LOCUS       Exported                3244 bp DNA     circular SYN 16-AUG-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   locusliu
  TITLE     Direct Submission
  JOURNAL   Exported Sep 25, 2024 from SnapGene Viewer 6.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3244
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    234..255
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     promoter        270..300
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                        1: 270 .. 275 / #ffffff / -35
                        2: 276 .. 293 / #ffffff
                        3: 294 .. 300 / #ffffff / -10"
     protein_bind    308..324
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     332..348
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     misc_feature    400..440
                     /label=putative 5'-UTR from hemoglobin alpha
                     /note="color: #666699"
     CDS             488..508
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     misc_feature    512..847
                     /label=VPX
                     /note="color: #ff0000"
     CDS             866..886
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             896..922
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /note="color: #993366"
                     /translation="PAAKRVKLD"
     misc_feature    931..1212
                     /label=AES-mtRNR1 UTR
                     /note="color: #a6acb3"
     promoter        1466..1570
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             1571..2431
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 1571 .. 1639 / #ccffcc / signal sequence
                        2: 1640 .. 2431 / #ccffcc
                     Cleavage site after base 1639"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2602..3190
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
       61 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
      121 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
      181 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
      241 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
      301 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacac cgtcagatcc
      361 gctagagatc cgcggccgct aatacgactc actataagga gaataaacta gtattcttct
      421 ggtccccaca gactcagaag aacccgccac catgaaacgg acagccgacg gaagcgagtt
      481 cgagtcacca aagaagaagc ggaaagtcga catgtcagat cccagggaga gaatcccacc
      541 tggaaacagt ggagaagaga caataggaga agccttcgaa tggctaaaca gaacagtaga
      601 ggagataaac agagaggcag taaaccacct accaagggag ctgattttcc aggtttggca
      661 aaggtcttgg gaatactggc atgatgaaca agggatgtca caaagctatg taaaatacag
      721 atacttgtgt ttaatgcaaa aggctttatt tatgcattgc aagaaaggct gtagatgtct
      781 aggggaagga cacggggcag gaggatggag accaggacct cctcctcctc cccctccagg
      841 actagcatga taagaattcg agtctcccaa gaagaagagg aaagtcggct ctggccctgc
      901 cgctaagaga gtgaagctgg actaatgact cgagctggta ctgcatgcac acaatgctag
      961 ctgccccttt cccgtcctgg gtaccccgag tctcccccga cctcgggtcc caggtatgct
     1021 cccacctcca cctgccccac tcaccacctc tgctagttcc agacacctcc caagcacgca
     1081 gcaatgcagc tcaaaacgct tagcctagcc acacccccac gggaaacagc agtgattaac
     1141 ctttagcaat aaacgaaagt ttaactaagc tatactaacc ccagggttgg tcaatttcgt
     1201 gccagccaca ccctggagct agcaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     1261 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     1321 aaaaaaaaaa aaaaaaaaaa aaaagagacg gtttaaaccc gctgatcagc ctcgactgtg
     1381 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     1441 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     1501 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     1561 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     1621 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     1681 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     1741 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     1801 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     1861 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     1921 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     1981 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     2041 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     2101 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     2161 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     2221 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     2281 gcgtgggtcc cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     2341 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     2401 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     2461 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     2521 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     2581 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     2641 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     2701 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     2761 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     2821 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     2881 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     2941 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     3001 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     3061 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     3121 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     3181 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     3241 tgct
//
