LOCUS       PT7-alphaHL             3152 bp    DNA     circular UNA 24-SEP-2024
DEFINITION  originally pZJQ.03.
ACCESSION   urn.local.zoila.n-i8vw88h
VERSION     urn.local.zoila.n-i8vw88h
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     primer_bind     40..60
                     /Sequence="AAATAGGCGTATCACGAGGCA"
                     /Hairpin_Tm="46.1"
                     /Tm="59.6"
                     /Self_Dimer_Tm="2.4"
                     /%GC="47.6"
                     /created_by="primer3"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Part.Seq.FOR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Part.Seq.FOR"
                     /label="Part.Seq.FOR"
     terminator      complement(63..106)
                     /Color="#ffffff"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="bacterial_terminator"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     bacterial terminator (bacterial_terminator)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     bacterial terminator (bacterial_terminator)"
                     /label="bacterial terminator"
     misc_feature    109..128
                     /Color="#99ccff"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="BioBrick_prefix"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; BioBrick
                     prefix (BioBrick_prefix)"
                     /label="BioBrick prefix"
     promoter        150..168
                     /Color="#ffffff"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="T7_promoter"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; T7
                     promoter (T7_promoter)"
                     /label="T7 promoter"
     RBS             177..260
                     /Color="#ffcc99"
                     /modified_by="Andrey"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CIDAR_BCD2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; CIDAR
                     BCD2 - 4.38x (<a
                     href=""urn:local:Andrey:73-9mysn1h"">CIDAR_BCD2</a>)"
                     /label="CIDAR BCD2 - 4.38x"
     gene            265..1224
                     /created_by="bruce"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C148m"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; aHL (<a
                     href=""urn:local:zoila:p-dp5d99t"">C148m</a>)"
                     /label="aHL"
     terminator      1229..1276
                     /Color="#ffffff"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="T7_terminator"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; T7
                     terminator (T7_terminator)"
                     /label="T7 terminator"
     misc_feature    1289..1309
                     /Color="#a6ccff"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="BioBrick_suffix"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; BioBrick
                     suffix (BioBrick_suffix)"
                     /label="BioBrick suffix"
     terminator      1310..1367
                     /Color="#ffffff"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="his_operon_terminator"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; his
                     operon terminator (his_operon_terminator)"
                     /label="his operon terminator"
     primer_bind     complement(1441..1461)
                     /Sequence="TTACCGCCTTTGAGTGAGCTG"
                     /Hairpin_Tm="None"
                     /Tm="60.6"
                     /Self_Dimer_Tm="None"
                     /%GC="52.4"
                     /created_by="primer3"
                     /modified_by="andrey"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Part.Seq.REV"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Part.Seq.REV"
                     /label="Part.Seq.REV"
     CDS             1627..2442
                     /Color="#ccffcc"
                     /Transferred_Translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDN
                     VGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRT
                     PDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFR
                     LAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIF
                     DEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFH
                     LMLDEFF"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR_(6)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; KanR
                     (KanR_(6))"
                     /label="KanR"
     primer_bind     complement(1630..1650)
                     /Sequence="CGTTTCCCGTTGAATATGGCT"
                     /Hairpin_Tm="31.7"
                     /Tm="59.0"
                     /Self_Dimer_Tm="None"
                     /%GC="47.6"
                     /created_by="primer3"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ASS_KanR.REV"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; KanR.REV
                     (ASS_KanR.REV)"
                     /label="KanR.REV"
     partial         complement(2535..3113)
                     /direction="RIGHT"
                     /note="color: #ffff99"
                     /Color="#50b3ff"
                     /modified_by="Andrey"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Cole1_no_promoter"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Cole1 no
                     promoter (Cole1_no_promoter)"
                     /label="Cole1 no promoter"
     rep_origin      complement(2535..3152)
                     /direction="RIGHT"
                     /note="color: #ffff00; high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication"
                     /X-Transferred_From="ori"
                     /X-Transferred_Similarity="99.83%"
                     /modified_by="Andrey"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pUC High Copy Cole1 ori"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pUC high
                     copy ori (<a href=""urn:local:Andrey:5z-8qadjwf"">pUC High
                     Copy Cole1 ori</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/-29;
                     ori (<a
                     href=""urn:local:andrey:v-e669fd0"">georgs_sample_05</a>)"
                     /label="pUC high copy ori"
     primer_bind     complement(2624..2642)
                     /Sequence="TAGTCCTGTCGGGTTTCGC"
                     /Hairpin_Tm="39.1"
                     /Tm="59.4"
                     /Self_Dimer_Tm="None"
                     /%GC="57.9"
                     /created_by="primer3"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ASS_Cole1.FOR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Cole1.FOR (ASS_Cole1.FOR)"
                     /label="Cole1.FOR"
     primer_bind     2921..2942
                     /Sequence="TGGTAACAGGATTAGCAGAGCG"
                     /%GC="50.0"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ASS_mc1chk.cole1.REV"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:Andrey:97-96u8sfk"">ASS_mc1chk.cole1.REV<
                     /a>"
                     /label="ASS_mc1chk.cole1.REV"
     promoter        2970..2999
                     /Color="#ffffff"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RNA_I_promoter"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; RNA I
                     promoter (<a
                     href=""urn:local:Andrey:2a-9u4e0yq"">RNA_I_promoter</a>)"
                     /label="RNA I promoter"
     promoter        complement(3124..3152)
                     /Color="#ffffff"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RNAII_promoter"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; RNAII
                     promoter (<a
                     href=""urn:local:Andrey:2g-9u4e0zb"">RNAII_promoter</a>)"
                     /label="RNAII promoter"
ORIGIN      
        1 gaagatcctt tgatcttttc tacggggtct gacgcgcata aataggcgta tcacgaggca
       61 gaatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctgga attcgcggcc
      121 gcttctagag actagtggaa gacatggagt aatacgactc actataggga gatactgggc
      181 ccaagttcac ttaaaaagga gatcaacaat gaaagcaatt ttcgtactga aacatcttaa
      241 tcatgctaag gaggttttct aatgatgaaa acacgtatag tcagctcagt aacaacaaca
      301 ctattgctag gttccatatt aatgaatcct gtcgctaatg ccgcagattc tgatattaat
      361 attaaaaccg gtactacaga tattggaagc aatactacag taaaaacagg tgatttagtc
      421 acttatgata aagaaaatgg catgcacaaa aaagtatttt atagttttat cgatgataaa
      481 aatcataata aaaaactgct agttattaga acgaaaggta ccattgctgg tcaatataga
      541 gtttatagcg aagaaggtgc taacaaaagt ggtttagcct ggccttcagc ctttaaggta
      601 cagttgcaac tacctgataa tgaagtagct caaatatctg attactatcc aagaaattcg
      661 attgatacaa aagagtatat gagtacttta acttatggat tcaacggtaa tgttactggt
      721 gatgatacag gaaaaattgg cggccttatt ggtgcaaatg tttcgattgg tcatacactg
      781 aaatatgttc aacctgattt caaaacaatt ttagagagcc caactgataa aaaagtaggc
      841 tggaaagtga tatttaacaa tatggtgaat caaaattggg gaccatatga tagagattct
      901 tggaacccgg tatatggcaa tcaacttttc atgaaaacta gaaatggctc tatgaaagca
      961 gcagataact tccttgatcc taacaaagca agttctctat tatcttcagg gttttcacca
     1021 gacttcgcta cagttattac tatggataga aaagcatcca aacaacaaac aaatatagat
     1081 gtaatatacg aacgagttcg tgatgactac caattgcact ggacttcaac aaattggaaa
     1141 ggtaccaata ctaaagataa atggatagat cgttcttcag aaagatataa aatcgattgg
     1201 gaaaaagaag aaatgacaaa ttaaaggtct agcataaccc cttggggcct ctaaacgggt
     1261 cttgaggggt tttttggctt atgtcttcta ctagtagcgg ccgctgcagt ccggcaaaaa
     1321 agggcaaggt gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta cttcgcgtta
     1381 tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     1441 cagctcactc aaaggcggta agtcgcgtgt tatacgcccg ttatccatgg gtatggacag
     1501 ttttcccttt gatatgtaac gcacgttgtg tctcaaaatc tctgatgtta cattgcacaa
     1561 gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
     1621 ggtgttatga gccatattca acgggaaacg tcttgctccc gtccgcgctt aaactccaac
     1681 atggacgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg
     1741 acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa
     1801 ggtagcgttg ccaatgatgt tacagatgag atggtccgtc tcaactggct gacggagttt
     1861 atgcctctcc cgaccatcaa gcattttatc cgtactcctg atgatgcgtg gttactcacc
     1921 accgcgattc ctgggaaaac agccttccag gtattagaag aatatcctga ttcaggtgaa
     1981 aatattgttg atgcgctggc cgtgttcctg cgccggttac attcgattcc tgtttgtaat
     2041 tgtcctttta acagcgatcg tgtatttcgt cttgctcagg cgcaatcacg catgaataac
     2101 ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc
     2161 tggaaagaaa tgcacaagct cttgccattc tcaccggatt cagtcgtcac tcatggtgat
     2221 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga
     2281 cgggtcggaa tcgcagaccg ttaccaggac cttgccattc tttggaactg cctcggtgag
     2341 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg
     2401 aataaattgc agtttcattt gatgctcgat gagtttttct aataatacta gcagaaatca
     2461 tccttagcga aagctaagga ttttttttat ctgattaccg cctttgagtg agcgtcgacc
     2521 tagtcctagc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     2581 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     2641 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     2701 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     2761 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     2821 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     2881 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     2941 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     3001 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     3061 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     3121 agcagattac gcgcagaaaa aaaggatctc aa
//
