LOCUS       UA_recA_CFP_const_YFP   5636 bp ds-DNA     circular     03-OCT-2024
DEFINITION  .
COMMENT     Circularized  GFP_mut_2
FEATURES             Location/Qualifiers
     misc_feature    675..780
                     /label="T0"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(806..1600)
                     /label="KanR"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1824..2033
                     /label="recA_prom"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    2040..2065
                     /label="RBS"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     gene            2066..2797
                     /label="cfp"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    2827..2906
                     /label="terminator BBa B0010"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(3109..3188)
                     /label="terminator BBa B0010"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(3218..3934)
                     /label="YFP"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    3935..3960
                     /label="RBS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(3973..4043)
                     /label="const_W6_promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    complement(3992..3992)
                     /label=+1
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(4000..4005)
                     /label=-10
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(4023..4028)
                     /label=-35
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    4605..5555
                     /label="pSC101"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
ORIGIN
        1 TTGAGTTGTG GGTATCTGTA AATTCTGCTA GACCTTTGCT GGAAAACTTG TAAATTCTGC
       61 TAGACCCTCT GTAAATTCCG CTAGACCTTT GTGTGTTTTT TTTGTTTATA TTCAAGTGGT
      121 TATAATTTAT AGAATAAAGA AAGAATAAAA AAAGATAAAA AGAATAGATC CCAGCCCTGT
      181 GTATAACTCA CTACTTTAGT CAGTTCCGCA GTATTACAAA AGGATGTCGC AAACGCTGTT
      241 TGCTCCTCTA CAAAACAGAC CTTAAAACCC TAAAGGCTTA AGTAGCGCCG TCGCAAGCTC
      301 CGGCAAATCG CTGAATATTC CTTTTGTCTC CGACCATCAG GCACCTGAGT CGCTGTCTTT
      361 TTCGTCACAT TCAGTTCGCT GCGCTCACGG CTCTGGCAGT GAATGGGGGT AAATGGCACT
      421 ACAGGCGCCT TTTATGGATT CATGCAAGGA AACTACCCAT AATACAAGAA AAGCCCGTCA
      481 CGGGCTTCTC AGGGCGTTTT ATGGCGGGTC TGCTATGTGG TGCTATCTGA CTTTTTGCTG
      541 TTCAGCAGTT CCTGCCCTCT GATTTTCCAG TCTGACCACT TCGGATTATC CCGTGACAGG
      601 TCATTCAGAC TGGCTAATGC ACCCAGTAAG GCAGCGGTAT CATCAACAGG CTTACCCGTC
      661 TTACTGTCCC TAGTGCTTGG ATTCTCACCA ATAAAAAACG CCCGGCGGCA ACCGAGCGTT
      721 CTGAACAAAT CCAGATGGAG TTCTGAGGTC ATTACTGGAT CTATCAACAG GAGTCCAAGC
      781 GAGCTCTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA
      841 TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC
      901 CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG CGGTCCGCCA
      961 CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG
     1021 GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG CGCGCCTTGA
     1081 GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT
     1141 CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT
     1201 CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG
     1261 ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA
     1321 ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC
     1381 CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCCTG CAGTTCATTC AGGGCACCGG
     1441 ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG
     1501 CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG
     1561 CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG
     1621 TCTCTTGATC AGATCTTGAT CCCCTGCGCC ATCAGATCCT TGGCGGCAAG AAAGCCATCC
     1681 AGTTTACTTT GCAGGGCTTC CCAACCTTAC CAGAGGGCGC CCCAGCTGGC AATTCCGACG
     1741 TCTAAGAAAC CATTATTATC ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT
     1801 TTCGTCTTCA CCTCGATGGA TCCttgcaga ccttgtggca acaatttcta caaaacactt
     1861 gatactgtat gagcatacag tataattgct tcaacagaac atattgacta tccggtatta
     1921 cccggcatga caggagtaaa aatggctatc gacgaaaaca aacagaaagc gttggcggca
     1981 gcactgggcc agattgagaa acaatttggt aaaggctcca tcatgcgCTC GAGAGATCCT
     2041 CTAGATTTAA GAAGGAGATA TACATatgac tagcaaaaga agcaaaggtg aagaactgtt
     2101 cactggtgtt gttccaattc tggttgaact ggatggtgat gttaatggtc acaaattttc
     2161 tgtctctggt gagggtgaag gtgatgcaac ctacggtaaa ctgaccctga aatttatttg
     2221 cactactggt aaactgcctg ttccgtggcc aaccctggtc actactctga cttggggtgt
     2281 tcaatgcttt gctcgttacc cagatcacat gaaacagcat gactttttca agtctgccat
     2341 gccggaaggt tatgttcagg aacgtactat ctttttcaaa gatgacggta actacaagac
     2401 ccgtgctgaa gtcaagtttg aaggtgatac cctggttaat cgtatcgagc tgaaaggtat
     2461 tgattttaaa gaagatggta acattctggg tcacaaactg gaatacaacg ctatttctga
     2521 taatgtatac atcactgctg acaaacaaaa gaatggtatc aaagctaatt tcaaaattcg
     2581 tcacaacatt gaagatggta gcgttcaact ggcagaccat tatcaacaaa atactccaat
     2641 tggcgatggc cctgtcctgc tgccagacaa ccattacctg tccacccaat ctcgtctgtc
     2701 taaagatccg aacgaaaagc gcgatcacat ggtcctgctg gagtttgtaa ccgctgctgg
     2761 tattaccctg ggcatggatg aactgtataa ataatagATG TCCAGACCTG CAGGCATGCA
     2821 AGCTCTAGAG GCATCAAATA AAACGAAAGG CTCAGTCGAA AGACTGGGCC TTTCGTTTTA
     2881 TCTGTTGTTT GTCGGTGAAC GCTCTCCTGA GTAGGACAAA TCCGCCGCCC TAGACCTAGG
     2941 GTACGGGTTT TGCTGCCCGC AAACGGGCTG TTCTGGTGTT GCTAGTTTGT TATCAGAATC
     3001 GCAGATCCGG CTTCAGCCGG TTTGCCGGCT GAAAGCGCTA TTTCTTCCAG AATTGCCATG
     3061 ATTTTTTCCC CACGGGAGGC GTCACTGGCT CCCGTGTTGT CGGCAGCTGA GAGCGTTCAC
     3121 CGACAAACAA CAGATAAAAC GAAAGGCCCA GTCTTTCGAC TGAGCCTTTC GTTTTATTTG
     3181 ATGCCTCTAG AGCTTGCATG CCTGCAGGTC TGGACATTTA TTTGTATAGT TCATCCATGC
     3241 CATGTGTAAT CCCAGCAGCT GTTACAAACT CAAGAAGGAC CATGTGGTCT CTCTTTTCGT
     3301 TGGGATCTTT CGAAAGGGCA GATTGATAGG ACAGGTAATG GTTGTCTGGT AAAAGGACAG
     3361 GGCCATCGCC AATTGGAGTA TTTTGTTGAT AATGGTCTGC TAGTTGAACG CTTCCATCTT
     3421 CAATGTTGTG TCTAATTTTG AAGTTAACTT TGATTCCATT CTTTTGTTTG TCTGCCATGA
     3481 TGTATACATT GTGTGAGTTA TAGTTGTATT CCAATTTGTG TCCAAGAATG TTTCCATCTT
     3541 CTTTAAAATC AATACCTTTT AACTCGATTC TATTAACAAG GGTATCACCT TCAAACTTGA
     3601 CTTCAGCACG TGTCTTGTAG TTCCCGTCAT CTTTGAAAAA TATAGTTCTT TCCTGTACAT
     3661 AACCTTCGGG CATGGCACTC TTGAAAAAGT CATGCTGTTT CATATGATCT GGGTATCTCG
     3721 CAAAGCATTG AAGACCATAA CCGAAAGTAG TGACAAGTGT TGGCCATGGA ACAGGTAGTT
     3781 TTCCAGTAGT GCAAATAAAT TTAAGGGTAA GTTTTCCGTA TGTTGCATCA CCTTCACCCT
     3841 CTCCACTGAC AGAAAATTTG TGCCCATTAA CATCACCATC TAATTCAACA AGAATTGGGA
     3901 CAACTCCAGT GAAAAGTTCT TCTCCTTTAC TCATATGTAT ATCTCCTTCT TAAATCTAGA
     3961 GGATCTCTCG AGTTCGCCGT GTCCCTCTCG ATGGCTGTAA TTTTCCTATA GGTTAGACTT
     4021 TAAGACAATA CTCTTTTTGA TAACTCGATC CTCTACGCCG GACGCATCGT GGCCGGCATC
     4081 ACCGGCGCCA CAGGTGCGGT TGCTGGCGCC TATATCGCCG ACATCACCGA TGGGGAAGAT
     4141 CGGGCTCGCC ACTTCGGGCT CATGAGCGCT TGTTTCGGCG TGGGTATGGT GGCAGGCCCC
     4201 GTGGCCGGGG GACTGTTGGG CGCCATCTCC TTGCATGCGA AGACGAAAGG GCCTTTGATT
     4261 CGATAAGCAG CATCGCCTGT TTCAGGCTGT CTATGTGTGA CTGTTGAGCT GTAACAAGTT
     4321 GTCTCAGGTG TTCAATTTCA TGTTCTAGTT GCTTTGTTTT ACTGGTTTCA CCTGTTCTAT
     4381 TAGGTGTTAC ATGCTGTTCA TCTGTTACAT TGTCGATCTG TTCATGGTGA ACAGCTTTGA
     4441 ATGCACCAAA AACTCGTAAA AGCTCTGATG TATCTATCTT TTTTACACCG TTTTCATCTG
     4501 TGCATATGGA CAGTTTTCCC TTTGATATGT AACGGTGAAC AGTTGTTCTA CTTTTGTTTG
     4561 TTAGTCTTGA TGCTTCACTG ATAGATACAA GAGCCATAAG AACCTCAGAT CCTTCCGTAT
     4621 TTAGCCAGTA TGTTCTCTAG TGTGGTTCGT TGTTTTTGCG TGAGCCATGA GAACGAACCA
     4681 TTGAGATCAT ACTTACTTTG CATGTCACTC AAAAATTTTG CCTCAAAACT GGTGAGCTGA
     4741 ATTTTTGCAG TTAAAGCATC GTGTAGTGTT TTTCTTAGTC CGTTATGTAG GTAGGAATCT
     4801 GATGTAATGG TTGTTGGTAT TTTGTCACCA TTCATTTTTA TCTGGTTGTT CTCAAGTTCG
     4861 GTTACGAGAT CCATTTGTCT ATCTAGTTCA ACTTGGAAAA TCAACGTATC AGTCGGGCGG
     4921 CCTCGCTTAT CAACCACCAA TTTCATATTG CTGTAAGTGT TTAAATCTTT ACTTATTGGT
     4981 TTCAAAACCC ATTGGTTAAG CCTTTTAAAC TCATGGTAGT TATTTTCAAG CATTAACATG
     5041 AACTTAAATT CATCAAGGCT AATCTCTATA TTTGCCTTGT GAGTTTTCTT TTGTGTTAGT
     5101 TCTTTTAATA ACCACTCATA AATCCTCATA GAGTATTTGT TTTCAAAAGA CTTAACATGT
     5161 TCCAGATTAT ATTTTATGAA TTTTTTTAAC TGGAAAAGAT AAGGCAATAT CTCTTCACTA
     5221 AAAACTAATT CTAATTTTTC GCTTGAGAAC TTGGCATAGT TTGTCCACTG GAAAATCTCA
     5281 AAGCCTTTAA CCAAAGGATT CCTGATTTCC ACAGTTCTCG TCATCAGCTC TCTGGTTGCT
     5341 TTAGCTAATA CACCATAAGC ATTTTCCCTA CTGATGTTCA TCATCTGAGC GTATTGGTTA
     5401 TAAGTGAACG ATACCGTCCG TTCTTTCCTT GTAGGGTTTT CAATCGTGGG GTTGAGTAGT
     5461 GCCACACAGC ATAAAATTAG CTTGGTTTCA TGCTCCGTTA AGTCATAGCG ACTAATCGCT
     5521 AGTTCATTTG CTTTGAAAAC AACTAATTCA GACATACATC TCAATTGGTC TAGGTGATTT
     5581 TAATCACTAT ACCAATTGAG ATGGGCTAGT CAATGATAAT TACTAGTCCT TTTCCT
//