LOCUS       pML389_YWHAB-C-term_XTE 3993 bp ds-DNA     circular     24-SEP-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        complement(117..221)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     335..351
                     /label="M13 fwd"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    382..403
                     /label="Uni_9 F"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    404..424
                     /label="A5.2"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    425..724
                     /label="YWHAB 5' Homology arm"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    703..703
                     /label="Silent mutation"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    706..706
                     /label="Silent mutation"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    709..709
                     /label="Silent mutation"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    712..712
                     /label="Silent mutation"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    725..742
                     /label="5' Linker"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    725..1855
                     /label="Payload"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    743..982
                     /label="XTEN80"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    992..1015
                     /label="prescission"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             1031..1744
                     /label="mEGFP"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1684..1837
                     /label="payload"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    1757..1837
                     /label="3x HA Tag"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1838..1855
                     /label="3' Linker"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1856..2155
                     /label="YWHAB 3' Homology arm"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    complement(2156..2175)
                     /label="A3.2"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     source          2176..403
                     /label="source"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2176..2196
                     /label="Uni_9 R"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     complement(2232..2248)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     rep_origin      complement(2420..3008)
                     /label="ori"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    complement(3125..3149)
                     /label="attB1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             complement(3249..116)
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
       61 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
      121 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
      181 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
      241 ccagatacct gaaacaaaac ccatcgtacg gccaaggaag tctccaataa ctgtgatcca
      301 ccacaagcgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tcatgcataa
      361 tccgcacgca tctggaataa ggaagtgcca ttccgcctga cctGGAGCTT ACTGGTGTCA
      421 CTTCTTCACC TACTTAATAA TTCACTGTTA TCTTCAAGAG GGGATTTGTA CCATTAAATG
      481 TAGTTACAGT TTTAAGTTGT TAAATTTGTT ATAAATGACT GCTGAAGTTT TGACCTTTGC
      541 AATCATCCCT GTCTGCAGTG ACACAGGAAT AATTTTAAAC TGTCGAGTGA GATAAGATTA
      601 TACATACTGG GCCACTTACC TAGGGAACTT TTAGTAGCCC TGCTTTGATT ATACTTCCTT
      661 TCTCTTGCAG CTGTGGACAT CGGAAAACCA GGGAGACGAA GGcGAtGCcG GaGAGGGAGA
      721 GAACAGTGGT GGTACTTCCG GGGGAGGGCC GAGCTCTGGC GCACCCCCAC CAAGTGGAGG
      781 GTCACCTGCC GGGTCCCCAA CATCTACTGA AGAAGGCACC AGCGAATCCG CAACGCCCGA
      841 GTCAGGCCCT GGTACCTCCA CAGAACCATC TGAAGGTAGT GCGCCTGGTT CCCCAGCTGG
      901 AAGCCCTACT TCCACCGAAG AAGGCACGTC AACCGAACCA AGTGAAGGAT CTGCCCCTGG
      961 GACCAGCACT GAACCATCTG AGGGTGGCGG ATTGGAAGTT TTGTTTCAAG GTCCAGGATC
     1021 TAACGGCAGC gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     1081 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     1141 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     1201 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     1261 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     1321 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     1381 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     1441 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     1501 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     1561 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     1621 caaccactac ctgagcaccc agtccaagct gagcaaagac cccaacgaga agcgcgatca
     1681 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     1741 caagGCTGGA GCTGGCTACC CATACGATGT TCCAGATTAC GCTTATCCGT ATGACGTCCC
     1801 GGACTATGCA TACCCCTACG ATGTACCCGA TTACGCCggT GCAgggAGTg gaagtTAATG
     1861 TTTCTCGTGC TTTGTGATCT GTTCAGTGTC ACTCTGTACC CTCAACATAT ATCCCTTGTG
     1921 CGATaaaaaa aaaaaaaaaa aaaaaaaGAG AATCGTACGT CGACTTTCGA TTTTTCACAG
     1981 CCTCAGCCTA GGAAAAATGG TTCATGGGAT AAACAGCTGG TATTTGTATC TAAAACTCAG
     2041 ATTGGTCACA TAAATGCCAC GGCATTCCGA AGTTTTGATT TTGATTAACA TTGACAGGAT
     2101 TACTGTGTGT TTAATTTTTT AAAAACTGAA CACTGTGATT ATGGGGTTTT GTAATCGTCA
     2161 GAAGACCGTC AGTGAaggct aggtggaggc tcagtgatga taagtctgcg atggtggatg
     2221 catgtgtcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcag agggcacaat
     2281 cctattccgc gctatccgac aatctccaag acattaggtg gagttcagtt cggcgtatgg
     2341 catatgtcgc tggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     2401 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     2461 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     2521 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     2581 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     2641 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     2701 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     2761 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     2821 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     2881 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     2941 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     3001 gatctcaaga agatcctttg atcttttcta cggggtctga cgctctattc aacaaagccg
     3061 ccgtcccgtc aagtcagcgt aaatgggtag ggggcttcaa atcgtcctcg tgataccaat
     3121 tcggagcctg cttttttgta caaacttgtt gataatggca attcaaggat cttcacctag
     3181 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     3241 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     3301 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     3361 tctggcccca gtgctgcaat gataccgcga gagccacgct caccggctcc agatttatca
     3421 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     3481 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     3541 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     3601 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     3661 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     3721 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     3781 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     3841 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     3901 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     3961 ttgagatcca gttcgatgta acccactcgt gca
//