LOCUS       5486\pcDNA:BFP:(        6775 bp    DNA     circular SYN  2-NOV-2022
DEFINITION  pcDNA3.
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-14640042|
COMMENT     VNTDBDATE|-14640042|
COMMENT     LSOWNER|
COMMENT     VNTNAME|5486 pcDNA:BFP:(gs-P6f)3(gs-P14f)3|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
FEATURES             Location/Qualifiers
     misc_feature    315..602
                     /vntifkey="21"
                     /label=CAG_enhancer
     promoter        236..852
                     /vntifkey="30"
                     /label=CMV_immearly_promoter
     misc_feature    769..789
                     /vntifkey="21"
                     /label=CMV_fwd_primer
     promoter        863..881
                     /vntifkey="30"
                     /label=T7_promoter
     misc_feature    complement(2328..2345)
                     /vntifkey="21"
                     /label=Sp6_primer
     promoter        complement(2329..2346)
                     /vntifkey="30"
                     /label=Sp6_promoter
     misc_feature    complement(2365..2382)
                     /vntifkey="21"
                     /label=BGH_rev_primer
     terminator      2368..2595
                     /vntifkey="43"
                     /label=bGH_PA_terminator
     rep_origin      2658..2964
                     /vntifkey="33"
                     /label=f1_origin
     misc_feature    complement(3078..3098)
                     /vntifkey="21"
                     /label=pBABE_3_primer
     misc_feature    complement(3084..3300)
                     /vntifkey="21"
                     /label=SV40_enhancer
     promoter        3096..3365
                     /vntifkey="30"
                     /label=SV40_promoter
     rep_origin      3264..3341
                     /vntifkey="33"
                     /label=SV40_origin
     misc_feature    3326..3345
                     /vntifkey="21"
                     /label=SV40pro_F_primer
     CDS             3480..4274
                     /gene="Neomycin"
                     /vntifkey="4"
                     /label=Neomycin
                     /note="ORF frame 3"
     gene            3483..4271
                     /gene="Neomycin"
                     /vntifkey="60"
     terminator      4451..4570
                     /vntifkey="43"
                     /label=SV40_PA_terminator
     misc_feature    4539..4558
                     /vntifkey="21"
                     /label=EBV_rev_primer
     misc_feature    complement(4614..4632)
                     /vntifkey="21"
                     /label=M13_reverse_primer
     misc_feature    complement(4631..4653)
                     /vntifkey="21"
                     /label=M13_pUC_rev_primer
     promoter        complement(4667..4696)
                     /vntifkey="30"
                     /label=lac_promoter
     rep_origin      complement(5005..5624)
                     /vntifkey="33"
                     /label=pBR322_origin
     CDS             complement(5779..6639)
                     /gene="Ampicillin"
                     /vntifkey="4"
                     /label=Ampicillin
                     /note="ORF frame 1"
     gene            complement(5779..6639)
                     /gene="Ampicillin"
                     /vntifkey="60"
     promoter        complement(6681..6709)
                     /vntifkey="30"
                     /label=AmpR_promoter
     primer_bind     2596..2625
                     /vntifkey="28"
                     /label=pimp\my\pCDNA3-\F
     misc_feature    206..235
                     /vntifkey="21"
                     /label=pimp\my\pCDNA3\-r
     misc_signal     944..949
                     /vntifkey="87"
                     /label=Kozakovo\zaporedje
     misc_feature    950..952
                     /vntifkey="21"
                     /label=start\kodon
     misc_marker     953..970
                     /vntifkey="22"
                     /label=HIS\tag
     misc_feature    971..1669
                     /vntifkey="21"
                     /label=BFP
     misc_feature    1670..2308
                     /vntifkey="21"
                     /label=(gs-P6f)3(gs-P14f)3
BASE COUNT     1644 a      1753 c      1786 g      1592 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc 
      901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttcgccacca tgcaccacca 
      961 ccaccaccac atgagcgagc tgattaagga gaacatgcac atgaagctgt acatggaggg 
     1021 caccgtggac aaccatcact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg 
     1081 cacccagacc atgagaatca aggtggtcga gggcggccct ctccccttcg ccttcgacat 
     1141 cctggctact agcttcctct acggcagcaa gaccttcatc aaccacaccc agggcatccc 
     1201 cgacttcttc aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga 
     1261 agacgggggc gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta 
     1321 caacgtcaag atcagagggg tgaacttcac atccaacggc cctgtgatgc agaagaaaac 
     1381 actcggctgg gaggccttca ccgagacgct gtaccccgct gacggcggcc tggaaggcag 
     1441 aaacgacatg gccctgaagc tcgtgggcgg gagccatctg atcgcaaaca tcaagaccac 
     1501 atatagatcc aagaaacccg ctaagaacct caagatgcct ggcgtctact atgtggacta 
     1561 cagactggaa agaatcaagg aggccaacaa cgagacctac gtcgagcagc acgaggtggc 
     1621 agtggccaga tactgcgacc tccctagcaa actggggcac aagctcaatg gctcaggctc 
     1681 tggtgggagc aaaaacgcag ccttgaaaga agaaattgcg gctctggaag aggagaatgc 
     1741 ggcccttgaa gaaaaaatag ctgcactgaa ggccggtagt ggaagtgggg gttcaaagaa 
     1801 tgcggcattg aaggaagaga ttgctgcctt ggaggaggag aatgccgcgc tcgaggaaaa 
     1861 gatcgcagct ttgaaagcgg gatctggatc aggtggcagc aaaaatgctg ctctgaaaga 
     1921 ggaaatagca gctctggagg aggagaacgc cgccctcgag gaaaaaattg ccgccttgaa 
     1981 agcgggcggc tctggcggcg gctccggagg ctctgaaatt gcagctctcg aggagaagat 
     2041 tgcagccctg aagcaggaga atgcagccct ggaagagaaa aatgcggcgt tgaaggctgg 
     2101 aagtggtagt gggggatccg aaattgctgc gttggaagag aagatagcgg cgcttaaaca 
     2161 agaaaatgca gccctcgaag aaaaaaatgc tgcacttaaa gcggggtcag gatcaggtgg 
     2221 ttcagagatc gccgctcttg aagagaaaat agccgcattg aagcaagaga acgcagcctt 
     2281 ggaggaaaaa aacgctgcgc tgaaagcata atctagaggg ccctattcta tagtgtcacc 
     2341 taaatgctag agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg 
     2401 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct 
     2461 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg 
     2521 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg 
     2581 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc 
     2641 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 
     2701 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 
     2761 cgttcgccgg ctttccccgt caagctctaa atcggggcat ccctttaggg ttccgattta 
     2821 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 
     2881 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 
     2941 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 
     3001 aagggatttt ggggatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 
     3061 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc 
     3121 aggcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt 
     3181 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca 
     3241 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc 
     3301 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg 
     3361 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc 
     3421 cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca 
     3481 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg 
     3541 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag 
     3601 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc 
     3661 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc 
     3721 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg 
     3781 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc 
     3841 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca 
     3901 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag 
     3961 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg 
     4021 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg 
     4081 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca 
     4141 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc 
     4201 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg 
     4261 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct 
     4321 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt 
     4381 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc 
     4441 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 
     4501 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 
     4561 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc 
     4621 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg 
     4681 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt 
     4741 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg 
     4801 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 
     4861 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 
     4921 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 
     4981 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 
     5041 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 
     5101 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 
     5161 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc 
     5221 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 
     5281 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 
     5341 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 
     5401 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 
     5461 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 
     5521 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 
     5581 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 
     5641 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 
     5701 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga 
     5761 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 
     5821 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga 
     5881 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 
     5941 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 
     6001 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 
     6061 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc 
     6121 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc 
     6181 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 
     6241 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 
     6301 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg 
     6361 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag 
     6421 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat 
     6481 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc 
     6541 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 
     6601 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta 
     6661 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa 
     6721 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc 
//
