LOCUS       Exported                7364 bp ds-DNA     circular DNA 23-SEP-2024
DEFINITION  pRS304.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7364)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Sep 23, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7364
                     /organism="pRS304"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     misc_feature    complement(29..51)
                     /label=pGEX_3_primer
                     /note="color: #a6acb3; direction: LEFT"
     promoter        185..465
                     /label=TRP1 Promoter
                     /note="color: #ffffff"
     gene            466..1140
                     /gene="TRP1"
                     /label=TRP1
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             466..1140
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /note="color: #ff7f50"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     misc_feature    complement(1708..1856)
                     /label=lacZ_a
                     /note="/translation=MTMITPSSELTLTKGNKSWVPGPPSRSTVSISLISNSCS
                     PGDPL 
                     VLERPPPRWSSNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA 
                     RTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLAT 
                     FAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    1822..1844
                     /label=M13_pUC_fwd_primer
                     /note="/translation=MTMITPSSELTLTKGNKSWVPGPPSRSTVSISLISNSCS
                     PGDPL 
                     VLERPPPRWSSNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA 
                     RTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLAT 
                     FAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
                     /note="color: #a6acb3"
     promoter        1837..1853
                     /label=M13_forward20_primer
                     /note="/translation=MTMITPSSELTLTKGNKSWVPGPPSRSTVSISLISNSCS
                     PGDPL 
                     VLERPPPRWSSNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA 
                     RTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLAT 
                     FAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
                     /note="color: #ffffff"
     promoter        1860..1878
                     /label=T7_promoter
                     /note="/translation=MTMITPSSELTLTKGNKSWVPGPPSRSTVSISLISNSCS
                     PGDPL 
                     VLERPPPRWSSNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA 
                     RTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLAT 
                     FAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
                     /note="color: #ffffff"
     primer_bind     complement(1908..1929)
                     /label=pRS_BB_Tpm1_R
                     /note="color: #a020f0; direction: LEFT"
     misc_feature    1911..1927
                     /label=pBluescriptSK_primer
                     /note="/translation=MTMITPSSELTLTKGNKSWVPGPPSRSTVSISLISNSCS
                     PGDPL 
                     VLERPPPRWSSNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA 
                     RTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLAT 
                     FAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
                     /note="color: #a6acb3"
     promoter        1930..2575
                     /label=Abp140 Promoter
                     /note="color: #ffffff"
     gene            2576..2626
                     /label=LifeAct
                     /note="color: #a6acb3; direction: RIGHT"
     gene            2651..3358
                     /label=mNeon
                     /note="color: #a6acb3; direction: RIGHT"
     gene            3377..4084
                     /label=mNeon
                     /note="color: #a6acb3; direction: RIGHT"
     gene            4103..4810
                     /label=mNeon
                     /note="color: #a6acb3; direction: RIGHT"
     terminator      4843..5030
                     /label=ADH1 Terminator
                     /note="color: #ffffff"
     primer_bind     5029..5053
                     /label=pRS_BB_Tpm1_F
                     /note="color: #a020f0; direction: RIGHT"
     misc_feature    complement(5059..5075)
                     /label=pBluescriptKS_primer
                     /note="/translation=MTMITPSSELTLTKGNKSWVPGPPSRSTVSISLISNSCS
                     PGDPL 
                     VLERPPPRWSSNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA 
                     RTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLAT 
                     FAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
                     /note="color: #a6acb3; direction: LEFT"
     promoter        complement(5103..5122)
                     /label=T3_promoter
                     /note="/translation=MTMITPSSELTLTKGNKSWVPGPPSRSTVSISLISNSCS
                     PGDPL 
                     VLERPPPRWSSNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA 
                     RTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLAT 
                     FAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
                     /note="color: #ffffff; direction: LEFT"
     promoter        complement(5139..5157)
                     /label=M13_reverse_primer
                     /note="/translation=MTMITPSSELTLTKGNKSWVPGPPSRSTVSISLISNSCS
                     PGDPL 
                     VLERPPPRWSSNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA 
                     RTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLAT 
                     FAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
                     /note="color: #ffffff; direction: LEFT"
     misc_feature    complement(5156..5178)
                     /label=M13_pUC_rev_primer
                     /note="/translation=MTMITPSSELTLTKGNKSWVPGPPSRSTVSISLISNSCS
                     PGDPL 
                     VLERPPPRWSSNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA 
                     RTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLAT 
                     FAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
                     /note="color: #a6acb3; direction: LEFT"
     rep_origin      complement(5530..6149)
                     /direction=LEFT
                     /label=pBR322_origin
                     /note="/translation=MTMITPSSELTLTKGNKSWVPGPPSRSTVSISLISNSCS
                     PGDPL 
                     VLERPPPRWSSNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA 
                     RTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLAT 
                     FAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
                     /note="color: #ffff00"
     gene            complement(6304..7164)
                     /gene="Ampicillin"
                     /label=Ampicillin
                     /note="/translation=MTMITPSSELTLTKGNKSWVPGPPSRSTVSISLISNSCS
                     PGDPL 
                     VLERPPPRWSSNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA 
                     RTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLAT 
                     FAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
                     /note="color: #a6acb3; direction: LEFT"
     CDS             complement(6304..7164)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 6304 .. 7095 / #ccffcc
                        2: 7096 .. 7164 / #ccffcc / signal sequence
                     Cleavage site after base 7095"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7165..7269)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accaaacgac attactatat atataatata ggaagcattt aatagacagc atcgtaatat
      241 atgtgtactt tgcagttatg acgccagatg gcagtagtgg aagatattct ttattgaaaa
      301 atagcttgtc accttacgta caatcttgat ccggagcttt tctttttttg ccgattaaga
      361 attaattcgg tcgaaaaaag aaaaggagag ggccaagagg gagggcattg gtgactattg
      421 agcacgtgag tatacgtgat taagcacaca aaggcagctt ggagtatgtc tgttattaat
      481 ttcacaggta gttctggtcc attggtgaaa gtttgcggct tgcagagcac agaggccgca
      541 gaatgtgctc tagattccga tgctgacttg ctgggtatta tatgtgtgcc caatagaaag
      601 agaacaattg acccggttat tgcaaggaaa atttcaagtc ttgtaaaagc atataaaaat
      661 agttcaggca ctccgaaata cttggttggc gtgtttcgta atcaacctaa ggaggatgtt
      721 ttggctctgg tcaatgatta cggcattgat atcgtccaac tgcatggaga tgagtcgtgg
      781 caagaatacc aagagttcct cggtttgcca gttattaaaa gactcgtatt tccaaaagac
      841 tgcaacatac tactcagtgc agcttcacag aaacctcatt cgtttattcc cttgtttgat
      901 tcagaagcag gtgggacagg tgaacttttg gattggaact cgatttctga ctgggttgga
      961 aggcaagaga gccccgaaag cttacatttt atgttagctg gtggactgac gccagaaaat
     1021 gttggtgatg cgcttagatt aaatggcgtt attggtgttg atgtaagcgg aggtgtggag
     1081 acaaatggtg taaaagactc taacaaaata gcaaatttcg tcaaaaatgc taagaaatag
     1141 gttattactg agtagtattt atttaagtat tgtttgtgca cttgcctgcg gtgtgaaata
     1201 ccgcacagat gcgtaaggag aaaataccgc atcaggaaat tgtaaacgtt aatattttgt
     1261 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     1321 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     1381 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     1441 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     1501 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     1561 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     1621 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     1681 tacagggcgc gtcgcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
     1741 tgcgggcctc ttcgctatta cgccagctgg cgaagggggg atgtgctgca aggcgattaa
     1801 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattgt
     1861 aatacgactc actatagggc gaattggagc tccaccgcgg tggcggccgc tctagaacta
     1921 gtggatcccg ggtttgcgtt taagatgcca agattcatca gctgtcatgc caaggccatt
     1981 gattacccac aatcaaacat aacctacata ggaattgacc ctaaaccagc aaactacaac
     2041 caaacacaac tttcaaaata ctatgatgat ctagtacaaa tggaagataa aaatgcacta
     2101 agcttatttt cttcagattg gtacgctaca aaggatagat tactcaccaa gaaaagatct
     2161 cgcaacccat ttaacagaac tgcaccgtac gctcagaata ttttctgcaa agaaaacggc
     2221 aagaggatcg agggaatcga ggatgacgag gaatattttg aaacaaaaat taagtgtaaa
     2281 atgccatggt cgtcgccaag acaataaacg tgtgggagta ttgcaaaaac atagtatata
     2341 agcgtaaatc actatataaa gcccaaagaa gttagcacta aagcatcatt catcgttcaa
     2401 aactcctctc tcaagtgaga gaattcggcg ctgaaaaaaa gctttgtcaa aaccaagaaa
     2461 aaaacgtagg attggacacg aggttctttt tttaatgtaa acaagaagtg gcactatagc
     2521 tgtgaaactt ccaatacaat tgtataccaa ataagctatt acaaattgat ttaaaatggg
     2581 tgtcgcagat ttgatcaaga aattcgaaag catctcaaag gaagaaggtg acggtgctgg
     2641 tttaattaac atggtgagca agggcgagga ggataacatg gcctctctcc cagcgacaca
     2701 tgagttacac atctttggct ccatcaacgg tgtggacttt gacatggtgg gtcagggcac
     2761 cggcaatcca aatgatggtt atgaggagtt aaacctgaag tccaccaagg gtgacctcca
     2821 gttctccccc tggattctgg tccctcatat cgggtatggc ttccatcagt acctgcccta
     2881 ccctgacggg atgtcgcctt tccaggccgc catggtagat ggctccggct accaagtcca
     2941 tcgcacaatg cagtttgaag atggtgcctc ccttactgtt aactaccgct acacctacga
     3001 gggaagccac atcaaaggag aggcccaggt gaaggggact ggtttccctg ctgacggtcc
     3061 tgtgatgacc aactcgctga ccgctgcgga ctggtgcagg tcgaagaaga cttaccccaa
     3121 cgacaaaacc atcatcagta cctttaagtg gagttacacc actggaaatg gcaagcgcta
     3181 ccggagcact gcgcggacca cctacacctt tgccaagcca atggcggcta actatctgaa
     3241 gaaccagccg atgtacgtgt tccgtaagac ggagctcaag cactccaaga ccgagctcaa
     3301 cttcaaggag tggcaaaagg cctttaccga tgtgatgggc atggacgagc tgtacaaggg
     3361 gtctggcgga agcgggatgg tgagcaaggg cgaggaggat aacatggcct ctctcccagc
     3421 gacacatgag ttacacatct ttggctccat caacggtgtg gactttgaca tggtgggtca
     3481 gggcaccggc aatccaaatg atggttatga ggagttaaac ctgaagtcca ccaagggtga
     3541 cctccagttc tccccctgga ttctggtccc tcatatcggg tatggcttcc atcagtacct
     3601 gccctaccct gacgggatgt cgcctttcca ggccgccatg gtagatggct ccggctacca
     3661 agtccatcgc acaatgcagt ttgaagatgg tgcctccctt actgttaact accgctacac
     3721 ctacgaggga agccacatca aaggagaggc ccaggtgaag gggactggtt tccctgctga
     3781 cggtcctgtg atgaccaact cgctgaccgc tgcggactgg tgcaggtcga agaagactta
     3841 ccccaacgac aaaaccatca tcagtacctt taagtggagt tacaccactg gaaatggcaa
     3901 gcgctaccgg agcactgcgc ggaccaccta cacctttgcc aagccaatgg cggctaacta
     3961 tctgaagaac cagccgatgt acgtgttccg taagacggag ctcaagcact ccaagaccga
     4021 gctcaacttc aaggagtggc aaaaggcctt taccgatgtg atgggcatgg acgagctgta
     4081 caagtcagca ggttccgcag ggatggtgag caagggcgag gaggataaca tggcctctct
     4141 cccagcgaca catgagttac acatctttgg ctccatcaac ggtgtggact ttgacatggt
     4201 gggtcagggc accggcaatc caaatgatgg ttatgaggag ttaaacctga agtccaccaa
     4261 gggtgacctc cagttctccc cctggattct ggtccctcat atcgggtatg gcttccatca
     4321 gtacctgccc taccctgacg ggatgtcgcc tttccaggcc gccatggtag atggctccgg
     4381 ctaccaagtc catcgcacaa tgcagtttga agatggtgcc tcccttactg ttaactaccg
     4441 ctacacctac gagggaagcc acatcaaagg agaggcccag gtgaagggga ctggtttccc
     4501 tgctgacggt cctgtgatga ccaactcgct gaccgctgcg gactggtgca ggtcgaagaa
     4561 gacttacccc aacgacaaaa ccatcatcag tacctttaag tggagttaca ccactggaaa
     4621 tggcaagcgc taccggagca ctgcgcggac cacctacacc tttgccaagc caatggcggc
     4681 taactatctg aagaaccagc cgatgtacgt gttccgtaag acggagctca agcactccaa
     4741 gaccgagctc aacttcaagg agtggcaaaa ggcctttacc gatgtgatgg gcatggacga
     4801 gctgtacaag ggcagtggat cctaagcgcc acttctaaat aagcgaattt cttatgattt
     4861 atgattttta ttattaaata agttataaaa aaaataagtg tatacaaatt ttaaagtgac
     4921 tcttaggttt taaaacgaaa attcttattc ttgagtaact ctttcctgta ggtcaggttg
     4981 ctttctcagg tatagtatga ggtcgctctt attgaccaca cctctaccgg gctgcaggaa
     5041 ttcgatatca agcttatcga taccgtcgac ctcgaggggg ggcccggtac ccagcttttg
     5101 ttccctttag tgagggttaa ttccgagctt ggcgtaatca tggtcatagc tgtttcctgt
     5161 gtgaaattgt tatccgctca caattccaca caacatagga gccggaagca taaagtgtaa
     5221 agcctggggt gcctaatgag tgaggtaact cacattaatt gcgttgcgct cactgcccgc
     5281 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     5341 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     5401 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     5461 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     5521 taaaaaggcc gcgttgctgg cgtttttcca taggctcggc ccccctgacg agcatcacaa
     5581 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     5641 cccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     5701 gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct
     5761 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     5821 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     5881 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     5941 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     6001 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     6061 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     6121 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     6181 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     6241 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     6301 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     6361 catagttgcc tgactgcccg tcgtgtagat aactacgata cgggagggct taccatctgg
     6421 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     6481 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     6541 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     6601 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     6661 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgaaaaaa
     6721 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     6781 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     6841 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     6901 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     6961 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     7021 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     7081 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     7141 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     7201 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     7261 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     7321 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtc
//
