LOCUS       pAGK56                  6521 bp    DNA     circular UNA 19-JAN-2024
DEFINITION  Node412.1 + split guide + 10 bp insert.
ACCESSION   urn.local...e4-en0v1pq
VERSION     urn.local...e4-en0v1pq
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     regulatory      1..364
                     /note="Geneious type: promoter"
                     /gene="CAG Enhancer"
                     /standard_name="CAG enhancer"
     regulatory      365..646
                     /note="Geneious type: promoter"
                     /gene="CAG Promoter"
                     /standard_name="CAG promoter"
     regulatory      647..1525
                     /note="Geneious type: promoter"
                     /gene="CAG Intron"
                     /standard_name="CAG intron"
     misc_feature    1615..>1732
                     /gene="Multiple Cloning Site"
                     /standard_name="MCS"
     misc_feature    1733..1736
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    1733..3894
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Eco3_GG_insert (concatenated sequence 2)"
     misc_feature    1733..3890
                     /standard_name="INSERT_FOR_SYNTHESIS"
     misc_feature    <1733..1736
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="bbsi"
     misc_feature    1733..>1736
                     /standard_name="BbsI site"
     misc_feature    1739..2761
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
                     NNNNNNNNNNNNNNNNNNNNNNNNNNN"
     gene            1739..>2761
                     /product="Retron-type RNA-directed DNA polymerase (EC
                     2.7.7.49)"
                     /standard_name="(RT) CP003985"
     misc_feature    <1739..>2761
                     /standard_name="INSERT_FOR_SYNTHESIS"
     misc_feature    1748..1750
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAG"
     misc_feature    1754..1756
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TAC"
     misc_feature    1757..1759
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CGC"
     misc_feature    1760..1762
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAG"
     misc_feature    1763..1765
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAG"
     misc_feature    1766..1768
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TTT"
     misc_feature    1769..1771
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ACC"
     misc_feature    1772..1774
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    1775..1777
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CAG"
     misc_feature    1778..1780
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GCT"
     misc_feature    1781..1783
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ACC"
     misc_feature    1784..1786
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GCC"
     misc_feature    1787..1789
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAG"
     misc_feature    1790..1792
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TCT"
     misc_feature    1793..1795
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    1799..1801
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAG"
     misc_feature    1811..1813
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CAG"
     misc_feature    1814..1816
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CAC"
     misc_feature    1817..1819
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GCC"
     misc_feature    1823..1825
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AGT"
     misc_feature    1829..1831
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GCC"
     misc_feature    1832..1834
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CGC"
     misc_feature    1835..1837
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GGC"
     misc_feature    1841..1843
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TAC"
     misc_feature    1844..1846
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAG"
     misc_feature    1853..1855
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    1859..1861
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATT"
     misc_feature    1865..1867
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TTT"
     misc_feature    1868..1870
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TCC"
     misc_feature    1874..1876
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAC"
     misc_feature    1877..1879
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CAC"
     misc_feature    1880..1882
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    1883..1885
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AGC"
     misc_feature    1886..1888
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CGC"
     misc_feature    1889..1891
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTC"
     misc_feature    1892..1894
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GTG"
     misc_feature    1895..1897
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GGC"
     misc_feature    1901..1903
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAC"
     misc_feature    1904..1906
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    1907..1909
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAT"
     misc_feature    1910..1912
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TAC"
     misc_feature    1913..1915
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    1922..1924
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GCT"
     misc_feature    1928..1930
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TCC"
     misc_feature    1931..1933
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ACT"
     misc_feature    1934..1936
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAG"
     misc_feature    1937..1939
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TCC"
     misc_feature    1943..1945
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CAG"
     misc_feature    1949..1951
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TAT"
     misc_feature    1952..1954
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AGA"
     misc_feature    1955..1957
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TGC"
     misc_feature    1958..1960
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TAC"
     misc_feature    1961..1963
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAG"
     misc_feature    1964..1966
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATT"
     misc_feature    1967..1969
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAA"
     misc_feature    1970..1972
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAG"
     misc_feature    1979..1981
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TCC"
     misc_feature    1985..1987
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ACT"
     misc_feature    1994..1996
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ACT"
     misc_feature    1997..1999
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    2006..2008
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CCA"
     misc_feature    2009..2011
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    2012..2014
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CCG"
     misc_feature    2018..2020
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    2021..2023
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAG"
     misc_feature    2027..2029
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    2033..2035
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CGC"
     misc_feature    2039..2041
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    2042..2044
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    2045..2047
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAC"
     misc_feature    2051..2053
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    2054..2056
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    2060..2062
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CAG"
     misc_feature    2066..2068
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ACG"
     misc_feature    2069..2071
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    2072..2074
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TCC"
     misc_feature    2075..2077
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAG"
     misc_feature    2081..2083
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GCT"
     misc_feature    2087..2089
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GCC"
     misc_feature    2090..2092
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TAC"
     misc_feature    2093..2095
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GTC"
     misc_feature    2096..2098
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GTG"
     misc_feature    2105..2107
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TCT"
     misc_feature    2108..2110
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    2111..2113
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAG"
     misc_feature    2114..2116
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAC"
     misc_feature    2117..2119
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAC"
     misc_feature    2129..2131
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CAC"
     misc_feature    2132..2134
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    2135..2137
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CGC"
     misc_feature    2138..2140
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CAG"
     misc_feature    2147..2149
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GTC"
     misc_feature    2150..2152
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTC"
     misc_feature    2153..2155
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CGC"
     misc_feature    2156..2158
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    2162..2164
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GTC"
     misc_feature    2165..2167
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAG"
     misc_feature    2168..2170
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAC"
     misc_feature    2177..2179
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CCA"
     misc_feature    2180..2182
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AGC"
     misc_feature    2183..2185
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    2189..2191
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GGC"
     misc_feature    2192..2194
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CAG"
     misc_feature    2195..2197
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAC"
     misc_feature    2198..2200
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GTC"
     misc_feature    2204..2206
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CAC"
     misc_feature    2210..2212
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TTT"
     misc_feature    2216..2218
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAT"
     misc_feature    2219..2221
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATT"
     misc_feature    2222..2224
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GGC"
     misc_feature    2228..2230
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TCT"
     misc_feature    2237..2239
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GTG"
     misc_feature    2240..2242
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AGC"
     misc_feature    2249..2251
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    2252..2254
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ACC"
     misc_feature    2255..2257
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GGC"
     misc_feature    2258..2260
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    2261..2263
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ACG"
     misc_feature    2264..2266
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ACC"
     misc_feature    2267..2269
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    2270..2272
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAG"
     misc_feature    2279..2281
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTC"
     misc_feature    2282..2284
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CCC"
     misc_feature    2285..2287
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CAG"
     misc_feature    2291..2293
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GCG"
     misc_feature    2294..2296
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CCT"
     misc_feature    2300..2302
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TCC"
     misc_feature    2303..2305
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CCG"
     misc_feature    2306..2308
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ACT"
     misc_feature    2309..2311
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    2312..2314
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TCC"
     misc_feature    2315..2317
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAC"
     misc_feature    2318..2320
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    2321..2323
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TTT"
     misc_feature    2327..2329
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAC"
     misc_feature    2330..2332
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CGC"
     misc_feature    2336..2338
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAC"
     misc_feature    2339..2341
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GCC"
     misc_feature    2342..2344
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AGG"
     misc_feature    2345..2347
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATT"
     misc_feature    2348..2350
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GCC"
     misc_feature    2357..2359
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TGT"
     misc_feature    2366..2368
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AGG"
     misc_feature    2369..2371
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAG"
     misc_feature    2375..2377
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CGT"
     misc_feature    2384..2386
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CGC"
     misc_feature    2387..2398
                     /note="Geneious type: motif"
                     /standard_name="YSDD"
     misc_feature    2387..2389
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TAC"
     misc_feature    2390..2392
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AGC"
     misc_feature    2399..2401
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    2402..2404
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ACG"
     misc_feature    2408..2410
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AGC"
     misc_feature    2411..2413
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GGC"
     misc_feature    2414..2416
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAG"
     misc_feature    2417..2419
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TTC"
     misc_feature    2426..2428
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GGT"
     misc_feature    2429..2431
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAG"
     misc_feature    2432..2434
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    2435..2437
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    2441..2443
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TTC"
     misc_feature    2453..2455
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GTG"
     misc_feature    2459..2461
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAG"
     misc_feature    2465..2467
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TCT"
     misc_feature    2471..2473
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTT"
     misc_feature    2474..2476
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    2489..2491
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAG"
     misc_feature    2492..2494
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ACG"
     misc_feature    2495..2497
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAG"
     misc_feature    2498..2500
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CAG"
     misc_feature    2504..2506
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TTT"
     misc_feature    2507..2509
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAG"
     misc_feature    2513..2515
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CAG"
     misc_feature    2516..2518
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CGC"
     misc_feature    2522..2524
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TTC"
     misc_feature    2528..2530
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ACC"
     misc_feature    2534..2536
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    2537..2539
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GTC"
     misc_feature    2540..2542
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GTG"
     misc_feature    2546..2548
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAG"
     misc_feature    2549..2551
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AGG"
     misc_feature    2552..2554
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    2555..2557
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAC"
     misc_feature    2558..2560
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GTA"
     misc_feature    2564..2566
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CGC"
     misc_feature    2570..2572
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAG"
     misc_feature    2573..2575
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CGT"
     misc_feature    2576..2578
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CGC"
     misc_feature    2579..2581
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAG"
     misc_feature    2582..2584
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    2585..2587
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CGG"
     misc_feature    2588..2590
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CAG"
     misc_feature    2591..2593
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAG"
     misc_feature    2609..2611
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAG"
     misc_feature    2612..2614
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TAC"
     misc_feature    2615..2617
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GGC"
     misc_feature    2618..2620
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTC"
     misc_feature    2624..2626
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GGA"
     misc_feature    2630..2632
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    2633..2635
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATT"
     misc_feature    2636..2638
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AGG"
     misc_feature    2639..2641
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ACC"
     misc_feature    2642..2644
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAA"
     misc_feature    2645..2647
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAG"
     misc_feature    2651..2653
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CGC"
     misc_feature    2654..2656
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ACG"
     misc_feature    2663..2665
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    2669..2671
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CAC"
     misc_feature    2672..2674
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TTG"
     misc_feature    2678..2680
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GGC"
     misc_feature    2681..2683
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    2684..2686
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TCC"
     misc_feature    2687..2689
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAT"
     misc_feature    2690..2692
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TTC"
     misc_feature    2693..2695
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    2696..2698
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ACA"
     misc_feature    2699..2701
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CAC"
     misc_feature    2702..2704
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    2717..2719
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CGC"
     misc_feature    2720..2722
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAT"
     misc_feature    2723..2725
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GCA"
     misc_feature    2726..2728
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    2729..2731
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAG"
     misc_feature    2732..2734
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GCG"
     misc_feature    2735..2737
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAG"
     misc_feature    2741..2743
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    2744..2746
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTC"
     misc_feature    2747..2749
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AGG"
     misc_feature    2756..2758
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATT"
     misc_feature    2759..2761
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ACG"
     misc_feature    2762..2785
                     /note="Geneious type: tag"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SV40 NLS"
     misc_feature    2813..3037
                     /note="Geneious type: polyA_signal"
                     /note="bovine growth hormone polyadenylation signal"
                     /standard_name="bGH poly(A) signal"
     misc_feature    3046..3916
                     /note="Geneious type: gblock"
                     /standard_name="gblock56"
     misc_feature    3072..3651
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     misc_feature    <3072..>3312
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Eco3_GG_insert (concatenated sequence 2)"
     misc_feature    <3072..>3312
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="o269-o270 (concatenated sequence 3)"
     regulatory      3072..3312
                     /note="Geneious type: promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="U6 promoter"
     misc_feature    3288..>3312
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="for_430 (concatenated sequence 4)"
     misc_feature    <3313..>3316
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Eco3_GG_insert (concatenated sequence 2)"
     misc_feature    <3317..3321
                     /note="Geneious type: extention"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="long primer bases -- include to bring a1
                     up to 16 bases minimum (delete from left as necessary)"
     misc_feature    <3317..>3643
                     /standard_name="INSERT_FOR_SYNTHESIS"
     misc_feature    3322..3332
                     /note="Geneious type: motif"
                     /standard_name="a1?"
     misc_feature    3333
                     /note="Geneious type: motif"
                     /standard_name="pG?"
     misc_feature    3466..3473
                     /note="Geneious type: motif"
                     /standard_name="truncated stem (8bp)"
     CDS             <3474..>3603
                     /codon_start=3
                     /gene="EMX1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Gnomon."
                     /product="homeobox protein EMX1 isoform X2"
                     /protein_id="XP_011530999.1"
                     /db_xref="GeneID:2016"
                     /db_xref="HGNC:HGNC:3340"
                     /db_xref="MIM:600034"
     misc_feature    complement(<3474..>3603)
                     /note="Geneious type: msd"
                     /standard_name="Eco3 msd"
     misc_feature    3506..3522
                     /note="Geneious type: gRNA"
                     /standard_name="misc"
     misc_feature    3523..3532
                     /note="Geneious type: umi"
                     /standard_name="umi69"
     misc_feature    3533
                     /note="Geneious type: mut"
                     /standard_name="G -> A"
     misc_feature    3533..3535
                     /note="Geneious type: gRNA"
                     /standard_name="misc"
     misc_feature    3536..3538
                     /note="Geneious type: pam"
                     /standard_name="PAM"
     misc_feature    3537..3538
                     /note="Geneious type: mut"
                     /standard_name="GG -> TT"
     misc_feature    complement(3604..3611)
                     /note="Geneious type: motif"
                     /standard_name="truncated stem (8bp)"
     misc_feature    3628..3638
                     /note="Geneious type: motif"
                     /standard_name="a2?"
     misc_feature    complement(3639..>3643)
                     /note="Geneious type: extention"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="long primer bases -- include to bring a1
                     up to 16 bases minimum (delete from right as necessary)"
     misc_feature    <3644..>3651
                     /note="Geneious type: gblock"
     regulatory      3644..3651
                     /note="Geneious type: terminator"
                     /standard_name="Terminator"
     misc_feature    <3644..>3651
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Eco3_GG_insert (concatenated sequence 2)"
     misc_feature    <3644..>3651
                     /standard_name="INSERT_FOR_SYNTHESIS"
     regulatory      3652..3866
                     /note="Geneious type: promoter"
                     /note="human H1 RNA promoter"
                     /standard_name="H1 promoter"
     primer_bind     3787..3806
                     /note="Human H1 promoter, forward primer"
                     /standard_name="H1"
     misc_feature    3870^3871
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="TGATAAGATTCCGTAAGAGCCAAACCTAGCATTTTATGGGTT
                     AATAGCCCATCGGGCCATGAGTCATGGTTTCGCCTAGTATTTTAGCTATGCCCGTCGT
                     TCAGTTCGCTGAACAAACGGCAGAAGCTGGAGGAGGAAGGGCCTGAGTCCGAGCAGAA
                     GAATTACGTCTGCAAAGTTCTCCCATCACATCAACCGGTGGCGCATTGCCACGAAGCA
                     GGCCAATGGGGAGGACATCGATGTCATCAGCGAACTGATCGACGTGCTCAAGTAGGTT
                     TGGCTCTTACGGAATCTTATCA"
     misc_feature    3871..3890
                     /note="Geneious type: gRNA"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="gRNA"
     gene            <3871..>3890
                     /gene="EMX1"
                     /note="empty spiracles homeobox 1; Derived by automated
                     computational analysis using gene prediction method:
                     BestRefSeq,Gnomon."
                     /db_xref="GeneID:2016"
                     /db_xref="HGNC:HGNC:3340"
                     /db_xref="MIM:600034"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="EMX1 gene"
     misc_feature    3871..3890
                     /note="Geneious type: motif"
                     /standard_name="DON""T CHANGE"
     misc_feature    3891..3894
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    3891..1736
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pSCL273 (concatenated sequence 1)"
     misc_feature    3891..3981
                     /standard_name="modified SpCas9 scaffold"
     misc_feature    complement(3891..>3894)
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="bbsi"
     regulatory      complement(4011..4132)
                     /note="Geneious type: promoter"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="pLac"
     primer_bind     complement(4019..4042)
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="M13-R (-46)"
     misc_feature    4209..4228
                     /note="Geneious type: oligo"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SV40Promoter(20mer)"
     rep_origin      4225..4360
     regulatory      4380..4501
                     /note="Geneious type: terminator"
                     /note="simian vacuolating virus 40 (SV40) polyA signal"
                     /regulatory_class="terminator"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SV40 polyA"
     gene            complement(5512..6372)
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="AmpR"
     regulatory      complement(6373..6477)
                     /note="Geneious type: promoter"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="AmpR promoter"
     regulatory      complement(6414..6442)
                     /note="Geneious type: Promoter"
                     /standard_name="amp prom"
ORIGIN      
        1 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
       61 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
      121 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
      181 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      241 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
      301 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      361 accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca
      421 cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg
      481 gggggggggg gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg
      541 aaaggtgcgg cggcagccaa tcaaagcggc gcgctccgaa agtttccttt tatggcgagg
      601 cggcggcggc ggcggcccta taaaaagcga accgcgcggc gggcgggagt cgctgcgcgc
      661 tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg
      721 accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattac
      781 cgcttggttt aatgacggct tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc
      841 cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg
      901 gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg
      961 gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg
     1021 tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga
     1081 gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc tgcacccccc tccccgagtt
     1141 gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg ggcgtggcgc ggggctcgcc
     1201 gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc
     1261 ggggagggct cgggggaggg gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg
     1321 gcgagccgca gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg
     1381 tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc
     1441 ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg
     1501 ccgcgccgcc gtccccttct ccctctccag cctcggggct gtccgcgggg ggacggctgc
     1561 cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg cggctctaga
     1621 gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg
     1681 gttattgtgc tgtctcatca ttttggcaaa gaattgagct cgatatcggt acgccaccat
     1741 gaattggaag aaataccgca aggagtttac catccaggct accgccaagt ctctggatga
     1801 gaagagcatg cagcacgccc tgagttatgc ccgcggcttg tacaaggcaa acctgccaat
     1861 tatcttttcc cagaaccacc tgagccgcct cgtgggctat aacatcgatt acatccgcaa
     1921 cgcttcttcc actaagtccg atcagttcta tagatgctac aagattaaaa agaaatcatc
     1981 cgatactatg cgcactatct gtgagccact gccgagcctg aaggagatcc agcgctggat
     2041 cctggacaac atcctgagcc aggtcacgat ctccaagttt gctaaggcct acgtcgtggg
     2101 attttctatc aaggacaacg caaggtttca cctgcgccag aaacaagtcc tccgcatcga
     2161 tgtcaaggac ttctttccaa gcatcaaggg ccagaacgtc tttcacatct ttaaaaatat
     2221 tggctattct gctgaggtga gcgcaatgct gaccggcctg acgaccctga agaactgcct
     2281 cccccagggt gcgcctactt ccccgactct gtccaacatc tttatgaacc gctgcgacgc
     2341 caggattgcc ggctactgtc tggcaaggaa gattcgttac acccgctaca gcgacgatct
     2401 gacgttcagc ggcgagttcg atgtcggtaa gctgatctct ttcatcaaca tggtgtttaa
     2461 ggactctggc cttctgttga acaagaccaa gacgaagcag atgtttaagc atcagcgcca
     2521 gttcacaacc ggtctggtcg tgaacgagag gatcaacgta tgtcgcgaga agcgtcgcaa
     2581 gctgcggcag gagatttttt acatcaaaaa gtacggcctc gacggacacc tgattaggac
     2641 cgaagagaag cgcacgaatt acatcatgca cttgcttggc ctgtccaatt tcatcacaca
     2701 catcaacgct aacgaccgcg atgcactgga ggcgaaggaa atcctcagga aatatattac
     2761 gccaccaaag aagaaaagaa aggtctgatc ccggttagta atgagtttaa acctgtgcct
     2821 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     2881 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     2941 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     3001 aatagcaggc atgctgggga tgcggtgggc tctatggaga tctgcgaaat cggatccagc
     3061 tgaatctaag tgagggccta tttcccatga ttccttcata tttgcatata cgatacaagg
     3121 ctgttagaga gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata
     3181 cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa
     3241 tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct
     3301 tgtggaaagg acgaaaccgt acggcctcac tagccttagt ggcttagcca cggaatggcg
     3361 agtcgccatt ctggggagaa cttgctttca agcccaggcg aagattcacc aactcttgga
     3421 aagagttggc gaatcttcgc cccgcttccg caaaagcctc cagccccgag atgacaaacg
     3481 gcagaagctg gaggaggaag ggcctgagtc cgagcagaag aagctcaatc agaaagttct
     3541 cccatcacat caaccggtgg cgcattgcca cgaagcaggc caatggggag gacatcgatg
     3601 tcacatctcg gtgattatgt ccgtatgtag tgaggccgta cggttttttt tgaacgctga
     3661 cgtcatcaac ccgctccaag gaatcgcggg cccagtgtca ctaggcggga acacccagcg
     3721 cgcgtgcgcc ctggcaggaa gatggctgtg agggacaggg gagtggcgcc ctgcaatatt
     3781 tgcatgtcgc tatgtgttct gggaaatcac cataaacgtg aaatgtcttt ggatttggga
     3841 atcttataag ttctgtatga gaccacgaaa gagtccgagc agaagaagaa gtttcagagc
     3901 tatgctggaa acagcatagc aagttgaaat aaggctagtc cgttatcaac ttgaaaaagt
     3961 ggcaccgagt cggtgctttt ttttttttag cttggcgtaa tcatggtcat agctgtttcc
     4021 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     4081 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     4141 cgctttccag tcgggaaacc tgtcgtgcca gcggatccgc atctcaatta gtcagcaacc
     4201 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     4261 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     4321 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagcta
     4381 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     4441 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     4501 atcatgtctg gatccgctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     4561 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     4621 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     4681 cgcaagaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     4741 gttgctggcg tttttccata tgctccgccc ccctgacgag catcacaaaa atcgacgctc
     4801 aggtcatagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     4861 ctccctcgtg cgctctcctg ttccaaccct gccgcttacc ggatacctgt ccgcctttct
     4921 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     4981 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     5041 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     5101 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     5161 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     5221 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     5281 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     5341 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     5401 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     5461 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     5521 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     5581 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     5641 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     5701 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     5761 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     5821 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     5881 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     5941 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     6001 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     6061 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     6121 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     6181 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     6241 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     6301 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     6361 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     6421 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     6481 atttccccga aaagtgccac ctgggtcgac attgattatt g
//
