LOCUS       Haimovich_DL_1_p        7481 bp DNA     circular SYN 08-AUG-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7481)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 30, 2024 from SnapGene 7.1.0
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..7481
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(113..217)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: LEFT"
     misc_feature    276..328
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=37.6"
                     /note="/other=misc_feature"
                     /note="color: #a6acb3"
     enhancer        483..862
                     /label=CMV enhancer
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=enhancer"
                     /note="color: #a6acb3; direction: RIGHT"
     promoter        863..1066
                     /label=CMV promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: RIGHT"
     LTR             1062..1095
                     /label=5' LTR (truncated) (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.1"
                     /note="/match_length=18.8"
                     /note="/other=LTR"
                     /note="color: #ffe4c4; direction: RIGHT"
     promoter        1111..1129
                     /label=T7 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             1152..1178
                     /codon_start=1
                     /label=3xHA (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=33.3"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="YPYDVPDYA"
     CDS             1180..1853
                     /codon_start=1
                     /label=mRFP1 (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.9"
                     /note="/match_length=99.4"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="WPPPRTSSRSSCASRCAWRAP*TATSSRSRARARAAPTRAPRPPS
                     *R*PRAAPCPSPGTSCPLSSSTAPRPT*STPPTSPTT*SCPSPRASSGSA**TSRTAAW
                     *P*PRTPPCRTASSSTR*SCAAPTSPPTAP*CRRRPWAGRPPPSGCTPRTAP*RARSR*
                     G*S*RTAATTTPRSRPPTWPRSPCSCPAPTRPTSSWTSPPTMRTTPSWNSTSAPRAATP
                     PA"
     primer_bind     1876..1910
                     /label=AscI MS2 F2
                     /note="color: black; sequence: 
                     agtcGGCGCGCCactgtgctggatatctgcagaattcgggccc; added: 
                     2022-05-14"
     misc_feature    1947..3276
                     /label=24xMS2
                     /note="color: #99cc00"
     misc_RNA        1947..1965
                     /label=MS2 stem loop
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_RNA"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1947..1965
                     /label=MS2SL
                     /note="color: #ff99cc"
     misc_RNA        1986..2004
                     /label=MS2 stem loop
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_RNA"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_RNA        2056..2074
                     /label=MS2 stem loop
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_RNA"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_RNA        2095..2113
                     /label=MS2 stem loop
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_RNA"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_RNA        2165..2183
                     /label=MS2 stem loop
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_RNA"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_RNA        2204..2222
                     /label=MS2 stem loop
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_RNA"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_RNA        2274..2292
                     /label=MS2 stem loop
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_RNA"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_RNA        2313..2331
                     /label=MS2 stem loop
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_RNA"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_RNA        2383..2401
                     /label=MS2 stem loop
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_RNA"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_RNA        2422..2440
                     /label=MS2 stem loop
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_RNA"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_RNA        2492..2510
                     /label=MS2 stem loop
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_RNA"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_RNA        2531..2549
                     /label=MS2 stem loop
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_RNA"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_RNA        2636..2654
                     /label=MS2 stem loop
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_RNA"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_RNA        2675..2693
                     /label=MS2 stem loop
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_RNA"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_RNA        2745..2763
                     /label=MS2 stem loop
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_RNA"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_RNA        2784..2802
                     /label=MS2 stem loop
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_RNA"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_RNA        2854..2872
                     /label=MS2 stem loop
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_RNA"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_RNA        2893..2911
                     /label=MS2 stem loop
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_RNA"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_RNA        2963..2981
                     /label=MS2 stem loop
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_RNA"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_RNA        3002..3020
                     /label=MS2 stem loop
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_RNA"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_RNA        3072..3090
                     /label=MS2 stem loop
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_RNA"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_RNA        3111..3129
                     /label=MS2 stem loop
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_RNA"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_RNA        3181..3199
                     /label=MS2 stem loop
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_RNA"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_RNA        3220..3238
                     /label=MS2 stem loop
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_RNA"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    3220..3238
                     /label=MS2SL
                     /note="color: #ff99cc"
     primer_bind     complement(3272..3289)
                     /label=BamHI MS2 R
                     /note="color: black; sequence: cgagctcggatccgatcg; added: 
                     2022-05-14"
     primer_bind     complement(3293..3327)
                     /label=MS2 Rev
                     /note="color: black; sequence: 
                     gtcgaggctgatcagcggtttaaacttaagcttgg; added: 2022-05-19"
     polyA_signal    3327..3551
                     /label=bGH poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
                     /note="color: #a6acb3; direction: RIGHT"
     promoter        3555..3576
                     /label=SV40 promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=6.1"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      3597..4025
                     /direction=RIGHT
                     /label=f1 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
                     /note="color: #ffff00"
     promoter        4039..4368
                     /label=SV40 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.7"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             4435..5226
                     /codon_start=1
                     /label=neo
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5250..5408
                     /codon_start=1
                     /label=ble (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=98.1"
                     /note="/match_length=42.1"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="MTDQATPNLPSRDFDSTAAFYERLGFGIVFRDAGWMILQRGDLML
                     EFFAHPNL"
     polyA_signal    5403..5524
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
                     /note="color: #a6acb3; direction: RIGHT"
     protein_bind    5597..5613
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
                     /note="color: #31849b"
     promoter        5621..5651
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff"
     protein_bind    5666..5687
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
                     /note="color: #31849b"
     CDS             complement(5700..5792)
                     /codon_start=1
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.6"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="APNTQTASPRALADSLMQLARQVSRLESGQ"
     rep_origin      5975..6562
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.7"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
                     /note="color: #ffff00"
     CDS             complement(join(6733..7481,1..112))
                     /codon_start=1
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #ccffcc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
       61 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
      121 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
      181 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
      241 ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg
      301 ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga
      361 gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa
      421 gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg
      481 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
      541 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
      601 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
      661 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
      721 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
      781 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
      841 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
      901 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
      961 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     1021 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag
     1081 agaacccact gcttactggc ttatcgaaat taatacgact cactataggg agacccaagc
     1141 tggctagcat gtacccatac gatgttccag attacgctgt ggcctcctcc gaggacgtca
     1201 tcaaggagtt catgcgcttc aaggtgcgca tggagggctc cgtgaacggc cacgagttcg
     1261 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
     1321 tgaccaaggg cggccccctg cccttcgcct gggacatcct gtcccctcag ttccagtacg
     1381 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc
     1441 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga
     1501 cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca
     1561 acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctccaccg
     1621 agcggatgta ccccgaggac ggcgccctga agggcgagat caagatgagg ctgaagctga
     1681 aggacggcgg ccactacgac gccgaggtca agaccaccta catggccaag aagcccgtgc
     1741 agctgcccgg cgcctacaag accgacatca agctggacat cacctcccac aatgaggact
     1801 acaccatcgt ggaacagtac gagcgcgccg agggccgcca ctccaccggc gccgggccct
     1861 ctagactcga gcggccgcca ctgtgctgga tatctgcaga attcgggccc tatatatcga
     1921 tcctaaggta cctaattgcc tagaaaacat gaggatcacc catgtctgca ggtcgactcc
     1981 agaaaacatg aggatcaccc atgtctgcag tattcccggg ttcattagat cctaaggtac
     2041 ctaattgcct agaaaacatg aggatcaccc atgtctgcag gtcgactcta gaaaacatga
     2101 ggatcaccca tgtctgcagt attcccgggt tcattagatc ctaaggtacc taattgccta
     2161 gaaaacatga ggatcaccca tgtctgcagg tcgactccag aaaacatgag gatcacccat
     2221 gtctgcagta ttcccgggtt cattagatcc taaggtacct aattgcctag aaaacatgag
     2281 gatcacccat gtctgcaggt cgactctaga aaacatgagg atcacccatg tctgcagtat
     2341 tcccgggttc attagatcct aaggtaccta attgcctaga aaacatgagg atcacccatg
     2401 tctgcaggtc gactctagaa aacatgagga tcacccatgt ctgcagtatt cccgggttca
     2461 ttagatccta aggtacctaa ttgcctagaa aacatgagga tcacccatgt ctgcaggtcg
     2521 actccagaaa acatgaggat cacccatgtc tgcagtattc ccgggttcat tagatctgcg
     2581 cgcgatcgga tcagggccct atatatcgat cctaaggtac cgaattgcct agaaaacatg
     2641 aggatcaccc atgtctgcag gtcgactcca gaaaacatga ggatcaccca tgtctgcagt
     2701 attcccgggt tcattagatc ctaaggtacc taattgccta gaaaacatga ggatcaccca
     2761 tgtctgcagg tcgactctag aaaacatgag gatcacccat gtctgcagta ttcccgggtt
     2821 cattagatcc taaggtacct aattgcctag aaaacatgag gatcacccat gtctgcaggt
     2881 cgactccaga aaacatgagg atcacccatg tctgcagtat tcccgggttc attagatcct
     2941 aaggtaccta attgcctaga aaacatgagg atcacccatg tctgcaggtc gactctagaa
     3001 aacatgagga tcacccatgt ctgcagtatt cccgggttca ttagatccta aggtacctaa
     3061 ttgcctagaa aacatgagga tcacccatgt ctgcaggtcg actctagaaa acatgaggat
     3121 cacccatgtc tgcagtattc ccgggttcat tagatcctaa ggtacctaat tgcctagaaa
     3181 acatgaggat cacccatgtc tgcaggtcga ctccagaaaa catgaggatc acccatgtct
     3241 gcagtattcc cgggttcatt agatctgcgc gcgatcggat ccgagctcgg taccaagctt
     3301 aagtttaaac cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     3361 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     3421 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     3481 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     3541 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
     3601 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     3661 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     3721 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     3781 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     3841 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     3901 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     3961 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     4021 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     4081 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     4141 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     4201 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     4261 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
     4321 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
     4381 gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt
     4441 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     4501 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     4561 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
     4621 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     4681 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     4741 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     4801 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     4861 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     4921 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
     4981 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     5041 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     5101 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     5161 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     5221 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
     5281 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     5341 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     5401 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     5461 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     5521 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     5581 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     5641 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     5701 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     5761 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     5821 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     5881 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     5941 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc cccctgacga
     6001 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     6061 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     6121 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     6181 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     6241 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     6301 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     6361 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     6421 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     6481 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     6541 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     6601 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     6661 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     6721 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     6781 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     6841 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     6901 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     6961 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     7021 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     7081 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     7141 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     7201 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     7261 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     7321 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     7381 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     7441 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc a
//
