LOCUS       Exported               13118 bp ds-DNA     circular SYN 26-SEP-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pICH47742_p35S-ePEmax3-tEURb7
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 13118)
  AUTHORS   Triple Threat
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Sep 26, 2024 from SnapGene 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13118
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    30..1371
                     /note="p35Slong"
                     /note="color: #cc0000"
     CDS             1495..1515
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /note="SV40 NLS"
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             1516..5616
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /note="Cas9"
                     /note="generates RNA-guided double strand breaks in DNA"
                     /note="color: #993366"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    5674..5841
                     /note="NC"
                     /note="Nucleic acid chaperone"
                     /note="color: #339966"
     CDS             5842..5862
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /note="SV40 NLS"
                     /note="color: #993366"
                     /translation="PKKKRKV"
     misc_feature    5869..5964
                     /note="32aa"
                     /note="color: #ff9900"
     CDS             5972..7512
                     /codon_start=1
                     /note="eRTmax"
                     /note="color: #993366"
                     /translation="LNIEDEYRLHETSKEPDVSLGSTWLSDFPQAWAETGGMGLAVRQA
                     PLIIPLKATSTPVSIKQYPMSQEARLGIKPHIQRLLDQGILVPCQSPWNTPLLPVKKPG
                     TNDYRPVQDLREVNKRVEDIHPTVPNPYNLLSGLPPSHQWYTVLDLKDAFFCLRLHPTS
                     QPLFAFEWRDPEMGISGQLTWTRLPQGFKNSPTLFNEALHRDLADFRIQHPDLILLQYV
                     DDLLLAATSELDCQQGTRALLQTLGNLGYRASAKKAQICQKQVKYLGYLLKEGQRWLTE
                     ARKETVMGQPTPKTPRQLREFLGKAGFCRLFIPGFAEMAAPLYPLTKPGTLFNWGPDQQ
                     KAYQEIKQALLTAPALGLPDLTKPFELFVDEKQGYAKGVLTQKLGPWRRPVAYLSKKLD
                     PVAAGWPPCLRMVAAIAVLTKDAGKLTMGQPLVILAPHAVEALVKQPPDRWLSNARMTH
                     YQALLLDTDRVQFGPVVALNPATLLPLPEEGLQHNCLDILAEAHGTRPDLTDQPL"
     CDS             7558..7578
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /note="SV40 NLS"
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             7588..7614
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /note="c-myc NLS"
                     /note="color: #993366"
                     /translation="PAAKRVKLD"
     misc_feature    7622..8101
                     /note="EU terminator"
                     /note="This feature has 2 segments:
                        1: 7622 .. 8097 / #339966
                        2: 8098 .. 8101 / #339966"
     misc_feature    8102..8312
                     /note="35S terminator"
                     /note="This feature has 2 segments:
                        1: 8102 .. 8201 / #ffff7f
                        2: 8202 .. 8312 / #ffff99"
     misc_feature    8313..8775
                     /note="Rb7 Terminator"
                     /note="color: #993366"
     misc_feature    8807..8934
                     /note="RB short"
                     /note="color: #cc0000"
     misc_feature    8821..8845
                     /note="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
                     /note="color: #ffe4c4"
     CDS             complement(9001..10149)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /note="trfA"
                     /note="color: #993366"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(10485..11073)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(11244..12104)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 11244 .. 12035 / #ccffcc
                        2: 12036 .. 12104 / #ccffcc / signal sequence"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(12105..12209)
                     /gene="bla"
                     /note="AmpR promoter"
                     /note="color: #ffffff; direction: LEFT"
     rep_origin      12296..12927
                     /note="oriV"
                     /note="incP origin of replication"
                     /note="color: #ffff00"
     misc_feature    12949..13096
                     /note="LB"
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    13019..13043
                     /note="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
                     /note="color: #ffe4c4"
ORIGIN
        1 tctgtgaaga caagcaagaa ttcaagcttg gaggaattcc aatcccacaa aaatctgagc
       61 ttaacagcac agttgctcct ctcagagcag aatcgggtat tcaacaccct catatcaact
      121 actacgttgt gtataacggt ccacatgccg gtatatacga tgactggggt tgtacaaagg
      181 cggcaacaaa cggcgttccc ggagttgcac acaagaaatt tgccactatt acagaggcaa
      241 gagcagcagc tgacgcgtac acaacaagtc agcaaacaga caggttgaac ttcatcccca
      301 aaggagaagc tcaactcaag cccaagagct ttgctaaggc cctaacaagc ccaccaaagc
      361 aaaaagccca ctggctcacg ctaggaacca aaaggcccag cagtgatcca gccccaaaag
      421 agatctcctt tgccccggag attacaatgg acgatttcct ctatctttac gatctaggaa
      481 ggaagttcga aggtgaaggt gacgacacta tgttcaccac tgataatgag aaggttagcc
      541 tcttcaattt cagaaagaat gctgacccac agatggttag agaggcctac gcagcaagtc
      601 tcatcaagac gatctacccg agtaacaatc tccaggagat caaatacctt cccaagaagg
      661 ttaaagatgc agtcaaaaga ttcaggacta attgcatcaa gaacacagag aaagacatat
      721 ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt cataaaccaa
      781 ggcaagtaat agagattgga gtctctaaaa aggtagttcc tactgaatct aaggccatgc
      841 atggagtcta agattcaaat cgaggatcta acagaactcg ccgtcaagac tggcgaacag
      901 ttcatacaga gtcttttacg actcaatgac aagaagaaaa tcttcgtcaa catggtggag
      961 cacgacactc tggtctactc caaaaatgtc aaagatacag tctcagaaga tcaaagggct
     1021 attgagactt ttcaacaaag gataatttcg ggaaacctcc tcggattcca ttgcccagct
     1081 atctgtcact tcatcgaaag gacagtagaa aaggaaggtg gctcctacaa atgccatcat
     1141 tgcgataaag gaaaggctat cattcaagat ctctctgccg acagtggtcc caaagatgga
     1201 cccccaccca cgaggagcat cgtggaaaaa gaagaggttc caaccacgtc tacaaagcaa
     1261 gtggattgat gtgacatctc cactgacgta agggatgacg cacaatccca ctatccttcg
     1321 caagaccctt cctctatata aggaagttca tttcatttgg agaggacacg ctcgagtata
     1381 agagctcatt tttacaacaa ttaccaacaa caacaaacaa caaacaacat tacaattaca
     1441 tttacaatta tcgatacaat gaaacggaca gccgacggaa gcgagttcga gtcaccaaag
     1501 aagaagcgga aagtcgacaa gaagtacagc atcggcctgg acatcggcac caactctgtg
     1561 ggctgggccg tgatcaccga cgagtacaag gtgcccagca agaaattcaa ggtgctgggc
     1621 aacaccgacc ggcacagcat caagaagaac ctgatcggag ccctgctgtt cgacagcggc
     1681 gaaacagccg aggccacccg gctgaagaga accgccagaa gaagatacac cagacggaag
     1741 aaccggatct gctatctgca agagatcttc agcaacgaga tggccaaggt ggacgacagc
     1801 ttcttccaca gactggaaga gtccttcctg gtggaagagg ataagaagca cgagcggcac
     1861 cccatcttcg gcaacatcgt ggacgaggtg gcctaccacg agaagtaccc caccatctac
     1921 cacctgagaa agaaactggt ggacagcacc gacaaggccg acctgcggct gatctatctg
     1981 gccctggccc acatgatcaa gttccggggc cacttcctga tcgagggcga cctgaacccc
     2041 gacaacagcg acgtggacaa gctgttcatc cagctggtgc agacctacaa ccagctgttc
     2101 gaggaaaacc ccatcaacgc cagcggcgtg gacgccaagg ccatcctgtc tgccagactg
     2161 agcaagagca gaaagctgga aaatctgatc gcccagctgc ccggcgagaa gaagaatggc
     2221 ctgttcggaa acctgattgc cctgagcctg ggcctgaccc ccaacttcaa gagcaacttc
     2281 gacctggccg aggatgccaa actgcagctg agcaaggaca cctacgacga cgacctggac
     2341 aacctgctgg cccagatcgg cgaccagtac gccgacctgt ttctggccgc caagaacctg
     2401 tccgacgcca tcctgctgag cgacatcctg agagtgaaca ccgagatcac caaggccccc
     2461 ctgagcgcct ctatgatcaa gagatacgac gagcaccacc aggacctgac cctgctgaaa
     2521 gctctcgtgc ggcagcagct gcctgagaag tacaaagaga ttttcttcga ccagagcaag
     2581 aacggctacg ccggctacat tgacggcgga gccagccagg aagagttcta caagttcatc
     2641 aagcccatcc tggaaaagat ggacggcacc gaggaactgc tcgtgaagct gaagagagag
     2701 gacctgctgc ggaagcagcg gaccttcgac aacggcagca tcccccacca gatccacctg
     2761 ggagagctgc acgccattct gcggcggcag gaagattttt acccattcct gaaggacaac
     2821 cgggaaaaga tcgagaagat cctgaccttc cgcatcccct actacgtggg ccctctggcc
     2881 aggggaaaca gcagattcgc ctggatgacc agaaagagcg aggaaaccat caccccctgg
     2941 aacttcgagg aagtggtgga caagggcgct tccgcccaga gcttcatcga gcggatgacc
     3001 aacttcgata agaacctgcc caacgagaag gtgctgccca agcacagcct gctgtacgag
     3061 tacttcaccg tgtataacga gctgaccaaa gtgaaatacg tgaccgaggg aatgagaaag
     3121 cccgccttcc tgagcggcga gcagaaaaag gccatcgtgg acctgctgtt caagaccaac
     3181 cggaaagtga ccgtgaagca gctgaaagag gactacttca agaaaatcga gtgcttcgac
     3241 tccgtggaaa tctccggcgt ggaagatcgg ttcaacgcct ccctgggcac ataccacgat
     3301 ctgctgaaaa ttatcaagga caaggacttc ctggacaatg aggaaaacga ggacattctg
     3361 gaagatatcg tgctgaccct gacactgttt gaggacagag agatgatcga ggaacggctg
     3421 aaaacctatg cccacctgtt cgacgacaaa gtgatgaagc agctgaagcg gcggagatac
     3481 accggctggg gcaggctgag ccggaagctg atcaacggca tccgggacaa gcagtccggc
     3541 aagacaatcc tggatttcct gaagtccgac ggcttcgcca acagaaactt catgcagctg
     3601 atccacgacg acagcctgac ctttaaagag gacatccaga aagcccaggt gtccggccag
     3661 ggcgatagcc tgcacgagca cattgccaat ctggccggca gccccgccat taagaagggc
     3721 atcctgcaga cagtgaaggt ggtggacgag ctcgtgaaag tgatgggccg gcacaagccc
     3781 gagaacatcg tgatcgaaat ggccagagag aaccagacca cccagaaggg acagaagaac
     3841 agccgcgaga gaatgaagcg gatcgaagag ggcatcaaag agctgggcag ccagatcctg
     3901 aaagaacacc ccgtggaaaa cacccagctg cagaacgaga agctgtacct gtactacctg
     3961 cagaatgggc gggatatgta cgtggaccag gaactggaca tcaaccggct gtccgactac
     4021 gatgtggacg ctatcgtgcc tcagagcttt ctgaaggacg actccatcga caacaaggtg
     4081 ctgaccagaa gcgacaagaa ccggggcaag agcgacaacg tgccctccga agaggtcgtg
     4141 aagaagatga agaactactg gcggcagctg ctgaacgcca agctgattac ccagagaaag
     4201 ttcgacaatc tgaccaaggc cgagagaggc ggcctgagcg aactggataa ggccggcttc
     4261 atcaagagac agctggtgga aacccggcag atcacaaagc acgtggcaca gatcctggac
     4321 tcccggatga acactaagta cgacgagaat gacaagctga tccgggaagt gaaagtgatc
     4381 accctgaagt ccaagctggt gtccgatttc cggaaggatt tccagtttta caaagtgcgc
     4441 gagatcaaca actaccacca cgcccacgac gcctacctga acgccgtcgt gggaaccgcc
     4501 ctgatcaaaa agtaccctaa gctggaaagc gagttcgtgt acggcgacta caaggtgtac
     4561 gacgtgcgga agatgatcgc caagagcgag caggaaatcg gcaaggctac cgccaagtac
     4621 ttcttctaca gcaacatcat gaactttttc aagaccgaga ttaccctggc caacggcgag
     4681 atccggaagc ggcctctgat cgagacaaac ggcgaaaccg gggagatcgt gtgggataag
     4741 ggccgggatt ttgccaccgt gcggaaagtg ctgagcatgc cccaagtgaa tatcgtgaaa
     4801 aagaccgagg tgcagacagg cggcttcagc aaagagtcta tcctgcccaa gaggaacagc
     4861 gataagctga tcgccagaaa gaaggactgg gaccctaaga agtacggcgg cttcgacagc
     4921 cccaccgtgg cctattctgt gctggtggtg gccaaagtgg aaaagggcaa gtccaagaaa
     4981 ctgaagagtg tgaaagagct gctggggatc accatcatgg aaagaagcag cttcgagaag
     5041 aatcccatcg actttctgga agccaagggc tacaaagaag tgaaaaagga cctgatcatc
     5101 aagctgccta agtactccct gttcgagctg gaaaacggcc ggaagagaat gctggcctct
     5161 gccggcgaac tgcagaaggg aaacgaactg gccctgccct ccaaatatgt gaacttcctg
     5221 tacctggcca gccactatga gaagctgaag ggctcccccg aggataatga gcagaaacag
     5281 ctgtttgtgg aacagcacaa gcactacctg gacgagatca tcgagcagat cagcgagttc
     5341 tccaagagag tgatcctggc cgacgctaat ctggacaaag tgctgtccgc ctacaacaag
     5401 caccgggata agcccatcag agagcaggcc gagaatatca tccacctgtt taccctgacc
     5461 aatctgggag cccctgccgc cttcaagtac tttgacacca ccatcgaccg gaagaggtac
     5521 accagcacca aagaggtgct ggacgccacc ctgatccacc agagcatcac cggcctgtac
     5581 gagacacgga tcgacctgtc tcagctggga ggtgactccg gcggaagctc tggtggcagc
     5641 aagcggaccg ccgacggctc tgaattcgag agcgccacag tggtgtccgg ccaaaagcag
     5701 gaccgccagg gcggagaacg cagaaggtcc cagctcgata gggatcagtg tgcctactgc
     5761 aaggagaagg gccactgggc caaagactgc ccgaaaaagc cgcgcggccc acgcggccca
     5821 aggccacaaa catccctcct tccaaagaag aagcggaagg tggagctcag cggaggatct
     5881 tccggaggat ctagcggctc cgagacacca ggaacatccg aaagcgctac accagaatct
     5941 agcggaggct cttccggagg atctaggcct accctgaaca ttgaagatga gtatagactg
     6001 catgaaacaa gcaaggaacc cgacgtgtcc ctgggctcca cctggctgtc cgactttccc
     6061 caggcctggg ccgagacagg aggaatgggc ctggccgtgc ggcaggcacc cctgatcatc
     6121 cctctgaagg ccacctctac acccgtgagc atcaagcagt accctatgtc tcaggaggcc
     6181 agactgggca tcaagcctca catccagagg ctgctggacc agggcatcct ggtgccatgc
     6241 cagagcccct ggaacacacc actgctgccc gtgaagaagc caggcaccaa tgactataga
     6301 cccgtgcagg atctgagaga ggtgaacaag agggtggagg atatccaccc caccgtgccc
     6361 aacccttaca atctgctgtc cggcctgccc ccttctcacc agtggtatac agtgctggac
     6421 ctgaaggatg ccttcttttg tctgagactg caccctacca gccagccact gttcgccttt
     6481 gagtggaggg accctgagat gggcatctct ggccagctga cctggacacg cctgcctcag
     6541 ggcttcaaga atagcccaac actgtttaac gaggccctgc accgcgacct ggcagatttc
     6601 cggatccagc acccagatct gatcctgctg cagtacgtgg acgatctgct gctggccgcc
     6661 accagcgagc tggattgcca gcagggaaca cgcgccctgc tgcagaccct gggaaacctg
     6721 ggatataggg catccgccaa gaaggcccag atctgtcaga agcaggtgaa gtacctgggc
     6781 tatctgctga aggagggcca gagatggctg acagaggcca ggaaggagac agtgatgggc
     6841 cagccaacac ccaagacccc aagacagctg agggagttcc tgggcaaagc aggattttgc
     6901 aggctgttca tcccaggatt cgcagagatg gcagcacctc tgtacccact gaccaagccg
     6961 ggcaccctgt ttaattgggg ccctgaccag cagaaggcct atcaggagat caagcaggcc
     7021 ctgctgacag caccagccct gggcctgcca gacctgacca agcctttcga gctgtttgtg
     7081 gatgagaagc agggctacgc caagggcgtg ctgacccaga agctgggacc atggagacgg
     7141 cccgtggcct atctgtccaa gaagctggac ccagtggcag caggatggcc accatgcctg
     7201 aggatggtgg cagcaatcgc cgtgctgaca aaggatgccg gcaagctgac catgggacag
     7261 ccactggtca tcctggcacc acacgcagtg gaggccctgg tgaagcagcc tccagatcgc
     7321 tggctgtcta acgcccggat gacacactac caggccctgc tgctggacac cgatcgcgtg
     7381 cagtttggcc ctgtggtggc cctgaatcca gccaccctgc tgcctctgcc agaggagggc
     7441 ctgcagcaca actgtctgga catcctggca gaggcacacg gaacaaggcc agacctgacc
     7501 gatcagcccc tgtctggcgg ctcaaaaaga accgccgacg gcagcgaatt cgagtctccc
     7561 aagaagaaga ggaaagtcgg ctctggccct gccgctaaga gagtgaagct ggactaagct
     7621 taaagcagaa tgctgagcta aaagaaaggc tttttccatt ttcgagagac aatgagaaaa
     7681 gaagaagaag aagaagaaga agaagaagaa gaaaagagta aataataaag ccccacagga
     7741 ggcgaagttc ttgtagctcc atgttatcta agttattgat attgtttgcc ctatatttta
     7801 tttctgtcat tgtgtatgtt ttgttcagtt tcgatctcct tgcaaaatgc agagattatg
     7861 agatgaataa actaagttat attattatac gtgttaatat tctcctcctc tctctagcta
     7921 gccttttgtt ttctcttttt cttatttgat tttctttaaa tcaatccatt ttaggagagg
     7981 gccagggagt gatccagcaa aacatgaaga ttagaagaaa cttccctctt ttttttcctg
     8041 aaaacaattt aacgtcgaga tttatctctt tttgtaatgg aatcatttct acagttatga
     8101 cgtccgcaaa aatcaccagt ctctctctac aaatctatct ctctctattt ttctccagaa
     8161 taatgtgtga gtagttccca gataagggaa ttagggttct tatagggttt cgctcatgtg
     8221 ttgagcatat aagaaaccct tagtatgtat ttgtatttgt aaaatacttc tatcaataaa
     8281 atttctaatt cctaaaacca aaatccagtg acaccaactc ggtccatttg cacccctaat
     8341 cataatagct ttaatatttc aagatattat taagttaacg ttgtcaatat cctggaaatt
     8401 ttgcaaaatg aatcaagcct atatggctgt aatatgaatt taaaagcagc tcgatgtggt
     8461 ggtaatatgt aatttacttg attctaaaaa aatatcccaa gtattaataa tttctgctag
     8521 gaagaaggtt agctacgatt tacagcaaag ccagaataca aagaaccata aagtgattga
     8581 agctcgaaat atacgaagga acaaatattt ttaaaaaaat acgcaatgac ttggaacaaa
     8641 agaaagtgat atattttttg ttcttaaaca agcatcccct ctaaagaatg gcagttttcc
     8701 tttgcatgta actattatgc tcccttcgtt acaaaaattt tggactacta ttgggaactt
     8761 cttctgaaaa tagtgcgcta ctattgtctt ctgcacgaag tggtttaaac tatcagtgtt
     8821 tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga ataatcggat
     8881 atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat gccaaccaca
     8941 gggttcccca gatcaggcgc tggctgctga acccccagcc ggaactgacc ccacaaggcc
     9001 ctagcgtttg caatgcacca ggtcatcatt gacccaggcg tgttccacca ggccgctgcc
     9061 tcgcaactct tcgcaggctt cgccgacctg ctcgcgccac ttcttcacgc gggtggaatc
     9121 cgatccgcac atgaggcgga aggtttccag cttgagcggg tacggctccc ggtgcgagct
     9181 gaaatagtcg aacatccgtc gggccgtcgg cgacagcttg cggtacttct cccatatgaa
     9241 tttcgtgtag tggtcgccag caaacagcac gacgatttcc tcgtcgatca ggacctggca
     9301 acgggacgtt ttcttgccac ggtccaggac gcggaagcgg tgcagcagcg acaccgattc
     9361 caggtgccca acgcggtcgg acgtgaagcc catcgccgtc gcctgtaggc gcgacaggca
     9421 ttcctcggcc ttcgtgtaat accggccatt gatcgaccag cccaggtcct ggcaaagctc
     9481 gtagaacgtg aaggtgatcg gctcgccgat aggggtgcgc ttcgcgtact ccaacacctg
     9541 ctgccacacc agttcgtcat cgtcggcccg cagctcgacg ccggtgtagg tgatcttcac
     9601 gtccttgttg acgtggaaaa tgaccttgtt ttgcagcgcc tcgcgcggga ttttcttgtt
     9661 gcgcgtggtg aacagggcag agcgggccgt gtcgtttggc atcgctcgca tcgtgtccgg
     9721 ccacggcgca atatcgaaca aggaaagctg catttccttg atctgctgct tcgtgtgttt
     9781 cagcaacgcg gcctgcttgg cctcgctgac ctgttttgcc aggtcctcgc cggcggtttt
     9841 tcgcttcttg gtcgtcatag ttcctcgcgt gtcgatggtc atcgacttcg ccaaacctgc
     9901 cgcctcctgt tcaagacgac gcgaacgctc cacggcggcc gatggcgcgg gcagggcagg
     9961 gggagccagt tgcacgctgt cgcgctcgat cttggccgta gcttgctgga ccatcgagcc
    10021 gacggactgg aaggtttcgc ggggcgcacg catgacggtg cggcttgcga tggtttcggc
    10081 atcctcggcg gaaaaccccg cgtcgatcag ttcttgcctg tatgccttcc ggtcaaacgt
    10141 ccgattcatt caccctcctt gcgggattgc cccgactcac gccggggcaa tgtgccctta
    10201 ttcctgattt gacccgcctg gtgccttggt gtccagataa tccaccttat cggcaatgaa
    10261 gtcggtcccg tagaccgtct ggccgtcctt ctcgtacttg gtattccgaa tcttgccctg
    10321 cacgaatacc agcgacccct tgcccaaata cttgccgtgg gcctcggcct gagagccaaa
    10381 acacttgatg cggaagaagt cggtgcgctc ctgcttgtcg ccggcatcgt tgcgccacat
    10441 ctaggatctg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
    10501 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
    10561 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
    10621 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
    10681 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
    10741 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
    10801 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
    10861 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
    10921 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
    10981 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
    11041 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
    11101 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
    11161 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
    11221 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
    11281 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
    11341 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaacc acgctcaccg
    11401 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    11461 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    11521 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
    11581 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    11641 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    11701 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    11761 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    11821 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    11881 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    11941 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    12001 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    12061 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    12121 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    12181 tagaaaaata aacaaatagg ggttccgcgc acgaattggc cagcgctgcc atttttgggg
    12241 tgaggccgtt cgcggccgag gggcgcagcc cctgggggga tgggaggccc gcgttagcgg
    12301 gccgggaggg ttcgagaagg gggggcaccc cccttcggcg tgcgcggtca cgcgcacagg
    12361 gcgcagccct ggttaaaaac aaggtttata aatattggtt taaaagcagg ttaaaagaca
    12421 ggttagcggt ggccgaaaaa cgggcggaaa cccttgcaaa tgctggattt tctgcctgtg
    12481 gacagcccct caaatgtcaa taggtgcgcc cctcatctgt cagcactctg cccctcaagt
    12541 gtcaaggatc gcgcccctca tctgtcagta gtcgcgcccc tcaagtgtca ataccgcagg
    12601 gcacttatcc ccaggcttgt ccacatcatc tgtgggaaac tcgcgtaaaa tcaggcgttt
    12661 tcgccgattt gcgaggctgg ccagctccac gtcgccggcc gaaatcgagc ctgcccctca
    12721 tctgtcaacg ccgcgccggg tgagtcggcc cctcaagtgt caacgtccgc ccctcatctg
    12781 tcagtgaggg ccaagttttc cgcgaggtat ccacaacgcc ggcggccgcg gtgtctcgca
    12841 cacggcttcg acggcgtttc tggcgcgttt gcagggccat agacggccgc cagcccagcg
    12901 gcgagggcaa ccagcccggt gagcgtcgca aaggagatcc tgatctgact gatgggctgc
    12961 ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg
    13021 gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga
    13081 cgtttttaat gtactggggt ggatgcagtg ggccccac
//
