LOCUS       5495\pcDNA:FRB:g        7330 bp    DNA     circular SYN 15-JUL-2023
DEFINITION  pcDNA3.
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-15006968|
COMMENT     VNTDBDATE|-15006968|
COMMENT     LSOWNER|
COMMENT     VNTNAME|5495 pcDNA:FRB:gs10:mCit:APPPAPAPPAP|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
FEATURES             Location/Qualifiers
     misc_feature    315..602
                     /vntifkey="21"
                     /label=CAG_enhancer
     promoter        236..852
                     /vntifkey="30"
                     /label=CMV_immearly_promoter
     misc_feature    769..789
                     /vntifkey="21"
                     /label=CMV_fwd_primer
     promoter        863..881
                     /vntifkey="30"
                     /label=T7_promoter
     misc_feature    complement(2883..2900)
                     /vntifkey="21"
                     /label=Sp6_primer
     promoter        complement(2884..2901)
                     /vntifkey="30"
                     /label=Sp6_promoter
     misc_feature    complement(2920..2937)
                     /vntifkey="21"
                     /label=BGH_rev_primer
     terminator      2923..3150
                     /vntifkey="43"
                     /label=bGH_PA_terminator
     rep_origin      3213..3519
                     /vntifkey="33"
                     /label=f1_origin
     misc_feature    complement(3633..3653)
                     /vntifkey="21"
                     /label=pBABE_3_primer
     misc_feature    complement(3639..3855)
                     /vntifkey="21"
                     /label=SV40_enhancer
     promoter        3651..3920
                     /vntifkey="30"
                     /label=SV40_promoter
     rep_origin      3819..3896
                     /vntifkey="33"
                     /label=SV40_origin
     misc_feature    3881..3900
                     /vntifkey="21"
                     /label=SV40pro_F_primer
     CDS             4035..4829
                     /gene="Neomycin"
                     /vntifkey="4"
                     /label=Neomycin
                     /note="ORF frame 3"
     gene            4038..4826
                     /gene="Neomycin"
                     /vntifkey="60"
     terminator      5006..5125
                     /vntifkey="43"
                     /label=SV40_PA_terminator
     misc_feature    5094..5113
                     /vntifkey="21"
                     /label=EBV_rev_primer
     misc_feature    complement(5169..5187)
                     /vntifkey="21"
                     /label=M13_reverse_primer
     misc_feature    complement(5186..5208)
                     /vntifkey="21"
                     /label=M13_pUC_rev_primer
     promoter        complement(5222..5251)
                     /vntifkey="30"
                     /label=lac_promoter
     rep_origin      complement(5560..6179)
                     /vntifkey="33"
                     /label=pBR322_origin
     CDS             complement(6334..7194)
                     /gene="Ampicillin"
                     /vntifkey="4"
                     /label=Ampicillin
                     /note="ORF frame 1"
     gene            complement(6334..7194)
                     /gene="Ampicillin"
                     /vntifkey="60"
     promoter        complement(7236..7264)
                     /vntifkey="30"
                     /label=AmpR_promoter
     primer_bind     3151..3180
                     /vntifkey="28"
                     /label=pimp\my\pCDNA3-\F
     misc_feature    206..235
                     /vntifkey="21"
                     /label=pimp\my\pCDNA3\-r
     misc_signal     944..949
                     /vntifkey="87"
                     /label=Kozakovo\zaporedje
     misc_feature    950..952
                     /vntifkey="21"
                     /label=start\kodon
     misc_marker     953..970
                     /vntifkey="22"
                     /label=HIS\tag
     misc_feature    974..1261
                     /vntifkey="21"
                     /label=FRB
     misc_feature    1262..1291
                     /vntifkey="21"
                     /label=gs10
     misc_feature    2006..2863
                     /vntifkey="21"
                     /label=APPAPAPPAP
BASE COUNT     1752 a      1901 c      1967 g      1710 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc 
      901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttcgccacca tgcaccacca 
      961 ccaccaccac atggcttcta gaatcctctg gcatgagatg tggcatgaag gcctggaaga 
     1021 ggcatctcgt ttgtactttg gggaaaggaa cgtgaaaggc atgtttgagg tgctggagcc 
     1081 cttgcatgct atgatggaac ggggccccca gactctgaag gaaacatcct ttaatcaggc 
     1141 ctatggtcga gatttaatgg aggcccaaga gtggtgcagg aagtacatga aatcagggaa 
     1201 tgtcaaggac ctcctccaag cctgggacct ctattatcat gtgttccgac gaatctcaaa 
     1261 gggcggctct ggcggcggct ccggaggctc tgtgagcaag ggcgaggagc tgttcaccgg 
     1321 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc 
     1381 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac 
     1441 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcctgatgtg 
     1501 cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga 
     1561 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc 
     1621 cgaggtgaag ttcgagggcg acaccctggt gaactgcatc gagctgaagg gcatcgactt 
     1681 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt 
     1741 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa 
     1801 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga 
     1861 cggccccgtg ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga 
     1921 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac 
     1981 tctcggcatg gacgagctgt acaagggctc aggctctggt gggagcgaat tgcaagcgat 
     2041 cgaggagcaa cttgcgcagt tgcagtggaa ggctcaagct cgaaaggagg gcagtggaag 
     2101 tggtgggagt aaagttgagg agcttctcag taagaactac cacctggaga atgaggttgc 
     2161 tcgactgaaa aagggctccg ggagtggagg gtcaaaagtg gaagagctcc tctcaaagaa 
     2221 ctaccatctt gaaaatgaag ttgcccgcct gaaaaaggga agcggctcag gcgggagtga 
     2281 gcttcaggct attgaggaac agctggcgca acttcaatgg aaggcccaag cccgaaaaga 
     2341 ggggtctggc tccggtggtt ccaaagtaga agagttgctt agcaaaaact atcacctcga 
     2401 aaacgaagtc gccaggctta agaagggcgg ctctggaggc tctggctcag gctctggtgg 
     2461 gagcgaattg caagcgatcg aggagcaact tgcgcagttg cagtggaagg ctcaagctcg 
     2521 aaaggagggc agtggaagtg gtgggagtaa agttgaggag cttctcagta agaactacca 
     2581 cctggagaat gaggttgctc gactgaaaaa gggctccggg agtggagggt caaaagtgga 
     2641 agagctcctc tcaaagaact accatcttga aaatgaagtt gcccgcctga aaaagggaag 
     2701 cggctcaggc gggagtgagc ttcaggctat tgaggaacag ctggcgcaac ttcaatggaa 
     2761 ggcccaagcc cgaaaagagg ggtctggctc cggtggttcc aaagtagaag agttgcttag 
     2821 caaaaactat cacctcgaaa acgaagtcgc caggcttaag aagtaatcta gagggcccta 
     2881 ttctatagtg tcacctaaat gctagagctc gctgatcagc ctcgactgtg ccttctagtt 
     2941 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc 
     3001 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt 
     3061 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca 
     3121 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct 
     3181 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta 
     3241 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc 
     3301 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg ggcatccctt 
     3361 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg 
     3421 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca 
     3481 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct 
     3541 attcttttga tttataaggg attttgggga tttcggccta ttggttaaaa aatgagctga 
     3601 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa 
     3661 gtccccaggc tccccaggca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa 
     3721 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca 
     3781 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca 
     3841 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg 
     3901 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct 
     3961 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat 
     4021 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg 
     4081 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg 
     4141 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg 
     4201 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc 
     4261 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg 
     4321 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca 
     4381 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc 
     4441 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg 
     4501 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg 
     4561 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata 
     4621 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg 
     4681 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat 
     4741 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct 
     4801 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca 
     4861 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt 
     4921 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat 
     4981 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag 
     5041 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 
     5101 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt 
     5161 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 
     5221 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact 
     5281 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 
     5341 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 
     5401 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 
     5461 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 
     5521 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 
     5581 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 
     5641 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 
     5701 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 
     5761 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 
     5821 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 
     5881 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 
     5941 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 
     6001 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 
     6061 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 
     6121 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 
     6181 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 
     6241 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 
     6301 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 
     6361 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 
     6421 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 
     6481 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 
     6541 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 
     6601 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 
     6661 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 
     6721 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 
     6781 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 
     6841 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 
     6901 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 
     6961 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 
     7021 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 
     7081 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 
     7141 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 
     7201 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 
     7261 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 
     7321 acctgacgtc 
//
