LOCUS       pHPHS927_1_cHS4_        6642 bp DNA     circular SYN 24-JAN-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 6, 2024 from SnapGene 7.0.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6642
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          9..1219
                     /mol_type="other DNA"
                     /organism="pC13N-iCAG.copGFP"
     source          1621..2917
                     /mol_type="other DNA"
                     /organism="pLJM1-EGFP"
     source          1624..1689
                     /mol_type="other DNA"
                     /organism="AAVS1_Puro_PGK1_3xFLAG_Twin_Strep"
     source          1940..1955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     source          2924..3201
                     /mol_type="other DNA"
                     /label=source
                     /organism="AAVS1_Puro_PGK1_3xFLAG_Twin_Strep"
     source          2954..2959
                     /mol_type="other DNA"
                     /organism="AAVS1_Puro_PGK1_3xFLAG_Twin_Strep"
     source          2960..2977
                     /mol_type="other DNA"
                     /organism="pGL3-Control"
     source          4021..4620
                     /label=source
     source          4631..4643
                     /mol_type="other DNA"
                     /organism="AAVS1_Puro_PGK1_3xFLAG_Twin_Strep"
     primer_bind     complement(1..11)
                     /label=oAS1882
                     /note="Fluc_NotI_F"
     primer_bind     4..47
                     /label=oAS1507
                     /note="attBL_cHS4_F"
     misc_feature    9..1219
                     /label=cHS4_insulator
                     /label=insulator
                     /note="/created_by=User"
     primer_bind     complement(179..196)
                     /label=oAS1601
                     /note="cHS4_start_R"
     primer_bind     359..368
                     /label=oAS1564
                     /note="bglobin39_R"
     primer_bind     complement(1185..1220)
                     /label=oAS1510
                     /note="CMVenhancer_EcoRI_cHS4_R"
     primer_bind     complement(1202..1220)
                     /label=oAS1473
                     /note="pPGK1_cHS4_R"
     primer_bind     1242..1258
                     /label=oAS1468
                     /note="attB_pTet_F"
     promoter        1259..1608
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     promoter        1263..1608
                     /label=PTRE3G
     protein_bind    1267..1285
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1303..1321
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1339..1357
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1375..1393
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1411..1429
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1447..1465
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1483..1501
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1502..1518
                     /label=oAS1463
                     /note="pTet_End_Seq_F"
     primer_bind     1513..1522
                     /label=CMV_F
     primer_bind     complement(1544..1572)
                     /label=oPN211
     5'UTR           1565..1620
                     /note="based on homology to CMV promoter near SacI site"
     primer_bind     complement(1585..1624)
                     /label=oAS1469
                     /note="pTREend_hind3_kozak_r"
     CDS             join(1621..1784,1915..2137,2792..2923)
                     /codon_start=1
                     /translation="MDYKDHDGDYKDHDIDYKDDDDKVDVHLTPEEKSAVTALWGKVNV
                     DEVGGEALGRLLVVYPWTQRFFESFGDLSTPDAVMGNPKVKAHGKKVLGAFSDGLAHLD
                     NLKGTFATLSELHCDKLHVDPENFRLLGNMIVIVLGHHLGKDFTPAAQAAFQKVVAGVA
                     TALAHKYHGT"
     CDS             1624..1689
                     /label=3xflag
                     /note="/modified_by=User"
                     /note="/source=Addgene # 68375"
     exon            1696..1784
                     /label=Hs beta-globin exon 1
                     /note="/created_by=User"
     intron          1785..1914
                     /label=Hs beta-globin intron 1
                     /note="/created_by=User"
                     /note="/modified_by=User"
     primer_bind     1815..1832
                     /label=oAS498
     primer_bind     complement(1914..1939)
                     /label=oAS1564
                     /note="bglobin39_R"
     exon            1915..2137
                     /label=Hs beta-globin exon 2
                     /note="/created_by=User"
                     /note="/modified_by=User"
     primer_bind     1917..1976
                     /label=oAS1565
                     /note="bglobin_ptc39_f"
     primer_bind     1917..1976
                     /label=oAS1566
                     /note="bglobin_normal39_f"
     misc_feature    1940
                     /label=C>T PTC39
     primer_bind     1943..1976
                     /label=oAS1614
                     /note="EYFP_bglobine2_f"
     primer_bind     1956..1983
                     /label=oAS508
     primer_bind     2026..2045
                     /label=RKB960
     primer_bind     2114..2137
                     /label=oAS489
     primer_bind     complement(2114..2137)
                     /label=oAS488
     misc_feature    2119..2124
                     /label=Hs-Mm junction
                     /note="/created_by=User"
     intron          2138..2791
                     /label=Mm beta-globin intron 2
                     /note="/created_by=User"
                     /note="/modified_by=User"
     primer_bind     2685..2703
                     /label=oAS493
     misc_difference 2694
                     /label='C' in primer
                     /note="/created_by=User"
     primer_bind     2718..2744
                     /label=oPN787
     primer_bind     2752..2779
                     /label=oPN788
     primer_bind     complement(2768..2800)
                     /label=custom_i7_r1
     primer_bind     2768..2792
                     /label=oPN789
     primer_bind     2791..2815
                     /label=oAS489
     primer_bind     2791..2804
                     /label=RKB947
     exon            2792..2917
                     /label=Mm beta-globin exon 3
                     /note="/created_by=User"
     primer_bind     complement(2803..2823)
                     /label=RKB961
     primer_bind     2826..2839
                     /label=oAS1292
                     /note="remove_bsmb1_puro_f"
     primer_bind     complement(2895..2979)
                     /label=oAS1615
                     /note="FLucend_bglobine3_R"
     primer_bind     complement(2895..2923)
                     /label=oAS1567
                     /note="EYFP_KpnI_Mmbglobin_e3_R"
     primer_bind     2900..2953
                     /label=oAS1868
                     /note="Mmbglobin_HA_F"
     CDS             2924..2950
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(2930..2979)
                     /label=oAS1870
                     /note="HA_ACAGTG_Flucend_R"
     misc_feature    2954..2959
                     /label=barcode
     primer_bind     2960..3003
                     /label=oAS1554
                     /note="Flucend_NotI_bghpA_start_F"
     CDS             2960..2977
                     /codon_start=1
                     /label=end_of_fluc
                     /label=ORF frame 1
                     /translation="GKIAV"
     gene            2960..2977
                     /gene="firefly_Luciferase"
                     /label=firefly_Luciferase
     terminator      2986..3201
                     /label=bGH_PA_terminator
     primer_bind     2986..3006
                     /label=oPN202
     primer_bind     complement(3141..3154)
                     /label=oPB hsprimers Rev
     primer_bind     complement(3179..3201)
                     /label=oAS1491
     primer_bind     3182..3219
                     /label=oAS1581
                     /note="attBL_bghpA_R"
     primer_bind     complement(3182..3201)
                     /label=oAS1556
                     /note="AmpRt_XbaI_bghpAend_R"
     primer_bind     3202..3243
                     /label=oAS1482
     misc_feature    3202..3222
                     /label=attB-Left
     primer_bind     complement(3202..3222)
                     /label=oAS1507
                     /note="attBL_cHS4_F"
     primer_bind     3209..3268
                     /label=oAS1675
                     /note="attB_NheI_mCherry_F"
     primer_bind     complement(3215..3259)
                     /label=oAS1672
                     /note="3xflag_attB_R"
     misc_feature    3223..3243
                     /label=attB-Right
     primer_bind     complement(3224..3240)
                     /label=oAS1468
                     /note="attB_pTet_F"
     primer_bind     3246..3263
                     /label=oPB346 CMV_hsmKate_fwd
     CDS             3256..3963
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     regulatory      3256..3259
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     3259..3282
                     /label=oAS1481
     primer_bind     complement(3262..3276)
                     /label=oPB hsprimers Rev
     misc_RNA        3340..3359
                     /label=sgrna_1_wheeler_2020
                     /note="from Wheeler 2020"
     misc_RNA        complement(3345..3364)
                     /label=sgrna_2_wheeler_2020
                     /note="from Wheeler 2020"
     primer_bind     complement(3403..3419)
                     /label=oAS1403
     misc_RNA        3712..3731
                     /label=sgrna_3_designed
     misc_RNA        3904..3923
                     /label=sgrna_1_designed
     primer_bind     3943..3982
                     /label=oAS1311
                     /note="mCherry_T2A_F"
     primer_bind     complement(3943..3963)
                     /label=oAS1310
                     /note="mCherry_end_R"
     CDS             3964..4017
                     /label=T2A
     gene            4021..4620
                     /label=puro (variant)
     CDS             4021..4620
                     /label=puro
     primer_bind     4086..4097
                     /label=oAS1882
                     /note="Fluc_NotI_F"
     primer_bind     complement(4393..4431)
                     /label=oAS1293
                     /note="remove_bsmb1_puro_r"
     primer_bind     4393..4431
                     /label=oAS1292
                     /note="remove_bsmb1_puro_f"
     misc_feature    4506
                     /label=Remove_bsa1_CtoA
     primer_bind     complement(4599..4682)
                     /label=oAS1884
                     /note="puroR_ACAGTG_Fluc_R"
     primer_bind     complement(4604..4651)
                     /label=oAS1361
                     /note="not1_linker_puro_end_R"
     misc_feature    4621..4626
                     /label=barcode
     primer_bind     4627..4651
                     /label=oAS1882
                     /note="Fluc_NotI_F"
     CDS             4631..4643
                     /label=Fluc
                     /note="/created_by=User"
     primer_bind     complement(4634..4673)
                     /label=oAS1582
                     /note="Fluc_BamHI_SV40pAstart_F"
     terminator      4653..4782
                     /label=SV40-PA
     primer_bind     4653..4669
                     /label=oAS1307
     primer_bind     complement(4762..4812)
                     /label=oAS1327
                     /note="AmpRt_SV40pA_R"
     primer_bind     complement(4783..4812)
                     /label=oAS1556
                     /note="AmpRt_XbaI_bghpAend_R"
     terminator      complement(4793..4889)
                     /label=AmpR Terminator
     primer_bind     4793..4812
                     /label=oAS1316
                     /note="_AmpR_R"
     CDS             complement(4890..5747)
                     /label=AmpR
     promoter        complement(5748..5878)
                     /label=AmpR Promoter
     rep_origin      complement(5879..6642)
                     /direction=LEFT
                     /label=ColE1
     primer_bind     6488..6504
                     /label=oAS1602
                     /note="ColE1_end_F"
     primer_bind     complement(6625..6642)
                     /label=oAS1315
                     /note="ColE1_R"
     primer_bind     join(6626..6642,1..43)
                     /label=oAS1579
                     /note="ColE1_NotI_cHS4_F"
ORIGIN
        1 gcggccgcga gctcacgggg acagcccccc cccaaagccc ccagggatgt aattacgtcc
       61 ctcccccgct agggggcagc agcgagccgc ccggggctcc gctccggtcc ggcgctcccc
      121 ccgcatcccc gagccggcag cgtgcgggga cagcccgggc acggggaagg tggcacggga
      181 tcgctttcct ctgaacgctt ctcgctgctc tttgagcctg cagacacctg gggggatacg
      241 gggaaaaagc tttaggctga aagagagatt tagaatgaca gaatcataga acggcctggg
      301 ttgcaaagga gcacagtgct catccagatc caaccccctg ctatgtgcag ggtcatcaac
      361 cagcagccca ggctgcccag agccacatcc agcctggcct tgaatgcctg cagggatggg
      421 gcatccacag cctccttggg caacctgttc agtgcgtcac caccctctgg gggaaaaact
      481 gcctcctcat atccaaccca aacctcccct gtctcagtgt aaagccattc ccccttgtcc
      541 tatcaagggg gagtttgctg tgacattgtt ggtctggggt gacacatgtt tgccaattca
      601 gtgcatcacg gagaggcaga tcttggggat aaggaagtgc aggacagcat ggacgtggga
      661 catgcaggtg ttgagggctc tgggacactc tccaagtcac agcgttcaga acagccttaa
      721 ggataagaag ataggataga aggacaaaga gcaagttaaa acccagcatg gagaggagca
      781 caaaaaggcc acagacactg ctggtccctg tgtctgagcc tgcatgtttg atggtgtctg
      841 gatgcaagca gaaggggtgg aagagcttgc ctggagagat acagctgggt cagtaggact
      901 gggacaggca gctggagaat tgccatgtag atgttcatac aatcgtcaaa tcatgaaggc
      961 tggaaaagcc ctccaagatc cccaagacca accccaaccc acccaccgtg cccactggcc
     1021 atgtccctca gtgccacatc cccacagttc ttcatcacct ccagggacgg tgaccccccc
     1081 acctccgtgg gcagctgtgc cactgcagca ccgctctttg gagaaggtaa atcttgctaa
     1141 atccagcccg accctcccct ggcacaacgt aaggccatta tctctcatcc aactccagga
     1201 cggagtcagt gagaatattg gcgcgccgat atccgatgag gccctttcgt cttcactcga
     1261 gtttactccc tatcagtgat agagaacgta tgaagagttt actccctatc agtgatagag
     1321 aacgtatgca gactttactc cctatcagtg atagagaacg tataaggagt ttactcccta
     1381 tcagtgatag agaacgtatg accagtttac tccctatcag tgatagagaa cgtatctaca
     1441 gtttactccc tatcagtgat agagaacgta tatccagttt actccctatc agtgatagag
     1501 aacgtatgtc gaggtaggcg tgtacggtgg gcgcctataa aagcagagct cgtttagtga
     1561 accgtcagat cgcctggagc aattccacaa cacttttgtc ttatacttaa gcttgccacc
     1621 atggactaca aggaccacga cggcgattat aaggatcacg acatcgacta caaagacgac
     1681 gatgacaagg tcgacgtgca tctgactcct gaggagaagt ctgccgttac tgccctgtgg
     1741 ggcaaggtga acgtggatga agttggtggt gaggccctgg gcaggttggt atcaaggtta
     1801 caagacaggt ttaaggagac caatagaaac tgggcatgtg gagacagaga agactcttgg
     1861 gtttctgata ggcactgact ctctctgcct attggtctat tttcccaccc ttaggctgct
     1921 ggtggtctac ccttggaccc agaggttctt tgagtccttt ggggatctgt ccactcctga
     1981 tgctgttatg ggcaacccta aggtgaaggc tcatggcaag aaagtgctcg gtgcctttag
     2041 tgatggcctg gctcacctgg acaacctcaa gggcaccttt gccacactga gtgagctgca
     2101 ctgtgacaag ctgcacgtgg atcctgagaa cttcagggtg agtctgatgg gcacctcctg
     2161 ggtttccttc ccctggctat tctgctcaac cttcctatca gaaaaaaagg ggaagcgatt
     2221 ctagggagca gtctccatga ctgtgtgtgg agtgttgaca agagttcgga tattttattc
     2281 tctactcaga attgctgctc cccctcactc tgttctgtgt tgtcatttcc tctttctttg
     2341 gtaagctttt aatttccagt tgcattttac taaattaatt aagctggtta tttacttccc
     2401 atcctgatat cagcttcccc tcctcctttc ctcccagtcc ttctctctct cctctctctt
     2461 tctctaatcc tttcctttcc ctcagttcat ttcttcttct ttgatctacg tttgtttgtc
     2521 tttttaaata ttgccttgta acttgctcag aggacaagga agatatgtcc ctgtttcttc
     2581 tcatagctct caagaatagt agcataattg gcttttatgc cagggtgaca ggggaagaat
     2641 atattttaca tataaattct gtttgacata ggattcttat aataatttgt cagtagttta
     2701 aggttgcaaa caaatgtctt tgtaaataag cctgcagtat ctggtatttt tgctctacag
     2761 ttatgttgat ggttcttcca tattcccaca gctcctgggc aatatgatcg tgattgtgct
     2821 gggccaccac ctgggcaagg atttcacccc cgctgcacag gctgccttcc agaaggtggt
     2881 ggctggagtg gccactgccc tggctcacaa gtaccacggt acctacccat acgatgttcc
     2941 ggattacgct tagacagtgg gaaagatcgc cgtgtaagcg gccgcctagt tgccagccat
     3001 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     3061 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     3121 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     3181 gggatgcggt gggctctatg gccggcttgt cgacgacggc ggactccgtc gtcaggatca
     3241 tccgcgctag ccaccatggt gagcaagggc gaggaggata acatggccat catcaaggag
     3301 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag
     3361 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
     3421 ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag
     3481 gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc
     3541 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac
     3601 tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc
     3661 tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg
     3721 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc
     3781 ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc
     3841 ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc
     3901 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac
     3961 aaggagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctagg
     4021 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     4081 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     4141 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     4201 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     4261 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     4321 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     4381 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     4441 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     4501 gagacatccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     4561 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
     4621 acagtgccat cagagagatc ctcgcggccg cggatccaga catgataaga tacattgatg
     4681 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     4741 atgctattgc tttatttgta accattataa gctgcaataa actctagaga gtgattatca
     4801 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     4861 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     4921 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     4981 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcggga cccacgctca
     5041 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     5101 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     5161 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     5221 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     5281 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     5341 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     5401 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     5461 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     5521 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     5581 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     5641 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     5701 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     5761 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     5821 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgtc
     5881 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     5941 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     6001 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     6061 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     6121 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     6181 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     6241 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     6301 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     6361 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     6421 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     6481 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     6541 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     6601 atgttctttc ctgcgttatc ccctgattct gtggataacc gt
//
