LOCUS       pML374_CLTA-N-term_XTEN 4893 bp ds-DNA     circular     25-SEP-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        complement(675..779)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     893..909
                     /label="M13 fwd"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    940..961
                     /label="Uni_9 F"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    962..982
                     /label="A5.2"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    983..1732
                     /label="CLTA 5' Homology arm"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    1733..1750
                     /label="5' Linker"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1733..2863
                     /label="Payload"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1751..1831
                     /label="3xHA"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1844..2557
                     /label="mEGFP"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2567..2590
                     /label="prescission"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    2606..2845
                     /label="XTEN80"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2846..2863
                     /label="3' Linker"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    2864..3613
                     /label="CLTA 3' Homology arm"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             2864..3613
                     /label="Translation 2864-3613"
                     /translation="AELDPFGAPAGAPGGPALGNGVAGAGEEDPAAAFLAQQESEIAGIENDEAFAILDGGAPGPQPHGEPPGGPGESAGAFGARGLVWKLGPQWVRELLCDSCSLLN*EVREQCKQET*PWPGLSET*AREACSVLSMFECFWARAERESKCRVAGSGAGRSPFNSATFH*AFAVPLVRSLKQPHWRSFSRLISL*V*AEHRTRY*KRRAEKNKNGNIVFERYKQK*QCSSAFKHLRCTECWITRRKEGVGNS"
     misc_feature    2954..2956
                     /label="Mutation A>D"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    3209..3209
                     /label="BsaI mutation"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3394..3394
                     /label="BsaI mutation"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(3614..3633)
                     /label="A3.2"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     source          3634..961
                     /label="source"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    3634..3654
                     /label="Uni_9 R"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     complement(3690..3706)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     rep_origin      complement(3878..4466)
                     /label="ori"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    complement(4583..4607)
                     /label="attB1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             complement(4707..674)
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
       61 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
      121 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
      181 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
      241 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
      301 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
      361 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
      421 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
      481 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
      541 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
      601 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
      661 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
      721 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
      781 acatttcccc gaaaagtgcc agatacctga aacaaaaccc atcgtacggc caaggaagtc
      841 tccaataact gtgatccacc acaagcgcca gggttttccc agtcacgacg ttgtaaaacg
      901 acggccagtc atgcataatc cgcacgcatc tggaataagg aagtgccatt ccgcctgacc
      961 tGGAGCTTAC TGGTGTCACT TCAAAAATAC TTATGGGCGA GAAAAATCGG AGTGGACTGA
     1021 ATCAATTACA GTAAATCTAT GGGCTCTAAG GGAGCAAATA ATCCAGGCTG GCTGAAAGAA
     1081 TTAAATATTT TAAATTTATA AAGCCAGGGA CCCTCAAATC CATAAAAGGA TTCAATCTTC
     1141 TTCTTCtttt tttttAGGat tcaatcttct aataatcccg ggagggggcg caagagcagg
     1201 attcttgttt ctattttacg aatgaggaaa ctgaggttca gaaagcctaa gtggcatgcc
     1261 TCTTCAGTAA TCGGCCAATC GTCGCTGCCC TAACTTCACC TCACCTCCCA GTGCAGCACT
     1321 AGAGTCCCCT CAGGGGTGGC CGTCCCTGGT CAGTCCAGCT CCAGCAGACG GGGTGGGTAG
     1381 GACGCTCCCT ATCAGGCAGC ACTTCCGCCT CCCGGGGCCC GCGCAGCTCA CCTCCCTCAC
     1441 CTCCCGCCCT ACCCCAGTCA CGAGTTGTTT TAGGGGGACC GCCCCTCCAC TTGCTGATTG
     1501 GGTAGCTCCT GAACCATTGT TGTCCTCTGA TTGGTTGTTC CCTTTTCGGC TCTGCAACAC
     1561 CGCCTAGACC GACCGGATAC ACGGGTAGGG CTTCCGCTTT ACCCGTCTCC CTCCTGGCGC
     1621 TTGTCCTCCT CTCCCAGTCG GCACCACAGC GGTGGCTGCC GGGCGTGGTG TCGGTGGGTC
     1681 GGTTGGTTTT TGTCTCACCG TTGGTGTCCG TGCCGTTCAG TTGCCCGCCA TGAGTGGTGG
     1741 TACTTCCGGG TACCCATACG ATGTTCCAGA TTACGCTTAT CCGTATGACG TCCCGGACTA
     1801 TGCATACCCC TACGATGTAC CCGATTACGC CGCTGGAGCT GGCGTGAGCA AGGGCGAGGA
     1861 GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA
     1921 GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGTT
     1981 CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGACCTA
     2041 CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC
     2101 CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA
     2161 CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA
     2221 GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA
     2281 CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG TGAACTTCAA
     2341 GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC
     2401 CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA CCCAGTCCAA
     2461 GCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC
     2521 CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGGGT GGCGGATTGG AAGTTTTGTT
     2581 TCAAGGTCCA GGATCTAACG GCAGCGGAGG GCCGAGCTCT GGCGCACCCC CACCAAGTGG
     2641 AGGGTCACCT GCCGGGTCCC CAACATCTAC TGAAGAAGGC ACCAGCGAAT CCGCAACGCC
     2701 CGAGTCAGGC CCTGGTACCT CCACAGAACC ATCTGAAGGT AGTGCGCCTG GTTCCCCAGC
     2761 TGGAAGCCCT ACTTCCACCG AAGAAGGCAC GTCAACCGAA CCAAGTGAAG GATCTGCCCC
     2821 TGGGACCAGC ACTGAACCAT CTGAGggTGC AgggAGTgga agtGCTGAGC TGGATCCGTT
     2881 CGGCGCCCCT GCCGGCGCCC CTGGCGGTCC CGCGCTGGGG AACGGAGTGG CCGGCGCCGG
     2941 CGAAGAAGAC CCGGCTGCGG CCTTCTTGGC GCAGCAAGAG AGCGAGATTG CGGGCATCGA
     3001 GAACGACGAG GCCTTCGCCA TCCTGGACGG CGGCGCCCCC GGGCCCCAGC CGCACGGCGA
     3061 GCCGCCGGGG GGTCCGGGTG AGAGTGCGGG CGCGTTTGGG GCGAGAGGAC TTGTCTGGAA
     3121 ACTCGGTCCA CAGTGGGTCC GAGAGCTTCT GTGTGACTCG TGCTCCTTGC TGAATTAGGA
     3181 GGTTAGGGAG CAGTGCAAAC AGGAAACGtG ACCCTGGCCC GGTCTTTCAG AAACCTAGGC
     3241 TCGAGAAGCC TGTTCGGTTC TCAGCATGTT TGAGTGCTTC TGGGCGCGGG CGGAGCGAGA
     3301 AAGCAAGTGT AGGGTGGCAG GCTCCGGAGC CGGAAGAAGC CCGTTCAATT CAGCAACTTT
     3361 TCATTAAGCA TTTGCTGTGC CTTTAGTCCG GTCaCTGAAG CAACCGCATT GGCGCAGTTT
     3421 TTCCAGACTT ATAAGCTTAT AAGTCTGAGC CGAGCACAGA ACTCGTTATT AGAAAAGGAG
     3481 GGCGGAAAAA AATAAGAATG GAAATATCGT TTTTGAGAGA TACAAACAAA AGTAGCAATG
     3541 CAGTTCAGCA TTTAAGCACT TAAGGTGTAC AGAGTGTTGG ATTACGAGGA GGAAGGAGGG
     3601 AGTAGGGAAC TCGCGTCAGA AGACCGTCAG TGAaggctag gtggaggctc agtgatgata
     3661 agtctgcgat ggtggatgca tgtgtcatgg tcatagctgt ttcctgtgtg aaattgttat
     3721 ccgctcagag ggcacaatcc tattccgcgc tatccgacaa tctccaagac attaggtgga
     3781 gttcagttcg gcgtatggca tatgtcgctg gaaagaacat gtgagcaaaa ggccagcaaa
     3841 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     3901 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     3961 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     4021 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     4081 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     4141 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     4201 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     4261 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     4321 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     4381 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     4441 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     4501 ctctattcaa caaagccgcc gtcccgtcaa gtcagcgtaa atgggtaggg ggcttcaaat
     4561 cgtcctcgtg ataccaattc ggagcctgct tttttgtaca aacttgttga taatggcaat
     4621 tcaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     4681 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     4741 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     4801 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga gccacgctca
     4861 ccggctccag atttatcagc aataaaccag cca
//