LOCUS       SP_pAAV_CAG2_EGF        8038 bp    DNA        circular     16-JAN-2024
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 23, 2021 from SnapGene Viewer 5.2.4 to Vector
            NTI(R) format https://www.snapgene.com
COMMENT     VNTDATE|944784000|
COMMENT     VNTDBDATE|944784000|
COMMENT     VNTNAME|SP pAAV CAG2 nAv|
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             1..140
                     /codon_start="1"
                     /note="/ApEinfo_fwdcolor=pink"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1}
                     {} 0}width 5 offset 0"
                     /note="/ApEinfo_revcolor=pink"
                     /note="/vntifkey=4"
                     /locus_tag="Left ITR"
                     /label="Left ITR"
                     /ApEinfo_label="Left ITR"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             1296..2012
                     /note="mammalian codon-optimized"
                     /product="enhanced GFP"
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGK
                     LTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
                     FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQ
                     KNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKR
                     DHMVLLEFVTAAGITLGMDELYK*"
                     /locus_tag="EGFP"
                     /label="EGFP"
                     /ApEinfo_label="EGFP"
                     /ApEinfo_fwdcolor="#05fd14"
                     /ApEinfo_revcolor="#05fd14"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     promoter        279..1248
                     /note="/ApEinfo_fwdcolor=pink"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1}
                     {} 0}width 5 offset 0"
                     /note="/ApEinfo_revcolor=pink"
                     /note="/vntifkey=29"
                     /locus_tag="CAG2 promoter"
                     /label="CAG2 promoter"
                     /ApEinfo_label="CAG2 promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             2013..2075
                     /codon_start="1"
                     /note="/vntifkey=4"
                     /locus_tag="T2A"
                     /label="T2A"
                     /ApEinfo_label="T2A"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             2079..3050
                     /codon_start="1"
                     /note="/ApEinfo_fwdcolor=#e9d024"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1}
                     {} 0}width 5 offset 0"
                     /note="/ApEinfo_revcolor=#e9d024"
                     /note="/vntifkey=4"
                     /locus_tag="HAPLN1"
                     /label="HAPLN1"
                     /ApEinfo_label="HAPLN1"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             2079..2126
                     /codon_start="1"
                     /note="/ApEinfo_fwdcolor=#e9d024"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1}
                     {} 0}width 5 offset 0"
                     /note="/ApEinfo_revcolor=#e9d024"
                     /note="/vntifkey=4"
                     /locus_tag="Endogenous SP"
                     /label="Endogenous SP"
                     /ApEinfo_label="Endogenous SP"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             2136..3026
                     /codon_start="1"
                     /note="/vntifkey=4"
                     /locus_tag="Halo"
                     /label="Halo"
                     /ApEinfo_label="Halo"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             3033..3047
                     /codon_start="1"
                     /note="/vntifkey=4"
                     /locus_tag="Gly linker"
                     /label="Gly linker"
                     /ApEinfo_label="Gly linker"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             4173..4244
                     /codon_start="1"
                     /note="/ApEinfo_fwdcolor=pink"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1}
                     {} 0}width 5 offset 0"
                     /note="/ApEinfo_revcolor=pink"
                     /note="/vntifkey=4"
                     /locus_tag="3x Flag"
                     /label="3x Flag"
                     /ApEinfo_label="3x Flag"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             4377..4967
                     /codon_start="1"
                     /note="/ApEinfo_fwdcolor=pink"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1}
                     {} 0}width 5 offset 0"
                     /note="/ApEinfo_revcolor=pink"
                     /note="/vntifkey=4"
                     /locus_tag="WPRE"
                     /label="WPRE"
                     /ApEinfo_label="WPRE"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    4989..5215
                     /note="/ApEinfo_fwdcolor=#00ffff"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1}
                     {} 0}width 5 offset 0"
                     /note="/ApEinfo_revcolor=green"
                     /note="/vntifkey=21"
                     /locus_tag="PolyA tail"
                     /label="PolyA tail"
                     /ApEinfo_label="PolyA tail"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             5301..5440
                     /codon_start="1"
                     /note="/ApEinfo_fwdcolor=pink"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1}
                     {} 0}width 5 offset 0"
                     /note="/ApEinfo_revcolor=pink"
                     /note="/vntifkey=4"
                     /locus_tag="Right ITR"
                     /label="Right ITR"
                     /ApEinfo_label="Right ITR"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    5516..5971
                     /note="/ApEinfo_fwdcolor=#00ffff"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1}
                     {} 0}width 5 offset 0"
                     /note="/ApEinfo_revcolor=green"
                     /note="/vntifkey=21"
                     /locus_tag="M13 origin"
                     /label="M13 origin"
                     /ApEinfo_label="M13 origin"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             6556..7215
                     /codon_start="1"
                     /note="/ApEinfo_fwdcolor=pink"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1}
                     {} 0}width 5 offset 0"
                     /note="/ApEinfo_revcolor=pink"
                     /note="/vntifkey=4"
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             3051..4163
                     /locus_tag="mHAPLN4 mat pep"
                     /label="mHAPLN4 mat pep"
                     /ApEinfo_label="mHAPLN4 mat pep"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccaat tcagtcgata actataacgg tcctaaggta
      181 gcgatttaaa tacgcgctct cttaaggtag ccccgggacg cgtcaattgg gtaccaagct
      241 tggatccgat atcgcggccg ccctaggctc gagagatctc gacattgatt attgactagt
      301 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
      361 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
      421 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
      481 gtggactatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
      541 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
      601 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
      661 gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca
      721 attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg
      781 gggcgcgcgc caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt
      841 gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
      901 cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gacgctgcct
      961 tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc
     1021 gttactccca caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt
     1081 ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa agccttgagg ggctccggga
     1141 gggccctttg tgcgggggga gcggctcggg gctgtccgcg gggggacggc tgccttcggg
     1201 ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agaatacaag
     1261 ctacttgttc tttttgcagg atccggccgg ccaccATGGT GAGCAAGGGC GAGGAGCTGT
     1321 TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC CACAAGTTCA
     1381 GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG AAGTTCATCT
     1441 GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG ACCTACGGCG
     1501 TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC AAGTCCGCCA
     1561 TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC AACTACAAGA
     1621 CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG CTGAAGGGCA
     1681 TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC TACAACAGCC
     1741 ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT CAAGGTGAAC TTCAAGATCC
     1801 GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA CTACCAGCAG AACACCCCCA
     1861 TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCACCCAG TCCGCCCTGA
     1921 GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG ACCGCCGCCG
     1981 GGATCACTCT CGGCATGGAC GAGCTGTACA AGggcagtgg agagggcaga ggaagtctgc
     2041 taacatgcgg tgacgtcgag gagaatcctg gccccaccat gagaagtctg cttctcctgg
     2101 tgctgatttc agtctgttgg gctgacggcg aattcatggc agaaatcggt actggctttc
     2161 cattcgaccc ccattatgtg gaagtcctgg gcgagcgcat gcactacgtc gatgttggtc
     2221 cgcgcgatgg cacccctgtg ctgttcctgc acggtaaccc gacctcctcc tacgtgtggc
     2281 gcaacatcat cccgcatgtt gcaccgaccc atcgctgcat tgctccagac ctgatcggta
     2341 tgggcaaatc cgacaaacca gacctgggtt atttcttcga cgaccacgtc cgcttcatgg
     2401 atgccttcat cgaagccctg ggtctggaag aggtcgtcct ggtcattcac gactggggct
     2461 ccgctctggg tttccactgg gccaagcgca atccagagcg cgtcaaaggt attgcattta
     2521 tggagttcat ccgccctatc ccgacctggg acgaatggcc agaatttgcc cgcgagacct
     2581 tccaggcctt ccgcaccacc gacgtcggcc gcaagctgat catcgatcag aacgttttta
     2641 tcgagggtac gctgccgatg ggtgtcgtcc gcccgctgac tgaagtcgag atggaccatt
     2701 accgcgagcc gttcctgaat cctgttgacc gcgagccact gtggcgcttc ccaaacgagc
     2761 tgccaatcgc cggtgagcca gcgaacatcg tcgcgctggt cgaagaatac atggactggc
     2821 tgcaccagtc ccctgtcccg aagctgctgt tctggggcac cccaggcgtt ctgatcccac
     2881 cggccgaagc cgctcgcctg gccaaaagcc tgcctaactg caaggctgtg gacatcggcc
     2941 cgggtctgaa tctgctgcaa gaagacaacc cggacctgat cggcagcgag atcgcgcgct
     3001 ggctgtcgac gctcgagatt tccggcgtta acggcggtgg tggtggatcc cagcgtggca
     3061 ggaagaaagt cgtgcacgtg ctggagggcg agtccggttc cgtggtggtg cagacggcgc
     3121 cggggcaggt ggtgagccac cgcggtggta ccattgtctt accctgccgt taccactacg
     3181 aggcggccgc ccacggtcac gacggcgtcc gcctcaagtg gacgaaagtg gtagaccctt
     3241 tggctttcgc cgatgtcttc gtggctctgg gtccccagca tcgagccttc ggtccctacc
     3301 gagggcgggc cgaactgcag aatgacggac ctggggatgc ctccctggtc ctccgaaatg
     3361 tcacccttca ggactacggg cgctacgagt gcgaggtcac caatgagctg gaggatgacg
     3421 tcggcatggt caagctggat ctggaagggg tggtcttccc ctatcacccg cgcggaggtc
     3481 gctacaagat gactttcgtg gaagcgcagc gtgcttgtgc cgaacaggat ggcattttgg
     3541 cgtccgcgga gcagctgcac gcggcctggc gcgatggcct ggactggtgc aacgcgggat
     3601 ggttacgcga tggctcggtg cagtacccag tgagccatgc ccgggagccc tgtggcggga
     3661 ctgggagcac tggagctggt ggcggcacca acgggggcgt gcgcaactat ggatatcgtc
     3721 ataacgctga ggaacgctac gatgccttct gcttcacgtc caacctcccg ggccgcgtgt
     3781 ttttcctgaa accgctgcga cctgtggcct tggccggggc agtgcgcgcg tgcgctgcgc
     3841 ggggtgccac agtggccaag gtcgggcagc tgttcgctgc gtggaagctg cagctgttgg
     3901 accgctgcac tgcaggctgg ctggcggatg gcagcgcgcg ctatcccatc gtgaatccgc
     3961 gaactcgctg tggaggtcct cggcccggcg tgcgcagcct gggtttccct gacgcctccc
     4021 gacgtctctt cggtgtctac tgctaccgcg ctccaggtgc cccagatccg gctccaggcg
     4081 gctggggctg gggctgggcc ggcggaggag gttgggctgg agggtcgagg gatccggctg
     4141 cctggacccc gctgcgtgta tagggcgcgc ccgactacaa ggacgacgac gacaaggact
     4201 acaaggacga cgacgacaag gactacaagg acgacgacga caagtgaccc gggtctagaa
     4261 ctatagtgag tcgtattacg ctagagggcc cttcgaaggt aagcctatcc ctaaccctct
     4321 cctcggtctc gattctacgc gtaccggtca tcatcaccat caccattgag tttcgataat
     4381 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     4441 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     4501 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     4561 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     4621 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     4681 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     4741 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     4801 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     4861 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     4921 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg aaacccgctg
     4981 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     5041 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     5101 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     5161 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     5221 tgaggcggaa agaaccagat cctctcttaa ggtagcatcg agatttaaat tagggataac
     5281 agggtaatgg cgcgggccgc aggaacccct agtgatggag ttggccactc cctctctgcg
     5341 cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg
     5401 ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt
     5461 tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg
     5521 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     5581 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     5641 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     5701 ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg
     5761 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     5821 ttgttccaaa ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg
     5881 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     5941 aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct
     6001 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     6061 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     6121 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     6181 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     6241 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     6301 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     6361 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     6421 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     6481 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     6541 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     6601 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     6661 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     6721 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     6781 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     6841 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     6901 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     6961 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     7021 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     7081 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     7141 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     7201 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     7261 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     7321 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     7381 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     7441 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     7501 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     7561 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     7621 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     7681 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     7741 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     7801 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     7861 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     7921 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     7981 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt
//
