LOCUS       pML365_HSP90AA1-N-term_ 3993 bp ds-DNA     circular     24-SEP-2024
DEFINITION  .
KEYWORDS    "#itworked"
FEATURES             Location/Qualifiers
     promoter        complement(260..364)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     478..494
                     /label="M13 fwd"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    525..546
                     /label="Uni_9 F"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    547..567
                     /label="A5.2"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    568..867
                     /label="HSP90AA1 5' Homology arm"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    868..885
                     /label="5' Linker"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    868..1998
                     /label="Payload"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    886..966
                     /label="3xHA"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    979..1692
                     /label="mEGFP"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1702..1725
                     /label="prescission"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    1741..1980
                     /label="XTEN80"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1981..1998
                     /label="3' Linker"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1999..2298
                     /label="HSP90AA1 3' Homology arm"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    complement(2299..2318)
                     /label="A3.2"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     source          2319..546
                     /label="source"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2319..2339
                     /label="Uni_9 R"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     complement(2375..2391)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     rep_origin      complement(2563..3151)
                     /label="ori"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    complement(3268..3292)
                     /label="attB1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             complement(3392..259)
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
       61 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
      121 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
      181 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
      241 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
      301 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
      361 cgcgcacatt tccccgaaaa gtgccagata cctgaaacaa aacccatcgt acggccaagg
      421 aagtctccaa taactgtgat ccaccacaag cgccagggtt ttcccagtca cgacgttgta
      481 aaacgacggc cagtcatgca taatccgcac gcatctggaa taaggaagtg ccattccgcc
      541 tgacctGGAG CTTACTGGTG TCACTTCCCG CCGTGGCGGA GCCGCAGCCC GCGCGGGAGG
      601 GCACCCGGGG TGTTCGTTGG GGACCGCGGC GGGGGACTGG GCCCGGGCCT GCGGCGGCCT
      661 CCCGGAAGCG CGCACACGCT CGTGGTAGTT GCCGCGCTCC GAAATGAGGT CATCCTTTGT
      721 CAGCCGCCCT TCTATTTTCG GTTTACTTAA ACTTCGCAAA TCTCCAATTC GGCTTTAAAA
      781 ATATTTGGAG AACGCGATGG AATTTCGTGT TGCCTCTGTA GACGTCCTGC AAGGTTTTAA
      841 ACCGGCGCGG TGTCGTTCCA GATGCCTAGT GGTGGTACTT CCGGGTACCC ATACGATGTT
      901 CCAGATTACG CTTATCCGTA TGACGTCCCG GACTATGCAT ACCCCTACGA TGTACCCGAT
      961 TACGCCGCTG GAGCTGGCGT GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC
     1021 CTGGTCGAGC TGGACGGCGA CGTAAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGCGAG
     1081 GGCGATGCCA CCTACGGCAA GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC
     1141 GTGCCCTGGC CCACCCTCGT GACCACCCTG ACCTACGGCG TGCAGTGCTT CAGCCGCTAC
     1201 CCCGACCACA TGAAGCAGCA CGACTTCTTC AAGTCCGCCA TGCCCGAAGG CTACGTCCAG
     1261 GAGCGCACCA TCTTCTTCAA GGACGACGGC AACTACAAGA CCCGCGCCGA GGTGAAGTTC
     1321 GAGGGCGACA CCCTGGTGAA CCGCATCGAG CTGAAGGGCA TCGACTTCAA GGAGGACGGC
     1381 AACATCCTGG GGCACAAGCT GGAGTACAAC TACAACAGCC ACAACGTCTA TATCATGGCC
     1441 GACAAGCAGA AGAACGGCAT CAAGGTGAAC TTCAAGATCC GCCACAACAT CGAGGACGGC
     1501 AGCGTGCAGC TCGCCGACCA CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG
     1561 CTGCCCGACA ACCACTACCT GAGCACCCAG TCCAAGCTGA GCAAAGACCC CAACGAGAAG
     1621 CGCGATCACA TGGTCCTGCT GGAGTTCGTG ACCGCCGCCG GGATCACTCT CGGCATGGAC
     1681 GAGCTGTACA AGGGTGGCGG ATTGGAAGTT TTGTTTCAAG GTCCAGGATC TAACGGCAGC
     1741 GGAGGGCCGA GCTCTGGCGC ACCCCCACCA AGTGGAGGGT CACCTGCCGG GTCCCCAACA
     1801 TCTACTGAAG AAGGCACCAG CGAATCCGCA ACGCCCGAGT CAGGCCCTGG TACCTCCACA
     1861 GAACCATCTG AAGGTAGTGC GCCTGGTTCC CCAGCTGGAA GCCCTACTTC CACCGAAGAA
     1921 GGCACGTCAA CCGAACCAAG TGAAGGATCT GCCCCTGGGA CCAGCACTGA ACCATCTGAG
     1981 ggTGCAgggA GTggaagtGA GGAAACCCAG ACCCAAGACC AACCGATGGA GGAGGAGGAG
     2041 GTTGAGACGT TCGCCTTTCA GGCAGAAATT GCCCAGTTGA TGTCATTGAT CATCAATACT
     2101 TTCTACTCGA ACAAAGAGAT CTTTCTGAGA GAGCTCATTT CAAATTCATC AGATGTAAGT
     2161 CACTTATTAA CCCAGAATCG GATTTTGGTT TCAGTGTGAA CTTCTTGGGG GTGCTGTATG
     2221 CTTAAATTAA TATTTTTTGT TAACAGGCAT TGGACAAAAT CCGGTATGAA AGCTTGACAG
     2281 ATCCCAGTAA ATTAGACTCG TCAGAAGACC GTCAGTGAag gctaggtgga ggctcagtga
     2341 tgataagtct gcgatggtgg atgcatgtgt catggtcata gctgtttcct gtgtgaaatt
     2401 gttatccgct cagagggcac aatcctattc cgcgctatcc gacaatctcc aagacattag
     2461 gtggagttca gttcggcgta tggcatatgt cgctggaaag aacatgtgag caaaaggcca
     2521 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     2581 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     2641 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     2701 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     2761 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     2821 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     2881 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     2941 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     3001 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     3061 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     3121 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     3181 tgacgctcta ttcaacaaag ccgccgtccc gtcaagtcag cgtaaatggg tagggggctt
     3241 caaatcgtcc tcgtgatacc aattcggagc ctgctttttt gtacaaactt gttgataatg
     3301 gcaattcaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     3361 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     3421 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     3481 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagagccac
     3541 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     3601 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     3661 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     3721 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     3781 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     3841 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     3901 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     3961 tctgagaata gtgtatgcgg cgaccgagtt gct
//