LOCUS       PTet-F30-Pepper_        3067 bp    DNA     circular UNA 24-SEP-2024
DEFINITION  orignally pMK10.
ACCESSION   urn.local.zoila.1n-i6chrgf
VERSION     urn.local.zoila.1n-i6chrgf
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    1..62
                     /Source="referenceToDocument: urn:local:manis:8f-gkvsqhz"
                     /label="P9d (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     ligation        1..4
                     /label="Ligation"
     protein_bind    5..23
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes;
                     color: #31849b"
                     /Transferred_From="tet_operator, tet_operator_(2),
                     tet_operator_(3), tet_operator_(4), tet_operator_(5),
                     tet_operator_(6), tet_operator_(7), tet_operator_(9)"
                     /Transferred_Similarity="100.00%"
                     /label="tet operator"
     promoter        24..58
                     /Color="#ffffff"
                     /Transferred_From="pTet"
                     /Transferred_Similarity="100.00%"
                     /label="pTet"
     protein_bind    30..48
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes;
                     color: #31849b"
                     /Transferred_From="tet_operator, tet_operator_(2),
                     tet_operator_(3), tet_operator_(4), tet_operator_(5),
                     tet_operator_(6), tet_operator_(7), tet_operator_(9)"
                     /Transferred_Similarity="100.00%"
                     /label="tet operator"
     ligation        59..62
                     /label="Ligation"
     misc_feature    59..202
                     /Source="referenceToDocument: urn:local:manis:20-gwy1q0c"
                     /label="U27m (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     mRNA            63..163
                     /created_by="manis"
                     /modified_by="manis"
                     /label="F30-Pepper aptamer"
     mRNA            63..104
                     /created_by="manis"
                     /label="F30 Scaffold 1"
     mRNA            105..147
                     /created_by="manis"
                     /label="Pepper aptamer"
     mRNA            148..163
                     /created_by="manis"
                     /label="F30 Scaffold 2"
     5'UTR           164..198
                     /created_by="manis"
                     /label="UTR1"
     ligation        199..202
                     /label="Ligation"
     misc_feature    199..956
                     /Source="referenceToDocument: urn:local:Conference
                     Room:52-go0zyad"
                     /label="C184m (concatenated sequence 3)"
                     /note="Geneious type: Concatenated sequence"
     CDS             <203..952
                     /created_by="manis"
                     /label="mTurquiose2-GGGGS-His6x"
     ligation        953..956
                     /label="Ligation"
     misc_feature    953..1050
                     /Source="referenceToDocument: urn:local:manis:93-gkvssip"
                     /label="T12m (concatenated sequence 4)"
                     /note="Geneious type: Concatenated sequence"
     terminator      957..1046
                     /created_by="anandhswaminathan"
                     /modified_by="anandhswaminathan"
                     /label="ECK120029600 (strongest natural Voigt, 378x)"
     ligation        1047..1050
                     /label="Ligation"
     misc_feature    1047..4
                     /Source="referenceToDocument: urn:local:manis:9v-gkvsxif"
                     /label="v37m (DVCK-mScar-AE) (concatenated sequence 5)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    1059..1079
                     /note="universal suffix for all parts"
                     /label="BioBrick suffix"
     terminator      1080..1137
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during
                     synthesis. Its efficiency has not been determined."
                     /label="his operon terminator"
     primer_bind     complement(1211..1231)
                     /Sequence="TTACCGCCTTTGAGTGAGCTG"
                     /Hairpin_Tm="None"
                     /Tm="60.6"
                     /Self_Dimer_Tm="None"
                     /%GC="52.4"
                     /created_by="primer3"
                     /label="SC190 / Part.Seq.REV"
     CDS             1397..2212
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /note="confers resistance to kanamycin in bacteria or G418
                      (Geneticin(R)) in eukaryotes"
                     /label="KanR"
     misc_feature    2327..2336
                     /label="Origin"
     rep_origin      complement(2334..2922)
                     /direction="LEFT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /label="ori"
     misc_feature    complement(2337..2886)
                     /label="RNA II transcript"
     promoter        2740..2769
                     /label="RNA I promoter"
     misc_feature    2776..2883
                     /label="RNA I transcript"
     terminator      2854..2884
                     /label="RNAI Terminator"
     promoter        complement(2894..2922)
                     /label="RNAII promoter"
     misc_feature    2907..2913
                     /label="between -10 and -35 of RNA II promoter"
     primer_bind     2962..2982
                     /Sequence="AAATAGGCGTATCACGAGGCA"
                     /Hairpin_Tm="46.1"
                     /Tm="59.6"
                     /Self_Dimer_Tm="2.4"
                     /%GC="47.6"
                     /created_by="primer3"
                     /label="SC189 / Part.Seq.FOR"
     terminator      2985..3028
                     /note="putative bacterial transcription terminator"
                     /label="bacterial terminator"
     misc_feature    3031..3052
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
                     /label="BioBrick prefix"
ORIGIN      
        1 ggagtcccta tcagtgatag agattgacat ccctatcagt gatagagata ctgagcacta
       61 ctttgccatg tgtatgtggg ttcgcccaca tactctgatg atccccaatc gtggcgtgtc
      121 ggcctgcttc ggcaggcact ggcgccggga tcattcatgg caaaataatt ttgtttaact
      181 ttaagaagga gatataccaa tggtaagtaa gggcgaagag ttgtttactg gggtagtccc
      241 gatcctggtc gagctggatg gggacgttaa cggccataaa tttagcgtgt cgggtgaagg
      301 cgagggggac gcgacctacg ggaaactgac cttgaagttt atctgtacga ccgggaaact
      361 tccggtgcca tggcctacac tcgtcacgac cttgagctgg ggtgttcaat gctttgcacg
      421 ttacccagac catatgaaac agcatgattt cttcaaaagt gcaatgccag aagggtatgt
      481 tcaagagcgc accatctttt ttaaggacga tggcaactat aagacccgtg ccgaagtcaa
      541 gttcgaaggt gacacccttg tcaaccgtat cgagttgaaa ggtatcgact ttaaggaaga
      601 tgggaatatc ctgggtcata aacttgaata taattacttt tccgacaatg tttatatcac
      661 agccgacaaa caaaaaaacg gtatcaaggc caatttcaag attcggcata acatcgaaga
      721 tgggggggtt caactcgctg atcattacca gcagaataca cctatcggcg acggcccagt
      781 cttattacct gataatcatt accttagcac ccaatccaaa ctcagcaagg atccaaacga
      841 aaagcgcgat cacatggtgc ttttggagtt tgtcactgct gctggtatta cattgggtat
      901 ggatgaattg tacaaagggg gcggcggtag ccatcatcat catcaccatt aaaggtttca
      961 gccaaaaaac ttaagaccgc cggtcttgtc cactaccttg cagtaatgcg gtggacagga
     1021 tcggcggttt tcttttctct tctcaagctt atgtcttcta ctagtagcgg ccgctgcagt
     1081 ccggcaaaaa agggcaaggt gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta
     1141 cttcgcgtta tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     1201 cgagcggtat cagctcactc aaaggcggta agtcgcgtgt tatacgcccg ttatccatgg
     1261 gtatggacag ttttcccttt gatatgtaac gcacgttgtg tctcaaaatc tctgatgtta
     1321 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag
     1381 taatacaagg ggtgttatga gccatattca acgggaaacg tcttgctccc gtccgcgctt
     1441 aaactccaac atggacgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca
     1501 atcaggtgcg acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa
     1561 acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtccgtc tcaactggct
     1621 gacggagttt atgcctctcc cgaccatcaa gcattttatc cgtactcctg atgatgcgtg
     1681 gttactcacc accgcgattc ctgggaaaac agccttccag gtattagaag aatatcctga
     1741 ttcaggtgaa aatattgttg atgcgctggc cgtgttcctg cgccggttac attcgattcc
     1801 tgtttgtaat tgtcctttta acagcgatcg tgtatttcgt cttgctcagg cgcaatcacg
     1861 catgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt
     1921 tgaacaagtc tggaaagaaa tgcacaagct cttgccattc tcaccggatt cagtcgtcac
     1981 tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat
     2041 tgatgttgga cgggtcggaa tcgcagaccg ttaccaggac cttgccattc tttggaactg
     2101 cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa
     2161 tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct aataatacta
     2221 gcagaaatca tccttagcga aagctaagga ttttttttat ctgattaccg cctttgagtg
     2281 agcgtcgacc tagtcctagc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     2341 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     2401 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     2461 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     2521 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     2581 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     2641 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     2701 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     2761 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     2821 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     2881 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     2941 tctacggggt ctgacgcgca taaataggcg tatcacgagg cagaatttca gataaaaaaa
     3001 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agactagtgg
     3061 aagacat
//
