LOCUS       pFNC-4                  7068 bp ds-DNA     circular     06-SEP-2024
DEFINITION  .
ACCESSION   
VERSION     
KEYWORDS    "Responsible person/group" "Usage" "Status" "Parent plasmids"
            "Cloning sites" "Antibiotic resistance" "Origin of replication"
            "Promoters" "RBS" "Genes" "Tags" "Markers" "Spacers" "gRNAs"
            "Terminators" "DNA fragments" "Protein" "Backbones" "Design entry"
            "LabCollector ID" "Comment" "Box" "Description" "Name"
SOURCE      
  ORGANISM  
FEATURES             Location/Qualifiers
     primer          504..527
                     /label="Primer067_AbR-SwaI-for"
                     /note="sequence: CCAGGGGTCCCCAATAATTACGAT"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          526..551
                     /label="FF293_Sm_resistance_U_fw"
                     /note="sequence: atttaaaTgaaccttgaccgaacgca"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    530..1527
                     /label="from pSEVA4413"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     gene            617..1522
                     /label="aadA gene"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             617..1522
                     /label="aadA CDS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          complement(657..679)
                     /label="NK0517"
                     /note="sequence: GCTCCATAACATCAAACATCGAC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(1506..1531)
                     /label="FF277_SEVA_abEXC_Strep_U_rv"
                     /note="sequence: accgttgTcttatttgccgactacct"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     source          1528..529
                     /label="from pS6213-bxbI"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(1541..1562)
                     /label="Primer068_AbR-BoxI-rev"
                     /note="sequence: GGGTTATGCAGCGGAAAAGGAC"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    1544..1789
                     /label="oriT"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(1576..1605)
                     /label="Primer072_AbR-USER-rev"
                     /note="sequence: AGGATGGATATACCGAAAAAATCGCTATAA"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(1592..1619)
                     /label="Primer143_pSEVA-OriT-rev"
                     /note="sequence: ATATCGTGCGAAAAAGGATGGATATACC"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          1597..1624
                     /label="Primer069_pSEVA-forAbR-USER-for"
                     /note="sequence: ATCCATCCTTTTTCGCACGATATACAGG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          1613..1637
                     /label="Primer146_pSEVA-OriT-USER-for"
                     /note="sequence: ACGATATACAGGATTTTGCCAAAGG"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     CDS             complement(1848..2996)
                     /label="trfA CDS"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     gene            complement(1848..2996)
                     /label="trfA gene"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             complement(1848..2996)
                     /label="Translation 2996-1848"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGSIVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRKTAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTRNKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTFYELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKTSRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLMCGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR*"
     primer          complement(2973..2996)
                     /label="PM_Promotor_rv"
                     /note="sequence: aaggcctaccccttaggCTTTATGCAAGCTTAGGAGGAAAAACATatgaatcggacgtttgaccggaag"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          3378..3405
                     /label="Primer350_pFREEBB_rv"
                     /note="sequence: AGGCCCGCGTTAGCGGGCCGGGAGGGTT"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          3989..4011
                     /label="NK0459"
                     /note="sequence: GATCTCAAGAAGATCCTTTGATC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(4011..4036)
                     /label="FF220_pS62xSac_BB_rv"
                     /note="sequence: agacagcTgggcgcgccgtagaaaag"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          4029..4054
                     /label="FF221_pSxx13_fw"
                     /note="sequence: agctgtcTagggcggcggatttgtcc"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          4034..4051
                     /label=2821
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     terminator      4034..4138
                     /label="T1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          4037..4054
                     /label="Pri_CDV_108_pS1"
                     /note="sequence: AGGGCGGCGGATTTGTCC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     promoter        4149..4213
                     /label="Pem7 promoter"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(4186..4216)
                     /label="FF248_pEM7_U_rv"
                     /note="sequence: attggtTtagttcctcaccttgtcgtattat"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    4234..5736
                     /label="Integrase"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             4234..5736
                     /label="BxbI"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MRALVVIRLSRVTDATTSPERQLESCQQLCAQRGWDVVGVAEDLDVSGAVDPFDRKRRPNLARWLAFEEQPFDVIVAYRVDRLTRSIRHLQQLVHWAEDHKKLVVSATEAHFDTTTPFAAVVIALMGTVAQMELEAIKERNRSAAHFNIRAGKYRGSLPPWGYLPTRVDGEWRLVPDPVQRERILEVYHRVVDNHEPLHLVAHDLNRRGVLSPKDYFAQLQGREPQGREWSATALKRSMISEAMLGYATLNGKTVRDDDGAPLVRAEPILTREQLEALRAELVKTSRAKPAVSTPSLLLRVLFCAVCGEPAYKFAGGGRKHPRYRCRSMGFPKHCGNGTVAMAEWDAFCEEQVLDLLGDAERLEKVWVAGSDSAVELAEVNAELVDLTSLIGSPAYRAGSPQREALDARIAALAARQEELEGLEARPSGWEWRETGQRFGDWWREQDTAAKNTWLRSMNVRLTFDVRGGLTRTIDFGDLQEYEQHLRLGSVVERLHTGMS*"
     primer          4237..4262
                     /label="FF223_Bxb1_fw"
                     /note="sequence: agagcccTggtagtcatccgcctgtc"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(5717..5743)
                     /label="FF224_Bxb1_rv"
                     /note="sequence: agcgttatTacgacatcccggtgtgta"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          5735..5767
                     /label="FF219_pS62xSac_BB_fw"
                     /note="sequence: aataacgcTgatagtgctagtgtagatcgctac"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(5743..5767)
                     /label="RFP_rv"
                     /note="sequence: AGGACTAGTgtagcgatctacactagcactatca"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(5743..5776)
                     /label="Primer362_RFP-fw"
                     /note="sequence: AGGACTAGTGTAGCGATCTACACTAGCACTATCA"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          5768..5798
                     /label="Primer343_pSEVABB_fw"
                     /note="sequence: ACTAGTCCTAGAGTCGACCTGCAGGCATGCA"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          5768..5798
                     /label="Pri_CDV_376_pTn7-trfA.SalI-for"
                     /note="sequence: ACTAGTCCTAGAGTCGACCTGCAGGCATGCA"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          5768..5798
                     /label="Primer376_pTn7-trfA.SalI-for"
                     /note="sequence: ACTAGTCCTAGAGTCGACCTGCAGGCATGCA"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(5776..5802)
                     /label="PPKp-II-R"
                     /note="sequence: AGCTTGCATGCCTGCAGGTCGACTCTA"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          5779..5810
                     /label="Primer468_pSEVA-RFP-U-for"
                     /note="sequence: AGTCGACCTGCAGGCATGCAAGCTTGCGGCCG"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          5794..5814
                     /label="Primer040_pSEVA-U-for"
                     /note="sequence: ATGCAAGCTTGCGGCCGCGTC"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer_bind     complement(5812..5835)
                     /label="F24"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(5812..5835)
                     /label="23-F24"
                     /note="sequence: CGCCAGGGTTTTCCCAGTCACGAC"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          5828..5849
                     /label="Primer073_MCS-SPEI-pSEVA-for"
                     /note="sequence: CCCTGGCGACTAGTCTTGGACT"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(5833..5855)
                     /label="Primer070_pSEVA-forAbR-USER-rev"
                     /note="sequence: AACAGGAGTCCAAGACTAGTCGC"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     terminator      5842..5944
                     /label="T0"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          5847..5875
                     /label="Primer071_AbR-USER-for"
                     /note="sequence: ACTCCTGTTGATAGATCCAGTAATGACCT"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(5895..5915)
                     /label="Primer063_T0-terminator-seq-rev"
                     /note="sequence: CGGCAACCGAGCGTTCTGAAC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(5900..5916)
                     /label="Primer109_pS2"
                     /note="sequence: GCGGCAACCGAGCGTTC"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          complement(5911..5940)
                     /label="Primer489_Prom_Term_U_rev"
                     /note="sequence: ATTCTCACCAATAAAAAACGCCCGGCGGCA"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(5917..5944)
                     /label="Primer516_metH_4044-3316_rev"
                     /note="sequence: CTGGATTCTCACCAATAAAAAACGCCCG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    5952..525
                     /label="Gadget"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer_bind     5953..5972
                     /label="pRS-marker"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             complement(6011..364)
                     /label="SacB"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             complement(6011..364)
                     /label="Translation 364-6011"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISHITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYHIVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEWSGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDGKTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKAYYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDEIERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLKMDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSVVKDSILEQGQLTVNK*"
     primer          complement(6694..6710)
                     /label="oEO_075"
                     /note="sequence: caaccatacgctgagag"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
ORIGIN
        1 aaatcgatgt gtcatccgca tttttaggat ctccggctaa tgcaaagacg atgtggtagc
       61 cgtgatagtt tgcgacagtg ccgtcagcgt tttgtaatgg ccagctgtcc caaacgtcca
      121 ggccttttgc agaagagata tttttaattg tggacgaatc gaactcagga acttgatatt
      181 tttcattttt ttgctgttca gggatttgca gcatatcatg gcgtgtaata tgggaaatgc
      241 cgtatgtttc cttatatggc ttttggttcg tttctttcgc aaacgcttga gttgcgcctc
      301 ctgccagcag tgcggtagta aaggttaata ctgttgcttg ttttgcaaac tttttgatgt
      361 tcatcgttca tgtctccttt tttatgtact gtgttagcgg tctgcttctt ccagccctcc
      421 tgtttgaaga tggcaagtta gttacgcaca ataaaaaaag acctaaaata tgtaaggggt
      481 gacgccaaag tatacacttt gccctttaca cattttaggt cttgcattta aatgaacctt
      541 gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt
      601 ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg
      661 atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa
      721 gttaaacatc atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt
      781 tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc
      841 agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag
      901 gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc
      961 tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat
     1021 tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat
     1081 tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa
     1141 agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt
     1201 tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc
     1261 cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc
     1321 agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc
     1381 ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga
     1441 tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat
     1501 caccaaggta gtcggcaaat aagacaacgg tccgcgcgtt gtccttttcc gctgcataac
     1561 cctgcttcgg ggtcattata gcgatttttt cggtatatcc atcctttttc gcacgatata
     1621 caggattttg ccaaagggtt cgtgtagact ttccttggtg tatccaacgg cgtcagccgg
     1681 gcaggatagg tgaagtaggc ccacccgcga gcgggtgttc cttcttcact gtcccttatt
     1741 cgcacctggc ggtgctcaac gggaatcctg ctctgcgagg ctggccgtag gccggccgcg
     1801 atgcaggtgg ctgctgaacc cccagccgga actgacccca caaggcccta gcgtttgcaa
     1861 tgcaccaggt catcattgac ccaggcgtgt tccaccaggc cgctgcctcg caactcttcg
     1921 caggcttcgc cgacctgctc gcgccacttc ttcacgcggg tggaatccga tccgcacatg
     1981 aggcggaagg tttccagctt gagcgggtac ggctcccggt gcgagctgaa atagtcgaac
     2041 atccgtcggg ccgtcggcga cagcttgcgg tacttctccc atatgaattt cgtgtagtgg
     2101 tcgccagcaa acagcacgac gatttcctcg tcgatcagga cctggcaacg ggacgttttc
     2161 ttgccacggt ccaggacgcg gaagcggtgc agcagcgaca ccgattccag gtgcccaacg
     2221 cggtcggacg tgaagcccat cgccgtcgcc tgtaggcgcg acaggcattc ctcggccttc
     2281 gtgtaatacc ggccattgat cgaccagccc aggtcctggc aaagctcgta gaacgtgaag
     2341 gtgatcggct cgccgatagg ggtgcgcttc gcgtactcca acacctgctg ccacaccagt
     2401 tcgtcatcgt cggcccgcag ctcgacgccg gtgtaggtga tcttcacgtc cttgttgacg
     2461 tggaaaatga ccttgttttg cagcgcctcg cgcgggattt tcttgttgcg cgtggtgaac
     2521 agggcagagc gggccgtgtc gtttggcatc gctcgcatcg tgtccggcca cggcgcaata
     2581 tcgaacaagg aaagctgcat ttccttgatc tgctgcttcg tgtgtttcag caacgcggcc
     2641 tgcttggctt cgctgacctg ttttgccagg tcctcgccgg cggtttttcg cttcttggtc
     2701 gtcatagttc ctcgcgtgtc gatggtcatc gacttcgcca aacctgccgc ctcctgttcg
     2761 agacgacgcg aacgctccac ggcggccgat ggcgcgggca gggcaggggg agccagttgc
     2821 acgctgtcgc gctcgatctt ggccgtagct tgctggacta tcgagccgac ggactggaag
     2881 gtttcgcggg gcgcacgcat gacggtgcgg cttgcgatgg tttcggcatc ctcggcggaa
     2941 aaccccgcgt cgatcagttc ttgcctgtat gccttccggt caaacgtccg attcattcac
     3001 cctccttgcg ggattgcccc ggaattaatt ccccggatcg atccgtcgat cttgatcccc
     3061 tgcgccatca gatccttggc ggcaagaaag ccatccagtt tactttgcag ggcttcccaa
     3121 ccttaccaga gggcgcccca gctggcaatt ccggttcgct tgctgtccat aaaaccgccc
     3181 agtctagcta tcgccatgta agcccactgc aagctacctg ctttctcttt gcgcttgcgt
     3241 tttcccttgt ccagatagcc cagtagctga cattcatccg gggtcagcac cgtttctgcg
     3301 gactggcttt ctacgtggct gccatttttg gggtgaggcc gttcgcggcc gaggggcgca
     3361 gcccctgggg ggatgggagg cccgcgttag cgggccggga gggttcgaga agggggggca
     3421 ccccccttcg gcgtgcgcgg tcacgcgcac agggcgcagc cctggttaaa aacaaggttt
     3481 ataaatattg gtttaaaagc aggttaaaag acaggttagc ggtggccgaa aaacgggcgg
     3541 aaacccttgc aaatgctgga ttttctgcct gtggacagcc cctcaaatgt caataggtgc
     3601 gcccctcatc tgtcagcact ctgcccctca agtgtcaagg atcgcgcccc tcatctgtca
     3661 gtagtcgcgc ccctcaagtg tcaataccgc agggcactta tccccaggct tgtccacatc
     3721 atctgtggga aactcgcgta aaatcaggcg ttttcgccga tttgcgaggc tggccagctc
     3781 cacgtcgccg gccgaaatcg agcctgcccc tcatctgtca acgccgcgcc gggtgagtcg
     3841 gcccctcaag tgtcaacgtc cgcccctcat ctgtcagtga gggccaagtt ttccgcgagg
     3901 tatccacaac gccggcggcc ctacatggct ctgctgtagt gagtgggttg cgctccggca
     3961 gcggtcctga tcccccgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     4021 gcgcgcccag ctgtctaggg cggcggattt gtcctactca ggagagcgtt caccgacaaa
     4081 caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat ttgatgcctt
     4141 taattaatgt gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt
     4201 gaggaactaa acccctaggg gagtcatgaa catatgagag ccctggtagt catccgcctg
     4261 tcccgcgtca ccgatgctac gacttcaccg gagcgtcagc tggagtcttg ccagcagctc
     4321 tgcgcccagc gcggctggga cgtcgtcggg gtagcggagg atctggacgt ctccggggcg
     4381 gtcgatccgt tcgaccggaa gcgcagaccg aacctggccc ggtggctagc gttcgaggag
     4441 caaccgtttg acgtgatcgt ggcgtaccgg gtagatcggt tgacccgatc gatccggcat
     4501 cttcagcagc tggtccactg ggccgaggac cacaagaagc tggtcgtctc cgcgaccgaa
     4561 gcgcacttcg atacgacgac gccgtttgcg gcggtcgtca tcgcgcttat gggaacggtg
     4621 gcgcagatgg aattagaagc gatcaaagag cggaaccgtt cggctgcgca tttcaatatc
     4681 cgcgccggga aataccgagg atccctgccg ccgtggggat acctgcctac gcgcgtggac
     4741 ggggagtggc ggctggtgcc ggaccctgtg cagcgagagc gcatcctcga ggtgtatcac
     4801 cgcgtcgtcg acaaccacga gccgctgcat ctggtggccc acgacctgaa ccggcgtggt
     4861 gtcctgtcgc cgaaggacta cttcgcgcag ctgcaaggcc gcgagccgca gggccgggag
     4921 tggtcggcta ccgcgctgaa gcgatcgatg atctccgagg cgatgctcgg gtacgcgact
     4981 ctgaacggta agaccgtccg agacgacgac ggagccccgc tggtgcgggc tgagccgatc
     5041 ctgacccgtg agcagctgga ggcgctgcgc gccgagctcg tgaagacctc ccgggcgaag
     5101 cccgcggtgt ctaccccgtc gctgctgctg cgggtgttgt tctgcgcggt gtgcggggag
     5161 cccgcgtaca agttcgccgg gggaggacgt aagcacccgc gctaccgctg ccgctcgatg
     5221 gggttcccga agcactgcgg gaacggcacg gtggcgatgg ccgagtggga cgcgttctgc
     5281 gaggagcagg tactggatct gctcggggac gcggagcgtc tggagaaagt ctgggtagcg
     5341 ggctcggact ccgcggtcga actcgcggag gtgaacgcgg agctggtgga cctgacgtcg
     5401 ctgatcggct ccccggccta ccgggcgggc tctccgcagc gagaagcact ggatgcccgt
     5461 attgcggcgc tggccgcgcg gcaagaggag ctggagggcc tggaggctcg cccgtctggc
     5521 tgggagtggc gcgagaccgg gcagcggttc ggggactggt ggcgggagca ggacaccgcg
     5581 gcaaagaaca cctggcttcg gtcgatgaac gttcggctga cgttcgacgt ccgcggcggg
     5641 ctgactcgca cgatcgactt cggggatctt caggagtacg agcagcatct caggctcggc
     5701 agcgtggtcg aacggctaca caccgggatg tcgtaataac gctgatagtg ctagtgtaga
     5761 tcgctacact agtcctagag tcgacctgca ggcatgcaag cttgcggccg cgtcgtgact
     5821 gggaaaaccc tggcgactag tcttggactc ctgttgatag atccagtaat gacctcagaa
     5881 ctccatctgg atttgttcag aacgctcggt tgccgccggg cgttttttat tggtgagaat
     5941 ccaggggtcc cccggcatca gagcagattg tactgagagt gcaccataat cggcattttc
     6001 ttttgcgttt ttatttgtta actgttaatt gtccttgttc aaggatgctg tctttgacaa
     6061 cagatgtttt cttgcctttg atgttcagca ggaagctagg cgcaaacgtt gattgtttgt
     6121 ctgcgtagaa tcctctgttt gtcatatagc ttgtaatcac gacattgttt cctttcgctt
     6181 gaggtacagc gaagtgtgag taagtaaagg ttacatcgtt aggatcaaga tccattttta
     6241 acacaaggcc agttttgttc agcggcttgt atgggccagt taaagaatta gaaacataac
     6301 caagcatgta aatatcgtta gacgtaatgc cgtcaatcgt catttttgat ccgcgggagt
     6361 cagtgaacag ataccatttg ccgttcattt taaagacgtt cgcgcgttca atttcatctg
     6421 ttactgtgtt agatgcaatc agcggtttca tcactttttt cagtgtgtaa tcatcgttta
     6481 gctcaatcat accgagagcg ccgtttgcta actcagccgt gcgtttttta tcgctttgca
     6541 gaagtttttg actttcttga cggaagaatg atgtgctttt gccatagtat gctttgttaa
     6601 ataaagattc ttcgccttgg tagccatctt cagttccagt gtttgcttca aatactaagt
     6661 atttgtggcc tttatcttct acgtagtgag gatctctcag cgtatggttg tcgcctgagc
     6721 tgtagttgcc ttcatcgatg aactgctgta cattttgata cgtttttccg tcaccgtcaa
     6781 agattgattt ataatcctct acaccgttga tgttcaaaga gctgtctgat gctgatacgt
     6841 taacttgtgc agttgtcagt gtttgtttgc cgtaatgttt accggagaaa tcagtgtaga
     6901 ataaacggat ttttccgtca gatgtaaatg tggctgaacc tgaccattct tgtgtttggt
     6961 cttttaggat agaatcattt gcatcgaatt tgtcgctgtc tttaaagacg cggccagcgt
     7021 ttttccagct gtcaatagaa gtttcgccga ctttttgata gaacatgt
//