LOCUS       pSL17.2_pAAV-hSyn-CLuc- 6918 bp ds-DNA     circular     14-SEP-2023
DEFINITION  .
KEYWORDS    "accession:addgene_114102_252558"
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
COMMENT     Imported from database: AddGene Entry: pAAV-hSyn-iLMO4
            (GLucM23-iChloC-EYFP) URL:
            https://www.addgene.org/browse/sequence/252558/ GENERAL Depositing
            Lab: Ute Hochgeschwender Publication: Park et al J Neurosci Res.
            2017 Sep 1. doi: 10.1002/jnr.24152. Purpose: fusion protein of
            Gaussia luciferase variant M23, improved slowChloC anion conducting
            ChR, and enhanced yellow fluorescent protein for bioluminescent
            optogenetics BACKBONE Backbone size w/o insert (bp): 4593 Total
            vector size (bp): 6931 Vector backbone: pAAV Vector type: Mammalian
            Expression, AAV GROWTH IN BACTERIA Bacterial Resistance(s):
            Ampicillin Copy number: Low Copy Growth Strain(s): NEB Stable
            Growth Temperature: 37°C GENE/INSERT 1 Alt name: GLucM23
            Gene/Insert name: Gaussia luciferase, M23 GENE/INSERT 2 Alt name:
            iChloC Gene/Insert name: improved slowChloC artificial anion
            conducting ChR GENE/INSERT 3 Alt name: EYFP Gene/Insert name:
            Enhanced Yellow Fluorescent Protein
FEATURES             Location/Qualifiers
     repeat_region   5..134
                     /label="AAV2 ITR (alternate)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     promoter        179..626
                     /label="hSyn promoter"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    567..584
                     /label="SLseq1"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    621..639
                     /label="SL132_For"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    621..2337
                     /label="pSL17.2_Fr1"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    640..689
                     /label="SL66_FOR"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    652..670
                     /label="SL14_FOR"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             673..2331
                     /label="CLuc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             673..2331
                     /label="Translation 673-2331"
                     /translation="MKTLILAVALVYCATVHCQDCPYEPDPPNTVPTSCEAKEGECIDSSCGTCTRDILSDGLCENKPGKTCCRMCQYVIECRVEAAGWFRTFYGKRFQFQEPGTYVLGQGTKGGDWKVSITLENLDGTKGAVLTKTRLEVAGDIIDIAQATENPITVNGGADPIIANPYTIGEVTIAVVEMPGFNITVIEFFKLIVIDILGGRSVRIAPDTANKGMISGLCGDLKMMEDTDFTSDPEQLAIQPKINQEFDGCPLYGNPDDVAYCKGLLEPYKDSCRNPINFYYYTISCAFARCMGGDERASHVLLDYRETCAAPETRGTCVLSGHTFYDTFDKARYQFQGPCKEILMAADCFWNTWDVKVSHRNVDSYTEVEKVRIRKQSTVVELIVDGKQILVGGEAVSVPYSSQNTSIYWQDGDILTTAILPEALVVKFNFKQLLVVHIRDPFDGKTCGICGNYNQDFSDDSFDAEGACDLTPNPPGCTEEQKPEAERLCNSLFAGQSDLDQKCNVCHKPDRVERCMYEYCLRGQQGFCDHAWEFKKECYIKHGDTLEVPDECK"
     misc_feature    676..726
                     /label="Signal Peptide"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    2211..2231
                     /label="CLuc-FOR"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(2307..2331)
                     /label="SL67_REV"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(2315..2337)
                     /label="SL132_Rev"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2318..2348
                     /label="SL133_For"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2318..3053
                     /label="pSL17.2_Fr2"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             2332..2337
                     /label="Translation 2332-2337"
                     /translation="GS"
     CDS             2338..2979
                     /label="Translation 2338-2979"
                     /translation="LLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSQEDPDVKFNWYVNGAEVHHAQTKPRETQYNSTYRVVSVLTVTHQDWLNGKEYTCKVSNKALPAPIQKTISKDKGQPREPQVYTLPPSREELTKNQVSLTCLVKGFYPSDIVVEWESSGQPENTYKTTPPVLDSDGSYFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSVSPGK"
     misc_feature    2338..2979
                     /label="NHP IgG1 Fc v2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2338..2979
                     /label="NHP IgG1 Fc (NCBI: JN984927.1)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2463..2482
                     /label="SLseq10"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(2958..3053)
                     /label="SL133_Rev"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             2980..3018
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="GSMASMTGGQQMG"
     CDS             2986..3018
                     /label="T7 tag (gene 10 leader)"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="note: promotes efficient translation in E. coli transl_table: 1 codon_start: 1 translation: MASMTGGQQMG product: leader peptide from bacteriophage T7 gene 10"
     misc_feature    complement(2999..3018)
                     /label="SL126_Rev"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             3019..3021
                     /label="Translation 3019-3021"
                     /translation="*"
     misc_feature    complement(3026..3053)
                     /label="SL10_REV"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    3034..640
                     /label="pSL17.2_Fr3 (pSL11.2 BamHI + EcoRV)"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    3049..3637
                     /label="WPRE"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     complement(3102..3122)
                     /label="WPRE-R"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    complement(3161..3181)
                     /label="SLseq2"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             complement(3520..3531)
                     /label="Factor Xa site"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     polyA_signal    3669..4145
                     /label="hGH poly(A) signal"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     complement(3868..3887)
                     /label="hGH-PA-R"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     repeat_region   4185..4314
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     repeat_region   4185..4325
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     rep_origin      4400..4855
                     /label="f1 ori"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer_bind     complement(4487..4506)
                     /label="F1ori-R"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer_bind     4697..4718
                     /label="F1ori-F"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(4872..4891)
                     /label="pRS-marker"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     4991..5013
                     /label="pGEX 3'"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     complement(5051..5069)
                     /label="pBRforEco"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     promoter        5137..5241
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             5242..6102
                     /label="AmpR"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer_bind     complement(5460..5479)
                     /label="Amp-R"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     rep_origin      6273..6861
                     /label="ori"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     6762..6781
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
ORIGIN
        1 atgtcctgca ggcagctgcg cgctcgctcg ctcactgagg ccgcccgggc gtcgggcgac
       61 ctttggtcgc ccggcctcag tgagcgagcg agcgcgcaga gagggagtgg ccaactccat
      121 cactaggggt tcctgcggcc gcacgcgtgt gtctagactg cagagggccc tgcgtatgag
      181 tgcaagtggg ttttaggacc aggatgaggc ggggtggggg tgcctacctg acgaccgacc
      241 ccgacccact ggacaagcac ccaaccccca ttccccaaat tgcgcatccc ctatcagaga
      301 gggggagggg aaacaggatg cggcgaggcg cgtgcgcact gccagcttca gcaccgcgga
      361 cagtgccttc gcccccgcct ggcggcgcgc gccaccgccg cctcagcact gaaggcgcgc
      421 tgacgtcact cgccggtccc ccgcaaactc cccttcccgg ccaccttggt cgcgtccgcg
      481 ccgccgccgg cccagccgga ccgcaccacg cgaggcgcga gatagggggg cacgggcgcg
      541 accatctgcg ctgcggcgcc ggcgactcag cgctgcctca gtctgcggtg ggcagcggag
      601 gagtcgtgtc gtgcctgaga gcgcagtcga gaaggtaccg gatcctctag agtcgagctc
      661 aagcttgcca ccatgaagac cttaattctt gccgttgcat tagtctactg cgccactgtt
      721 cattgccagg actgtcctta cgaacctgat ccaccaaaca cagttccaac ttcctgtgaa
      781 gctaaagaag gagaatgtat tgatagcagc tgtggcacct gcacgagaga catactatca
      841 gatggactgt gtgaaaataa accaggaaaa acatgttgcc gaatgtgtca gtatgtaatt
      901 gaatgcagag tagaggccgc aggatggttt agaacattct atggaaagag attccagttc
      961 caggaacctg gtacatacgt gttgggtcaa ggaaccaagg gcggcgactg gaaggtgtcc
     1021 atcaccctgg agaacctgga tggaaccaag ggggctgtgc tgaccaagac aagactggaa
     1081 gtggctggag acatcattga catcgctcaa gctactgaga atcccatcac tgtaaacggt
     1141 ggagctgacc ctatcatcgc caacccgtac accatcggcg aggtcaccat cgctgttgtt
     1201 gagatgccag gcttcaacat caccgtcatt gagttcttca aactgatcgt gatcgacatc
     1261 ctcggaggaa gatctgtaag aatcgcccca gacacagcaa acaaaggaat gatctctggc
     1321 ctctgtggag atcttaaaat gatggaagat acagacttca cttcagatcc agaacaactc
     1381 gctattcagc ctaagatcaa ccaggagttt gacggttgtc cactctatgg aaatcctgat
     1441 gacgttgcat actgcaaagg tcttctggag ccgtacaagg acagctgccg caaccccatc
     1501 aacttctact actacaccat ctcctgcgcc ttcgcccgct gtatgggtgg agacgagcga
     1561 gcctcacacg tgctgcttga ctacagggag acgtgcgctg ctcccgaaac tagaggaacc
     1621 tgcgttttgt ctggacatac tttctacgat acatttgaca aagcaagata ccaattccag
     1681 ggtccctgca aggagattct tatggccgcc gactgtttct ggaacacttg ggatgtgaag
     1741 gtttcacaca ggaatgttga ctcttacact gaagtagaga aagtacgaat caggaaacaa
     1801 tcgactgtag tagaactcat tgttgatgga aaacagattc tggttggagg agaagccgtg
     1861 tccgtcccgt acagctctca gaacacttcc atctactggc aagatggtga catactgact
     1921 acagccatcc tacctgaagc tctggtggtc aagttcaact tcaagcaact gctcgtcgta
     1981 catattagag atccattcga tggtaagact tgcggtattt gcggtaacta caaccaggat
     2041 ttcagtgatg attcttttga tgctgaagga gcctgtgatc tgacccccaa cccaccggga
     2101 tgcaccgaag aacagaaacc tgaagctgaa cgactctgca atagtctctt cgccggtcaa
     2161 agtgatcttg atcagaaatg taacgtgtgc cacaagcctg accgtgtcga acgatgcatg
     2221 tacgagtatt gcctgagggg acaacagggt ttctgtgacc acgcatggga gttcaagaaa
     2281 gaatgctaca taaagcatgg agacacccta gaagtaccag atgaatgcaa aGGCtccCTC
     2341 CTGGGGGGAC CGTCAGTCTT CCTCTTCCCC CCAAAACCCA AGGACACCCT CATGATCTCC
     2401 CGGACCCCTG AGGTCACATG CGTGGTGGTA GACGTGAGCC AGGAAGACCC CGATGTCAAG
     2461 TTCAACTGGT ACGTAAACGG CGCGGAGGTG CATCATGCCC AGACGAAGCC ACGGGAGACG
     2521 CAGTACAACA GCACATATCG TGTGGTCAGC GTCCTCACCG TCACGCACCA GGACTGGCTG
     2581 AACGGCAAGG AGTACACGTG CAAGGTCTCC AACAAAGCCC TCCCGGCCCC CATCCAGAAA
     2641 ACCATCTCCA AAGACAAAGG GCAGCCCCGA GAGCCTCAGG TGTACACCCT GCCCCCGTCC
     2701 CGGGAGGAGC TGACCAAGAA CCAGGTCAGC CTGACCTGCC TGGTCAAAGG CTTCTACCCC
     2761 AGCGACATCG TCGTGGAGTG GGAGAGCAGC GGGCAGCCGG AGAACACCTA CAAGACCACG
     2821 CCGCCCGTGC TGGACTCCGA CGGCTCCTAC TTCCTCTACA GCAAGCTCAC CGTGGACAAG
     2881 AGCAGGTGGC AGCAGGGGAA CGTCTTCTCA TGCTCCGTGA TGCATGAGGC TCTGCACAAC
     2941 CACTACACCC AGAAGAGCCT CTCCGTGTCT CCGGGTAAAg gttctatggc tagcatgact
     3001 ggtggacagc aaatgggttg agtttaattc gatatcaagc ttatcgataa tcaacctctg
     3061 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     3121 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     3181 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     3241 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     3301 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     3361 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     3421 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctatgttgcc
     3481 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     3541 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     3601 cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgagc gctgctcgag
     3661 agatctacgg gtggcatccc tgtgacccct ccccagtgcc tctcctggcc ctggaagttg
     3721 ccactccagt gcccaccagc cttgtcctaa taaaattaag ttgcatcatt ttgtctgact
     3781 aggtgtcctt ctataatatt atggggtgga ggggggtggt atggagcaag gggcaagttg
     3841 ggaagacaac ctgtagggcc tgcggggtct attgggaacc aagctggagt gcagtggcac
     3901 aatcttggct cactgcaatc tccgcctcct gggttcaagc gattctcctg cctcagcctc
     3961 ccgagttgtt gggattccag gcatgcatga ccaggctcag ctaatttttg tttttttggt
     4021 agagacgggg tttcaccata ttggccaggc tggtctccaa ctcctaatct caggtgatct
     4081 acccaccttg gcctcccaaa ttgctgggat tacaggcgtg aaccactgct cccttccctg
     4141 tccttctgat tttgtaggta accacgtgcg gaccgagcgg ccgcaggaac ccctagtgat
     4201 ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt
     4261 cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct
     4321 gcaggggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     4381 acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     4441 gttacgcgca gcgtgaccgc tacacttgcc agcgccttag cgcccgctcc tttcgctttc
     4501 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     4561 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt
     4621 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     4681 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ctctatctcg
     4741 ggctattctt ttgatttata agggattttg ccgatttcgg tctattggtt aaaaaatgag
     4801 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattttatgg
     4861 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     4921 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     4981 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     5041 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     5101 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     5161 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     5221 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     5281 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     5341 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     5401 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     5461 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     5521 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     5581 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     5641 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     5701 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     5761 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     5821 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     5881 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     5941 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     6001 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     6061 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     6121 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     6181 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     6241 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     6301 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     6361 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     6421 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     6481 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     6541 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     6601 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     6661 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     6721 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     6781 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     6841 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     6901 tgctggcctt ttgctcac
//