LOCUS       5455\pcDNA:mCit:        6496 bp    DNA     circular SYN 27-JUL-2021
DEFINITION  pcDNA3.
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-13973046|
COMMENT     VNTDBDATE|-13973046|
COMMENT     LSOWNER|
COMMENT     VNTNAME|5455 pcDNA:mCit:(SAH-S1-SAH-S3)1|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
FEATURES             Location/Qualifiers
     misc_feature    315..602
                     /vntifkey="21"
                     /label=CAG_enhancer
     promoter        236..852
                     /vntifkey="30"
                     /label=CMV_immearly_promoter
     misc_feature    769..789
                     /vntifkey="21"
                     /label=CMV_fwd_primer
     promoter        863..881
                     /vntifkey="30"
                     /label=T7_promoter
     misc_feature    complement(2049..2066)
                     /vntifkey="21"
                     /label=Sp6_primer
     promoter        complement(2050..2067)
                     /vntifkey="30"
                     /label=Sp6_promoter
     misc_feature    complement(2086..2103)
                     /vntifkey="21"
                     /label=BGH_rev_primer
     terminator      2089..2316
                     /vntifkey="43"
                     /label=bGH_PA_terminator
     rep_origin      2379..2685
                     /vntifkey="33"
                     /label=f1_origin
     misc_feature    complement(2799..2819)
                     /vntifkey="21"
                     /label=pBABE_3_primer
     misc_feature    complement(2805..3021)
                     /vntifkey="21"
                     /label=SV40_enhancer
     promoter        2817..3086
                     /vntifkey="30"
                     /label=SV40_promoter
     rep_origin      2985..3062
                     /vntifkey="33"
                     /label=SV40_origin
     misc_feature    3047..3066
                     /vntifkey="21"
                     /label=SV40pro_F_primer
     CDS             3201..3995
                     /gene="Neomycin"
                     /vntifkey="4"
                     /label=Neomycin
                     /note="ORF frame 3"
     gene            3204..3992
                     /gene="Neomycin"
                     /vntifkey="60"
     terminator      4172..4291
                     /vntifkey="43"
                     /label=SV40_PA_terminator
     misc_feature    4260..4279
                     /vntifkey="21"
                     /label=EBV_rev_primer
     misc_feature    complement(4335..4353)
                     /vntifkey="21"
                     /label=M13_reverse_primer
     misc_feature    complement(4352..4374)
                     /vntifkey="21"
                     /label=M13_pUC_rev_primer
     promoter        complement(4388..4417)
                     /vntifkey="30"
                     /label=lac_promoter
     rep_origin      complement(4726..5345)
                     /vntifkey="33"
                     /label=pBR322_origin
     CDS             complement(5500..6360)
                     /gene="Ampicillin"
                     /vntifkey="4"
                     /label=Ampicillin
                     /note="ORF frame 1"
     gene            complement(5500..6360)
                     /gene="Ampicillin"
                     /vntifkey="60"
     promoter        complement(6402..6430)
                     /vntifkey="30"
                     /label=AmpR_promoter
     primer_bind     2317..2346
                     /vntifkey="28"
                     /label=pimp\my\pCDNA3-\F
     misc_feature    206..235
                     /vntifkey="21"
                     /label=pimp\my\pCDNA3\-r
     misc_signal     944..949
                     /vntifkey="87"
                     /label=Kozakovo\zaporedje
     misc_feature    950..952
                     /vntifkey="21"
                     /label=start\kodon
     misc_marker     953..970
                     /vntifkey="22"
                     /label=HIS\tag
     misc_feature    971..1687
                     /vntifkey="21"
                     /label=mCitrine
     misc_feature    1796..1858
                     /vntifkey="21"
                     /label=S1
     misc_feature    1967..2029
                     /vntifkey="21"
                     /label=S3
BASE COUNT     1612 a      1679 c      1698 g      1507 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc 
      901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttcgccacca tgcaccacca 
      961 ccaccaccac atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt 
     1021 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga 
     1081 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc 
     1141 ctggcccacc ctcgtgacca ccttcggcta cggcctgatg tgcttcgccc gctaccccga 
     1201 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg 
     1261 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg 
     1321 cgacaccctg gtgaactgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat 
     1381 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa 
     1441 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt 
     1501 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc 
     1561 cgacaaccac tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga 
     1621 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct 
     1681 gtacaaggaa gaagaggaga agaaaaagaa agaggaggag gagaagaaga aaaaagaaga 
     1741 agaggaaaaa aaaaagaaag aggaggagga gaagaaaaag aaagaagagg aagagaaaat 
     1801 ccagtccctg aaagaggaaa ttagtcaatt ggagcagaaa aattctcagc tcaaagaaga 
     1861 ggaggaggaa aagaaaaaga aggaggaaga ggaaaagaag aaaaaggagg aagaagagaa 
     1921 gaagaagaag gaagaggaag agaagaaaaa gaaagaagaa gaggaagaga ttcaaagtct 
     1981 ggaagaggag aacagtcaac tggagcagaa gatatctcag ctcaaggagt aatctagagg 
     2041 gccctattct atagtgtcac ctaaatgcta gagctcgctg atcagcctcg actgtgcctt 
     2101 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg 
     2161 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt 
     2221 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca 
     2281 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct 
     2341 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg 
     2401 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt 
     2461 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcggggca 
     2521 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg 
     2581 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg 
     2641 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct 
     2701 cggtctattc ttttgattta taagggattt tggggatttc ggcctattgg ttaaaaaatg 
     2761 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg 
     2821 tggaaagtcc ccaggctccc caggcaggca gaagtatgca aagcatgcat ctcaattagt 
     2881 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 
     2941 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc 
     3001 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg 
     3061 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc 
     3121 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga 
     3181 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg 
     3241 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg 
     3301 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt 
     3361 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg 
     3421 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat 
     3481 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat 
     3541 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg 
     3601 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg 
     3661 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc 
     3721 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc 
     3781 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg 
     3841 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg 
     3901 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca 
     3961 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac 
     4021 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga 
     4081 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga 
     4141 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa 
     4201 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 
     4261 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta 
     4321 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat 
     4381 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag 
     4441 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg 
     4501 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 
     4561 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 
     4621 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 
     4681 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 
     4741 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 
     4801 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 
     4861 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 
     4921 cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 
     4981 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 
     5041 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 
     5101 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 
     5161 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 
     5221 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 
     5281 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 
     5341 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 
     5401 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 
     5461 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 
     5521 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 
     5581 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 
     5641 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 
     5701 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 
     5761 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 
     5821 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 
     5881 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 
     5941 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 
     6001 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 
     6061 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 
     6121 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 
     6181 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 
     6241 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 
     6301 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 
     6361 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 
     6421 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 
     6481 agtgccacct gacgtc 
//
