LOCUS       pML383_CCDC47-C-term_XT 4893 bp ds-DNA     circular     24-SEP-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        complement(260..364)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     478..494
                     /label="M13 fwd"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    525..546
                     /label="Uni_9 F"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    547..567
                     /label="A5.2"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    568..1317
                     /label="CCDC47 5' Homology arm"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1318..1335
                     /label="5' Linker"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1318..2448
                     /label="Payload"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1336..1575
                     /label="XTEN80"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1585..1608
                     /label="prescission"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             1624..2337
                     /label="mEGFP"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2350..2430
                     /label="3x HA Tag"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2431..2448
                     /label="3' Linker"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    2449..3198
                     /label="CCDC47 3' Homology arm"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    complement(3199..3218)
                     /label="A3.2"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     source          3219..546
                     /label="source"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    3219..3239
                     /label="Uni_9 R"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     complement(3275..3291)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     rep_origin      complement(3463..4051)
                     /label="ori"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    complement(4168..4192)
                     /label="attB1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             complement(4292..259)
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
       61 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
      121 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
      181 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
      241 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
      301 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
      361 cgcgcacatt tccccgaaaa gtgccagata cctgaaacaa aacccatcgt acggccaagg
      421 aagtctccaa taactgtgat ccaccacaag cgccagggtt ttcccagtca cgacgttgta
      481 aaacgacggc cagtcatgca taatccgcac gcatctggaa taaggaagtg ccattccgcc
      541 tgacctGGAG CTTACTGGTG TCACTTCGTG ACCACTAGTG GCCAACAGTG AAGTCATACT
      601 TTGACTTGAC CTCTAAGGCT TCTATCAGTC CACGTAATAA TAACCTGAAC CTTCTTACTC
      661 TTCTATGTAA CTTCCTAATT CCTAAATAAA AATATGATAA ATTTGATAAT TTTTATCACA
      721 TTTGATAAAT ATGATGTCTC AAATAGAGCA TTATGAATTT AAGTATTATG TGCAGGTATG
      781 GTGTCTGTGC TAATAAGAAA TTTGCAATGA ATAACCTGAT GGAACAAGAT TCTTAAAATT
      841 TATTGAACTG AAGTAGAATG TATCTTCTGG CAGTTATACA TTTATGATAT AATGCCATCA
      901 TGAATGGTAT GGTGAACATC AGGCTAAGTG TTCCCTGGGT ACAGAAAAAA ATCAAACCAG
      961 AATAACAAAG GCTGGTTAAT TATAGTAGTT TCTTTCAAAA CTGAGATTTT AGTTTACCTT
     1021 TGCTTCATTT GAAGTTGATT TAATTTTTAT TCACTAATGT AGATATTGGC AATTATTTTT
     1081 ATAACTATCT TCTAGAATGT TATGGTGCAA GCCTAAGTTT TTGAATAATA CTATAGGCAG
     1141 TATTCTAATT TGGAtttttt tttAACATAT GTTTCAAGCT TAATTTGAAT TCTCTCGTCC
     1201 CCACTCCCTT TCTCTATTTA TTACCCCTTC TTTCTCTAGG AGGCTGCATT GAGGCGTGAG
     1261 CAAAAGAAGT TGgaaaagaa gcaaatgaaa atgaaacaaa tcaaagtgaa aGCCATGAGT
     1321 GGTGGTACTT CCGGGGGAGG GCCGAGCTCT GGCGCACCCC CACCAAGTGG AGGGTCACCT
     1381 GCCGGGTCCC CAACATCTAC TGAAGAAGGC ACCAGCGAAT CCGCAACGCC CGAGTCAGGC
     1441 CCTGGTACCT CCACAGAACC ATCTGAAGGT AGTGCGCCTG GTTCCCCAGC TGGAAGCCCT
     1501 ACTTCCACCG AAGAAGGCAC GTCAACCGAA CCAAGTGAAG GATCTGCCCC TGGGACCAGC
     1561 ACTGAACCAT CTGAGGGTGG CGGATTGGAA GTTTTGTTTC AAGGTCCAGG ATCTAACGGC
     1621 AGCgtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     1681 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     1741 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     1801 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     1861 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     1921 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     1981 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     2041 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     2101 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     2161 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     2221 tacctgagca cccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     2281 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagGCT
     2341 GGAGCTGGCT ACCCATACGA TGTTCCAGAT TACGCTTATC CGTATGACGT CCCGGACTAT
     2401 GCATACCCCT ACGATGTACC CGATTACGCC ggTGCAgggA GTggaagtTA AAGCCATCCC
     2461 AGAGATTTGA GTTCTGATGC CACCTGTAAG CTCTGAATTC ACAGGAAACA TGAAAAACGC
     2521 CAGTCCATTT CTCAACCTTA AATTTCAGAC AGTCTTGGGC AACTGAGAAA TCCTTATTTC
     2581 ATCATCTACT CTGTTTGGGG TTTGGGGTTT TACAGAGATT GAAGATACCT GGAAAGGGCT
     2641 CTGTTTCAAG AAtttttttt tCCAGATAAT CAAATTATTT TGATTATTTT ATAAAAGGAA
     2701 TGATCTATGA AATCTGTGTA GGTTTTAAAT ATTTTAAAAA TTATAATACA AATCATCAGT
     2761 GCTTTTAGTA CTTCAGTGTT TAAAGAAATA CCGTGAAATT TATAGGTAGA TAACCAGATT
     2821 GTTGCTTTTT GTTTAAACCA AGCAGTTGAA ATGGCTATAA AGACTGACTC TAAACCAAGA
     2881 TTCTGCAAAT AATGATTGGA ATTGCACAAT AAACATTGCT TGATGTTTTC TTGTATGTCT
     2941 ACATTAAACT TGAGAAAAAG TAAAAATTAG AACACTGTAT GTAGTAATGA AATTTCAGGG
     3001 ACCCAGAACA TAATGTAGTA TATGTTTTTA GGTGGGAGAT GCTGATAACA AAATTAATAG
     3061 GAAGTCTGTA GGCATTAGGA TACTGACATG TACATGGAAA ATTCTAGGGA CAGGAGCATC
     3121 ATTTTTTCCT TACCTGATAC CACGAACCAG TGACAACGTG AATGCTGTAT TTTAAGTGGT
     3181 TGTATGTTTA TTTTCTTGCG TCAGAAGACC GTCAGTGAag gctaggtgga ggctcagtga
     3241 tgataagtct gcgatggtgg atgcatgtgt catggtcata gctgtttcct gtgtgaaatt
     3301 gttatccgct cagagggcac aatcctattc cgcgctatcc gacaatctcc aagacattag
     3361 gtggagttca gttcggcgta tggcatatgt cgctggaaag aacatgtgag caaaaggcca
     3421 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     3481 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     3541 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     3601 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     3661 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     3721 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     3781 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     3841 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     3901 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     3961 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     4021 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     4081 tgacgctcta ttcaacaaag ccgccgtccc gtcaagtcag cgtaaatggg tagggggctt
     4141 caaatcgtcc tcgtgatacc aattcggagc ctgctttttt gtacaaactt gttgataatg
     4201 gcaattcaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     4261 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     4321 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     4381 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagagccac
     4441 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     4501 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     4561 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     4621 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     4681 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     4741 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     4801 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     4861 tctgagaata gtgtatgcgg cgaccgagtt gct
//