LOCUS       pML624_RAB1A-N-term_XTE 4393 bp ds-DNA     circular     29-JAN-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..21
                     /label="Uni_9 R"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     backbone        1..2221
                     /label="source"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     complement(57..73)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     rep_origin      complement(245..833)
                     /label="ori"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    complement(950..974)
                     /label="attB1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             complement(1074..1934)
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     promoter        complement(1935..2039)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     2153..2169
                     /label="M13 fwd"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    2200..2221
                     /label="Uni_9 F"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2222..2242
                     /label="A5.2"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    2243..2742
                     /label="RAB1A 5' Homology Arm"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2743..2760
                     /label="5' Linker"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    2761..2841
                     /label="3X HA"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    2761..3855
                     /label="Payload"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2854..3567
                     /label="mEGFP"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    3616..3855
                     /label="XTEN80"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    3856..3873
                     /label="3' Linker"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    3874..4373
                     /label="RAB1A 3' Homology Arm"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(4374..4393)
                     /label="A3.2"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
ORIGIN
        1 aggctaggtg gaggctcagt gatgataagt ctgcgatggt ggatgcatgt gtcatggtca
       61 tagctgtttc ctgtgtgaaa ttgttatccg ctcagagggc acaatcctat tccgcgctat
      121 ccgacaatct ccaagacatt aggtggagtt cagttcggcg tatggcatat gtcgctggaa
      181 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
      241 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
      301 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
      361 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
      421 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
      481 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
      541 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
      601 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
      661 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
      721 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
      781 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
      841 ctttgatctt ttctacgggg tctgacgctc tattcaacaa agccgccgtc ccgtcaagtc
      901 agcgtaaatg ggtagggggc ttcaaatcgt cctcgtgata ccaattcgga gcctgctttt
      961 ttgtacaaac ttgttgataa tggcaattca aggatcttca cctagatcct tttaaattaa
     1021 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     1081 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     1141 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     1201 gcaatgatac cgcgagagcc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     1261 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     1321 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     1381 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     1441 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     1501 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     1561 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     1621 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     1681 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     1741 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     1801 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     1861 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     1921 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     1981 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     2041 acatttcccc gaaaagtgcc agatacctga aacaaaaccc atcgtacggc caaggaagtc
     2101 tccaataact gtgatccacc acaagcgcca gggttttccc agtcacgacg ttgtaaaacg
     2161 acggccagtc atgcataatc cgcacgcatc tggaataagg aagtgccatt ccgcctgacc
     2221 tGGAGCTTAC TGGTGTCACT TCCCAGGGGG AGTCCGAGCG AAGTCCTAGC CAGCGAGTCA
     2281 GAGGGGAGGG GAGCAGGGAG GGGCCGAGGG TGGGGAGGTG AGGGAGTGGG GAATGGGGCG
     2341 GGCGACAACC CTTCAGGTAC GCATGCCCCA GAGGCGCGGC GCTTGGCGGG AAGCTGAGTC
     2401 CTGGCCTTGC GTCGCACTGT CTGTCCTCAG CTCGCGTAGC CGCGCTCGCG ACTCCCTTTC
     2461 CCGGCATGCC AGGCGGTGCG GCCGCCCTCT GGGCCGTGTA AAGGCCCCTC GGTCTAAGGC
     2521 TTCCCTATTT CCTGGTTCGC CGGCGGCCAT TTTGGGTGGA AGCGATAGCT GAGTGGCGGC
     2581 GGCTGCTGAT TGTGTTCTAG GGGACGGAGT AGGGGAAGAC GTTTGCTCTC CCGGAACAGC
     2641 CTATCTCATT CCTTTCTTTC GATTACCCGT GGCGCGGAGA GTCAGGGCGG CGGCTGCGGC
     2701 AGCAAGGGCG GCGGTGGCGG CGGCGGCAGC TGCAGTGACA TGTCTGGTGG TACTTCCGGG
     2761 TACCCATACG ATGTTCCAGA TTACGCTTAT CCGTATGACG TCCCGGACTA TGCATACCCC
     2821 TACGATGTAC CCGATTACGC CGCTGGAGCT GGCGTGAGCA AGGGCGAGGA GCTGTTCACC
     2881 GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA GTTCAGCGTG
     2941 TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGTT CATCTGCACC
     3001 ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGACCTA CGGCGTGCAG
     3061 TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC
     3121 GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA CAAGACCCGC
     3181 GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA GGGCATCGAC
     3241 TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA CAGCCACAAC
     3301 GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG TGAACTTCAA GATCCGCCAC
     3361 AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC CCCCATCGGC
     3421 GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA CCCAGTCCAA GCTGAGCAAA
     3481 GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCGGGATC
     3541 ACTCTCGGCA TGGACGAGCT GTACAAGGGT GGCGGATTGG AAGTTTTGTT TCAAGGTCCA
     3601 GGATCTAACG GCAGCGGAGG GCCGAGCTCT GGCGCACCCC CACCAAGTGG AGGGTCACCT
     3661 GCCGGGTCCC CAACATCTAC TGAAGAAGGC ACCAGCGAAT CCGCAACGCC CGAGTCAGGC
     3721 CCTGGTACCT CCACAGAACC ATCTGAAGGT AGTGCGCCTG GTTCCCCAGC TGGAAGCCCT
     3781 ACTTCCACCG AAGAAGGCAC GTCAACCGAA CCAAGTGAAG GATCTGCCCC TGGGACCAGC
     3841 ACTGAACCAT CTGAGggTGC AgggAGTgga agtagttctA TGAATCCCGA ATAGTGAGTT
     3901 CAGGAGAGCA CCGGTCGGCT GGGTCCGTGG GCCAGCTTGG GGGATCTTAA AGGGGTCGAG
     3961 GAGGGTTGGG GCAGAAGTCG GGGCATCGGC TGGGGTGAGG CGAGGGTGAT GGGTCAGGAG
     4021 AGGCTGGCGG CCGGGAGTCG GGCCCCATTG TCTGACGCGG AGGGGCGGCC GCGCGGGGGA
     4081 GGGGTCGGGC CGGAGGGGTG AGCCGCCCGG GCCTGGACCG GGTCAGGTTA GAGGGCCTGA
     4141 CTGCGGGGCG GGTGCTGAGG AAGCCTGCCG AGGGGCCTGG GGCGGTGTGA AGGGGTATCT
     4201 TCTCTCGGAG GCAGTGACTT TTGAAGGAGG ACTTGTCTCT AAGGGGAGGG GATGGGGTGG
     4261 GAGAGCCCTT CTAGAGGGCA CTGTCAGACC CTGCGCCCGC ACTCTGCGGA GCTGTCAGGA
     4321 TCTTCGGGGT AGAAACCAGC TTTACTTGTA AATCCTGAGC TTGTTGGCTC TCTCGTCAGA
     4381 AGACCGTCAG TGA
//