LOCUS       pML318_SEC23A-N-term_XT 3993 bp ds-DNA     circular     24-SEP-2024
DEFINITION  .
KEYWORDS    "#itworked"
FEATURES             Location/Qualifiers
     promoter        complement(105..209)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    392..412
                     /label="A5.2"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    413..712
                     /label="SEC23A 5' Homology Arm"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    496..496
                     /label="BsaI mutation"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    713..730
                     /label="5' Linker"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    713..1843
                     /label="Payload"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    731..811
                     /label="3xHA"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    824..1537
                     /label="mEGFP"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1547..1570
                     /label="prescission"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    1586..1825
                     /label="XTEN80"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1826..1843
                     /label="3' Linker"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1844..2143
                     /label="Sec23A 3' Homology arm"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1984..1984
                     /label="BsaI silent mutation"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(2144..2163)
                     /label="A3.2"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     source          2164..391
                     /label="source"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     rep_origin      complement(2408..2996)
                     /label="ori"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    complement(3113..3137)
                     /label="attB1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             complement(3237..104)
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
       61 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
      121 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
      181 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc agatacctga
      241 aacaaaaccc atcgtacggc caaggaagtc tccaataact gtgatccacc acaagcgcca
      301 gggttttccc agtcacgacg ttgtaaaacg acggccagtc atgcataatc cgcacgcatc
      361 tggaataagg aagtgccatt ccgcctgacc tGGAGCTTAC TGGTGTCACT TCtgggatta
      421 caggcatacg ccaccatgcc tggctaattt tgtattttta gtagagacgg ggtttcttcg
      481 tgttggtcag gctggActcg aactcccgac ctcaggtgat ccgcctgtct cggcctccca
      541 aagtgctggg attaccggcg tgagccacgg ggcccagcTG GTCCTGATTT CTAAGGTGTT
      601 AATATTGCTG AATGAGGGGA GAACATTCAT ATTTTGAACG TTCTTAAGAG GATCCTGGAT
      661 TTTAAAATAT TTTAAAATTT TGATACAGAA ATAAGAATCA AACTCCACAA TGAGTGGTGG
      721 TACTTCCGGG TACCCATACG ATGTTCCAGA TTACGCTTAT CCGTATGACG TCCCGGACTA
      781 TGCATACCCC TACGATGTAC CCGATTACGC CGCTGGAGCT GGCGTGAGCA AGGGCGAGGA
      841 GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA
      901 GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGTT
      961 CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGACCTA
     1021 CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC
     1081 CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA
     1141 CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA
     1201 GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA
     1261 CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG TGAACTTCAA
     1321 GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC
     1381 CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA CCCAGTCCAA
     1441 GCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC
     1501 CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGGGT GGCGGATTGG AAGTTTTGTT
     1561 TCAAGGTCCA GGATCTAACG GCAGCGGAGG GCCGAGCTCT GGCGCACCCC CACCAAGTGG
     1621 AGGGTCACCT GCCGGGTCCC CAACATCTAC TGAAGAAGGC ACCAGCGAAT CCGCAACGCC
     1681 CGAGTCAGGC CCTGGTACCT CCACAGAACC ATCTGAAGGT AGTGCGCCTG GTTCCCCAGC
     1741 TGGAAGCCCT ACTTCCACCG AAGAAGGCAC GTCAACCGAA CCAAGTGAAG GATCTGCCCC
     1801 TGGGACCAGC ACTGAACCAT CTGAGggTGC AgggAGTgga agtACAACCT ATTTaGAATT
     1861 CATTCAACAA AATGAAGAAC GAGATGGAGT CCGATTTAGT TGGAATGTTT GGCCATCAAG
     1921 TCGACTGGAA GCTACAAGAA TGGTTGTTCC TGTGGCAGCC CTGTTTACAC CACTGAAAGA
     1981 GAGgCCTGAC TTACCACCTA TTCAATATGA ACCTGTTCTG TGTAGTAGGA CCACTTGCCG
     2041 TGCAGTTTTG AATCCTTTAT GGTAAGTAAA ATATATAGAA AAACTAATGT GGCAATGTGA
     2101 TTTTAAAATG TCGGGGTTTT TCTGCATATT TTTATAAAAA TCCCGTCAGA AGACCGTCAG
     2161 TGAaggctag gtggaggctc agtgatgata agtctgcgat ggtggatgca tgtgtcatgg
     2221 tcatagctgt ttcctgtgtg aaattgttat ccgctcagag ggcacaatcc tattccgcgc
     2281 tatccgacaa tctccaagac attaggtgga gttcagttcg gcgtatggca tatgtcgctg
     2341 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     2401 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     2461 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     2521 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     2581 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     2641 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     2701 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     2761 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     2821 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     2881 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     2941 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     3001 atcctttgat cttttctacg gggtctgacg ctctattcaa caaagccgcc gtcccgtcaa
     3061 gtcagcgtaa atgggtaggg ggcttcaaat cgtcctcgtg ataccaattc ggagcctgct
     3121 tttttgtaca aacttgttga taatggcaat tcaaggatct tcacctagat ccttttaaat
     3181 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     3241 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     3301 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     3361 gctgcaatga taccgcgaga gccacgctca ccggctccag atttatcagc aataaaccag
     3421 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     3481 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     3541 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     3601 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     3661 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     3721 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     3781 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     3841 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     3901 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     3961 tcgatgtaac ccactcgtgc acccaactga tct
//