgctggc ctccgccggc
    10801 gagctgcaga agggcaacga gctggccctg ccgtccaagt acgtcaactt cctgtacctg
    10861 gcctcccact acgagaagct gaagggctcc ccggaggaca acgagcagaa gcagctgttc
    10921 gtcgagcagc acaagcacta cctggacgag atcatcgagc agatctccga gttctccaag
    10981 cgcgtcatcc tggccgacgc caacctggac aaggtcctgt ccgcctacaa caagcaccgc
    11041 gacaagccga tccgcgagca ggccgagaac atcatccacc tgttcaccct gaccaacctg
    11101 ggcgccccgg ccgccttcaa gtacttcgac accaccatcg accgcaagcg ctacacctcc
    11161 accaaggagg tcctggacgc caccctgatc caccagtcca tcaccggcct gtacgagacc
    11221 cgcatcgacc tgtcccagct gggcggcgac taaggatcgg acgaacaata aggggagcgg
    11281 gaaaccgctc ccctttttta ttgataacaa aaaagcttct cgtcgaaggc actagaaggc
    11341 ctatatgcgg ggatcgaccg cgcagcatgc cgagtgagga cgtttaaac
//
