LOCUS       [updated]_pML625_RTN4-N 4393 bp ds-DNA     circular     31-MAR-2025
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name:
            007_pML625_contig.gbk Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     CDS             complement(join(1..251,3784..4393))
                     /label="AmpR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /translation="RARATRSPHTLFSE*LG*VLTSHRKASYGWHDSKRIMQCCHNHE**HCGQLTSDNDRRTEGANRFFAQHGGSCNSP*SLGTGAE*SHTKRRA*HHDACSNGNNVAQTINWRTTYSSFPATINRLDGGG*SCRTTSALGPSGWLVYC**IWSR*AWLSRYHCSTGARW*ALPYRSYLHDGESGNYG*TK*TDR*DRCLTD*ALVNEYSTFPCRPYSLFCGILPSCFCSPRNAGESKRC*RSVGCTSGLHRTGSQQR*DP*EFSPRRTFSNDEHF*SSAMWRGIIPY*R"
     promoter        complement(252..356)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(443..486)
                     /label="lacZα (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="25.3"
                     /fragment="True"
                     /other="CDS"
                     /translation="TGRRFTTS*LGKPW"
     misc_feature    539..559
                     /label="A5.2"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    560..1059
                     /label="5' Homology Arm RTN4"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    1060..1077
                     /label="5' Linker"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1078..1158
                     /label="3x HA"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    1078..2172
                     /label="Payload"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             1105..1131
                     /label="3xHA (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="30.0"
                     /fragment="True"
                     /other="CDS"
                     /translation="YPYDVPDYA"
     misc_feature    1171..1884
                     /label="mEGFP"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             1171..1884
                     /label="mEGFP"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="fpbase"
                     /identity="100.0"
                     /match_length="99.6"
                     /fragment="False"
                     /other="CDS"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     misc_feature    1933..2172
                     /label="XTEN80"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    2173..2190
                     /label="3' Linker"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             2191..2667
                     /label="RTN4 (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="13.3"
                     /fragment="True"
                     /other="CDS"
                     /translation="EDLDQSPLVSSSDSPPRPQPAFKYQFVREPEDEEEEEEEEEEDEDEDLEELEVLERKPAAGLSAAPVPTAPAAGAPLMDFGNDFVPPAPRGPLPAAPPVAPERQPSWDPSPVSSTVPAPSPLSAAAVSPSKLPEDDEPPARPPPPPPASVSPQAEPVWT"
     misc_feature    2191..2690
                     /label="3' Homology Arm RTN4"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    complement(2691..2710)
                     /label="A3.2"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     rep_origin      complement(2955..3543)
                     /label="ori"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     protein_bind    complement(3660..3684)
                     /label="attB1"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
ORIGIN
        1 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
       61 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      121 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      181 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
      241 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
      301 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
      361 tttccccgaa aagtgccaga tacctgaaac aaaacccatc gtacggccaa ggaagtctcc
      421 aataactgtg atccaccaca agcgccaggg ttttcccagt cacgacgttg taaaacgacg
      481 gccagtcatg cataatccgc acgcatctgg aataaggaag tgccattccg cctgacctgg
      541 agcttactgg tgtcacttcc cccaccccaa ctcacggtta cccaaagtgg gggtggagct
      601 cccagctctc agccccgccc attgaacgca atcccagccg gtaagagaga ggaagtgccg
      661 tagtccccac ccactttcat ctccttccct ttctgttggc ccggctggtc gcctatcttc
      721 tcgatcctgt agccgattgg ctctctccct cccgcgccag tccctgccct cccctgggga
      781 gggtgagtca cgccaaactg ggcggagagt ccgctggcct cactcctagc tcatctgggc
      841 ggcggcggca agtggggaca gggcgggtgg cgcatcaccg gcgcggaggc aggaggagca
      901 gtctcattgt tccgggagcc gtcaccacag taggtccctc ggctcagtcg gcccagcccc
      961 tctcagtcct ccccaacccc cacaaccgcc cgcggctctg agacgcggcc ccggcggcgg
     1021 cggcagcagc tgcagcatca tctccaccct ccagccatgt ctggtggtac ttccgggtac
     1081 ccatacgatg ttccagatta cgcttatccg tatgacgtcc cggactatgc atacccctac
     1141 gatgtacccg attacgccgc tggagctggc gtgagcaagg gcgaggagct gttcaccggg
     1201 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     1261 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     1321 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     1381 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     1441 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     1501 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     1561 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     1621 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     1681 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     1741 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct gagcaaagac
     1801 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     1861 ctcggcatgg acgagctgta caagggtggc ggattggaag ttttgtttca aggtccagga
     1921 tctaacggca gcggagggcc gagctctggc gcacccccac caagtggagg gtcacctgcc
     1981 gggtccccaa catctactga agaaggcacc agcgaatccg caacgcccga gtcaggccct
     2041 ggtacctcca cagaaccatc tgaaggtagt gcgcctggtt ccccagctgg aagccctact
     2101 tccaccgaag aaggcacgtc aaccgaacca agtgaaggat ctgcccctgg gaccagcact
     2161 gaaccatctg agggtgcagg gagtggaagt gaagacctgg accagtctcc tctggtctcg
     2221 tcctcggaca gcccaccccg gccgcagccc gcgttcaagt accagttcgt gagggagccc
     2281 gaggacgagg aggaagaaga ggaggaggaa gaggaggacg aggacgaaga cctggaggag
     2341 ctggaggtgc tggagaggaa gcccgccgcc gggctgtccg cggccccagt gcccaccgcc
     2401 cctgccgccg gcgcgcccct gatggacttc ggaaatgact tcgtgccgcc ggcgccccgg
     2461 ggacccctgc cggccgctcc ccccgtcgcc ccggagcggc agccgtcttg ggacccgagc
     2521 ccggtgtcgt cgaccgtgcc cgcgccatcc ccgctgtctg ctgccgcagt ctcgccctcc
     2581 aagctccctg aggacgacga gcctccggcc cggcctcccc ctcctccccc ggccagcgtg
     2641 agcccccagg cagagcccgt gtggaccccg ccagccccgg ctcccgccgc cgtcagaaga
     2701 ccgtcagtga aggctaggtg gaggctcagt gatgataagt ctgcgatggt ggatgcatgt
     2761 gtcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcagagggc acaatcctat
     2821 tccgcgctat ccgacaatct ccaagacatt aggtggagtt cagttcggcg tatggcatat
     2881 gtcgctggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     2941 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     3001 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     3061 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     3121 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     3181 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     3241 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     3301 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     3361 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     3421 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     3481 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     3541 caagaagatc ctttgatctt ttctacgggg tctgacgctc tattcaacaa agccgccgtc
     3601 ccgtcaagtc agcgtaaatg ggtagggggc ttcaaatcgt cctcgtgata ccaattcgga
     3661 gcctgctttt ttgtacaaac ttgttgataa tggcaattca aggatcttca cctagatcct
     3721 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     3781 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     3841 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     3901 ccccagtgct gcaatgatac cgcgagagcc acgctcaccg gctccagatt tatcagcaat
     3961 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     4021 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     4081 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     4141 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     4201 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     4261 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     4321 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     4381 ttgctcttgc ccg
//