LOCUS       UA_gadX_CFP_const_YFP   5994 bp ds-DNA     circular     03-OCT-2024
DEFINITION  .
COMMENT     Circularized  GFP_mut_2
FEATURES             Location/Qualifiers
     misc_feature    675..780
                     /label="T0"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(806..1600)
                     /label="KanR"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          1728..1747
                     /label="UAXX_upProm_seqF"
                     /note="sequence: GGCAATTCCGACGTCTAAGA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1817..1882
                     /label="gadX_prom"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1883..2385
                     /label="gadX_prom"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    2398..2423
                     /label="RBS"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     gene            2424..3155
                     /label="cfp"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    3185..3264
                     /label="terminator BBa B0010"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          3387..3406
                     /label="UXX_2ins_seqF"
                     /note="sequence: CTGAAAGCGCTATTTCTTCC"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(3387..3406)
                     /label="UXX_1ins_seqR"
                     /note="sequence: GGAAGAAATAGCGCTTTCAG"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(3467..3546)
                     /label="terminator BBa B0010"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(3576..4292)
                     /label="YFP"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    4293..4318
                     /label="RBS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(4331..4401)
                     /label="const_W6_promoter"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(4350..4350)
                     /label=+1
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(4358..4363)
                     /label=-10
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(4381..4386)
                     /label=-35
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(4672..4691)
                     /label="UXX_2ins_seqR"
                     /note="sequence: GAACACCTGAGACAACTTGT"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    4963..5913
                     /label="pSC101"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
ORIGIN
        1 TTGAGTTGTG GGTATCTGTA AATTCTGCTA GACCTTTGCT GGAAAACTTG TAAATTCTGC
       61 TAGACCCTCT GTAAATTCCG CTAGACCTTT GTGTGTTTTT TTTGTTTATA TTCAAGTGGT
      121 TATAATTTAT AGAATAAAGA AAGAATAAAA AAAGATAAAA AGAATAGATC CCAGCCCTGT
      181 GTATAACTCA CTACTTTAGT CAGTTCCGCA GTATTACAAA AGGATGTCGC AAACGCTGTT
      241 TGCTCCTCTA CAAAACAGAC CTTAAAACCC TAAAGGCTTA AGTAGCGCCG TCGCAAGCTC
      301 CGGCAAATCG CTGAATATTC CTTTTGTCTC CGACCATCAG GCACCTGAGT CGCTGTCTTT
      361 TTCGTCACAT TCAGTTCGCT GCGCTCACGG CTCTGGCAGT GAATGGGGGT AAATGGCACT
      421 ACAGGCGCCT TTTATGGATT CATGCAAGGA AACTACCCAT AATACAAGAA AAGCCCGTCA
      481 CGGGCTTCTC AGGGCGTTTT ATGGCGGGTC TGCTATGTGG TGCTATCTGA CTTTTTGCTG
      541 TTCAGCAGTT CCTGCCCTCT GATTTTCCAG TCTGACCACT TCGGATTATC CCGTGACAGG
      601 TCATTCAGAC TGGCTAATGC ACCCAGTAAG GCAGCGGTAT CATCAACAGG CTTACCCGTC
      661 TTACTGTCCC TAGTGCTTGG ATTCTCACCA ATAAAAAACG CCCGGCGGCA ACCGAGCGTT
      721 CTGAACAAAT CCAGATGGAG TTCTGAGGTC ATTACTGGAT CTATCAACAG GAGTCCAAGC
      781 GAGCTCTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA
      841 TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC
      901 CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG CGGTCCGCCA
      961 CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG
     1021 GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG CGCGCCTTGA
     1081 GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT
     1141 CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT
     1201 CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG
     1261 ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA
     1321 ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC
     1381 CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCCTG CAGTTCATTC AGGGCACCGG
     1441 ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG
     1501 CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG
     1561 CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG
     1621 TCTCTTGATC AGATCTTGAT CCCCTGCGCC ATCAGATCCT TGGCGGCAAG AAAGCCATCC
     1681 AGTTTACTTT GCAGGGCTTC CCAACCTTAC CAGAGGGCGC CCCAGCTGGC AATTCCGACG
     1741 TCTAAGAAAC CATTATTATC ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT
     1801 TTCGTCTTCA CCTCGATGGA TCCTGCTGGA AGACTACAAA GCCTCCCTGA AATATCTCAG
     1861 CGATCACCCG AAACTGCAGG GTattgccca gcagaacagc tttaaacaca cctgataaca
     1921 taacgttgta aaaaccgaat gcccagcctt taaaaaaaca gctgggcatt cggttgctta
     1981 ttaatcgcaa taatatattg gctgtctatt catcgtgttg ataagatata tgttatatga
     2041 atgtttatta aatgtagcga ttacctttaa tgtcacgaaa agggattatt tgcttactat
     2101 taatttccct gtgttcttaa acacgttttt gtattataaa tccagtcatc ctgcccgtag
     2161 aacatcacac attatcatcc tgttctcccg cttcgtttaa atttatttat caatcaattt
     2221 gacttaagag ggcggcgtgc tacattaata aacagtaata tgtttatgta atattaagtc
     2281 aactatgcaa tcactacatg ggaattgtct aattgcgtat gcaagacata aatatattct
     2341 caccatggtt aatggtgaat atcgctattt taatggcggt gacctCTCGA GAGATCCTCT
     2401 AGATTTAAGA AGGAGATATA CATatgacta gcaaaagaag caaaggtgaa gaactgttca
     2461 ctggtgttgt tccaattctg gttgaactgg atggtgatgt taatggtcac aaattttctg
     2521 tctctggtga gggtgaaggt gatgcaacct acggtaaact gaccctgaaa tttatttgca
     2581 ctactggtaa actgcctgtt ccgtggccaa ccctggtcac tactctgact tggggtgttc
     2641 aatgctttgc tcgttaccca gatcacatga aacagcatga ctttttcaag tctgccatgc
     2701 cggaaggtta tgttcaggaa cgtactatct ttttcaaaga tgacggtaac tacaagaccc
     2761 gtgctgaagt caagtttgaa ggtgataccc tggttaatcg tatcgagctg aaaggtattg
     2821 attttaaaga agatggtaac attctgggtc acaaactgga atacaacgct atttctgata
     2881 atgtatacat cactgctgac aaacaaaaga atggtatcaa agctaatttc aaaattcgtc
     2941 acaacattga agatggtagc gttcaactgg cagaccatta tcaacaaaat actccaattg
     3001 gcgatggccc tgtcctgctg ccagacaacc attacctgtc cacccaatct cgtctgtcta
     3061 aagatccgaa cgaaaagcgc gatcacatgg tcctgctgga gtttgtaacc gctgctggta
     3121 ttaccctggg catggatgaa ctgtataaat aatagATGTC CAGACCTGCA GGCATGCAAG
     3181 CTCTAGAGGC ATCAAATAAA ACGAAAGGCT CAGTCGAAAG ACTGGGCCTT TCGTTTTATC
     3241 TGTTGTTTGT CGGTGAACGC TCTCCTGAGT AGGACAAATC CGCCGCCCTA GACCTAGGGT
     3301 ACGGGTTTTG CTGCCCGCAA ACGGGCTGTT CTGGTGTTGC TAGTTTGTTA TCAGAATCGC
     3361 AGATCCGGCT TCAGCCGGTT TGCCGGCTGA AAGCGCTATT TCTTCCAGAA TTGCCATGAT
     3421 TTTTTCCCCA CGGGAGGCGT CACTGGCTCC CGTGTTGTCG GCAGCTGAGA GCGTTCACCG
     3481 ACAAACAACA GATAAAACGA AAGGCCCAGT CTTTCGACTG AGCCTTTCGT TTTATTTGAT
     3541 GCCTCTAGAG CTTGCATGCC TGCAGGTCTG GACATTTATT TGTATAGTTC ATCCATGCCA
     3601 TGTGTAATCC CAGCAGCTGT TACAAACTCA AGAAGGACCA TGTGGTCTCT CTTTTCGTTG
     3661 GGATCTTTCG AAAGGGCAGA TTGATAGGAC AGGTAATGGT TGTCTGGTAA AAGGACAGGG
     3721 CCATCGCCAA TTGGAGTATT TTGTTGATAA TGGTCTGCTA GTTGAACGCT TCCATCTTCA
     3781 ATGTTGTGTC TAATTTTGAA GTTAACTTTG ATTCCATTCT TTTGTTTGTC TGCCATGATG
     3841 TATACATTGT GTGAGTTATA GTTGTATTCC AATTTGTGTC CAAGAATGTT TCCATCTTCT
     3901 TTAAAATCAA TACCTTTTAA CTCGATTCTA TTAACAAGGG TATCACCTTC AAACTTGACT
     3961 TCAGCACGTG TCTTGTAGTT CCCGTCATCT TTGAAAAATA TAGTTCTTTC CTGTACATAA
     4021 CCTTCGGGCA TGGCACTCTT GAAAAAGTCA TGCTGTTTCA TATGATCTGG GTATCTCGCA
     4081 AAGCATTGAA GACCATAACC GAAAGTAGTG ACAAGTGTTG GCCATGGAAC AGGTAGTTTT
     4141 CCAGTAGTGC AAATAAATTT AAGGGTAAGT TTTCCGTATG TTGCATCACC TTCACCCTCT
     4201 CCACTGACAG AAAATTTGTG CCCATTAACA TCACCATCTA ATTCAACAAG AATTGGGACA
     4261 ACTCCAGTGA AAAGTTCTTC TCCTTTACTC ATATGTATAT CTCCTTCTTA AATCTAGAGG
     4321 ATCTCTCGAG TTCGCCGTGT CCCTCTCGAT GGCTGTAATT TTCCTATAGG TTAGACTTTA
     4381 AGACAATACT CTTTTTGATA ACTCGATCCT CTACGCCGGA CGCATCGTGG CCGGCATCAC
     4441 CGGCGCCACA GGTGCGGTTG CTGGCGCCTA TATCGCCGAC ATCACCGATG GGGAAGATCG
     4501 GGCTCGCCAC TTCGGGCTCA TGAGCGCTTG TTTCGGCGTG GGTATGGTGG CAGGCCCCGT
     4561 GGCCGGGGGA CTGTTGGGCG CCATCTCCTT GCATGCGAAG ACGAAAGGGC CTTTGATTCG
     4621 ATAAGCAGCA TCGCCTGTTT CAGGCTGTCT ATGTGTGACT GTTGAGCTGT AACAAGTTGT
     4681 CTCAGGTGTT CAATTTCATG TTCTAGTTGC TTTGTTTTAC TGGTTTCACC TGTTCTATTA
     4741 GGTGTTACAT GCTGTTCATC TGTTACATTG TCGATCTGTT CATGGTGAAC AGCTTTGAAT
     4801 GCACCAAAAA CTCGTAAAAG CTCTGATGTA TCTATCTTTT TTACACCGTT TTCATCTGTG
     4861 CATATGGACA GTTTTCCCTT TGATATGTAA CGGTGAACAG TTGTTCTACT TTTGTTTGTT
     4921 AGTCTTGATG CTTCACTGAT AGATACAAGA GCCATAAGAA CCTCAGATCC TTCCGTATTT
     4981 AGCCAGTATG TTCTCTAGTG TGGTTCGTTG TTTTTGCGTG AGCCATGAGA ACGAACCATT
     5041 GAGATCATAC TTACTTTGCA TGTCACTCAA AAATTTTGCC TCAAAACTGG TGAGCTGAAT
     5101 TTTTGCAGTT AAAGCATCGT GTAGTGTTTT TCTTAGTCCG TTATGTAGGT AGGAATCTGA
     5161 TGTAATGGTT GTTGGTATTT TGTCACCATT CATTTTTATC TGGTTGTTCT CAAGTTCGGT
     5221 TACGAGATCC ATTTGTCTAT CTAGTTCAAC TTGGAAAATC AACGTATCAG TCGGGCGGCC
     5281 TCGCTTATCA ACCACCAATT TCATATTGCT GTAAGTGTTT AAATCTTTAC TTATTGGTTT
     5341 CAAAACCCAT TGGTTAAGCC TTTTAAACTC ATGGTAGTTA TTTTCAAGCA TTAACATGAA
     5401 CTTAAATTCA TCAAGGCTAA TCTCTATATT TGCCTTGTGA GTTTTCTTTT GTGTTAGTTC
     5461 TTTTAATAAC CACTCATAAA TCCTCATAGA GTATTTGTTT TCAAAAGACT TAACATGTTC
     5521 CAGATTATAT TTTATGAATT TTTTTAACTG GAAAAGATAA GGCAATATCT CTTCACTAAA
     5581 AACTAATTCT AATTTTTCGC TTGAGAACTT GGCATAGTTT GTCCACTGGA AAATCTCAAA
     5641 GCCTTTAACC AAAGGATTCC TGATTTCCAC AGTTCTCGTC ATCAGCTCTC TGGTTGCTTT
     5701 AGCTAATACA CCATAAGCAT TTTCCCTACT GATGTTCATC ATCTGAGCGT ATTGGTTATA
     5761 AGTGAACGAT ACCGTCCGTT CTTTCCTTGT AGGGTTTTCA ATCGTGGGGT TGAGTAGTGC
     5821 CACACAGCAT AAAATTAGCT TGGTTTCATG CTCCGTTAAG TCATAGCGAC TAATCGCTAG
     5881 TTCATTTGCT TTGAAAACAA CTAATTCAGA CATACATCTC AATTGGTCTA GGTGATTTTA
     5941 ATCACTATAC CAATTGAGAT GGGCTAGTCA ATGATAATTA CTAGTCCTTT TCCT
//