LOCUS       pAGD289                 6665 bp    DNA     circular UNA 01-FEB-2024
DEFINITION  Kva1-rpoB donor 90.
ACCESSION
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct.
FEATURES             Location/Qualifiers
     regulatory      118..136
                     /note="Geneious type: promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /standard_name="T7 promoter"
     protein_bind    complement(137..161)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /standard_name="lac operator"
     primer_bind     137..162
                     /standard_name="AGP939"
     primer_bind     complement(156..179)
                     /standard_name="AGP623"
     misc_feature    complement(168..179)
                     /standard_name="vector backbone"
     misc_feature    180..194
                     /note="Geneious type: motif"
                     /standard_name="fixed for synthesis"
     misc_feature    181..186
                     /note="Geneious type: RBS"
                     /note="ribosome binding site"
                     /standard_name="ribosome binding site RBS"
     CDS             195..1151
                     /standard_name="Kva1-RT"
     CDS             195..1151
                     /product="hypothetical protein"
                     /standard_name="(RT) CXOY01000010"
     misc_feature    198..200
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TCC"
     misc_feature    201..203
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TAT"
     misc_feature    204..206
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    213..215
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    216..218
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AGC"
     misc_feature    219..221
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAG"
     misc_feature    222..224
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ACT"
     misc_feature    225..227
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    228..230
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAC"
     misc_feature    234..236
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ACT"
     misc_feature    237..239
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CCG"
     misc_feature    243..245
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ACT"
     misc_feature    246..248
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATT"
     misc_feature    249..251
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    258..260
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TAC"
     misc_feature    261..263
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    264..266
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAG"
     misc_feature    270..272
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CCT"
     misc_feature    273..275
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAG"
     misc_feature    282..284
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAG"
     misc_feature    285..287
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GTG"
     misc_feature    294..296
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    297..299
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAA"
     misc_feature    300..302
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAG"
     misc_feature    306..308
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAC"
     misc_feature    318..320
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTC"
     misc_feature    321..323
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CGT"
     misc_feature    324..326
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAG"
     misc_feature    327..329
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    330..332
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GCG"
     misc_feature    333..335
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CAG"
     misc_feature    336..338
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CCG"
     misc_feature    339..341
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TCT"
     misc_feature    342..344
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CCG"
     misc_feature    345..347
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAC"
     misc_feature    351..353
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAG"
     misc_feature    354..356
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAA"
     misc_feature    366..368
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GCT"
     misc_feature    369..371
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATT"
     misc_feature    372..374
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GTT"
     misc_feature    375..377
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAC"
     misc_feature    378..380
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AGC"
     misc_feature    381..383
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTC"
     misc_feature    384..386
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    387..389
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TCT"
     misc_feature    390..392
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTC"
     misc_feature    393..395
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    396..398
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CCG"
     misc_feature    399..401
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GTT"
     misc_feature    402..404
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CAC"
     misc_feature    405..407
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAG"
     misc_feature    411..413
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GCG"
     misc_feature    414..416
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ACC"
     misc_feature    417..419
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GCG"
     misc_feature    426..428
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAG"
     misc_feature    435..437
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TCC"
     misc_feature    444..446
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAT"
     misc_feature    447..449
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAC"
     misc_feature    450..452
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GCA"
     misc_feature    459..461
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CAC"
     misc_feature    462..464
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAG"
     misc_feature    480..482
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTC"
     misc_feature    483..485
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAA"
     misc_feature    492..494
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TTC"
     misc_feature    495..497
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAA"
     misc_feature    501..503
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TTT"
     misc_feature    504..506
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TTC"
     misc_feature    507..509
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TAC"
     misc_feature    510..512
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AGC"
     misc_feature    513..515
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    519..521
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CCG"
     misc_feature    522..524
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CAG"
     misc_feature    525..527
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAT"
     misc_feature    528..530
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    531..533
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAC"
     misc_feature    540..542
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    543..545
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AGC"
     misc_feature    549..551
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAC"
     misc_feature    555..557
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATT"
     misc_feature    561..563
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    564..566
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAC"
     misc_feature    567..569
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CAA"
     misc_feature    582..584
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GTA"
     misc_feature    588..590
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AGC"
     misc_feature    591..593
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CAC"
     misc_feature    594..596
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TAC"
     misc_feature    597..599
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    603..605
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CGT"
     misc_feature    606..608
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CGC"
     misc_feature    609..611
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAG"
     misc_feature    612..614
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAA"
     misc_feature    615..617
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GGT"
     misc_feature    621..623
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAG"
     misc_feature    627..629
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    630..632
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CGC"
     misc_feature    633..635
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    639..641
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    645..647
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GCG"
     misc_feature    648..650
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CCG"
     misc_feature    651..653
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AGC"
     misc_feature    654..656
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TCT"
     misc_feature    657..659
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CCG"
     misc_feature    663..665
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    666..668
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TCC"
     misc_feature    669..671
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAC"
     misc_feature    672..674
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    675..677
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GTT"
     misc_feature    681..683
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TAC"
     misc_feature    684..686
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAC"
     misc_feature    687..689
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TTC"
     misc_feature    690..692
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAC"
     misc_feature    693..695
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAA"
     misc_feature    699..701
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    702..704
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TTC"
     misc_feature    714..716
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAA"
     misc_feature    720..722
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAC"
     misc_feature    726..728
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GTG"
     misc_feature    729..731
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    732..734
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TAC"
     misc_feature    735..737
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ACT"
     misc_feature    738..740
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CGT"
     misc_feature    741..743
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TAC"
     misc_feature    744..746
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GCT"
     misc_feature    750..752
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAC"
     misc_feature    753..755
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    762..764
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TCT"
     misc_feature    765..767
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TCC"
     misc_feature    768..770
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAT"
     misc_feature    771..773
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TCT"
     misc_feature    777..779
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAC"
     misc_feature    783..785
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    786..788
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAC"
     misc_feature    792..794
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GTC"
     misc_feature    795..797
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAA"
     misc_feature    798..800
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAC"
     misc_feature    801..803
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TTC"
     misc_feature    804..806
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GTT"
     misc_feature    810..812
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAC"
     misc_feature    813..815
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    822..824
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    825..827
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CAG"
     misc_feature    828..830
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAG"
     misc_feature    840..842
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    843..845
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATT"
     misc_feature    852..854
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ACT"
     misc_feature    855..857
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TCT"
     misc_feature    861..863
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ACC"
     misc_feature    864..866
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAA"
     misc_feature    867..869
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TTT"
     misc_feature    870..872
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATT"
     misc_feature    879..881
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GGT"
     misc_feature    882..884
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CGT"
     misc_feature    885..887
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AGC"
     misc_feature    894..896
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATT"
     misc_feature    897..899
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ACT"
     misc_feature    903..905
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GTG"
     misc_feature    915..917
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAC"
     misc_feature    918..920
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ACC"
     misc_feature    921..923
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GGC"
     misc_feature    927..929
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    930..932
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GGT"
     misc_feature    933..935
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GTG"
     misc_feature    936..938
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GGC"
     misc_feature    939..941
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CGT"
     misc_feature    942..944
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TTT"
     misc_feature    945..947
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    948..950
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CGT"
     misc_feature    951..953
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAG"
     misc_feature    957..959
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    960..962
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CGC"
     misc_feature    966..968
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    972..974
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TAC"
     misc_feature    975..977
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    978..980
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TAC"
     misc_feature    981..983
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AGC"
     misc_feature    1002..1004
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAG"
     misc_feature    1008..1010
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATT"
     misc_feature    1011..1013
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CCG"
     misc_feature    1014..1016
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TAC"
     misc_feature    1017..1019
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTC"
     misc_feature    1020..1022
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    1023..1025
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GGC"
     misc_feature    1035..1037
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CAC"
     misc_feature    1038..1040
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    1044..1046
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAT"
     misc_feature    1053..1055
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CCG"
     misc_feature    1056..1058
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAC"
     misc_feature    1062..1064
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TAC"
     misc_feature    1065..1067
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TAT"
     misc_feature    1068..1070
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AGC"
     misc_feature    1071..1073
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    1074..1076
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATT"
     misc_feature    1083..1085
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAC"
     misc_feature    1086..1088
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GGT"
     misc_feature    1098..1100
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    1107..1109
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    1113..1115
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAG"
     misc_feature    1119..1121
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AGC"
     misc_feature    1122..1124
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TTC"
     misc_feature    1125..1127
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GCA"
     misc_feature    1128..1130
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GTC"
     misc_feature    1131..1133
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    1149..1151
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TAA"
     primer_bind     complement(1155..1176)
                     /standard_name="AGP239"
     regulatory      1159..1164
                     /standard_name="NdeI site"
     regulatory      complement(1173..1179)
                     /standard_name="AarI/PaqCI site"
     gene            1182..1478
                     /standard_name="odd ncRNA?"
     sig_peptide     1314..1321
                     /standard_name="Stem"
     primer_bind     complement(1322..1411)
                     /standard_name="rpoB_editor"
     sig_peptide     complement(1412..1419)
                     /standard_name="Stem"
     regulatory      1481..1487
                     /standard_name="AarI/PaqCI site"
     primer_bind     complement(1485..1502)
                     /standard_name="AGP940"
     misc_feature    1495..1506
                     /standard_name="vector backbone"
     regulatory      1495..1502
                     /standard_name="NotI site"
     primer_bind     1495..1511
                     /standard_name="AGP622"
     CDS             1510..1527
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /translation="HHHHHH"
                     /standard_name="6xHis"
     primer_bind     1583..1606
                     /standard_name="AGP240"
     regulatory      complement(1594..1641)
                     /note="Geneious type: terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
                     /standard_name="T7 terminator"
     rep_origin      1678..2133
     regulatory      2160..2264
                     /note="Geneious type: promoter"
                     /gene="bla"
                     /standard_name="AmpR promoter"
     CDS             2265..3125
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /standard_name="AmpR"
     rep_origin      3296..3884
     CDS             complement(4314..4505)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
                     /standard_name="rop"
     CDS             complement(5314..6396)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
                     /standard_name="lacI"
     regulatory      complement(6397..6474)
                     /note="Geneious type: promoter"
                     /gene="lacI"
                     /standard_name="lacI promoter"
ORIGIN      
        1 cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg
       61 gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaattaa
      121 tacgactcac tataggggaa ttgtgagcgg ataacaattc ccctctagga tccgaattcg
      181 aaggagatat acatatgtcc tatatcgatg agctgagcaa gactctgaac tgcactccga
      241 agactattat ctctggctac atcaaggcgc ctaagaaata caaggtgttc tttatcaaaa
      301 agaaaaacgg taaaggcctc cgtgagatcg cgcagccgtc tccggacgtt aagaaagttc
      361 aacgtgctat tgttaacagc ctcctgtctc tcctgccggt tcacgagtgc gcgaccgcgt
      421 atatcgagga aaaatccatt gttgataacg cagaaccgca caagaaaaac gaatacatcc
      481 tcaaaatgga tttcgaaaat tttttctaca gcatcaaacc gcaggatctg aactatttta
      541 tcagcaaaaa cgatattaaa ctgaaccaat ttgaatatga agtaattagc cactacctgt
      601 ttcgtcgcaa gaaaggtgaa aagaaactgc gcctgtgcat cggtgcgccg agctctccga
      661 tgatctccaa catcgttatg tacgacttcg acgaaaaact gttcaactac tgtaaagaaa
      721 acgatgtgat ctacactcgt tacgctgatg acctgtcttt ttcttccgat tcttttgaca
      781 aactgaacga tgtcaaaaac ttcgttatta acctggtgtc tctgcagaag aaaccagaac
      841 tgattatcaa cacttctaaa accaaattta ttggtaaagg tcgtagccgt cgcattactg
      901 gtgtggttat ttctaacacc ggcgatctgg gtgtgggccg ttttctgcgt aagaaaatcc
      961 gcgcactgtt ttacctgtac agcaataaca aaatcaatca taaggacatt ccgtacctcc
     1021 tgggcatgct gtctcacatc aaaaatatcg aaccggacta ttactatagc ctgattaaaa
     1081 ttaacggtga ggacaaactg aaacaactgg gtaaggatag cttcgcagtc ctgcagaaaa
     1141 acaatgacta ataccgttca tatgaggcta gagcaggtga tccataatct tgagacaagc
     1201 agcttgattg atgcaaatca ttaagttagt gtataacata ctctttacgc gatagaggcg
     1261 tatctgcttc atctggtgta tcaccggaac ttgttgattg tggtcattta tgccacggcc
     1321 tccgatttcc gcagcagtga aagagttctt cggttccagc cagctgcctc agtttatgga
     1381 ccagaacaac ccgctgtctg agattacgca caggccgtgc ccttctaggt aaagagcaga
     1441 accccggttt accggggttt tttataacat aagcggctgc cacctgcatg atccgcggcc
     1501 gcactcgagc accaccacca ccaccactga gatccggctg ctaacaaagc ccgaaaggaa
     1561 gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa
     1621 cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggattggc gaatgggacg
     1681 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     1741 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     1801 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     1861 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     1921 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     1981 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     2041 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     2101 cgaattttaa caaaatatta acgtttacaa tttcaggtgg cacttttcgg ggaaatgtgc
     2161 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     2221 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     2281 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     2341 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     2401 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     2461 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     2521 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     2581 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     2641 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     2701 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     2761 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa
     2821 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     2881 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     2941 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     3001 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     3061 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     3121 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     3181 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     3241 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     3301 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     3361 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     3421 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     3481 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     3541 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     3601 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     3661 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     3721 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     3781 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     3841 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     3901 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     3961 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     4021 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt
     4081 attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac
     4141 aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg
     4201 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     4261 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     4321 ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg
     4381 tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga
     4441 agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg
     4501 gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa
     4561 cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt
     4621 tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt
     4681 caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct
     4741 gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac
     4801 gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag
     4861 cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc
     4921 cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggggccgcc
     4981 atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag
     5041 gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg
     5101 ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg
     5161 agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac
     5221 cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgagatcc cggtgcctaa
     5281 tgagtgagct aacttacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     5341 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     5401 gggcgccagg gtggtttttc ttttcaccag tgagacgggc aacagctgat tgcccttcac
     5461 cgcctggccc tgagagagtt gcagcaagcg gtccacgctg gtttgcccca gcaggcgaaa
     5521 atcctgtttg atggtggtta acggcgggat ataacatgag ctgtcttcgg tatcgtcgta
     5581 tcccactacc gagatatccg caccaacgcg cagcccggac tcggtaatgg cgcgcattgc
     5641 gcccagcgcc atctgatcgt tggcaaccag catcgcagtg ggaacgatgc cctcattcag
     5701 catttgcatg gtttgttgaa aaccggacat ggcactccag tcgccttccc gttccgctat
     5761 cggctgaatt tgattgcgag tgagatattt atgccagcca gccagacgca gacgcgccga
     5821 gacagaactt aatgggcccg ctaacagcgc gatttgctgg tgacccaatg cgaccagatg
     5881 ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata atactgttga tgggtgtctg
     5941 gtcagagaca tcaagaaata acgccggaac attagtgcag gcagcttcca cagcaatggc
     6001 atcctggtca tccagcggat agttaatgat cagcccactg acgcgttgcg cgagaagatt
     6061 gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct accatcgaca ccaccacgct
     6121 ggcacccagt tgatcggcgc gagatttaat cgccgcgaca atttgcgacg gcgcgtgcag
     6181 ggccagactg gaggtggcaa cgccaatcag caacgactgt ttgcccgcca gttgttgtgc
     6241 cacgcggttg ggaatgtaat tcagctccgc catcgccgct tccacttttt cccgcgtttt
     6301 cgcagaaacg tggctggcct ggttcaccac gcgggaaacg gtctgataag agacaccggc
     6361 atactctgcg acatcgtata acgttactgg tttcacattc accaccctga attgactctc
     6421 ttccgggcgc tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg tgtccgggat
     6481 ctcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc
     6541 cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc
     6601 cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc
     6661 gagcc
//
