LOCUS       5481\pcDNA:mCit:        6670 bp    DNA     circular SYN 29-AUG-2022
DEFINITION  pcDNA3.
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-14546374|
COMMENT     VNTDBDATE|-14546374|
COMMENT     LSOWNER|
COMMENT     VNTNAME|5481 pcDNA:mCit:(gs-S1h-gs-S3h)3|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
FEATURES             Location/Qualifiers
     misc_feature    315..602
                     /vntifkey="21"
                     /label=CAG_enhancer
     promoter        236..852
                     /vntifkey="30"
                     /label=CMV_immearly_promoter
     misc_feature    769..789
                     /vntifkey="21"
                     /label=CMV_fwd_primer
     promoter        863..881
                     /vntifkey="30"
                     /label=T7_promoter
     misc_feature    complement(2223..2240)
                     /vntifkey="21"
                     /label=Sp6_primer
     promoter        complement(2224..2241)
                     /vntifkey="30"
                     /label=Sp6_promoter
     misc_feature    complement(2260..2277)
                     /vntifkey="21"
                     /label=BGH_rev_primer
     terminator      2263..2490
                     /vntifkey="43"
                     /label=bGH_PA_terminator
     rep_origin      2553..2859
                     /vntifkey="33"
                     /label=f1_origin
     misc_feature    complement(2973..2993)
                     /vntifkey="21"
                     /label=pBABE_3_primer
     misc_feature    complement(2979..3195)
                     /vntifkey="21"
                     /label=SV40_enhancer
     promoter        2991..3260
                     /vntifkey="30"
                     /label=SV40_promoter
     rep_origin      3159..3236
                     /vntifkey="33"
                     /label=SV40_origin
     misc_feature    3221..3240
                     /vntifkey="21"
                     /label=SV40pro_F_primer
     CDS             3375..4169
                     /gene="Neomycin"
                     /vntifkey="4"
                     /label=Neomycin
                     /note="ORF frame 3"
     gene            3378..4166
                     /gene="Neomycin"
                     /vntifkey="60"
     terminator      4346..4465
                     /vntifkey="43"
                     /label=SV40_PA_terminator
     misc_feature    4434..4453
                     /vntifkey="21"
                     /label=EBV_rev_primer
     misc_feature    complement(4509..4527)
                     /vntifkey="21"
                     /label=M13_reverse_primer
     misc_feature    complement(4526..4548)
                     /vntifkey="21"
                     /label=M13_pUC_rev_primer
     promoter        complement(4562..4591)
                     /vntifkey="30"
                     /label=lac_promoter
     rep_origin      complement(4900..5519)
                     /vntifkey="33"
                     /label=pBR322_origin
     CDS             complement(5674..6534)
                     /gene="Ampicillin"
                     /vntifkey="4"
                     /label=Ampicillin
                     /note="ORF frame 1"
     gene            complement(5674..6534)
                     /gene="Ampicillin"
                     /vntifkey="60"
     promoter        complement(6576..6604)
                     /vntifkey="30"
                     /label=AmpR_promoter
     primer_bind     2491..2520
                     /vntifkey="28"
                     /label=pimp\my\pCDNA3-\F
     misc_feature    206..235
                     /vntifkey="21"
                     /label=pimp\my\pCDNA3\-r
     misc_signal     944..949
                     /vntifkey="87"
                     /label=Kozakovo\zaporedje
     misc_feature    950..952
                     /vntifkey="21"
                     /label=start\kodon
     misc_marker     953..970
                     /vntifkey="22"
                     /label=HIS\tag
     misc_feature    971..1687
                     /vntifkey="21"
                     /label=mCitrine
     misc_feature    1688..2203
                     /vntifkey="21"
                     /label=(gs-S1h-gs-S3h)3
BASE COUNT     1578 a      1766 c      1766 g      1560 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc 
      901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttcgccacca tgcaccacca 
      961 ccaccaccac atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt 
     1021 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga 
     1081 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc 
     1141 ctggcccacc ctcgtgacca ccttcggcta cggcctgatg tgcttcgccc gctaccccga 
     1201 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg 
     1261 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg 
     1321 cgacaccctg gtgaactgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat 
     1381 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa 
     1441 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt 
     1501 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc 
     1561 cgacaaccac tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga 
     1621 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct 
     1681 gtacaagggc agtggatcag gagggtcaaa gattgcagcc ttgaaagaag aaatcgcagc 
     1741 ccttgagcaa aagaatgccg ctttgaagga aggatcagga tctgggcccg gtagcggcga 
     1801 aattgctgcg ctggaggaag aaaacgcggc acttgagcag aaaattgccg ccctgaagga 
     1861 gggaagtggc agtgggggtt ccaaaatagc agcgcttaag gaggaaattg ccgcactgga 
     1921 gcagaaaaat gcggccctca aagaaggctc aggtagtggt gggtccgaaa tcgccgcact 
     1981 cgaggaggaa aacgcggcgc ttgagcaaaa gatcgctgcc cttaaagagg gctccgggtc 
     2041 cggaccagga tccgggaaga tagctgcgct gaaggaagag attgcggctc tcgagcaaaa 
     2101 gaacgctgca ctgaaagagg ggagcgggtc agggggctct gagatcgcag cactggaaga 
     2161 agaaaatgct gcgctggagc agaaaatcgc agccttgaag gagtaatcta gagggcccta 
     2221 ttctatagtg tcacctaaat gctagagctc gctgatcagc ctcgactgtg ccttctagtt 
     2281 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc 
     2341 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt 
     2401 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca 
     2461 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct 
     2521 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta 
     2581 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc 
     2641 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg ggcatccctt 
     2701 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg 
     2761 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca 
     2821 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct 
     2881 attcttttga tttataaggg attttgggga tttcggccta ttggttaaaa aatgagctga 
     2941 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa 
     3001 gtccccaggc tccccaggca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa 
     3061 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca 
     3121 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca 
     3181 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg 
     3241 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct 
     3301 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat 
     3361 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg 
     3421 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg 
     3481 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg 
     3541 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc 
     3601 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg 
     3661 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca 
     3721 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc 
     3781 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg 
     3841 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg 
     3901 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata 
     3961 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg 
     4021 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat 
     4081 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct 
     4141 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca 
     4201 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt 
     4261 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat 
     4321 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag 
     4381 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 
     4441 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt 
     4501 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 
     4561 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact 
     4621 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 
     4681 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 
     4741 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 
     4801 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 
     4861 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 
     4921 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 
     4981 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 
     5041 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 
     5101 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 
     5161 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 
     5221 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 
     5281 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 
     5341 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 
     5401 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 
     5461 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 
     5521 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 
     5581 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 
     5641 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 
     5701 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 
     5761 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 
     5821 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 
     5881 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 
     5941 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 
     6001 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 
     6061 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 
     6121 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 
     6181 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 
     6241 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 
     6301 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 
     6361 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 
     6421 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 
     6481 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 
     6541 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 
     6601 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 
     6661 acctgacgtc 
//
