LOCUS       pML390_YWHAQ-C-term_XTE 4893 bp ds-DNA     circular     25-SEP-2024
DEFINITION  .
KEYWORDS    "#itworked"
FEATURES             Location/Qualifiers
     promoter        complement(122..226)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     340..356
                     /label="M13 fwd"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    387..408
                     /label="Uni_9 F"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    409..429
                     /label="A5.2"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    430..1179
                     /label="YWHAQ 5' Homology arm"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    1180..1197
                     /label="5' Linker"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1180..2310
                     /label="Payload"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1198..1437
                     /label="XTEN80"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1447..1470
                     /label="prescission"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             1486..2199
                     /label="mEGFP"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2139..2292
                     /label="payload"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    2212..2292
                     /label="3x HA Tag"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2293..2310
                     /label="3' Linker"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    2311..3060
                     /label="YWHAQ 3' Homology arm"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    complement(3061..3080)
                     /label="A3.2"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     source          3081..408
                     /label="source"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    3081..3101
                     /label="Uni_9 R"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     complement(3137..3153)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     rep_origin      complement(3325..3913)
                     /label="ori"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    complement(4030..4054)
                     /label="attB1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             complement(4154..121)
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
       61 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
      121 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
      181 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
      241 aagtgccaga tacctgaaac aaaacccatc gtacggccaa ggaagtctcc aataactgtg
      301 atccaccaca agcgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtcatg
      361 cataatccgc acgcatctgg aataaggaag tgccattccg cctgacctGG AGCTTACTGG
      421 TGTCACTTCT GTAATAATAA TGCAGTAGTG CTTTCAAGAA GAGCATTGGG CTGCTGGGAA
      481 TAGAGACTAT CACTTTGCTT ATTCTGAAAG TAGAGTGCCT TTTTAAAAAA Gtttctcttt
      541 tttttttttt tttttgagaa aggatctcac cctgttgcct aggctggagt gcagtgtcat
      601 aatcatggat cactccagcc tcaacctcct ggattcaagc agtcctcctg cctcagcctc
      661 ctgagtagtt aggactgtag gcacacacca ccccgtacct ggccaatttt gtttttgaaa
      721 ttttttgtag acgtgggatc ttgctatgtt gtccaggctg ttgaactcct ggcctcatgc
      781 gatcttctca tcttggcctc cctacgtgct gagattacag gcgtgagcca ctgtgcctgg
      841 ccAAATGCTT TTTAAATAAA AAGGAAGCTA TAAAACCATG CTCCAGTGGt atatgtatgt
      901 atgtatgtat gtcattttgt aagtgcatag tgaaaggtct gcaaggatgt acTTTGGGAA
      961 GGTGATGGTC TTTGGGGTTT TGAGAGAGAA CTTCAGTCTT AGTCAAGATC TCCATTGTTT
     1021 GAGGAATTTA CTACTCTTGT AGTTAATTTT GATATAACAA TACTACTTAG TGTAATTGCC
     1081 TAATCTAGAA ATAGTAACTT AATATGATTC TTCTTTTTCT AGCTTTGGAC ATCAGACAGT
     1141 GCAGGAGAAG AATGTGATGC GGCAGAAGGG GCTGAAAACA GTGGTGGTAC TTCCGGGGGA
     1201 GGGCCGAGCT CTGGCGCACC CCCACCAAGT GGAGGGTCAC CTGCCGGGTC CCCAACATCT
     1261 ACTGAAGAAG GCACCAGCGA ATCCGCAACG CCCGAGTCAG GCCCTGGTAC CTCCACAGAA
     1321 CCATCTGAAG GTAGTGCGCC TGGTTCCCCA GCTGGAAGCC CTACTTCCAC CGAAGAAGGC
     1381 ACGTCAACCG AACCAAGTGA AGGATCTGCC CCTGGGACCA GCACTGAACC ATCTGAGGGT
     1441 GGCGGATTGG AAGTTTTGTT TCAAGGTCCA GGATCTAACG GCAGCgtgag caagggcgag
     1501 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     1561 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     1621 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
     1681 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     1741 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     1801 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     1861 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     1921 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     1981 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     2041 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     2101 aagctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     2161 gccgccggga tcactctcgg catggacgag ctgtacaagG CTGGAGCTGG CTACCCATAC
     2221 GATGTTCCAG ATTACGCTTA TCCGTATGAC GTCCCGGACT ATGCATACCC CTACGATGTA
     2281 CCCGATTACG CCggTGCAgg gAGTggaagt TAAATCCATA CAGaGTGTCA TCCTTCTTTC
     2341 CTTCAAGAAA CCTTTTTACA CATCTCCATT CCTTATTCCA CTTGGATTTC CTATAGCAAA
     2401 GAAACCCATT CATGTGTATG GAATCAACTG TTTATAGTCT TTTCACACTG CAGCTTTGGG
     2461 AAAACTTCAT TCCTTGATTT GTGTTTGTCT TGGCCTTCCT GGTGTGCAGT ACTGCTGTAG
     2521 AAAAGTATTA ATAGCTTCAT TTCATATAAA CATAAGTAAC TCCCAAACAC TTATGTAGAG
     2581 GACTAAAAAT GTATCTGGTA TTTAAGTAAT CTGAACCAGT TCTGCAAGTG ACTGTGTTTT
     2641 GTATTACTGT GAAAATAAGA AAATGTAGTT AATTACAATT TAAAGAGTAT TCCACATAAC
     2701 TTCTTAATTT CTACATTCCC TCCCTTACTC TTCGGGGGTT TCCTTTCAGT AAGCAACTTT
     2761 TCCATGCTCT TAATGTATTC CTTTTTAGTA GGAATCCGGA AGTATTAGAT TGAATGGAAA
     2821 AGCACTTGCC ATCTCTGTCT AGGGGTCACA AATTGAAATG GCTCCTGTAT CACATACGGA
     2881 GGTCTTGTGT ATCTGTGGCA ACAGGGAGTT TCCTTATTCA CTCTTTATTT GCTGCTGTTT
     2941 AAGTTGCCAA CCTCCCCTCC CAATAAAAAT TCACTTACAC CTCCTGCCTT TGTAGTTCTG
     3001 GTATTCACTT TACTATGTGA TAGAAGTAGC ATGTTGCTGC CAGAATACAA GCATTGCTTT
     3061 CGTCAGAAGA CCGTCAGTGA aggctaggtg gaggctcagt gatgataagt ctgcgatggt
     3121 ggatgcatgt gtcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcagagggc
     3181 acaatcctat tccgcgctat ccgacaatct ccaagacatt aggtggagtt cagttcggcg
     3241 tatggcatat gtcgctggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     3301 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     3361 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     3421 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     3481 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     3541 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     3601 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     3661 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     3721 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     3781 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     3841 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     3901 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc tattcaacaa
     3961 agccgccgtc ccgtcaagtc agcgtaaatg ggtagggggc ttcaaatcgt cctcgtgata
     4021 ccaattcgga gcctgctttt ttgtacaaac ttgttgataa tggcaattca aggatcttca
     4081 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     4141 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     4201 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     4261 taccatctgg ccccagtgct gcaatgatac cgcgagagcc acgctcaccg gctccagatt
     4321 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     4381 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     4441 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     4501 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     4561 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     4621 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     4681 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     4741 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     4801 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     4861 cgctgttgag atccagttcg atgtaaccca ctc
//