LOCUS       pML348_ATG101-N-term_XT 4893 bp ds-DNA     circular     24-SEP-2024
DEFINITION  .
KEYWORDS    "#itworked"
FEATURES             Location/Qualifiers
     promoter        complement(252..356)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     470..486
                     /label="M13 fwd"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    517..538
                     /label="Uni_9 F"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    539..559
                     /label="A5.2"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    560..1309
                     /label="ATG101 5' Homology arm"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     exon            1231..1309
                     /label="C12orf44-001 Exon 3"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1310..1327
                     /label="5' Linker"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1310..2440
                     /label="Payload"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1328..1408
                     /label="3xHA"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1421..2134
                     /label="mEGFP"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2144..2167
                     /label="prescission"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    2183..2422
                     /label="XTEN80"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2423..2440
                     /label="3' Linker"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    2441..3190
                     /label="ATG101 3' Homology arm"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    2452..2452
                     /label="Silent mutation"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(3191..3210)
                     /label="A3.2"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     source          3211..538
                     /label="source"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    3211..3231
                     /label="Uni_9 R"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     complement(3267..3283)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     rep_origin      complement(3455..4043)
                     /label="ori"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    complement(4160..4184)
                     /label="attB1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             complement(4284..251)
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
       61 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      121 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      181 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
      241 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
      301 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
      361 tttccccgaa aagtgccaga tacctgaaac aaaacccatc gtacggccaa ggaagtctcc
      421 aataactgtg atccaccaca agcgccaggg ttttcccagt cacgacgttg taaaacgacg
      481 gccagtcatg cataatccgc acgcatctgg aataaggaag tgccattccg cctgacctGG
      541 AGCTTACTGG TGTCACTTCc tgggactaca ggcgcctgcc accaggccca gctaattttt
      601 tatatttttg gtagagatgg ggttttatca tgtttcccag gctggtcttg aacttttgag
      661 ctcaagtgat ttgcctcggc ctcccaagtt gctgggatta atgtgagcca ccacacccgg
      721 ccTAGAGGTA CTTTTTTGAT GCACAGATTT GATCTTGGCT TAGTGATGCA TTGACTCTTC
      781 GCTGCTTTTA GGACAGAGTC CAAATGCCTT AACCTGTTCA TTCAAGGTCC CAGGAAATCA
      841 GACTCCAATC TTTCTGTACT TCTTACTCCC ACCCTGTACT CTCTCCGTGG TGATAAtgtt
      901 tagttgcgtg ctttttcttc atcctgtcag cctgagggca ggggccgtgc ttgcctttga
      961 tgtctagtac ctgacaaatg ttgcacagtg agcatctgct gTAAACTACA TTGAAGGCTG
     1021 TAAAATGGAA TCCCAGCCTG TCATTTGGAC TGATAACTTT GAAGCTCTTG CACTTTCTGA
     1081 TCCTGAGACT TGATGATTCG TTGATAGTGT GTGCTGCCAA AATGCAGAGA AGGGCCAGTG
     1141 GAGGCAGAGG CTGAGCTGAG CCTAGAAGGA AGTGAACAGA TAACACGTGG ACTATTCTTG
     1201 TCAGCATTCT GACCTGGGCT CCTTTTGCAG GGTGGCCCTT GGGTAGGGTG AAGATCCCCT
     1261 GTCTTTATCC TAGTTCCACA CCTTGGTGTG GGTTACTGGG TGCAGGATGA GTGGTGGTAC
     1321 TTCCGGGTAC CCATACGATG TTCCAGATTA CGCTTATCCG TATGACGTCC CGGACTATGC
     1381 ATACCCCTAC GATGTACCCG ATTACGCCGC TGGAGCTGGC GTGAGCAAGG GCGAGGAGCT
     1441 GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT
     1501 CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC TGAAGTTCAT
     1561 CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC TGACCTACGG
     1621 CGTGCAGTGC TTCAGCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC
     1681 CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG GCAACTACAA
     1741 GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG
     1801 CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA ACTACAACAG
     1861 CCACAACGTC TATATCATGG CCGACAAGCA GAAGAACGGC ATCAAGGTGA ACTTCAAGAT
     1921 CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC
     1981 CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC AGTCCAAGCT
     2041 GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC
     2101 CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGGGTGGC GGATTGGAAG TTTTGTTTCA
     2161 AGGTCCAGGA TCTAACGGCA GCGGAGGGCC GAGCTCTGGC GCACCCCCAC CAAGTGGAGG
     2221 GTCACCTGCC GGGTCCCCAA CATCTACTGA AGAAGGCACC AGCGAATCCG CAACGCCCGA
     2281 GTCAGGCCCT GGTACCTCCA CAGAACCATC TGAAGGTAGT GCGCCTGGTT CCCCAGCTGG
     2341 AAGCCCTACT TCCACCGAAG AAGGCACGTC AACCGAACCA AGTGAAGGAT CTGCCCCTGG
     2401 GACCAGCACT GAACCATCTG AGggTGCAgg gAGTggaagt AACTGTCGCT CTGAGGTGCT
     2461 GGAGGTGTCG GTGGAGGGGC GGCAGGTGGA GGAGGCCATG CTGGCTGTGC TGCACACGGT
     2521 GCTTCTGCAC CGCAGCACAG GCAAGTTCCA CTACAAGAAG GAGGGCACCT ACTCCATTGG
     2581 CACCGTGGGC ACCCAGGATG TTGACTGTGA CTTCATCGAC TTCACTTATG TGCGTGTCTC
     2641 TTCTGAGGAA CTGGATCGTG CCCTGCGCaa ggttgttggg gagttcaagg taagggtgtc
     2701 ggggagttca aggtaagggt gtGGCATAGC AGATGGCAGG GGGGCCTCGA GTGCTCCTCC
     2761 ACTCCAGCTT GGTGTTGAAC CAGCCTTCTC TTCCTGAGTG CGTGAATCGG GTTCTTTCAG
     2821 GTTCTGAGAA ACAGATGTGT TGAGAGCTCC TCTTCAGGGG AGGGCGCGCG GGCGCGTGTG
     2881 TGTGTGGTGG GGGAGGGAGA GACTCATAAA TGTGCCTGGG GGAAAAGGGA GAGCAGGAAG
     2941 CTGAGCAGTG TTGGCGGCCA GGTCCCCTGG GGCCATCCTT GCTTCTGTGG TCTTGACCTC
     3001 TCTGGTTACA GCTCTCTCTG CTTCTGCTTC CACTTTTCCT TTCCCACATG AAACTCCCCC
     3061 AGAGGACGGC TTGCTTGGTT CAGGTGGCTG CCATCCAAGA TGGGAGTGTC CCTTTTGGTG
     3121 GTGTGGCATT GTGTGCCTGG GTAGGAGAAA GATGTAGAAG GGACACGGCC GGCTGCTGCT
     3181 GTTGGTCACC CGTCAGAAGA CCGTCAGTGA aggctaggtg gaggctcagt gatgataagt
     3241 ctgcgatggt ggatgcatgt gtcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     3301 ctcagagggc acaatcctat tccgcgctat ccgacaatct ccaagacatt aggtggagtt
     3361 cagttcggcg tatggcatat gtcgctggaa agaacatgtg agcaaaaggc cagcaaaagg
     3421 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     3481 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     3541 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     3601 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     3661 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     3721 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     3781 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     3841 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     3901 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     3961 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     4021 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     4081 tattcaacaa agccgccgtc ccgtcaagtc agcgtaaatg ggtagggggc ttcaaatcgt
     4141 cctcgtgata ccaattcgga gcctgctttt ttgtacaaac ttgttgataa tggcaattca
     4201 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     4261 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     4321 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     4381 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagagcc acgctcaccg
     4441 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     4501 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     4561 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     4621 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     4681 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     4741 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     4801 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     4861 tagtgtatgc ggcgaccgag ttgctcttgc ccg
//