LOCUS       Exported                8130 bp ds-DNA     circular SYN 24-SEP-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 8130)
  AUTHORS   Adesnik Lab
  TITLE     Direct Submission
  JOURNAL   Exported Oct 1, 2024 from SnapGene 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8130
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    378..509
                     /label=5'ITR
     primer_bind     complement(401..411)
                     /label=Fragment.FOR
     misc_feature    570..1041
                     /label=hSyn
     misc_feature    1059..1064
                     /label=BamHI
     misc_feature    1083..1115
                     /label=lox2722
     misc_feature    1167..1199
                     /label=loxP
     primer_bind     1192..1229
                     /label=Fragment.FOR
     primer_bind     1209..1226
                     /label=320_R
     CDS             complement(1215..1409)
                     /codon_start=1
                     /label=stKv2.1 GCopt
                     /note="stKv2.1 GCopt"
                     /translation="QSQPILNTKEMAPQSKPPEELEMSSMPSPVAPLPARTEGVIDMRS
                     MSSIDSFISCATDFPEATRF"
     CDS             complement(1416..2459)
                     /codon_start=1
                     /label=Chrimson
                     /translation="ELISSATRSLFAAGGINPWPNPYHHEDMGCGGMTPTGECFSTEWW
                     CDPSYGLSDAGYGYCFVEATGGYLVVGVEKKQAWLHSRGTPGEKIGAQVCQWIAFSIAI
                     ALLTFYGFSAWKATCGWEEVYVCCVEVLFVTLEIFKEFSSPATVYLSTGNHAYCLRYFE
                     WLLSCPVILIKLSNLSGLKNDYSKRTMGLIVSCVGMIVFGMAAGLATDWLKWLLYIVSC
                     IYGGYMYFQAAKCYVEANHSVPKGHCRMVVKLMAYAYFASWGSYPILWAVGPEGLLKLS
                     PYANSIGHSICDIIAKEFWTFLAHHLRIKIHEHILIHGDIRKTTKMEIGGEEVEVEEFV
                     EEEDEDTV"
     misc_feature    1698..1715
                     /label=IO-214_S-Crim-3F
                     /note="IO-214_S-Crim-3F"
     gene            complement(1863..1865)
                     /label=M201
     misc_feature    2067..2084
                     /label=IO-213_S-Crim-2F
                     /note="IO-213_S-Crim-2F"
     misc_feature    2487..2507
                     /label=beta-1c
                     /note="beta-1c"
     gene            complement(2502..2813)
                     /label=beta subunit HK-ATPase
                     /note="beta subunit HK-ATPase"
     primer_bind     2803..2839
                     /label=323_R2
     CDS             complement(2820..3527)
                     /codon_start=1
                     /label=mRuby3
                     /translation="VSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTMR
                     IKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGGV
                     VTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTDI
                     ALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAVA
                     KYSNLGGGMDELYK"
     primer_bind     complement(3184..3203)
                     /label=mRub_seq_F
     misc_feature    3324..3332
                     /label=Chromophore
     primer_bind     3327..3347
                     /label=mRuby-R
     primer_bind     3499..3518
                     /label=mRuby_seq_R2
     primer_bind     complement(3504..3553)
                     /label=323_F2
     gene            complement(3537..3596)
                     /label=TS
                     /note="TS"
     primer_bind     3537..3557
                     /label=323_R1
     CDS             complement(3612..4484)
                     /codon_start=1
                     /label=GtACR2
                     /note="GtACR2"
                     /translation="MASQVVYGEWASTHTECYNMSRIDSTFVSLLQLVWAVVSGCQTIF
                     MISRAPKVPWESVYLPFVESITYALASTGNGTLQMRDGRFFPWSRMASWLCTCPIMLGQ
                     ISNMALVKYKSIPLNPIAQAASIIRVVMGITATISPAEYMKWLFFFFGATCLVFEYSVV
                     FTIFQVGLYGFESVGTPLAQKVVVRIKMLRLIFFIAWTMFPIVWLISPTGVCVIHENVS
                     AILYLLADGLCKNTYGVILWSTAWGVLEGKWDPACLPGQEKPEADDPFGLNHEKNAPPN
                     DEVNIRMFGR"
     primer_bind     complement(4470..4507)
                     /label=Fragment.REV
     misc_feature    4498..4529
                     /label=lox2722(1)
     misc_feature    4581..4613
                     /label=loxP(1)
     misc_feature    4627..4632
                     /label=HindIII
     misc_feature    4633..5237
                     /label=WPRE
     primer_bind     complement(4692..4712)
                     /label=WPRE-R
     primer_bind     4692..4712
                     /label=WPRE-F
     misc_feature    5276..5488
                     /label=SV40 polyA
     misc_feature    5559..5689
                     /label=3' ITR
     primer_bind     5657..5667
                     /label=Fragment.FOR
     misc_feature    5871..6188
                     /label=f1 origin
     primer_bind     6136..6154
                     /label=f1 ori
     misc_feature    6452..7311
                     /label=Amp
     primer_bind     6604..6623
                     /label=seq primer 7
     primer_bind     7251..7270
                     /label=seq primer 8
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
       61 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      121 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      181 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      241 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      301 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccagattt
      361 aattaaggcc ttaattaggc tgcgcgctcg ctcgctcact gaggccgccc gggcaaagcc
      421 cgggcgtcgg gcgacctttg gtcgcccggc ctcagtgagc gagcgagcgc gcagagaggg
      481 agtggccaac tccatcacta ggggttcctt gtagttaatg attaacccgc catgctactt
      541 atctacgtag ccatgctcta ggaagatctc tgcagagggc cctgcgtatg agtgcaagtg
      601 ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga ccccacccac
      661 tggacaagca cccaaccccc attccccaaa ttgcgcatcc cctatcagag agggggaggg
      721 gaaacaggat gcggcgaggc gcgtgcgcac tgccagcttc agcaccgcgg acagtgcctt
      781 cgcccccgcc tggcggcgcg cgccaccgcc gcctcagcac tgaaggcgcg ctgacgtcac
      841 tcgccggtcc cccgcaaact ccccttcccg gccaccttgg tcgcgtccgc gccgccgccg
      901 gcccagccgg accgcaccac gcgaggcgcg agataggggg gcacgggcgc gaccatctgc
      961 gctgcggcgc cggcgactca gcgctgcctc agtctgcggt gggcagcgga ggagtcgtgt
     1021 cgtgcctgag agcgcagtcg aattcaagct gatagcaagg atcctctaga gtcgactccg
     1081 gaataacttc gtataggata ctttatacga agttatgcag aatggtagct ggattgtagc
     1141 tgctattagc aatatgaaac ctcttaataa cttcgtatag catacattat acgaagttat
     1201 ggcgcgccag atcagaagcg ggtggcctcg gggaagtcgg tggcgcagct gatgaagctg
     1261 tcgatgctgc tcatgctgcg catgtcgatc acgccctcgg tgcgggcggg caggggggcc
     1321 acggggctgg gcatgctgct catctccagc tcctcggggg gcttgctctg gggggccatc
     1381 tccttggtgt tcaggatggg ctggctctgc tcgagcactg tgtcctcgtc ctcctcctcc
     1441 acgaactctt ccacttccac ctcctcgcct ccgatctcca tcttggtggt cttccggatg
     1501 tcgccgtgga tcaggatgtg ctcgtggatc ttgatcctca ggtggtgggc caggaaggtc
     1561 caaaactcct tggcgatgat gtcgcagatg ctgtggccga tgctgttggc gtaagggctc
     1621 agcttcagca gtccttctgg tcccactgcc cagaggattg ggtagctgcc ccaagaggcg
     1681 aagtaagcgt aggccatcag cttcacgacc atgcggcaat ggcctttagg cacgctgtgg
     1741 ttggcttcca cgtagcactt ggcggcctgg aagtacatgt agccgccgta gatgcaagac
     1801 acgatataca gcagccactt gagccaatcg gtagccagtc ctgcggccat gccgaacacg
     1861 atcattccca cgcaagacac gatcaggccc atggtccgct tgctgtagtc gttcttcagg
     1921 ccgctcaggt tgctcagctt gatcaggatc acggggcaag acagcagcca ctcgaagtag
     1981 cgcaggcaat aggcgtggtt gccggtagac aggtacactg tggcggggct gctgaactcc
     2041 ttgaagatct ccagggtcac gaacagcacc tcgacgcaac agacgtagac ctcctcccaa
     2101 ccgcaagtgg ccttccaggc gctgaagccg tagaatgtca gcagggcgat ggcgatgctg
     2161 aaagcaatcc actggcagac ctgggcgccg atcttttctc ctggtgtgcc tctgctgtgc
     2221 agccaagcct gcttcttctc cactcccacg accaggtagc cgcctgtggc ctccacgaag
     2281 caatatccgt atccggcgtc gctcagtccg taagaagggt cacaccacca ctcggtgctg
     2341 aagcactcgc ctgtaggtgt cattcctcca cagcccatgt cctcgtggtg gtaggggtta
     2401 ggccaagggt tgatgcctcc ggcggcaaac agagatctgg tggcgctgct gatcagctct
     2461 tgtacagctc cctccggacg tccggtgatc tgggcctgtc tagagctgtt ttcagagatg
     2521 ttgtaggaaa tctgcagccc tctttcccca tacacatccg gtctcaaggt tacccccggt
     2581 gactttaact ggtcctggta gtcgggggtg tagggatcaa tggtctgcat cagcacatag
     2641 atgcacaagg caaagagccc agtcatgacc acgtagaaag ctgcatagta caggctgatc
     2701 cacacccacc gggctggggt gcggcccagc atctgcccag tgtccgggtt ccaacagtat
     2761 tgccggaatt cggccatgcg ctggctgcat gacttcttct cctgcagggc tgcaccggtc
     2821 ttgtacagct cgtccatgcc accaccaagg ttgctgtatt tggcaactgc cacttctctt
     2881 tgcactacgt aggtttcatt gtcactctcc tcgatccttt ccaggcggtg atcaacggca
     2941 tggacaccgg gcatcttgat gttcccgacg gtcttttttg acctgtaagt tgtcacgaag
     3001 ttgcagtgca gatggccacc accatcaact ttcagtgcga tgtcagtgta tcctctcaga
     3061 ccaccatctg ctggatacat catctctgta ttaggctccc aacccttggt cttcttctgc
     3121 atcacgggac cattggaggg aaagtttacc cctctgacct tgacgttgta gacgagctcg
     3181 ccatcctcaa ggctggtgtc ctgcgtgacg gtgacgactc caccatcttc gtatctcgta
     3241 actctttccc aagtaaaacc ctcaggaaag gactgtttaa agaaatcagg gatgtcggcc
     3301 gggtacttga taaaggtacg gctgccatac atgaacgacg tggcaagaat gtcaaaggca
     3361 aatggcaggg gtcctccctc gatgactttg atcctcatgg tttgcactcc ctcgtacggt
     3421 ctgccttctc cttcacctgt gcatttgaat tggtggccgt tgaccgaacc ttccatgacc
     3481 accttcatac gcatattttc cttgatcagc tcttcgccct tagacacggg taccgccacg
     3541 ttgatgtcga tctggtccag ggggatgtac tcgccctcgc tggtgatcct gctcttggcc
     3601 gccgtacgtc cccttccaaa cattctgata ttgacttcgt cgttaggagg ggcattcttc
     3661 tcgtgattca ggccaaaagg atcgtcggct tcgggtttct cctgccctgg cagacatgcg
     3721 gggtcccact tcccttccag cactccccag gcagtgctcc acaggatcac tccatatgta
     3781 tttttgcaca ggccgtcagc cagcaggtac agaatggcgc tcacgttctc gtggatcacg
     3841 cagactccgg tgggtgaaat cagccacacg attgggaaca tagtccaagc aataaagaag
     3901 atcaggcgca gcatcttgat cctcaccacc accttctgtg ccagaggtgt cccgacagat
     3961 tcaaagccat acagccccac ttgaaagatt gtgaagacca cactgtactc gaacaccagg
     4021 caggtggccc caaagaaaaa gaacagccac ttcatatact ctgcgggaga aattgtagcg
     4081 gtgattccca tgaccactct aatgatggaa gcggcctgtg caatgggatt cagtgggata
     4141 cttttgtact tcaccagagc catgttagaa atctgtccca gcatgattgg acaggtgcac
     4201 agccaggagg ccattcggct ccaaggaaag aatctgccgt ccctcatctg cagggttccg
     4261 ttgccagtac tggccagtgc ataagtgata gattcgacaa agggcaggta cacggactcc
     4321 caagggacct tgggagccct gctaatcatg aagattgtct ggcagcctga gaccacggcc
     4381 cacaccagct gcagcagtga gacgaaggtg gagtcgattc tggacatatt gtagcactcg
     4441 gtgtgagttg atgcccattc cccatagacg acctgtgatg ccatggttcc gctagcataa
     4501 cttcgtataa agtatcctat acgaagttat ttgccttaac ccagaaatta tcactgttat
     4561 tctttagaat ggtgcaaaga ataacttcgt ataatgtatg ctatacgaag ttatgaattc
     4621 gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     4681 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     4741 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     4801 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     4861 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     4921 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     4981 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     5041 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     5101 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     5161 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     5221 tccccgcatc gataccgtcg acctcgaccc gggcggccgc ttcgagcaga catgataaga
     5281 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     5341 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     5401 aacaacaatt gcattcattt tatgtttcag gttcaggggg agatgtggga ggttttttaa
     5461 agcaagtaaa acctctacaa atgtggtaaa atcgataagg atcttcctag agcatggcta
     5521 cgtagataag tagcatggcg ggttaatcat taactacaag gaacccctag tgatggagtt
     5581 ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg
     5641 acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagcc ttaattaacc
     5701 taattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     5761 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     5821 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
     5881 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     5941 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     6001 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     6061 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     6121 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     6181 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     6241 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     6301 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     6361 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     6421 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     6481 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     6541 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     6601 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     6661 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     6721 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     6781 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     6841 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     6901 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     6961 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     7021 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     7081 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     7141 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     7201 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     7261 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     7321 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     7381 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     7441 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     7501 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     7561 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     7621 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     7681 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     7741 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     7801 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     7861 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     7921 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     7981 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     8041 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     8101 ctggcctttt gctggccttt tgctcacatg
//
