LOCUS       UA_const_CFP_recA_YFP   5621 bp ds-DNA     circular     03-OCT-2024
DEFINITION  .
COMMENT     Circularized  GFP_mut_2
FEATURES             Location/Qualifiers
     misc_feature    1409..1514
                     /label="T0"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(1540..2334)
                     /label="KanR"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     promoter        2739..2809
                     /label="const_W6_promoter"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     unsure          2754..2759
                     /label="-35 site"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     unsure          2777..2782
                     /label="-10 site"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     unsure          2790..2790
                     /label=+1
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    2825..2850
                     /label="RBS"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     gene            2851..3579
                     /label="cfp"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    3609..3688
                     /label="terminator BBa BB0010"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(3891..3970)
                     /label="terminator BBa B0010"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(4000..4716)
                     /label="YFP"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    4717..4742
                     /label="RBS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(4749..4958)
                     /label="recA promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    5324..653
                     /label="pSC101"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
ORIGIN
        1 AACGTATCAG TCGGGCGGCC TCGCTTATCA ACCACCAATT TCATATTGCT GTAAGTGTTT
       61 AAATCTTTAC TTATTGGTTT CAAAACCCAT TGGTTAAGCC TTTTAAACTC ATGGTAGTTA
      121 TTTTCAAGCA TTAACATGAA CTTAAATTCA TCAAGGCTAA TCTCTATATT TGCCTTGTGA
      181 GTTTTCTTTT GTGTTAGTTC TTTTAATAAC CACTCATAAA TCCTCATAGA GTATTTGTTT
      241 TCAAAAGACT TAACATGTTC CAGATTATAT TTTATGAATT TTTTTAACTG GAAAAGATAA
      301 GGCAATATCT CTTCACTAAA AACTAATTCT AATTTTTCGC TTGAGAACTT GGCATAGTTT
      361 GTCCACTGGA AAATCTCAAA GCCTTTAACC AAAGGATTCC TGATTTCCAC AGTTCTCGTC
      421 ATCAGCTCTC TGGTTGCTTT AGCTAATACA CCATAAGCAT TTTCCCTACT GATGTTCATC
      481 ATCTGAGCGT ATTGGTTATA AGTGAACGAT ACCGTCCGTT CTTTCCTTGT AGGGTTTTCA
      541 ATCGTGGGGT TGAGTAGTGC CACACAGCAT AAAATTAGCT TGGTTTCATG CTCCGTTAAG
      601 TCATAGCGAC TAATCGCTAG TTCATTTGCT TTGAAAACAA CTAATTCAGA CATACATCTC
      661 AATTGGTCTA GGTGATTTTA ATCACTATAC CAATTGAGAT GGGCTAGTCA ATGATAATTA
      721 CTAGTCCTTT TCCTTTGAGT TGTGGGTATC TGTAAATTCT GCTAGACCTT TGCTGGAAAA
      781 CTTGTAAATT CTGCTAGACC CTCTGTAAAT TCCGCTAGAC CTTTGTGTGT TTTTTTTGTT
      841 TATATTCAAG TGGTTATAAT TTATAGAATA AAGAAAGAAT AAAAAAAGAT AAAAAGAATA
      901 GATCCCAGCC CTGTGTATAA CTCACTACTT TAGTCAGTTC CGCAGTATTA CAAAAGGATG
      961 TCGCAAACGC TGTTTGCTCC TCTACAAAAC AGACCTTAAA ACCCTAAAGG CTTAAGTAGC
     1021 GCCGTCGCAA GCTCCGGCAA ATCGCTGAAT ATTCCTTTTG TCTCCGACCA TCAGGCACCT
     1081 GAGTCGCTGT CTTTTTCGTC ACATTCAGTT CGCTGCGCTC ACGGCTCTGG CAGTGAATGG
     1141 GGGTAAATGG CACTACAGGC GCCTTTTATG GATTCATGCA AGGAAACTAC CCATAATACA
     1201 AGAAAAGCCC GTCACGGGCT TCTCAGGGCG TTTTATGGCG GGTCTGCTAT GTGGTGCTAT
     1261 CTGACTTTTT GCTGTTCAGC AGTTCCTGCC CTCTGATTTT CCAGTCTGAC CACTTCGGAT
     1321 TATCCCGTGA CAGGTCATTC AGACTGGCTA ATGCACCCAG TAAGGCAGCG GTATCATCAA
     1381 CAGGCTTACC CGTCTTACTG TCCCTAGTGC TTGGATTCTC ACCAATAAAA AACGCCCGGC
     1441 GGCAACCGAG CGTTCTGAAC AAATCCAGAT GGAGTTCTGA GGTCATTACT GGATCTATCA
     1501 ACAGGAGTCC AAGCGAGCTC TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG
     1561 GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG
     1621 GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG
     1681 ATAGCGGTCC GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC
     1741 CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG
     1801 CATGCGCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC
     1861 CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG
     1921 TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC
     1981 ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC
     2041 CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC
     2101 TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CCTGCAGTTC
     2161 ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG
     2221 CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG
     2281 CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA
     2341 CGATCCTCAT CCTGTCTCTT GATCAGATCT TGATCCCCTG CGCCATCAGA TCCTTGGCGG
     2401 CAAGAAAGCC ATCCAGTTTA CTTTGCAGGG CTTCCCAACC TTACCAGAGG GCGCCCCAGC
     2461 TGGCAATTCC GACGTCTAAG AAACCATTAT TATCATGACA TTAACCTATA AAAATAGGCG
     2521 TATCACGAGG CCCTTTCGTC TTCGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA
     2581 CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC
     2641 GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG
     2701 TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGTT ATCAAAAAGA GTATTGTCTT
     2761 AAAGTCTAAC CTATAGGAAA ATTACAGCCA TCGAGAGGGA CACGGCGAAA CCTCGAGGGG
     2821 ATCCTCTAGA TTTAAGAAGG AGATATACAT atgactagca aaagaagcaa aggtgaagaa
     2881 ctgttcactg gtgttgttcc aattctggtt gaactggatg gtgatgttaa tggtcacaaa
     2941 ttttctgtct ctggtgaggg tgaaggtgat gcaacctacg gtaaactgac cctgaaattt
     3001 atttgcacta ctggtaaact gcctgttccg tggccaaccc tggtcactac tctgacttgg
     3061 ggtgttcaat gctttgctcg ttacccagat cacatgaaac agcatgactt tttcaagtct
     3121 gccatgccgg aaggttatgt tcaggaacgt actatctttt tcaaagatga cggtaactac
     3181 aagacccgtg ctgaagtcaa gtttgaaggt gataccctgg ttaatcgtat cgagctgaaa
     3241 ggtattgatt ttaaagaaga tggtaacatt ctgggtcaca aactggaata caacgctatt
     3301 tctgataatg tatacatcac tgctgacaaa caaaagaatg gtatcaaagc taatttcaaa
     3361 attcgtcaca acattgaaga tggtagcgtt caactggcag accattatca acaaaatact
     3421 ccaattggcg atggccctgt cctgctgcca gacaaccatt acctgtccac ccaatctcgt
     3481 ctgtctaaag atccgaacga aaagcgcgat cacatggtcc tgctggagtt tgtaaccgct
     3541 gctggtatta ccctgggcat ggatgaactg tataaataaA TGTCCAGACC TGCAGGCATG
     3601 CAAGCTCTAG AGGCATCAAA TAAAACGAAA GGCTCAGTCG AAAGACTGGG CCTTTCGTTT
     3661 TATCTGTTGT TTGTCGGTGA ACGCTCTCCT GAGTAGGACA AATCCGCCGC CCTAGACCTA
     3721 GGGTACGGGT TTTGCTGCCC GCAAACGGGC TGTTCTGGTG TTGCTAGTTT GTTATCAGAA
     3781 TCGCAGATCC GGCTTCAGCC GGTTTGCCGG CTGAAAGCGC TATTTCTTCC AGAATTGCCA
     3841 TGATTTTTTC CCCACGGGAG GCGTCACTGG CTCCCGTGTT GTCGGCAGCT GAGAGCGTTC
     3901 ACCGACAAAC AACAGATAAA ACGAAAGGCC CAGTCTTTCG ACTGAGCCTT TCGTTTTATT
     3961 TGATGCCTCT AGAGCTTGCA TGCCTGCAGG TCTGGACATT TATTTGTATA GTTCATCCAT
     4021 GCCATGTGTA ATCCCAGCAG CTGTTACAAA CTCAAGAAGG ACCATGTGGT CTCTCTTTTC
     4081 GTTGGGATCT TTCGAAAGGG CAGATTGATA GGACAGGTAA TGGTTGTCTG GTAAAAGGAC
     4141 AGGGCCATCG CCAATTGGAG TATTTTGTTG ATAATGGTCT GCTAGTTGAA CGCTTCCATC
     4201 TTCAATGTTG TGTCTAATTT TGAAGTTAAC TTTGATTCCA TTCTTTTGTT TGTCTGCCAT
     4261 GATGTATACA TTGTGTGAGT TATAGTTGTA TTCCAATTTG TGTCCAAGAA TGTTTCCATC
     4321 TTCTTTAAAA TCAATACCTT TTAACTCGAT TCTATTAACA AGGGTATCAC CTTCAAACTT
     4381 GACTTCAGCA CGTGTCTTGT AGTTCCCGTC ATCTTTGAAA AATATAGTTC TTTCCTGTAC
     4441 ATAACCTTCG GGCATGGCAC TCTTGAAAAA GTCATGCTGT TTCATATGAT CTGGGTATCT
     4501 CGCAAAGCAT TGAAGACCAT AACCGAAAGT AGTGACAAGT GTTGGCCATG GAACAGGTAG
     4561 TTTTCCAGTA GTGCAAATAA ATTTAAGGGT AAGTTTTCCG TATGTTGCAT CACCTTCACC
     4621 CTCTCCACTG ACAGAAAATT TGTGCCCATT AACATCACCA TCTAATTCAA CAAGAATTGG
     4681 GACAACTCCA GTGAAAAGTT CTTCTCCTTT ACTCATATGT ATATCTCCTT CTTAAATCTA
     4741 GAGGATCTCT CGAGcgcatg atggagcctt taccaaattg tttctcaatc tggcccagtg
     4801 ctgccgccaa cgctttctgt ttgttttcgt cgatagccat ttttactcct gtcatgccgg
     4861 gtaataccgg atagtcaata tgttctgttg aagcaattat actgtatgct catacagtat
     4921 caagtgtttt gtagaaattg ttgccacaag gtctgcaaGG ATCCATCGAG GTGTTGATTC
     4981 GATAAGCAGC ATCGCCTGTT TCAGGCTGTC TATGTGTGAC TGTTGAGCTG TAACAAGTTG
     5041 TCTCAGGTGT TCAATTTCAT GTTCTAGTTG CTTTGTTTTA CTGGTTTCAC CTGTTCTATT
     5101 AGGTGTTACA TGCTGTTCAT CTGTTACATT GTCGATCTGT TCATGGTGAA CAGCTTTGAA
     5161 TGCACCAAAA ACTCGTAAAA GCTCTGATGT ATCTATCTTT TTTACACCGT TTTCATCTGT
     5221 GCATATGGAC AGTTTTCCCT TTGATATGTA ACGGTGAACA GTTGTTCTAC TTTTGTTTGT
     5281 TAGTCTTGAT GCTTCACTGA TAGATACAAG AGCCATAAGA ACCTCAGATC CTTCCGTATT
     5341 TAGCCAGTAT GTTCTCTAGT GTGGTTCGTT GTTTTTGCGT GAGCCATGAG AACGAACCAT
     5401 TGAGATCATA CTTACTTTGC ATGTCACTCA AAAATTTTGC CTCAAAACTG GTGAGCTGAA
     5461 TTTTTGCAGT TAAAGCATCG TGTAGTGTTT TTCTTAGTCC GTTATGTAGG TAGGAATCTG
     5521 ATGTAATGGT TGTTGGTATT TTGTCACCAT TCATTTTTAT CTGGTTGTTC TCAAGTTCGG
     5581 TTACGAGATC CATTTGTCTA TCTAGTTCAA CTTGGAAAAT C
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