LOCUS       pRS303_7                 7548 bp    DNA     circular SYN 29-MAR-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             2910..3617
                     /note="pLannotate"
                     /label="mNeonGreen"
                     /database="fpbase"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             join(7400..7548,1..514)
                     /note="pLannotate"
                     /label="HIS3"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(801..1229)
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        complement(6697..6801)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(5836..6696)
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             2184..2891
                     /note="pLannotate"
                     /label="mNeonGreen"
                     /database="snapgene"
                     /identity="99.9"
                     /match_length="99.6"
                     /fragment="False"
                     /other="CDS"
     CDS             3636..4343
                     /note="pLannotate"
                     /label="mNeonGreen"
                     /database="snapgene"
                     /identity="99.9"
                     /match_length="99.6"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(5077..5665)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             2109..2159
                     /note="pLannotate"
                     /label="Lifeact"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        complement(4723..4753)
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    complement(4768..4789)
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        complement(4636..4654)
                     /note="pLannotate"
                     /label="T3 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        1393..1411
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     terminator      4376..4563
                     /note="pLannotate"
                     /label="ADH1 terminator"
                     /database="snapgene"
                     /identity="99.5"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     promoter        7213..7399
                     /note="pLannotate"
                     /label="HIS3 promoter"
                     /database="snapgene"
                     /identity="99.5"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    complement(4699..4715)
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             complement(1233..1390)
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="90.8"
                     /fragment="True"
                     /other="CDS"
     CDS             1463..1837
                     /note="pLannotate"
                     /label="YO238_YEAST (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="41.3"
                     /fragment="True"
                     /other="CDS"
     misc_feature    4560..4624
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="60.2"
                     /fragment="True"
                     /other="misc_feature"
     misc_feature    722..773
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="36.9"
                     /fragment="True"
                     /other="misc_feature"
     misc_feature    7033..7082
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="35.5"
                     /fragment="True"
                     /other="misc_feature"
     misc_feature    1420..1462
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="39.8"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(4802..4894)
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
     CDS             6896..6931
                     /note="pLannotate"
                     /label="rop (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="18.8"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(7083..7163)
                     /note="pLannotate"
                     /label="MRM1 (fragment)"
                     /database="swissprot"
                     /identity="96.3"
                     /match_length="6.6"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 gcaagtgatt aacgtccaca caggtatagg gtttctggac catatgatac atgctctggc
       61 caagcattcc ggctggtcgc taatcgttga gtgcattggt gacttacaca tagacgacca
      121 tcacaccact gaagactgcg ggattgctct cggtcaagct tttaaagagg ccctaggggc
      181 cgtgcgtgga gtaaaaaggt ttggatcagg atttgcgcct ttggatgagg cactttccag
      241 agcggtggta gatctttcga acaggccgta cgcagttgtc gaacttggtt tgcaaaggga
      301 gaaagtagga gatctctctt gcgagatgat cccgcatttt cttgaaagct ttgcagaggc
      361 tagcagaatt accctccacg ttgattgtct gcgaggcaag aatgatcatc accgtagtga
      421 gagtgcgttc aaggctcttg cggttgccat aagagaagcc acctcgccca atggtaccaa
      481 cgatgttccc tccaccaaag gtgttcttat gtagtgacac cgattattta aagctgcagc
      541 atacgatata tatacatgtg tatatatgta tacctatgaa tgtcagtaag tatgtatacg
      601 aacagtatga tactgaagat gacaaggtaa tgcatcattc tatacgtgtc attctgaacg
      661 aggcgcgctt tccttttttc tttttgcttt ttcttttttt ttctcttgaa ctcgacggat
      721 ctatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggaaattgt
      781 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
      841 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
      901 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
      961 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     1021 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     1081 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     1141 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     1201 cgcgcttaat gcgccgctac agggcgcgtc cattcgccat tcaggctgcg caactgttgg
     1261 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     1321 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
     1381 gccagtgaat tgtaatacga ctcactatag ggcgaattgg agctccaccg cggtggcggc
     1441 cgctctagaa ctagtggatc ccgggtttgc gtttaagatg ccaagattca tcagctgtca
     1501 tgccaaggcc attgattacc cacaatcaaa cataacctac ataggaattg accctaaacc
     1561 agcaaactac aaccaaacac aactttcaaa atactatgat gatctagtac aaatggaaga
     1621 taaaaatgca ctaagcttat tttcttcaga ttggtacgct acaaaggata gattactcac
     1681 caagaaaaga tctcgcaacc catttaacag aactgcaccg tacgctcaga atattttctg
     1741 caaagaaaac ggcaagagga tcgagggaat cgaggatgac gaggaatatt ttgaaacaaa
     1801 aattaagtgt aaaatgccat ggtcgtcgcc aagacaataa acgtgtggga gtattgcaaa
     1861 aacatagtat ataagcgtaa atcactatat aaagcccaaa gaagttagca ctaaagcatc
     1921 attcatcgtt caaaactcct ctctcaagtg agagaattcg gcgctgaaaa aaagctttgt
     1981 caaaaccaag aaaaaaacgt aggattggac acgaggttct ttttttaatg taaacaagaa
     2041 gtggcactat agctgtgaaa cttccaatac aattgtatac caaataagct attacaaatt
     2101 gatttaaaat gggtgtcgca gatttgatca agaaattcga aagcatctca aaggaagaag
     2161 gtgacggtgc tggtttaatt aacatggtga gcaagggcga ggaggataac atggcctctc
     2221 tcccagcgac acatgagtta cacatctttg gctccatcaa cggtgtggac tttgacatgg
     2281 tgggtcaggg caccggcaat ccaaatgatg gttatgagga gttaaacctg aagtccacca
     2341 agggtgacct ccagttctcc ccctggattc tggtccctca tatcgggtat ggcttccatc
     2401 agtacctgcc ctaccctgac gggatgtcgc ctttccaggc cgccatggta gatggctccg
     2461 gctaccaagt ccatcgcaca atgcagtttg aagatggtgc ctcccttact gttaactacc
     2521 gctacaccta cgagggaagc cacatcaaag gagaggccca ggtgaagggg actggtttcc
     2581 ctgctgacgg tcctgtgatg accaactcgc tgaccgctgc ggactggtgc aggtcgaaga
     2641 agacttaccc caacgacaaa accatcatca gtacctttaa gtggagttac accactggaa
     2701 atggcaagcg ctaccggagc actgcgcgga ccacctacac ctttgccaag ccaatggcgg
     2761 ctaactatct gaagaaccag ccgatgtacg tgttccgtaa gacggagctc aagcactcca
     2821 agaccgagct caacttcaag gagtggcaaa aggcctttac cgatgtgatg ggcatggacg
     2881 agctgtacaa ggggtctggc ggaagcggga tggtgagcaa gggcgaggag gataacatgg
     2941 cctctctccc agcgacacat gagttacaca tctttggctc catcaacggt gtggactttg
     3001 acatggtggg tcagggcacc ggcaatccaa atgatggtta tgaggagtta aacctgaagt
     3061 ccaccaaggg tgacctccag ttctccccct ggattctggt ccctcatatc gggtatggct
     3121 tccatcagta cctgccctac cctgacggga tgtcgccttt ccaggccgcc atggtagatg
     3181 gctccggcta ccaagtccat cgcacaatgc agtttgaaga tggtgcctcc cttactgtta
     3241 actaccgcta cacctacgag ggaagccaca tcaaaggaga ggcccaggtg aaggggactg
     3301 gtttccctgc tgacggtcct gtgatgacca actcgctgac cgctgcggac tggtgcaggt
     3361 cgaagaagac ttaccccaac gacaaaacca tcatcagtac ctttaagtgg agttacacca
     3421 ctggaaatgg caagcgctac cggagcactg cgcggaccac ctacaccttt gccaagccaa
     3481 tggcggctaa ctatctgaag aaccagccga tgtacgtgtt ccgtaagacg gagctcaagc
     3541 actccaagac cgagctcaac ttcaaggagt ggcaaaaggc ctttaccgat gtgatgggca
     3601 tggacgagct gtacaagtca gcaggttccg cagggatggt gagcaagggc gaggaggata
     3661 acatggcctc tctcccagcg acacatgagt tacacatctt tggctccatc aacggtgtgg
     3721 actttgacat ggtgggtcag ggcaccggca atccaaatga tggttatgag gagttaaacc
     3781 tgaagtccac caagggtgac ctccagttct ccccctggat tctggtccct catatcgggt
     3841 atggcttcca tcagtacctg ccctaccctg acgggatgtc gcctttccag gccgccatgg
     3901 tagatggctc cggctaccaa gtccatcgca caatgcagtt tgaagatggt gcctccctta
     3961 ctgttaacta ccgctacacc tacgagggaa gccacatcaa aggagaggcc caggtgaagg
     4021 ggactggttt ccctgctgac ggtcctgtga tgaccaactc gctgaccgct gcggactggt
     4081 gcaggtcgaa gaagacttac cccaacgaca aaaccatcat cagtaccttt aagtggagtt
     4141 acaccactgg aaatggcaag cgctaccgga gcactgcgcg gaccacctac acctttgcca
     4201 agccaatggc ggctaactat ctgaagaacc agccgatgta cgtgttccgt aagacggagc
     4261 tcaagcactc caagaccgag ctcaacttca aggagtggca aaaggccttt accgatgtga
     4321 tgggcatgga cgagctgtac aagggcagtg gatcctaagc gccacttcta aataagcgaa
     4381 tttcttatga tttatgattt ttattattaa ataagttata aaaaaaataa gtgtatacaa
     4441 attttaaagt gactcttagg ttttaaaacg aaaattctta ttcttgagta actctttcct
     4501 gtaggtcagg ttgctttctc aggtatagta tgaggtcgct cttattgacc acacctctac
     4561 cgggctgcag gaattcgata tcaagcttat cgataccgtc gacctcgagg gggggcccgg
     4621 taccagcttt tgttcccttt agtgagggtt aatttcgagc ttggcgtaat catggtcata
     4681 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     4741 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     4801 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     4861 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     4921 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     4981 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     5041 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     5101 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     5161 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     5221 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     5281 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     5341 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     5401 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     5461 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     5521 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     5581 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     5641 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     5701 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     5761 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     5821 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     5881 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     5941 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     6001 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     6061 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     6121 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     6181 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     6241 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     6301 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     6361 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     6421 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     6481 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     6541 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     6601 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     6661 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     6721 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     6781 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     6841 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
     6901 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     6961 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     7021 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
     7081 taaattcccg ttttaagagc ttggtgagcg ctaggagtca ctgccaggta tcgtttgaac
     7141 acggcattag tcagggaagt cataacacag tcctttcccg caattttctt tttctattac
     7201 tcttggcctc ctctagtaca ctctatattt ttttatgcct cggtaatgat tttcattttt
     7261 tttttccacc tagcggatga ctcttttttt ttcttagcga ttggcattat cacataatga
     7321 attatacatt atataaagta atgtgatttc ttcgaagaat atactaaaaa atgagcaggc
     7381 aagataaacg aaggcaaaga tgacagagca gaaagcccta gtaaagcgta ttacaaatga
     7441 aaccaagatt cagattgcga tctctttaaa gggtggtccc ctagcgatag agcactcgat
     7501 cttcccagaa aaagaggcag aagcagtagc agaacaggcc acacaatc
//
