LOCUS       pMXs-IP-PEX3-EGF        7665 bp DNA     circular SYN 09-DEC-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7665)
  AUTHORS   Noboru Mizushima
  TITLE     Direct Submission
  JOURNAL   Exported Dec 13, 2024 from SnapGene 7.2.1
            https://www.snapgene.com
COMMENT     Sequence Label: pMXs-IP-PEX3-EGFP
FEATURES             Location/Qualifiers
     source          1..7665
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             22..615
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    678..1035
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1094..1510
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     misc_feature    1520..1894
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1823..1846
                     /label=Universal Primer 1
     misc_feature    1926..3047
                     /label=Pex3
     CDS             3080..3796
                     /codon_start=1
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3115..3134)
                     /label=Universal Primer 13
     primer_bind     3739..3758
                     /label=Universal Primer 12
     misc_feature    3833..4385
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3849..3872)
                     /label=Universal Primer 2
     primer_bind     4311..4334
                     /label=Universal Primer 3
     misc_feature    4394..4399
                     /label=SalI
     CDS             4400..4999
                     /codon_start=1
                     /label=PuroR
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4641..4665)
                     /label=Universal Primer 7
     misc_feature    5000..5005
                     /label=SalI
     primer_bind     complement(5018..5043)
                     /label=Universal Primer 4
     LTR             5170..5639
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     rep_origin      complement(5940..6528)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(6692..7552)
                     /label=AmpR
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
      541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
      601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
      661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
      721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
      781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
      841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
      901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct
      961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
     1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa
     1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
     1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
     1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
     1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
     1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
     1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
     1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa
     1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca
     1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca
     1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg
     1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac
     1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc
     1861 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aattaaggat
     1921 ctgccatgct gaggtctgta tggaattttc tgaaacgcca caaaaagaaa tgcatcttcc
     1981 tgggcacggt ccttggagga gtatatattc tggggaaata tggacagaag aaaatcagag
     2041 aaatacagga aagggaggct gcagaataca ttgcccaagc acgacgacaa tatcattttg
     2101 aaagtaacca gaggacttgc aatatgacag tgctgtccat gcttccaaca ctgagagagg
     2161 ccttaatgca gcaactgaat tccgagagcc tcacagctct gctaaaaaac aggccttcaa
     2221 acaagctaga aatatgggag gatctgaaga taataagttt cacaagaagt actgtggctg
     2281 tatacagtac ctgtatgctg gttgttcttt tgcgggtcca gttaaacata attggtggat
     2341 atatttacct ggataatgca gcagttggca aaaatggcac tacaattctt gctcccccag
     2401 atgtccaaca gcagtattta tcaagtattc agcacctact tggagatggc ctgacagaat
     2461 tgatcactgt cattaaacaa gctgtgcaga aggttttagg aagtgtttct cttaaacatt
     2521 ctttgtccct tttggacttg gagcaaaaac taaaagaaat cagaaatctc gttgagcagc
     2581 ataagtcttc ttcttggatt aataaagatg gatccaaacc tttattatgc cattatatga
     2641 tgccagatga agaaactcca ttagcagtgc aggcctgtgg actttctcct cgagacatta
     2701 ccactattaa acttctcaat gaaactagag acatgttgga aagcccagat tttagtacag
     2761 ttttgaatac ctgtttaaac cgaggtttta gtagacttct agacaatatg gctgagttct
     2821 ttcgacctac tgaacaggac ctgcaacatg gtaactctat gaatagtctt tccagtgtca
     2881 gcctgccttt agctaagata attccaatag taaacggaca gatccattca gtttgcagtg
     2941 aaacacctag tcattttgtt caggatctgt tgacaatgga gcaagtgaaa gactttgctg
     3001 ctaatgtgta tgaagctttt agtacccctc agcaactgga gaaatgagat ccgggaattc
     3061 gggtctcgag agcggccgca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     3121 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     3181 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     3241 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     3301 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     3361 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     3421 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     3481 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     3541 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     3601 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     3661 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     3721 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     3781 ggacgagctg tacaagtaag cggccgctac gtaaattccg cccccccccc taacgttact
     3841 ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata
     3901 ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt
     3961 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa
     4021 gcagttcctc tggaagtttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag
     4081 cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca
     4141 cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc
     4201 aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat
     4261 tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta
     4321 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgataa
     4381 taatatagcc accgtcgaca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     4441 cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     4501 ccacaccgtc gacccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     4561 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     4621 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     4681 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     4741 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     4801 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     4861 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     4921 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     4981 ccgcaagccc ggtgcctgag tcgacgataa aataaaagat tttatttagt ctccagaaaa
     5041 aggggggaat gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt
     5101 gcaaggcatg gaaaaataca taactgagaa tagagaagtt cagatcaagg tcaggaacag
     5161 atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct
     5221 cagggccaag aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca
     5281 gttcctgccc cggctcaggg ccaagaacag atggtcccca gatgcggtcc agccctcagc
     5341 agtttctaga gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg
     5401 ccttatttga actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc
     5461 gagctcaata aaagagccca caacccctca ctcggggcgc cagtcctccg attgactgag
     5521 tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc
     5581 gctgttcctt gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcac
     5641 atgcagcatg tatcaaaatt aatttggttt tttttcttaa gtatttacat taaatggcca
     5701 tagttgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     5761 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     5821 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     5881 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     5941 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     6001 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     6061 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     6121 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     6181 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     6241 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     6301 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     6361 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     6421 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     6481 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     6541 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     6601 atgagattat caaaaaggat cttcacctag atccttttgc ggccggccgc aaatcaatct
     6661 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     6721 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     6781 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     6841 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     6901 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     6961 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     7021 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     7081 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     7141 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     7201 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     7261 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     7321 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     7381 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     7441 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     7501 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     7561 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     7621 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttc
//
