LOCUS       UA_gadX_CFP_gadX_YFP    6289 bp ds-DNA     circular     03-OCT-2024
DEFINITION  .
COMMENT     Circularized  GFP_mut_2
FEATURES             Location/Qualifiers
     misc_feature    675..780
                     /label="T0"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(806..1600)
                     /label="KanR"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1817..1882
                     /label="gadX_prom"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1883..2385
                     /label="gadX_prom"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    2398..2423
                     /label="RBS"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     gene            2424..3155
                     /label="cfp"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    3185..3264
                     /label="terminator BBa B0010"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(3467..3546)
                     /label="terminator BBa B0010"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(3576..4292)
                     /label="YFP"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    4293..4318
                     /label="RBS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(4331..4833)
                     /label="gadX_prom"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(4834..4899)
                     /label="gadX_prom"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    5258..6208
                     /label="pSC101"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
ORIGIN
        1 TTGAGTTGTG GGTATCTGTA AATTCTGCTA GACCTTTGCT GGAAAACTTG TAAATTCTGC
       61 TAGACCCTCT GTAAATTCCG CTAGACCTTT GTGTGTTTTT TTTGTTTATA TTCAAGTGGT
      121 TATAATTTAT AGAATAAAGA AAGAATAAAA AAAGATAAAA AGAATAGATC CCAGCCCTGT
      181 GTATAACTCA CTACTTTAGT CAGTTCCGCA GTATTACAAA AGGATGTCGC AAACGCTGTT
      241 TGCTCCTCTA CAAAACAGAC CTTAAAACCC TAAAGGCTTA AGTAGCGCCG TCGCAAGCTC
      301 CGGCAAATCG CTGAATATTC CTTTTGTCTC CGACCATCAG GCACCTGAGT CGCTGTCTTT
      361 TTCGTCACAT TCAGTTCGCT GCGCTCACGG CTCTGGCAGT GAATGGGGGT AAATGGCACT
      421 ACAGGCGCCT TTTATGGATT CATGCAAGGA AACTACCCAT AATACAAGAA AAGCCCGTCA
      481 CGGGCTTCTC AGGGCGTTTT ATGGCGGGTC TGCTATGTGG TGCTATCTGA CTTTTTGCTG
      541 TTCAGCAGTT CCTGCCCTCT GATTTTCCAG TCTGACCACT TCGGATTATC CCGTGACAGG
      601 TCATTCAGAC TGGCTAATGC ACCCAGTAAG GCAGCGGTAT CATCAACAGG CTTACCCGTC
      661 TTACTGTCCC TAGTGCTTGG ATTCTCACCA ATAAAAAACG CCCGGCGGCA ACCGAGCGTT
      721 CTGAACAAAT CCAGATGGAG TTCTGAGGTC ATTACTGGAT CTATCAACAG GAGTCCAAGC
      781 GAGCTCTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA
      841 TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC
      901 CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG CGGTCCGCCA
      961 CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG
     1021 GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG CGCGCCTTGA
     1081 GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT
     1141 CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT
     1201 CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG
     1261 ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA
     1321 ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC
     1381 CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCCTG CAGTTCATTC AGGGCACCGG
     1441 ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG
     1501 CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG
     1561 CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG
     1621 TCTCTTGATC AGATCTTGAT CCCCTGCGCC ATCAGATCCT TGGCGGCAAG AAAGCCATCC
     1681 AGTTTACTTT GCAGGGCTTC CCAACCTTAC CAGAGGGCGC CCCAGCTGGC AATTCCGACG
     1741 TCTAAGAAAC CATTATTATC ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT
     1801 TTCGTCTTCA CCTCGATGGA TCCTGCTGGA AGACTACAAA GCCTCCCTGA AATATCTCAG
     1861 CGATCACCCG AAACTGCAGG GTattgccca gcagaacagc tttaaacaca cctgataaca
     1921 taacgttgta aaaaccgaat gcccagcctt taaaaaaaca gctgggcatt cggttgctta
     1981 ttaatcgcaa taatatattg gctgtctatt catcgtgttg ataagatata tgttatatga
     2041 atgtttatta aatgtagcga ttacctttaa tgtcacgaaa agggattatt tgcttactat
     2101 taatttccct gtgttcttaa acacgttttt gtattataaa tccagtcatc ctgcccgtag
     2161 aacatcacac attatcatcc tgttctcccg cttcgtttaa atttatttat caatcaattt
     2221 gacttaagag ggcggcgtgc tacattaata aacagtaata tgtttatgta atattaagtc
     2281 aactatgcaa tcactacatg ggaattgtct aattgcgtat gcaagacata aatatattct
     2341 caccatggtt aatggtgaat atcgctattt taatggcggt gacctCTCGA GAGATCCTCT
     2401 AGATTTAAGA AGGAGATATA CATatgacta gcaaaagaag caaaggtgaa gaactgttca
     2461 ctggtgttgt tccaattctg gttgaactgg atggtgatgt taatggtcac aaattttctg
     2521 tctctggtga gggtgaaggt gatgcaacct acggtaaact gaccctgaaa tttatttgca
     2581 ctactggtaa actgcctgtt ccgtggccaa ccctggtcac tactctgact tggggtgttc
     2641 aatgctttgc tcgttaccca gatcacatga aacagcatga ctttttcaag tctgccatgc
     2701 cggaaggtta tgttcaggaa cgtactatct ttttcaaaga tgacggtaac tacaagaccc
     2761 gtgctgaagt caagtttgaa ggtgataccc tggttaatcg tatcgagctg aaaggtattg
     2821 attttaaaga agatggtaac attctgggtc acaaactgga atacaacgct atttctgata
     2881 atgtatacat cactgctgac aaacaaaaga atggtatcaa agctaatttc aaaattcgtc
     2941 acaacattga agatggtagc gttcaactgg cagaccatta tcaacaaaat actccaattg
     3001 gcgatggccc tgtcctgctg ccagacaacc attacctgtc cacccaatct cgtctgtcta
     3061 aagatccgaa cgaaaagcgc gatcacatgg tcctgctgga gtttgtaacc gctgctggta
     3121 ttaccctggg catggatgaa ctgtataaat aatagATGTC CAGACCTGCA GGCATGCAAG
     3181 CTCTAGAGGC ATCAAATAAA ACGAAAGGCT CAGTCGAAAG ACTGGGCCTT TCGTTTTATC
     3241 TGTTGTTTGT CGGTGAACGC TCTCCTGAGT AGGACAAATC CGCCGCCCTA GACCTAGGGT
     3301 ACGGGTTTTG CTGCCCGCAA ACGGGCTGTT CTGGTGTTGC TAGTTTGTTA TCAGAATCGC
     3361 AGATCCGGCT TCAGCCGGTT TGCCGGCTGA AAGCGCTATT TCTTCCAGAA TTGCCATGAT
     3421 TTTTTCCCCA CGGGAGGCGT CACTGGCTCC CGTGTTGTCG GCAGCTGAGA GCGTTCACCG
     3481 ACAAACAACA GATAAAACGA AAGGCCCAGT CTTTCGACTG AGCCTTTCGT TTTATTTGAT
     3541 GCCTCTAGAG CTTGCATGCC TGCAGGTCTG GACATTTATT TGTATAGTTC ATCCATGCCA
     3601 TGTGTAATCC CAGCAGCTGT TACAAACTCA AGAAGGACCA TGTGGTCTCT CTTTTCGTTG
     3661 GGATCTTTCG AAAGGGCAGA TTGATAGGAC AGGTAATGGT TGTCTGGTAA AAGGACAGGG
     3721 CCATCGCCAA TTGGAGTATT TTGTTGATAA TGGTCTGCTA GTTGAACGCT TCCATCTTCA
     3781 ATGTTGTGTC TAATTTTGAA GTTAACTTTG ATTCCATTCT TTTGTTTGTC TGCCATGATG
     3841 TATACATTGT GTGAGTTATA GTTGTATTCC AATTTGTGTC CAAGAATGTT TCCATCTTCT
     3901 TTAAAATCAA TACCTTTTAA CTCGATTCTA TTAACAAGGG TATCACCTTC AAACTTGACT
     3961 TCAGCACGTG TCTTGTAGTT CCCGTCATCT TTGAAAAATA TAGTTCTTTC CTGTACATAA
     4021 CCTTCGGGCA TGGCACTCTT GAAAAAGTCA TGCTGTTTCA TATGATCTGG GTATCTCGCA
     4081 AAGCATTGAA GACCATAACC GAAAGTAGTG ACAAGTGTTG GCCATGGAAC AGGTAGTTTT
     4141 CCAGTAGTGC AAATAAATTT AAGGGTAAGT TTTCCGTATG TTGCATCACC TTCACCCTCT
     4201 CCACTGACAG AAAATTTGTG CCCATTAACA TCACCATCTA ATTCAACAAG AATTGGGACA
     4261 ACTCCAGTGA AAAGTTCTTC TCCTTTACTC ATATGTATAT CTCCTTCTTA AATCTAGAGG
     4321 ATCTCTCGAG aggtcaccgc cattaaaata gcgatattca ccattaacca tggtgagaat
     4381 atatttatgt cttgcatacg caattagaca attcccatgt agtgattgca tagttgactt
     4441 aatattacat aaacatatta ctgtttatta atgtagcacg ccgccctctt aagtcaaatt
     4501 gattgataaa taaatttaaa cgaagcggga gaacaggatg ataatgtgtg atgttctacg
     4561 ggcaggatga ctggatttat aatacaaaaa cgtgtttaag aacacaggga aattaatagt
     4621 aagcaaataa tcccttttcg tgacattaaa ggtaatcgct acatttaata aacattcata
     4681 taacatatat cttatcaaca cgatgaatag acagccaata tattattgcg attaataagc
     4741 aaccgaatgc ccagctgttt ttttaaaggc tgggcattcg gtttttacaa cgttatgtta
     4801 tcaggtgtgt ttaaagctgt tctgctgggc aatACCCTGC AGTTTCGGGT GATCGCTGAG
     4861 ATATTTCAGG GAGGCTTTGT AGTCTTCCAG CAGGATCCAT CGAGGTGTTG ATTCGATAAG
     4921 CAGCATCGCC TGTTTCAGGC TGTCTATGTG TGACTGTTGA GCTGTAACAA GTTGTCTCAG
     4981 GTGTTCAATT TCATGTTCTA GTTGCTTTGT TTTACTGGTT TCACCTGTTC TATTAGGTGT
     5041 TACATGCTGT TCATCTGTTA CATTGTCGAT CTGTTCATGG TGAACAGCTT TGAATGCACC
     5101 AAAAACTCGT AAAAGCTCTG ATGTATCTAT CTTTTTTACA CCGTTTTCAT CTGTGCATAT
     5161 GGACAGTTTT CCCTTTGATA TGTAACGGTG AACAGTTGTT CTACTTTTGT TTGTTAGTCT
     5221 TGATGCTTCA CTGATAGATA CAAGAGCCAT AAGAACCTCA GATCCTTCCG TATTTAGCCA
     5281 GTATGTTCTC TAGTGTGGTT CGTTGTTTTT GCGTGAGCCA TGAGAACGAA CCATTGAGAT
     5341 CATACTTACT TTGCATGTCA CTCAAAAATT TTGCCTCAAA ACTGGTGAGC TGAATTTTTG
     5401 CAGTTAAAGC ATCGTGTAGT GTTTTTCTTA GTCCGTTATG TAGGTAGGAA TCTGATGTAA
     5461 TGGTTGTTGG TATTTTGTCA CCATTCATTT TTATCTGGTT GTTCTCAAGT TCGGTTACGA
     5521 GATCCATTTG TCTATCTAGT TCAACTTGGA AAATCAACGT ATCAGTCGGG CGGCCTCGCT
     5581 TATCAACCAC CAATTTCATA TTGCTGTAAG TGTTTAAATC TTTACTTATT GGTTTCAAAA
     5641 CCCATTGGTT AAGCCTTTTA AACTCATGGT AGTTATTTTC AAGCATTAAC ATGAACTTAA
     5701 ATTCATCAAG GCTAATCTCT ATATTTGCCT TGTGAGTTTT CTTTTGTGTT AGTTCTTTTA
     5761 ATAACCACTC ATAAATCCTC ATAGAGTATT TGTTTTCAAA AGACTTAACA TGTTCCAGAT
     5821 TATATTTTAT GAATTTTTTT AACTGGAAAA GATAAGGCAA TATCTCTTCA CTAAAAACTA
     5881 ATTCTAATTT TTCGCTTGAG AACTTGGCAT AGTTTGTCCA CTGGAAAATC TCAAAGCCTT
     5941 TAACCAAAGG ATTCCTGATT TCCACAGTTC TCGTCATCAG CTCTCTGGTT GCTTTAGCTA
     6001 ATACACCATA AGCATTTTCC CTACTGATGT TCATCATCTG AGCGTATTGG TTATAAGTGA
     6061 ACGATACCGT CCGTTCTTTC CTTGTAGGGT TTTCAATCGT GGGGTTGAGT AGTGCCACAC
     6121 AGCATAAAAT TAGCTTGGTT TCATGCTCCG TTAAGTCATA GCGACTAATC GCTAGTTCAT
     6181 TTGCTTTGAA AACAACTAAT TCAGACATAC ATCTCAATTG GTCTAGGTGA TTTTAATCAC
     6241 TATACCAATT GAGATGGGCT AGTCAATGAT AATTACTAGT CCTTTTCCT
//