LOCUS       Exported                4534 bp ds-DNA     circular SYN 26-SEP-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pICH41331_pU6cm-pegR-EURb7
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4534)
  AUTHORS   Triple Threat
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Sep 26, 2024 from SnapGene 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4534
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        110..214
                     /gene="bla"
                     /note="AmpR promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             434..1225
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /note="SmR"
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /note="color: #ccffcc"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1396..1984
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     misc_feature    2236..2670
                     /note="CaMV 35S enhancer"
                     /note="color: #ff9900"
     misc_feature    2671..3140
                     /note="CmYLCV promoter"
                     /note="color: #993300"
     misc_feature    3141..3228
                     /note="U6"
                     /note="color: #ff0000"
     misc_feature    3236..3302
                     /note="tRNA (Gly)"
                     /note="color: #339966"
     misc_feature    3307..3337
                     /note="Spacer cloning site"
                     /note="color: #a6acb3"
     misc_feature    3338..3423
                     /note="altered F+E engineered gRNA scaffold"
                     /note="color: #99ccff"
     misc_feature    3424..3452
                     /note="PBS-RTT cloning site"
                     /note="color: #a6acb3"
     misc_feature    3453..3489
                     /note="tevopreQ1"
                     /note="color: #ffff00"
     misc_feature    3490..3557
                     /note="HDV"
                     /note="color: #000000"
     misc_feature    3570..4055
                     /note="EU"
                     /note="color: #339966"
     misc_feature    4056..4518
                     /note="Rb7"
                     /note="color: #993366"
ORIGIN
        1 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
       61 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      121 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      181 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac
      241 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
      301 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
      361 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac
      421 aaagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
      481 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
      541 cgcagtggat ggcggcctga agccacacag cgatattgat ttgctggtta cggtgaccgt
      601 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
      661 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
      721 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
      781 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
      841 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
      901 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
      961 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     1021 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
     1081 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga
     1141 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccattacg taaaaggcga
     1201 gatcaccaag gtagtcggca aataactgtc agaccaagtt tactcatata tactttagat
     1261 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1321 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1381 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1441 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1501 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     1561 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1621 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1681 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1741 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     1801 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     1861 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1921 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     1981 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     2041 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     2101 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     2161 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatcact
     2221 ctgtggtctc aggagatgga gtcaaagatt caaatagagg acctaacaga actcgccgta
     2281 aagactggcg aacagttcat acagagtctc ttacgactca atgacaagaa gaaaatcttc
     2341 gtcaacatgg tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca
     2401 gaagatcaaa gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga
     2461 ttccattgcc cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc
     2521 tacaaatgcc atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt
     2581 ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga tgttccaacc
     2641 acatcttcaa agcaagtgga ttgatgtgat tggcagacat actgtcccac aaatgaagat
     2701 ggaatctgta aaagaaaacg cgtgaaataa tgcgtctgac aaaggttagg tcggctgcct
     2761 ttaatcaata ccaaagtggt ccctaccacg atggaaaaac tgtgcagtcg gtttggcttt
     2821 ttctgacgaa caaataagat tcgtggccga caggtggggg tccaccatgt gaaggcatct
     2881 tcagactcca ataatggagc aatgacgtaa gggcttacga aataagtaag ggtagtttgg
     2941 gaaatgtcca ctcacccgtc agtctataaa tacttagccc ctccctcatt gttaagggag
     3001 caaaatctca gagagatagt cctagagaga gaaagagagc aagtagccta gaagtagtca
     3061 aggcggcgaa gtattcaggc acgtggccag gaagaagaaa agccaagacg acgaaaacag
     3121 gtaagagcta agcatctaga aagttgaaaa caatcttcaa aagtcccaca tcgcttagat
     3181 aagaaaacga agctgagttt atatacagct agagtcgaag tagtgattga acaaagcacc
     3241 agtggtctag tggtagaata gtaccctgcc acggtacaga cccgggttcg attcccggct
     3301 ggtgcattgt cttcaattcc ttacggatcg aagacaagtt taagagctat gctggaaaca
     3361 gcatagcaag tttaaataag gctagtccgt tatcgacttg aaaaagtcgc accgagtcgg
     3421 tgcttgtctt ctagactagt cgacgaagac aacgcggttc tatctagtta cgcgttaaac
     3481 caactagaag gccggcatgg tcccagcctc ctcgctggcg ccggctgggc aacatgcttc
     3541 ggcatggcga atgggacttt tttttgctta aagcagaatg ctgagctaaa agaaaggctt
     3601 tttccatttt cgagagacaa tgagaaaaga agaagaagaa gaagaagaag aagaagaaga
     3661 agaagaaaag agtaaataat aaagccccac aggaggcgaa gttcttgtag ctccatgtta
     3721 tctaagttat tgatattgtt tgccctatat tttatttctg tcattgtgta tgttttgttc
     3781 agtttcgatc tccttgcaaa atgcagagat tatgagatga ataaactaag ttatattatt
     3841 atacgtgtta atattctcct cctctctcta gctagccttt tgttttctct ttttcttatt
     3901 tgattttctt taaatcaatc cattttagga gagggccagg gagtgatcca gcaaaacatg
     3961 aagattagaa gaaacttccc tctttttttt cctgaaaaca atttaacgtc gagatttatc
     4021 tctttttgta atggaatcat ttctacagtt atgacaccaa ctcggtccat ttgcacccct
     4081 aatcataata gctttaatat ttcaagatat tattaagtta acgttgtcaa tatcctggaa
     4141 attttgcaaa atgaatcaag cctatatggc tgtaatatga atttaaaagc agctcgatgt
     4201 ggtggtaata tgtaatttac ttgattctaa aaaaatatcc caagtattaa taatttctgc
     4261 taggaagaag gttagctacg atttacagca aagccagaat acaaagaacc ataaagtgat
     4321 tgaagctcga aatatacgaa ggaacaaata tttttaaaaa aatacgcaat gacttggaac
     4381 aaaagaaagt gatatatttt ttgttcttaa acaagcatcc cctctaaaga atggcagttt
     4441 tcctttgcat gtaactatta tgctcccttc gttacaaaaa ttttggacta ctattgggaa
     4501 cttcttctga aaatagtgcg cttgagacca cgaa
//
