LOCUS       pAGK55                  6366 bp    DNA     circular UNA 19-JAN-2024
DEFINITION  Node6 + split guide + 10 bp insert.
ACCESSION   urn.local...e4-en0v1pq
VERSION     urn.local...e4-en0v1pq
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     regulatory      1..364
                     /note="Geneious type: promoter"
                     /gene="CAG Enhancer"
                     /standard_name="CAG enhancer"
     regulatory      365..646
                     /note="Geneious type: promoter"
                     /gene="CAG Promoter"
                     /standard_name="CAG promoter"
     regulatory      647..1525
                     /note="Geneious type: promoter"
                     /gene="CAG Intron"
                     /standard_name="CAG intron"
     misc_feature    1615..>1732
                     /gene="Multiple Cloning Site"
                     /standard_name="MCS"
     misc_feature    1733..1736
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    1733..3739
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Eco3_GG_insert (concatenated sequence 2)"
     misc_feature    1733..3735
                     /standard_name="INSERT_FOR_SYNTHESIS"
     misc_feature    <1733..1736
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="bbsi"
     misc_feature    1733..>1736
                     /standard_name="BbsI site"
     misc_feature    1739..2668
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
                     NNNNNNNNNNNNNNNNNNNNNNNNNNN"
     misc_feature    <1739..>2668
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Eco3_GG_insert (concatenated sequence 2)"
     misc_feature    <1739..>2668
                     /standard_name="INSERT_FOR_SYNTHESIS"
     misc_feature    1739..>2668
                     /standard_name="fig|1736225.3.peg.2969 "
     CDS             1739..>2668
                     /product="Retron-type RNA-directed DNA polymerase (EC
                     2.7.7.49)"
                     /standard_name="LMLK01000022"
     misc_feature    1742..1744
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AGC"
     misc_feature    1745..1747
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GTG"
     misc_feature    1763..1765
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GGT"
     misc_feature    1766..1768
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTC"
     misc_feature    1769..1771
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    1781..1783
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAG"
     misc_feature    1784..1786
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTT"
     misc_feature    1787..1789
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAT"
     misc_feature    1790..1792
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GTC"
     misc_feature    1793..1795
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GTT"
     misc_feature    1796..1798
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTT"
     misc_feature    1802..1804
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTC"
     misc_feature    1808..1810
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AGT"
     misc_feature    1811..1813
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GCT"
     misc_feature    1814..1816
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AGT"
     misc_feature    1817..1819
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CGC"
     misc_feature    1832..1834
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TAT"
     misc_feature    1835..1837
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAG"
     misc_feature    1841..1843
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CCA"
     misc_feature    1847..1849
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AGG"
     misc_feature    1850..1852
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ACT"
     misc_feature    1853..1855
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ACC"
     misc_feature    1856..1858
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GGC"
     misc_feature    1868..1870
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    1877..1879
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CCA"
     misc_feature    1883..1885
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAG"
     misc_feature    1886..1888
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GCT"
     misc_feature    1889..1891
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    1892..1894
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAG"
     misc_feature    1895..1897
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAT"
     misc_feature    1898..1900
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TAC"
     misc_feature    1904..1906
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CGC"
     misc_feature    1910..1912
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TTC"
     misc_feature    1913..1915
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    1919..1921
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TTG"
     misc_feature    1922..1924
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TAT"
     misc_feature    1925..1927
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AGG"
     misc_feature    1931..1933
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CCC"
     misc_feature    1934..1936
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GTC"
     misc_feature    1940..1942
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAG"
     misc_feature    1946..1948
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GCT"
     misc_feature    1952..1954
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GCA"
     misc_feature    1955..1957
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TAC"
     misc_feature    1964..1966
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GGT"
     misc_feature    1967..1969
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAG"
     misc_feature    1970..1972
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TCT"
     misc_feature    1976..1978
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AGG"
     misc_feature    1985..1987
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GCC"
     misc_feature    1991..1993
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GTC"
     misc_feature    1994..1996
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CAC"
     misc_feature    1997..1999
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GCC"
     misc_feature    2000..2002
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CAG"
     misc_feature    2003..2005
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAC"
     misc_feature    2006..2008
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TCC"
     misc_feature    2009..2011
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TTT"
     misc_feature    2012..2014
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTT"
     misc_feature    2015..2017
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    2018..2020
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAG"
     misc_feature    2024..2026
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAC"
     misc_feature    2030..2032
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAG"
     misc_feature    2033..2035
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAT"
     misc_feature    2039..2041
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TTC"
     misc_feature    2045..2047
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AGC"
     misc_feature    2048..2050
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    2051..2053
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ACC"
     misc_feature    2054..2056
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CCA"
     misc_feature    2057..2059
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAT"
     misc_feature    2063..2065
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TTC"
     misc_feature    2081..2083
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CGC"
     misc_feature    2090..2092
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AGT"
     misc_feature    2093..2095
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAC"
     misc_feature    2096..2098
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ACT"
     misc_feature    2099..2101
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CCC"
     misc_feature    2102..2104
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CAG"
     misc_feature    2108..2110
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TCT"
     misc_feature    2111..2113
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GCG"
     misc_feature    2126..2128
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TAC"
     misc_feature    2129..2131
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GTA"
     misc_feature    2135..2137
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CAT"
     misc_feature    2138..2140
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    2141..2143
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTC"
     misc_feature    2144..2146
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TTT"
     misc_feature    2150..2152
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CAA"
     misc_feature    2153..2155
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CCT"
     misc_feature    2156..2158
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GCC"
     misc_feature    2159..2161
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAG"
     misc_feature    2162..2164
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AGG"
     misc_feature    2168..2170
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAG"
     misc_feature    2174..2176
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    2177..2179
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    2180..2182
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTC"
     misc_feature    2183..2185
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AGC"
     misc_feature    2186..2188
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GTG"
     misc_feature    2192..2194
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GCT"
     misc_feature    2195..2197
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CCG"
     misc_feature    2198..2200
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AGT"
     misc_feature    2207..2209
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GTG"
     misc_feature    2210..2212
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GTC"
     misc_feature    2213..2215
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AGC"
     misc_feature    2216..2218
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAT"
     misc_feature    2219..2221
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TTC"
     misc_feature    2222..2224
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TGC"
     misc_feature    2225..2227
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    2231..2233
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAG"
     misc_feature    2234..2236
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TTC"
     misc_feature    2240..2242
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAT"
     misc_feature    2252..2254
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAG"
     misc_feature    2255..2257
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TTC"
     misc_feature    2258..2260
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TGC"
     misc_feature    2267..2269
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    2270..2272
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAC"
     misc_feature    2273..2275
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    2276..2278
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GCA"
     misc_feature    2282..2284
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ACC"
     misc_feature    2285..2287
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CGT"
     misc_feature    2288..2290
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TAC"
     misc_feature    2291..2293
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GCC"
     misc_feature    2303..2305
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ACA"
     misc_feature    2306..2308
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TTC"
     misc_feature    2309..2311
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TCA"
     misc_feature    2315..2317
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAC"
     misc_feature    2318..2320
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ACC"
     misc_feature    2321..2323
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AGG"
     misc_feature    2324..2326
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAC"
     misc_feature    2330..2332
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTC"
     misc_feature    2333..2335
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GGG"
     misc_feature    2336..2338
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GTT"
     misc_feature    2339..2341
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    2342..2344
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CCT"
     misc_feature    2345..2347
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    2348..2350
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    2351..2353
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATT"
     misc_feature    2357..2359
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAG"
     misc_feature    2360..2362
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    2363..2365
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TTG"
     misc_feature    2369..2371
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAG"
     misc_feature    2372..2374
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    2375..2377
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TAC"
     misc_feature    2378..2380
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CGT"
     misc_feature    2381..2383
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CGC"
     misc_feature    2393..2395
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    2396..2398
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAC"
     misc_feature    2399..2401
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CGC"
     misc_feature    2402..2404
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GCG"
     misc_feature    2405..2407
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAG"
     misc_feature    2411..2413
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GTC"
     misc_feature    2414..2416
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TTC"
     misc_feature    2417..2419
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TCA"
     misc_feature    2420..2422
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TCT"
     misc_feature    2426..2428
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GCT"
     misc_feature    2429..2431
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CAT"
     misc_feature    2435..2437
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CGC"
     misc_feature    2438..2440
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CAC"
     misc_feature    2441..2443
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GTC"
     misc_feature    2444..2446
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ACC"
     misc_feature    2453..2455
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ACT"
     misc_feature    2465..2467
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAG"
     misc_feature    2468..2470
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GGA"
     misc_feature    2471..2473
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAA"
     misc_feature    2474..2476
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    2477..2479
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AGT"
     misc_feature    2480..2482
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTT"
     misc_feature    2483..2485
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GGG"
     misc_feature    2486..2488
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CGT"
     misc_feature    2504..2506
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATT"
     misc_feature    2507..2509
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAG"
     misc_feature    2510..2512
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CAC"
     misc_feature    2525..2527
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TAC"
     misc_feature    2528..2530
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CGG"
     misc_feature    2531..2533
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TAC"
     misc_feature    2534..2536
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAT"
     misc_feature    2540..2542
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    2543..2545
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAC"
     misc_feature    2549..2551
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GCC"
     misc_feature    2552..2554
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAT"
     misc_feature    2555..2557
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAG"
     misc_feature    2558..2560
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GCG"
     misc_feature    2561..2563
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TAC"
     misc_feature    2567..2569
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GTT"
     misc_feature    2576..2578
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTC"
     misc_feature    2579..2581
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GCA"
     misc_feature    2582..2584
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TTC"
     misc_feature    2585..2587
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GCG"
     misc_feature    2591..2593
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CAC"
     misc_feature    2603..2605
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAG"
     misc_feature    2609..2611
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    2612..2614
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    2615..2617
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CGG"
     misc_feature    2624..2626
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CGC"
     misc_feature    2627..2629
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAA"
     misc_feature    2633..2635
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ACC"
     misc_feature    2642..2644
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTC"
     misc_feature    2648..2650
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAG"
     misc_feature    2651..2653
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CGC"
     misc_feature    2657..2659
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AGA"
     misc_feature    2660..2662
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CGT"
     misc_feature    2663..2665
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CAG"
     misc_feature    2666..2668
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CAG"
     CDS             2668
                     /product="hypothetical protein"
                     /standard_name="LMLK01000022"
     misc_feature    2669..2692
                     /note="Geneious type: tag"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SV40 NLS"
     primer_bind     2669..2695
                     /standard_name="AGK159"
     misc_feature    2720..2944
                     /note="Geneious type: polyA_signal"
                     /note="bovine growth hormone polyadenylation signal"
                     /standard_name="bGH poly(A) signal"
     misc_feature    2953..3761
                     /note="Geneious type: gblock"
                     /standard_name="gblock_AGK55"
     primer_bind     complement(2953..2978)
                     /standard_name="AGK149"
     misc_feature    2979..3496
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     regulatory      2979..3219
                     /note="Geneious type: promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="U6 promoter"
     misc_feature    <2979..>3219
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Eco3_GG_insert (concatenated sequence 2)"
     misc_feature    3195..>3219
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="for_430 (concatenated sequence 4)"
     misc_feature    <3220..>3223
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Eco3_GG_insert (concatenated sequence 2)"
     misc_feature    <3224..3228
                     /note="Geneious type: extention"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="long primer bases -- include to bring a1
                     up to 16 bases minimum (delete from left as necessary)"
     misc_feature    <3224..>3488
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Eco3_GG_insert (concatenated sequence 2)"
     misc_feature    <3224..>3488
                     /standard_name="INSERT_FOR_SYNTHESIS"
     gene            3229..3239
                     /standard_name="a1?"
     misc_feature    3229..>3319
                     /standard_name="ncRNA"
     misc_feature    3306..3319
                     /standard_name="msd Stem"
     CDS             <3320..>3449
                     /codon_start=3
                     /gene="EMX1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Gnomon."
                     /product="homeobox protein EMX1 isoform X2"
                     /protein_id="XP_011530999.1"
                     /db_xref="GeneID:2016"
                     /db_xref="HGNC:HGNC:3340"
                     /db_xref="MIM:600034"
     misc_feature    3320..3449
                     /note="Geneious type: donor"
                     /standard_name="editing donor"
     misc_feature    3352..3368
                     /note="Geneious type: gRNA"
                     /standard_name="misc"
     misc_feature    3369..3378
                     /note="Geneious type: umi"
                     /standard_name="UMI 161"
     misc_feature    3379
                     /note="Geneious type: mut"
                     /standard_name="G -> A"
     misc_feature    3379..3381
                     /note="Geneious type: gRNA"
                     /standard_name="misc"
     misc_feature    3382..3384
                     /note="Geneious type: pam"
                     /standard_name="PAM"
     misc_feature    3383..3384
                     /note="Geneious type: mut"
                     /standard_name="GG -> TT"
     misc_feature    <3450..3483
                     /standard_name="ncRNA"
     misc_feature    complement(3450..3463)
                     /standard_name="msd Stem"
     gene            complement(3473..3483)
                     /standard_name="a2?"
     misc_feature    complement(3484..>3488)
                     /note="Geneious type: extention"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="long primer bases -- include to bring a1
                     up to 16 bases minimum (delete from right as necessary)"
     misc_feature    <3489..>3496
                     /note="Geneious type: gblock"
     regulatory      3489..3496
                     /note="Geneious type: terminator"
                     /standard_name="Terminator"
     misc_feature    <3489..>3496
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Eco3_GG_insert (concatenated sequence 2)"
     misc_feature    <3489..>3496
                     /standard_name="INSERT_FOR_SYNTHESIS"
     regulatory      3497..3711
                     /note="Geneious type: promoter"
                     /note="human H1 RNA promoter"
                     /standard_name="H1 promoter"
     primer_bind     3632..3651
                     /note="Human H1 promoter, forward primer"
                     /standard_name="H1"
     misc_feature    3715^3716
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="TGATAAGATTCCGTAAGAGCCAAACCTAGCATTTTATGGGTT
                     AATAGCCCATCGGGCCATGAGTCATGGTTTCGCCTAGTATTTTAGCTATGCCCGTCGT
                     TCAGTTCGCTGAACAAACGGCAGAAGCTGGAGGAGGAAGGGCCTGAGTCCGAGCAGAA
                     GAATTACGTCTGCAAAGTTCTCCCATCACATCAACCGGTGGCGCATTGCCACGAAGCA
                     GGCCAATGGGGAGGACATCGATGTCATCAGCGAACTGATCGACGTGCTCAAGTAGGTT
                     TGGCTCTTACGGAATCTTATCA"
     misc_feature    3716..3735
                     /note="Geneious type: motif"
                     /standard_name="DON""T CHANGE"
     misc_feature    3716..3735
                     /note="Geneious type: gRNA"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="gRNA"
     gene            <3716..>3735
                     /gene="EMX1"
                     /note="empty spiracles homeobox 1; Derived by automated
                     computational analysis using gene prediction method:
                     BestRefSeq,Gnomon."
                     /db_xref="GeneID:2016"
                     /db_xref="HGNC:HGNC:3340"
                     /db_xref="MIM:600034"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="EMX1 gene"
     misc_feature    3736..3739
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    3736..1736
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pSCL273 (concatenated sequence 1)"
     misc_feature    3736..3826
                     /standard_name="modified SpCas9 scaffold"
     misc_feature    complement(3736..>3739)
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="bbsi"
     primer_bind     complement(3740..3764)
                     /standard_name="AGK089"
     regulatory      complement(3856..3977)
                     /note="Geneious type: promoter"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="pLac"
     primer_bind     complement(3864..3887)
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="M13-R (-46)"
     misc_feature    4054..4073
                     /note="Geneious type: oligo"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SV40Promoter(20mer)"
     rep_origin      4070..4205
     regulatory      4225..4346
                     /note="Geneious type: terminator"
                     /note="simian vacuolating virus 40 (SV40) polyA signal"
                     /regulatory_class="terminator"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SV40 polyA"
     gene            complement(5357..6217)
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="AmpR"
     regulatory      complement(6218..6322)
                     /note="Geneious type: promoter"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="AmpR promoter"
     regulatory      complement(6259..6287)
                     /note="Geneious type: Promoter"
                     /standard_name="amp prom"
ORIGIN      
        1 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
       61 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
      121 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
      181 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      241 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
      301 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      361 accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca
      421 cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg
      481 gggggggggg gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg
      541 aaaggtgcgg cggcagccaa tcaaagcggc gcgctccgaa agtttccttt tatggcgagg
      601 cggcggcggc ggcggcccta taaaaagcga accgcgcggc gggcgggagt cgctgcgcgc
      661 tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg
      721 accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattac
      781 cgcttggttt aatgacggct tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc
      841 cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg
      901 gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg
      961 gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg
     1021 tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga
     1081 gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc tgcacccccc tccccgagtt
     1141 gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg ggcgtggcgc ggggctcgcc
     1201 gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc
     1261 ggggagggct cgggggaggg gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg
     1321 gcgagccgca gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg
     1381 tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc
     1441 ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg
     1501 ccgcgccgcc gtccccttct ccctctccag cctcggggct gtccgcgggg ggacggctgc
     1561 cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg cggctctaga
     1621 gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg
     1681 gttattgtgc tgtctcatca ttttggcaaa gaattgagct cgatatcggt acgccaccat
     1741 gagcgtgatc aatgggctgg ctggtctcct gaatcagagc gagcttgatg tcgttcttta
     1801 cctccagagt gctagtcgca agtacaaggt gtataagatc ccaaagagga ctaccggctt
     1861 ccggattatc gcccagccag caaaggctct gaaggattac cagcgcgcct tcctgcagtt
     1921 gtataggctg cccgtccaca agtgtgctat ggcataccag aaaggtaagt ctgtgaggga
     1981 taacgccctg gtccacgccc agaactcctt tcttctgaag acagacctgg aggatttttt
     2041 caacagcatc accccagata ttttctggga gagcatcgag cgctccccaa gtgacactcc
     2101 ccagatctct gcggaggaca agggctacgt agagcatctg ctcttttggc aacctgccaa
     2161 gaggagcaag cgcctgatcc tcagcgtggg cgctccgagt tcccccgtgg tcagcaattt
     2221 ctgcctgtat gagttcgaca atctgatcag cgagttctgc ctgcctctga acatcgcata
     2281 cacccgttac gccgatgacc tgacattctc atgtaacacc agggacgtgc tcggggttct
     2341 gcctctgatc attgagaagc tgttgaacaa gctgtaccgt cgcaagctga ggctgaaccg
     2401 cgcgaagacc gtcttctcat ctatggctca taaccgccac gtcaccggtg ttactatcaa
     2461 caatgaggga gaactgagtc ttgggcgtga gcgtaaacgc ttcattaagc acctgattaa
     2521 tcagtaccgg tacgatatga tcgacgaggc cgataaggcg tacctggttg gcctgctcgc
     2581 attcgcgaaa cacatcgagc ctgagttcat cctgcggatg aaccgcaaat acaccactga
     2641 actcatggag cgcatcagac gtcagcagcc accaaagaag aaaagaaagg tctgatcccg
     2701 gttagtaatg agtttaaacc tgtgccttct agttgccagc catctgttgt ttgcccctcc
     2761 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     2821 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     2881 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     2941 atggagatct gcgaaatcgg atccagctga atctaagtga gggcctattt cccatgattc
     3001 cttcatattt gcatatacga tacaaggctg ttagagagat aattggaatt aatttgactg
     3061 taaacacaaa gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt
     3121 ttgcagtttt aaaattatgt tttaaaatgg actatcatat gcttaccgta acttgaaagt
     3181 atttcgattt cttggcttta tatatcttgt ggaaaggacg aaaccgtatc aactctttag
     3241 cgtggacgta ttacgtctag tcgggtgatt agccagactc taacttattg aacgcatttg
     3301 gggttgcgaa agtgtcgcaa caaacggcag aagctggagg aggaagggcc tgagtccgag
     3361 cagaagaact cgcagtagaa agttctccca tcacatcaac cggtggcgca ttgccacgaa
     3421 gcaggccaat ggggaggaca tcgatgtcat gcgacacttt cgcttcctcc agtaaagagt
     3481 tgatacggtt ttttttgaac gctgacgtca tcaacccgct ccaaggaatc gcgggcccag
     3541 tgtcactagg cgggaacacc cagcgcgcgt gcgccctggc aggaagatgg ctgtgaggga
     3601 caggggagtg gcgccctgca atatttgcat gtcgctatgt gttctgggaa atcaccataa
     3661 acgtgaaatg tctttggatt tgggaatctt ataagttctg tatgagacca cgaaagagtc
     3721 cgagcagaag aagaagtttc agagctatgc tggaaacagc atagcaagtt gaaataaggc
     3781 tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg cttttttttt tttagcttgg
     3841 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     3901 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     3961 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga
     4021 tccgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     4081 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     4141 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     4201 aggcctaggc ttttgcaaaa agctaacttg tttattgcag cttataatgg ttacaaataa
     4261 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     4321 ttgtccaaac tcatcaatgt atcttatcat gtctggatcc gctgcattaa tgaatcggcc
     4381 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     4441 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     4501 ggttatccac agaatcaggg gataacgcaa gaaagaacat gtgagcaaaa ggccagcaaa
     4561 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccatatgctc cgcccccctg
     4621 acgagcatca caaaaatcga cgctcaggtc ataggtggcg aaacccgaca ggactataaa
     4681 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttcca accctgccgc
     4741 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     4801 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     4861 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     4921 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     4981 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     5041 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     5101 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     5161 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     5221 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     5281 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     5341 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     5401 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     5461 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     5521 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     5581 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     5641 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     5701 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     5761 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     5821 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     5881 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     5941 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     6001 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     6061 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     6121 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     6181 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     6241 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     6301 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctggg tcgacattga
     6361 ttattg
//
