LOCUS       Exported               13256 bp ds-DNA    circular SYN 03-AUG-2024
DEFINITION  pXH150-PB-3xFL-mRnaseh1(hygro).dna (8510 bp).
ACCESSION   .
VERSION     .
KEYWORDS    G151-pB-3xFL-dCas13-mTET2HxDCD-Hyg
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13256)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sunday, Oct 6, 2024 from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13256
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          4903..7503
     enhancer        30..409
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        412..687
                     /note="chicken beta-actin promoter"
     intron          688..1696
                     /note="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     protein_bind    1774..1798
                     /gene="mutant version of attB"
                     /bound_moiety="BP Clonase(TM)"
                     /note="attB1"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1807..1872
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /note="3xFLAG"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1903..1923
                     /codon_start=1
                     /locus_tag="
                     "
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /note="SV40 NLS"
                     /note="
                     "
                     /protein_id="
                     "
                     /translation="PKKKRKV"
     misc_feature    1924..4902
                     /note="dCas13b"
     misc_feature    4903..7503
                     /label="{3xFlag mTET2HxDCD(ns)}"
                     /label="nonstandard type: ORF"
                     /note="{3xFlag mTET2HxDCD(ns)}"
                     /note="/application_notes="
                     /note="/official_designation={3xFlag mTET2HxDCD(ns)}"
                     /note="/uuid=ccbf714e1fc0464795851798393399ea"
     misc_feature    5650..5658
                     /note="YxA"
     protein_bind    complement(7516..7540)
                     /gene="mutant version of attB"
                     /bound_moiety="BP Clonase(TM)"
                     /note="attB2"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(7561..7577)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7585..7601
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7609..7639)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    7654..7675
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    7741..7965
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(8042..8075)
                     /bound_moiety="Cre recombinase"
                     /note="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     promoter        complement(8299..8317)
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8327..8343)
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      8485..8940
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        8966..9070
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             9071..9931
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      10102..10690
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    10978..10999
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        11014..11044
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    11052..11068
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11076..11092
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        11113..11131
                     /note="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(11671..12696)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /note="HygR"
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(12715..13214)
                     /note="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     protein_bind    complement(13237..14)
                     /bound_moiety="Cre recombinase"
                     /note="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
ORIGIN
        1 ctatacgaag ttatgcggcc ctcgaggtcg acattgatta ttgactagtt attaatagta
       61 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
      121 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
      181 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggactattt
      241 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
      301 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
      361 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg gtcgaggtga
      421 gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt
      481 tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg gcgcgcgcca
      541 ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc
      601 aatcagagcg gcgcgctccg aaagtttcct tttatggcga ggcggcggcg gcggcggccc
      661 tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcgt tgccttcgcc ccgtgccccg
      721 ctccgcgccg cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg
      781 agcgggcggg acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctc
      841 gtttcttttc tgtggctgcg tgaaagcctt aaagggctcc gggagggccc tttgtgcggg
      901 ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggccc
      961 gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcgtg
     1021 tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg cgggggggct gcgaggggaa
     1081 caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcggcggt
     1141 cgggctgtaa cccccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg
     1201 gtgcggggct ccgtgcgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc
     1261 aggtgggggt gccgggcggg gcggggccgc ctcgggccgg ggagggctcg ggggaggggc
     1321 gcggcggccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt
     1381 atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctggc ggagccgaaa
     1441 tctgggaggc gccgccgcac cccctctagc gggcgcgggc gaagcggtgc ggcgccggca
     1501 ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccatc
     1561 tccagcctcg gggctgccgc agggggacgg ctgccttcgg gggggacggg gcagggcggg
     1621 gttcggcttc tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt
     1681 cttctttttc ctacagctcc tgggcaacgt gctggttgtt gtgctgtctc atcattttgg
     1741 caaagaattc ctcgacggta tcgataagct atcacaagtt tgtacaaaaa agcaggcttc
     1801 accatggact acaaagacca tgacggtgat tataaagatc atgatatcga ttacaaggat
     1861 gacgatgaca agggatccgg cggcggcggc ggcgccgcta gcccaaagaa gaagcggaaa
     1921 gtcaacatcc ccgctctggt ggaaaaccag aagaagtact ttggcaccta cagcgtgatg
     1981 gccatgctga acgctcagac cgtgctggac cacatccaga aggtggccga tattgagggc
     2041 gagcagaacg agaacaacga gaatctgtgg tttcaccccg tgatgagcca cctgtacaac
     2101 gccaagaacg gctacgacaa gcagcccgag aaaaccatgt tcatcatcga gcggctgcag
     2161 agctacttcc cattcctgaa gatcatggcc gagaaccaga gagagtacag caacggcaag
     2221 tacaagcaga accgcgtgga agtgaacagc aacgacatct tcgaggtgct gaagcgcgcc
     2281 ttcggcgtgc tgaagatgta cagggacctg accaacgcat acaagaccta cgaggaaaag
     2341 ctgaacgacg gctgcgagtt cctgaccagc acagagcaac ctctgagcgg catgatcaac
     2401 aactactaca cagtggccct gcggaacatg aacgagagat acggctacaa gacagaggac
     2461 ctggccttca tccaggacaa gcggttcaag ttcgtgaagg acgcctacgg caagaaaaag
     2521 tcccaagtga ataccggatt cttcctgagc ctgcaggact acaacggcga cacacagaag
     2581 aagctgcacc tgagcggagt gggaatcgcc ctgctgatct gcctgttcct ggacaagcag
     2641 tacatcaaca tctttctgag caggctgccc atcttctcca gctacaatgc ccagagcgag
     2701 gaacggcgga tcatcatcag atccttcggc atcaacagca tcaagctgcc caaggaccgg
     2761 atccacagcg agaagtccaa caagagcgtg gccatggata tgctcaacga agtgaagcgg
     2821 tgccccgacg agctgttcac aacactgtct gccgagaagc agtcccggtt cagaatcatc
     2881 agcgacgacc acaatgaagt gctgatgaag cggagcagcg acagattcgt gcctctgctg
     2941 ctgcagtata tcgattacgg caagctgttc gaccacatca ggttccacgt gaacatgggc
     3001 aagctgagat acctgctgaa ggccgacaag acctgcatcg acggccagac cagagtcaga
     3061 gtgatcgagc agcccctgaa cggcttcggc agactggaag aggccgagac aatgcggaag
     3121 caagagaacg gcaccttcgg caacagcggc atccggatca gagacttcga gaacatgaag
     3181 cgggacgacg ccaatcctgc caactatccc tacatcgtgg acacctacac acactacatc
     3241 ctggaaaaca acaaggtcga gatgtttatc aacgacaaag aggacagcgc cccactgctg
     3301 cccgtgatcg aggatgatag atacgtggtc aagacaatcc ccagctgccg gatgagcacc
     3361 ctggaaattc cagccatggc cttccacatg tttctgttcg gcagcaagaa aaccgagaag
     3421 ctgatcgtgg acgtgcacaa ccggtacaag agactgttcc aggccatgca gaaagaagaa
     3481 gtgaccgccg agaatatcgc cagcttcgga atcgccgaga gcgacctgcc tcagaagatc
     3541 ctggatctga tcagcggcaa tgcccacggc aaggatgtgg acgccttcat cagactgacc
     3601 gtggacgaca tgctgaccga caccgagcgg agaatcaaga gattcaagga cgaccggaag
     3661 tccattcgga gcgccgacaa caagatggga aagagaggct tcaagcagat ctccacaggc
     3721 aagctggccg acttcctggc caaggacatc gtgctgtttc agcccagcgt gaacgatggc
     3781 gagaacaaga tcaccggcct gaactaccgg atcatgcaga gcgccattgc cgtgtacgat
     3841 agcggcgacg attacgaggc caagcagcag ttcaagctga tgttcgagaa ggcccggctg
     3901 atcggcaagg gcacaacaga gcctcatcca tttctgtaca aggtgttcgc ccgcagcatc
     3961 cccgccaatg ccgtcgagtt ctacgagcgc tacctgatcg agcggaagtt ctacctgacc
     4021 ggcctgtcca acgagatcaa gaaaggcaac agagtggatg tgcccttcat ccggcgggac
     4081 cagaacaagt ggaaaacacc cgccatgaag accctgggca gaatctacag cgaggatctg
     4141 cccgtggaac tgcccagaca gatgttcgac aatgagatca agtcccacct gaagtccctg
     4201 ccacagatgg aaggcatcga cttcaacaat gccaacgtga cctatctgat cgccgagtac
     4261 atgaagagag tgctggacga cgacttccag accttctacc agtggaaccg caactaccgg
     4321 tacatggaca tgcttaaggg cgagtacgac agaaagggct ccctgcagca ctgcttcacc
     4381 agcgtggaag agagagaagg cctctggaaa gagcgggcct ccagaacaga gcggtacaga
     4441 aagcaggcca gcaacaagat ccgcagcaac cggcagatga gaaacgccag cagcgaagag
     4501 atcgagacaa tcctggataa gcggctgagc aacagccgga acgagtacca gaaaagcgag
     4561 aaagtgatcc ggcgctacag agtgcaggat gccctgctgt ttctgctggc caaaaagacc
     4621 ctgaccgaac tggccgattt cgacggcgag aggttcaaac tgaaagaaat catgcccgac
     4681 gccgagaagg gaatcctgag cgagatcatg cccatgagct tcaccttcga gaaaggcggc
     4741 aagaagtaca ccatcaccag cgagggcatg aagctgaaga actacggcga cttctttgtg
     4801 ctggctagcg acaagaggat cggcaacctg ctggaactcg tgggcagcga catcgtgtcc
     4861 aaagaggatg gatccaaaag aaccgccgac ggcagcgaat tcggcaaatg tgaaggatgc
     4921 aatccagaca aagatgaagc tccttattat acccatctag gagctggtcc tgatgtggca
     4981 gctattagaa cactcatgga agaaaggtat ggagagaagg gtaaagctat taggattgaa
     5041 aaagtcatat atactggtaa agaaggcaag agctctcagg gatgtcctat tgctaaatgg
     5101 gtatatcgga gatcgagtga ggaggagaaa ctactgtgtt tggtacgagt gcgacctaat
     5161 cacacatgtg agacggcggt gatggtaatt gccatcatgt tgtgggacgg aatcccaaag
     5221 ctactcgcat cagaactcta ctcagaactt acagatatct tgggcaagtg tggcatatgc
     5281 accaaccgtc gctgttctca gaatgaaact agaaactgtt gttgtcaggg tgagaatcca
     5341 gagacctgtg gtgcctcctt ttcttttggt tgttcttgga gcatgtacta taatggatgt
     5401 aagtttgcca gaagcaagaa accaaggaaa tttaggctac atggagctga gccaaaagag
     5461 gaagagagac taggttctca tttgcaaaac ctggctactg tcattgctcc aatatacaag
     5521 aagcttgcac ccgatgcata caataatcag gttgaatttg aacaccaagc cccagactgc
     5581 tgtttgggtc tgaaggaagg ccggccattc tcaggagtca ctgcatgttt ggacttctct
     5641 gctcattcct acagagcaca gcagaacatg ccaaatggca gtacagtggt ggtcaccctc
     5701 aatagagaag acaatcgaga agtcggagct aagcctgagg atgagcagtt ccacgtgctg
     5761 cctatgtaca tcatcgcccc tgaggatgag tttgggagta cggaaggcca ggagaagaag
     5821 atacggatgg ggtccattga ggttctgcag tcatttcgga ggagaagggt cataaggata
     5881 ggagagctgc ccaagagttg caagaagaaa gcggagccca agaaagccaa gaccaagaaa
     5941 gcagctcgaa agcgttcctc tctggagaac tgctccagta ggactgagaa gggaaagtct
     6001 tcctcacata caaagctgat ggaaaatgca agccatatga aacaaatgac agcacaaccg
     6061 cagctttcgg gcccggtcat ccggcagcca ccaacactcc agaggcacct tcagcaaggg
     6121 cagaggccac agcagccgca gccacctcag ccgcagccgc agacgacacc tcagccacag
     6181 ccacagccac agcatatcat gcccggtaac tctcagtctg ttggttctca ttgttctgga
     6241 tccaccagtg tctacacgag acagcctact cctcacagtc cttatcccag ctcagcacac
     6301 acctcagata tttatggaga taccaaccat gtgaactttt accccacttc atctcatgcc
     6361 tcgggttcat atttgaatcc ttctaattac atgaacccct accttgggct tttgaatcag
     6421 aataaccaat atgcaccttt tccatacaat gggagtgtgc cagtggacaa tggttcccct
     6481 ttcttaggtt cttattcccc ccaggctcag tccagggatc tacatagata tccaaaccag
     6541 gaccatctca ccaatcagaa cttaccaccc atccacaccc ttcaccaaca gacgtttggg
     6601 gacagtccct ctaagtactt aagttatgga aaccaaaata tgcagagaga tgccttcact
     6661 actaactcca ccctaaaacc aaatgtacac cacctagcaa cgttttctcc ttaccccacc
     6721 cccaagatgg atagtcattt catgggagct gcctccagat caccatacag ccacccacac
     6781 actgactaca aaaccagtga gcatcatcta ccctctcaca cgatctacag ctacacggca
     6841 gcagcttcgg ggagcagttc cagccacgcc ttccacaaca aggagaatga caacatagcc
     6901 aatgggctct caagagtgct tccagggttt aatcatgata gaactgcttc tgcccaagaa
     6961 ctattataca gtctgactgg cagcagtcag gagaagcagc ctgaggtgtc aggccaggat
     7021 gcagctgctg tgcaggaaat tgagtattgg tcagatagtg agcacaactt tcaggatcct
     7081 tgcattggag gggtggctat agctccaact catgggtcaa ttcttattga gtgtgcaaag
     7141 tgtgaggttc atgccacaac caaagtaaac gatcccgacc ggaatcaccc caccaggatc
     7201 tcacttgtac tgtataggca taagaatttg tttctaccaa aacattgttt ggctctctgg
     7261 gaagccaaaa tggctgaaaa ggcccggaaa gaggaagagt gcggaaagaa tggatcagac
     7321 cacgtgtctc agaaaaatca tggcaaacag gaaaagcgtg agcccacagg gccacaggaa
     7381 cccagttacc tgcgtttcat ccagtctctt gctgagaaca cagggtctgt gactacggat
     7441 tctaccgtga ctacatcacc atatgctttc actcaggtca cagggcctta caacacattt
     7501 gtatgattaa ttaagaccca gctttcttgt acaaagtggt gatagcttgg cgtaatcatg
     7561 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     7621 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     7681 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga tcagcctcga
     7741 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     7801 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     7861 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     7921 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     7981 gaaccagctg gggctcgaga tccactagtt ctagcctcga ggctagagcg gccgcgtcga
     8041 gataacttcg tataatgtat gctatacgaa gttatgtttt gttactttat agaagaaatt
     8101 ttgagttttt gttttttttt aataaataaa taaacataaa taaattgttt gttgaattta
     8161 ttattagtat gtaagtgtaa atataataaa acttaatatc tattcaaatt aataaataaa
     8221 cgtcaatttt acgcatgatt atctttaacg tacgtcacaa tatgattatc tttctagggt
     8281 taaagagctc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     8341 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     8401 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     8461 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     8521 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     8581 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     8641 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     8701 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     8761 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     8821 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     8881 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     8941 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     9001 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     9061 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     9121 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     9181 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     9241 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     9301 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     9361 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     9421 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     9481 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     9541 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     9601 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     9661 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     9721 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     9781 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     9841 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     9901 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     9961 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
    10021 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
    10081 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
    10141 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
    10201 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
    10261 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
    10321 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
    10381 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
    10441 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
    10501 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
    10561 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
    10621 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
    10681 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
    10741 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
    10801 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
    10861 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
    10921 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
    10981 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
    11041 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
    11101 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctgggtacct taaccctaga
    11161 aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat attgctctct ctttctaaat
    11221 agcgcgaatc cgtcgctgtg catttaggac atctcagtcg ccgcttggag ctcccgtgag
    11281 gcgtgcttgt caatgcggta agtgtcactg attttgaact ataacgaccg cgtgagtcaa
    11341 aatgacgcat gattatcttt tacgtgactt ttaagattta actcatacga taattatatt
    11401 gttatttcat gttctactta cgtgataact tattatatat atattttctt gttatagata
    11461 tccaactatg aaacattatc ataaagcaat agtaaagagc cttcagtaaa gagcaggcat
    11521 ttatctaatc ccaccccacc cccacccccg tagctccaat ccttccattc aaaatgtagg
    11581 tactctgttc tcacccttct taacaaagta tgacaggaaa aaacttccat gtggacatct
    11641 ttattgttta atagatcatc aatttctcga ctattccttt gccctcggac gagtgctggg
    11701 gcgtcggttt ccactatcgg cgagtacttc tacacagcca tcggtccaga cggccgcgct
    11761 tctgcgggcg atttgtgtac gcccgacagt cccggctccg gatcggacga ttgcgtcgca
    11821 tcgaccctgc gcccaagctg catcatcgaa attgccgtca accaagctct gatagagttg
    11881 gtcaagacca atgcggagca tatacgcccg gagccgcggc gatcctgcaa gctccggatg
    11941 cctccgctcg aagtagcgcg tctgctgctc catacaagcc aaccacggcc tccagaagaa
    12001 gatgttggcg acctcgtatt gggaatcccc gaacatcgcc tcgctccagt caatgaccgc
    12061 tgttatgcgg ccattgtccg tcaggacatt gttggagccg aaatccgcgt gcacgaggtg
    12121 ccggacttcg gggcagtcct cggcccaaag catcagctca tcgagagcct gcgcgacgga
    12181 cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac acatggggat cagcaatcgc
    12241 gcatatgaaa tcacgccatg tagtgtattg accgattcct tgcggtccga atgggccgaa
    12301 cccgctcgtc tggctaagat cggccgcagc gatcgcatcc atggcctccg cgaccggctg
    12361 cagaacagcg ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct gtgcacggcg
    12421 ggagatgcaa taggtcaggc tctcgctgaa ttccccaatg tcaagcactt ccggaatcgg
    12481 gagcgcggcc gatgcaaagt gccgataaac ataacgatct ttgtagaaac catcggcgca
    12541 gctatttacc cgcaggacat atccacgccc tcctacatcg aagctgaaag cacgagattc
    12601 ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg aacttttcga tcagaaactt
    12661 ctcgacagac gtcgcggtga gttcaggctt tttcatggtg gcggctggat cggtcgaaag
    12721 gcccggagat gaggaagagg agaacagcgc ggcagacgtg cgcttttgaa gcgtgcgaga
    12781 atgccgggcc tccggaggac cttcgggcgc ccgccccgcc cctgagcccg cccctgagcc
    12841 cgcccccgga cccacccctt cccagcctct gagcccagaa agcgaaggag caaagctgct
    12901 attggccgct gccccaaagg cctacccgct tccattgctc agcggtgctg tccatctgca
    12961 cgagactagt gagacgtgct acttccattt gtcacgtcct gcacgacgcg agctgcgggg
    13021 cgggggggaa cttcctgact aggggaggag tagaaggtgg cgcgaagggg ccaccaaaga
    13081 acggagccgg ttggcgccta ccggtggatg tggaatgtgt gcgaggccag aggccacttg
    13141 tgtagcgcca agtgccagcg gggctgctaa agcgcatgct ccagactgcc ttgggaaaag
    13201 cgcctcccct acccggtaga attcatcgat gatatcataa cttcgtataa tgtatg
//
