LOCUS       5482\pcDNA:mCit:        6823 bp    DNA     circular SYN  7-OCT-2022
DEFINITION  pcDNA3.
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-14602530|
COMMENT     VNTDBDATE|-14602530|
COMMENT     LSOWNER|
COMMENT     VNTNAME|5482 pcDNA:mCit:S1hGS8|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
FEATURES             Location/Qualifiers
     misc_feature    315..602
                     /vntifkey="21"
                     /label=CAG_enhancer
     promoter        236..852
                     /vntifkey="30"
                     /label=CMV_immearly_promoter
     misc_feature    769..789
                     /vntifkey="21"
                     /label=CMV_fwd_primer
     promoter        863..881
                     /vntifkey="30"
                     /label=T7_promoter
     misc_feature    complement(2376..2393)
                     /vntifkey="21"
                     /label=Sp6_primer
     promoter        complement(2377..2394)
                     /vntifkey="30"
                     /label=Sp6_promoter
     misc_feature    complement(2413..2430)
                     /vntifkey="21"
                     /label=BGH_rev_primer
     terminator      2416..2643
                     /vntifkey="43"
                     /label=bGH_PA_terminator
     rep_origin      2706..3012
                     /vntifkey="33"
                     /label=f1_origin
     misc_feature    complement(3126..3146)
                     /vntifkey="21"
                     /label=pBABE_3_primer
     misc_feature    complement(3132..3348)
                     /vntifkey="21"
                     /label=SV40_enhancer
     promoter        3144..3413
                     /vntifkey="30"
                     /label=SV40_promoter
     rep_origin      3312..3389
                     /vntifkey="33"
                     /label=SV40_origin
     misc_feature    3374..3393
                     /vntifkey="21"
                     /label=SV40pro_F_primer
     CDS             3528..4322
                     /gene="Neomycin"
                     /vntifkey="4"
                     /label=Neomycin
                     /note="ORF frame 3"
     gene            3531..4319
                     /gene="Neomycin"
                     /vntifkey="60"
     terminator      4499..4618
                     /vntifkey="43"
                     /label=SV40_PA_terminator
     misc_feature    4587..4606
                     /vntifkey="21"
                     /label=EBV_rev_primer
     misc_feature    complement(4662..4680)
                     /vntifkey="21"
                     /label=M13_reverse_primer
     misc_feature    complement(4679..4701)
                     /vntifkey="21"
                     /label=M13_pUC_rev_primer
     promoter        complement(4715..4744)
                     /vntifkey="30"
                     /label=lac_promoter
     rep_origin      complement(5053..5672)
                     /vntifkey="33"
                     /label=pBR322_origin
     CDS             complement(5827..6687)
                     /gene="Ampicillin"
                     /vntifkey="4"
                     /label=Ampicillin
                     /note="ORF frame 1"
     gene            complement(5827..6687)
                     /gene="Ampicillin"
                     /vntifkey="60"
     promoter        complement(6729..6757)
                     /vntifkey="30"
                     /label=AmpR_promoter
     primer_bind     2644..2673
                     /vntifkey="28"
                     /label=pimp\my\pCDNA3-\F
     misc_feature    206..235
                     /vntifkey="21"
                     /label=pimp\my\pCDNA3\-r
     misc_signal     944..949
                     /vntifkey="87"
                     /label=Kozakovo\zaporedje
     misc_feature    950..952
                     /vntifkey="21"
                     /label=start\kodon
     misc_marker     953..970
                     /vntifkey="22"
                     /label=HIS\tag
     misc_feature    971..1687
                     /vntifkey="21"
                     /label=mCitrine
     misc_feature    1688..2023
                     /vntifkey="21"
                     /label=S1hGS4
     misc_feature    2042..2356
                     /vntifkey="21"
                     /label=S1gs4
     misc_feature    2024..2041
                     /vntifkey="21"
                     /label=gs6
BASE COUNT     1635 a      1782 c      1803 g      1603 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc 
      901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttcgccacca tgcaccacca 
      961 ccaccaccac atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt 
     1021 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga 
     1081 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc 
     1141 ctggcccacc ctcgtgacca ccttcggcta cggcctgatg tgcttcgccc gctaccccga 
     1201 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg 
     1261 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg 
     1321 cgacaccctg gtgaactgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat 
     1381 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa 
     1441 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt 
     1501 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc 
     1561 cgacaaccac tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga 
     1621 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct 
     1681 gtacaagggc tcaggctctg gtgggagcaa aattgcggcg cttaaggaag agattgcagc 
     1741 tttggagcaa aaaaacgctg cgttgaaaga gggatcaggg tctggtggaa gtaaaattgc 
     1801 ggcacttaag gaagaaattg cagccttgga gcagaagaac gcagccctca aggaaggcag 
     1861 cggttctggt gggtctaaaa tcgctgcgct taaagaagag atagcggcat tggaacagaa 
     1921 aaatgcggcc cttaaggagg gcagtggatc tggcggctct aagatagccg ccttgaagga 
     1981 agagattgcc gcactggagc aaaaaaatgc agcactcaaa gagggcggct ctggaggctc 
     2041 taaaattgcg gcgcttaagg aagagattgc agctttggag caaaaaaacg ctgcgttgaa 
     2101 agagggatca gggtctggtg gaagtaaaat tgcggcactt aaggaagaaa ttgcagcctt 
     2161 ggagcagaag aacgcagccc tcaaggaagg cagcggttct ggtgggtcta aaatcgctgc 
     2221 gcttaaagaa gagatagcgg cattggaaca gaaaaatgcg gcccttaagg agggcagtgg 
     2281 atctggcggc tctaagatag ccgccttgaa ggaagagatt gccgcactgg agcaaaaaaa 
     2341 tgcagcactc aaagagtaat ctagagggcc ctattctata gtgtcaccta aatgctagag 
     2401 ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc 
     2461 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg 
     2521 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg 
     2581 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta 
     2641 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta 
     2701 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca 
     2761 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct 
     2821 ttccccgtca agctctaaat cggggcatcc ctttagggtt ccgatttagt gctttacggc 
     2881 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat 
     2941 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc 
     3001 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgg 
     3061 ggatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat 
     3121 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag gcaggcagaa 
     3181 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc 
     3241 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc 
     3301 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct 
     3361 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga 
     3421 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta 
     3481 tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag 
     3541 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg 
     3601 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc 
     3661 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag 
     3721 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca 
     3781 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat 
     3841 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata 
     3901 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac 
     3961 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc 
     4021 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg 
     4081 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg 
     4141 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta 
     4201 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg 
     4261 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct 
     4321 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga 
     4381 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc 
     4441 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt 
     4501 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 
     4561 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca 
     4621 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc 
     4681 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 
     4741 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 
     4801 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg 
     4861 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 
     4921 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 
     4981 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 
     5041 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 
     5101 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 
     5161 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 
     5221 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta 
     5281 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 
     5341 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 
     5401 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 
     5461 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 
     5521 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 
     5581 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 
     5641 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 
     5701 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 
     5761 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 
     5821 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 
     5881 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 
     5941 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 
     6001 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 
     6061 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 
     6121 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 
     6181 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 
     6241 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 
     6301 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 
     6361 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 
     6421 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 
     6481 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 
     6541 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 
     6601 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 
     6661 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 
     6721 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 
     6781 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtc 
//
