LOCUS       5504\pcDNA:(gs-P        6076 bp    DNA     circular SYN 14-FEB-2023
DEFINITION  pcDNA3.
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-14789485|
COMMENT     VNTDBDATE|-14789485|
COMMENT     LSOWNER|
COMMENT     VNTNAME|5504 pcDNA:(gs-P6f)3(gs-P14f)3|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
FEATURES             Location/Qualifiers
     misc_feature    315..602
                     /vntifkey="21"
                     /label=CAG_enhancer
     promoter        236..852
                     /vntifkey="30"
                     /label=CMV_immearly_promoter
     misc_feature    769..789
                     /vntifkey="21"
                     /label=CMV_fwd_primer
     promoter        863..881
                     /vntifkey="30"
                     /label=T7_promoter
     misc_feature    complement(1629..1646)
                     /vntifkey="21"
                     /label=Sp6_primer
     promoter        complement(1630..1647)
                     /vntifkey="30"
                     /label=Sp6_promoter
     misc_feature    complement(1666..1683)
                     /vntifkey="21"
                     /label=BGH_rev_primer
     terminator      1669..1896
                     /vntifkey="43"
                     /label=bGH_PA_terminator
     rep_origin      1959..2265
                     /vntifkey="33"
                     /label=f1_origin
     misc_feature    complement(2379..2399)
                     /vntifkey="21"
                     /label=pBABE_3_primer
     misc_feature    complement(2385..2601)
                     /vntifkey="21"
                     /label=SV40_enhancer
     promoter        2397..2666
                     /vntifkey="30"
                     /label=SV40_promoter
     rep_origin      2565..2642
                     /vntifkey="33"
                     /label=SV40_origin
     misc_feature    2627..2646
                     /vntifkey="21"
                     /label=SV40pro_F_primer
     CDS             2781..3575
                     /gene="Neomycin"
                     /vntifkey="4"
                     /label=Neomycin
                     /note="ORF frame 3"
     gene            2784..3572
                     /gene="Neomycin"
                     /vntifkey="60"
     terminator      3752..3871
                     /vntifkey="43"
                     /label=SV40_PA_terminator
     misc_feature    3840..3859
                     /vntifkey="21"
                     /label=EBV_rev_primer
     misc_feature    complement(3915..3933)
                     /vntifkey="21"
                     /label=M13_reverse_primer
     misc_feature    complement(3932..3954)
                     /vntifkey="21"
                     /label=M13_pUC_rev_primer
     promoter        complement(3968..3997)
                     /vntifkey="30"
                     /label=lac_promoter
     rep_origin      complement(4306..4925)
                     /vntifkey="33"
                     /label=pBR322_origin
     CDS             complement(5080..5940)
                     /gene="Ampicillin"
                     /vntifkey="4"
                     /label=Ampicillin
                     /note="ORF frame 1"
     gene            complement(5080..5940)
                     /gene="Ampicillin"
                     /vntifkey="60"
     promoter        complement(5982..6010)
                     /vntifkey="30"
                     /label=AmpR_promoter
     primer_bind     1897..1926
                     /vntifkey="28"
                     /label=pimp\my\pCDNA3-\F
     misc_feature    206..235
                     /vntifkey="21"
                     /label=pimp\my\pCDNA3\-r
     misc_signal     944..949
                     /vntifkey="87"
                     /label=Kozakovo\zaporedje
     misc_feature    950..952
                     /vntifkey="21"
                     /label=start\kodon
     misc_marker     953..970
                     /vntifkey="22"
                     /label=HIS\tag
     misc_feature    971..1609
                     /vntifkey="21"
                     /label=(gs-P6f)3(gs-P14f)3
BASE COUNT     1453 a      1542 c      1599 g      1482 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc 
      901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttcgccacca tgcaccacca 
      961 ccaccaccac ggctcaggct ctggtgggag caaaaacgca gccttgaaag aagaaattgc 
     1021 ggctctggaa gaggagaatg cggcccttga agaaaaaata gctgcactga aggccggtag 
     1081 tggaagtggg ggttcaaaga atgcggcatt gaaggaagag attgctgcct tggaggagga 
     1141 gaatgccgcg ctcgaggaaa agatcgcagc tttgaaagcg ggatctggat caggtggcag 
     1201 caaaaatgct gctctgaaag aggaaatagc agctctggag gaggagaacg ccgccctcga 
     1261 ggaaaaaatt gccgccttga aagcgggcgg ctctggcggc ggctccggag gctctgaaat 
     1321 tgcagctctc gaggagaaga ttgcagccct gaagcaggag aatgcagccc tggaagagaa 
     1381 aaatgcggcg ttgaaggctg gaagtggtag tgggggatcc gaaattgctg cgttggaaga 
     1441 gaagatagcg gcgcttaaac aagaaaatgc agccctcgaa gaaaaaaatg ctgcacttaa 
     1501 agcggggtca ggatcaggtg gttcagagat cgccgctctt gaagagaaaa tagccgcatt 
     1561 gaagcaagag aacgcagcct tggaggaaaa aaacgctgcg ctgaaagcat aatctagagg 
     1621 gccctattct atagtgtcac ctaaatgcta gagctcgctg atcagcctcg actgtgcctt 
     1681 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg 
     1741 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt 
     1801 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca 
     1861 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct 
     1921 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg 
     1981 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt 
     2041 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcggggca 
     2101 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg 
     2161 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg 
     2221 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct 
     2281 cggtctattc ttttgattta taagggattt tggggatttc ggcctattgg ttaaaaaatg 
     2341 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg 
     2401 tggaaagtcc ccaggctccc caggcaggca gaagtatgca aagcatgcat ctcaattagt 
     2461 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 
     2521 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc 
     2581 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg 
     2641 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc 
     2701 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga 
     2761 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg 
     2821 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg 
     2881 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt 
     2941 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg 
     3001 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat 
     3061 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat 
     3121 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg 
     3181 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg 
     3241 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc 
     3301 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc 
     3361 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg 
     3421 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg 
     3481 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca 
     3541 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac 
     3601 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga 
     3661 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga 
     3721 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa 
     3781 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 
     3841 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta 
     3901 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat 
     3961 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag 
     4021 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg 
     4081 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 
     4141 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 
     4201 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 
     4261 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 
     4321 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 
     4381 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 
     4441 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 
     4501 cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 
     4561 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 
     4621 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 
     4681 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 
     4741 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 
     4801 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 
     4861 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 
     4921 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 
     4981 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 
     5041 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 
     5101 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 
     5161 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 
     5221 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 
     5281 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 
     5341 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 
     5401 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 
     5461 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 
     5521 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 
     5581 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 
     5641 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 
     5701 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 
     5761 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 
     5821 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 
     5881 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 
     5941 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 
     6001 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 
     6061 agtgccacct gacgtc 
//
