LOCUS       pML333_LAMP1-C-term_XTE 3993 bp ds-DNA     circular     24-SEP-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        complement(124..228)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    389..410
                     /label="Uni_9 F"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    411..431
                     /label="A5.2"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    432..731
                     /label="LAMP1 5' Homology Arm"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             720..1914
                     /label="Translation 720-1914"
                     /translation="YQTISGGTSGGGPSSGAPPPSGGSPAGSPTSTEEGTSESATPESGPGTSTEPSEGSAPGSPAGSPTSTEEGTSTEPSEGSAPGTSTEPSEGGGLEVLFQGPGSNGSVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKAGAGYPYDVPDYAYPYDVPDYAYPYDVPDYAGAGSGS*PGARRHSSCRGLCSFL"
     misc_feature    732..749
                     /label="5' Linker"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    732..1862
                     /label="Payload"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    750..989
                     /label="XTEN80"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    999..1022
                     /label="HRV 3C"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             1038..1751
                     /label="mEGFP"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1764..1844
                     /label="3x HA Tag"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1845..1862
                     /label="3' Linker"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     gene            1863..2162
                     /label="LAMP1 3' Homology Arm"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    complement(2163..2182)
                     /label="A3.2"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     backbone        2183..410
                     /label="source"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2183..2203
                     /label="Uni_9 R"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     rep_origin      complement(2427..3015)
                     /label="ori"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    complement(3132..3156)
                     /label="attB1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             complement(3256..123)
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
       61 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
      121 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
      181 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
      241 aaaagtgcca gatacctgaa acaaaaccca tcgtacggcc aaggaagtct ccaataactg
      301 tgatccacca caagcgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtca
      361 tgcataatcc gcacgcatct ggaataagga agtgccattc cgcctgacct GGAGCTTACT
      421 GGTGTCACTT CTCTCTGCAG CGGCTTCCCC AAGTGACAAT TCCAGCTAGA GCTGGAACCT
      481 TCCGCCAGGG TTCCTCTTTG CCTTTTGGCT GATGTGGGGG AGTGGTGGGG AGAGAGGCCT
      541 GTTTGCAGGC CCCTGTGTGA GCAGAGCCCT GACACCATCC GTCTGTCTTG GCAGTGGAGG
      601 AGTGTCTGCT GGACGAGAAC AGCATGCTGA TCCCCATCGC TGTGGGTGGT GCCCTGGCGG
      661 GGCTGGTCCT CATCGTCCTC ATCGCCTACC TCGTCGGCAG GAAGAGGAGT CACGCAGGCT
      721 ACCAGACTAT CAGTGGTGGT ACTTCCGGGG GAGGGCCGAG CTCTGGCGCA CCCCCACCAA
      781 GTGGAGGGTC ACCTGCCGGG TCCCCAACAT CTACTGAAGA AGGCACCAGC GAATCCGCAA
      841 CGCCCGAGTC AGGCCCTGGT ACCTCCACAG AACCATCTGA AGGTAGTGCG CCTGGTTCCC
      901 CAGCTGGAAG CCCTACTTCC ACCGAAGAAG GCACGTCAAC CGAACCAAGT GAAGGATCTG
      961 CCCCTGGGAC CAGCACTGAA CCATCTGAGG GTGGCGGATT GGAAGTTTTG TTTCAAGGTC
     1021 CAGGATCTAA CGGCAGCgtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     1081 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     1141 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     1201 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
     1261 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     1321 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     1381 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     1441 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     1501 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     1561 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     1621 tgcccgacaa ccactacctg agcacccagt ccaagctgag caaagacccc aacgagaagc
     1681 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     1741 agctgtacaa gGCTGGAGCT GGCTACCCAT ACGATGTTCC AGATTACGCT TATCCGTATG
     1801 ACGTCCCGGA CTATGCATAC CCCTACGATG TACCCGATTA CGCCggTGCA gggAGTggaa
     1861 gtTAGCCTGG TGCACGCAGG CACAGCAGCT GCAGGGGCCT CTGTTCCTTT CTCTGGGCTT
     1921 AGGGTCCTGT CGAAGGGGAG GCACACTTTC TGGCAAACGT TTCTCAAATC TGCTTCATCC
     1981 AATGTGAAGT TCATCTTGCA GCATTTACTA TGCACAACAG AGTAACTATC GAAATGACGG
     2041 TGTTAatttt gctaactggg ttaaatattt tgctaactgg ttaaacattA ATATTTACCA
     2101 AAGTAGGATT ttgagggtgg gggtgctctc tctgaggggg tgggggtgcc gctgtctctg
     2161 agCGTCAGAA GACCGTCAGT GAaggctagg tggaggctca gtgatgataa gtctgcgatg
     2221 gtggatgcat gtgtcatggt catagctgtt tcctgtgtga aattgttatc cgctcagagg
     2281 gcacaatcct attccgcgct atccgacaat ctccaagaca ttaggtggag ttcagttcgg
     2341 cgtatggcat atgtcgctgg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     2401 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     2461 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     2521 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     2581 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     2641 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     2701 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     2761 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     2821 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     2881 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     2941 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     3001 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tctattcaac
     3061 aaagccgccg tcccgtcaag tcagcgtaaa tgggtagggg gcttcaaatc gtcctcgtga
     3121 taccaattcg gagcctgctt ttttgtacaa acttgttgat aatggcaatt caaggatctt
     3181 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     3241 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     3301 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     3361 cttaccatct ggccccagtg ctgcaatgat accgcgagag ccacgctcac cggctccaga
     3421 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     3481 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     3541 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     3601 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     3661 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     3721 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     3781 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     3841 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     3901 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     3961 accgctgttg agatccagtt cgatgtaacc cac
//