LOCUS       H325_mTurq2del_E        9846 bp DNA     circular SYN 07-OCT-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 7, 2024 from SnapGene 7.0.3
            https://www.snapgene.com
COMMENT     Created: vrijdag 5 maart 2004 10:50
FEATURES             Location/Qualifiers
     source          1..9846
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    16..712
                     /label=mT2
                     /note="color: #00ffff"
     misc_feature    719..2919
                     /label=EPAC
                     /label=EPACdDEPCD Q270E E325T
                     /note="color: #00ff40"
     primer_bind     812..830
                     /label=MP169_FW
                     /note="color: black; sequence: TTCGAAAGCCCCCAGGTCA; added: 
                     2023-12-22"
     misc_feature    1084..1086
                     /label=Q270E
                     /note="color: #8000ff"
     misc_feature    1249..1251
                     /label=E325T
                     /note="color: #008080"
     primer_bind     1294..1317
                     /label=MP170_FW
                     /note="color: black; sequence: GGCAGGAACCGGTATACAGTGATG; 
                     added: 2023-12-18"
     primer_bind     1647..1667
                     /label=MP171_FW
                     /note="color: black; sequence: GGACACCCGACTCAGCAACCT; 
                     added: 2023-12-18"
     primer_bind     2167..2188
                     /label=MP172_FW
                     /note="color: black; sequence: CACGACTGGAGCCTCTTCAACA; 
                     added: 2023-12-18"
     misc_feature    2605..2610
                     /label=L777A / K778A
                     /note="color: #008080"
     primer_bind     2707..2726
                     /label=MP173_FW
                     /note="color: black; sequence: CACCACTGCCGAAGCCACAA; added:
                     2023-12-20"
     misc_feature    2926..4460
                     /label=cpV-cpV
                     /note="color: #ffff00"
     primer_bind     complement(4661..4683)
                     /label=GK306_RV
                     /note="color: black; sequence: CACCTACTCAGACAATGCGATGC; 
                     added: 2023-12-18"
     misc_feature    5666..6457
                     /label=Neo / Kan R
                     /note="color: #cc99ff"
     misc_feature    7965..8624
                     /label=Amp R
                     /note="color: #ffff00"
ORIGIN
        1 aagcttgccg ccaccatggc ggccgcaatg gtgagcaagg gcgaggagct gttcaccggg
       61 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      121 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
      181 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgtcctgggg cgtgcagtgc
      241 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      301 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
      361 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
      421 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actactttag cgacaacgtc
      481 tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac
      541 atcgaggacg gcggcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
      601 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct gagcaaagac
      661 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc ccgatatcag
      721 cccgtgggaa ctcatgagat ggaggaggag ttggccgaag ctgtggccct gctctcccag
      781 cgggggcctg acgccctgct cactgtggca cttcgaaagc ccccaggtca gcgcacggat
      841 gaagagctgg acctcatctt tgaggagctg ctgcacatca aggctgtggc ccacctctcc
      901 aactcggtga agcgagaatt agcggctgtt ctgctctttg aaccacacag caaggcaggg
      961 accgtgttgt tcagccaggg ggacaagggc acttcgtggt acattatctg gaagggatct
     1021 gtcaacgtgg tgacccatgg caaggggctg gtcaccaccc tgcatgaggg agatgatttt
     1081 ggagagctgg ctctggtgaa tgatgcaccc cgggcagcca ccatcatcct gcgagaagac
     1141 aactgtcatt tcctgcgtgt ggacaagcag gacttcaacc gtatcatcaa ggatgtggag
     1201 gcaaagacca tgcggctgga agaacatggc aaagtggtgc tggtgctgac gagagcctct
     1261 cagggcgccg gcccttcccg acccccaacc ccaggcagga accggtatac agtgatgtct
     1321 ggcaccccag agaagatcct agagcttctg ttggaggcca tgggaccaga ttccagtgct
     1381 catgacccaa cagagacatt cctcagcgac ttcctcctga cccacagggt cttcatgccc
     1441 agcgcccaac tctgcgctgc ccttctgcac cacttccatg tggagcctgc gggtggcagc
     1501 gagcaggagc gcagcaccta cgtctgcaac aagaggcagc agatcttgcg gctggtcagc
     1561 cagtgggtgg ccctgtatgg ctccatgctc cacactgacc ctgtggccac cagcttcctc
     1621 cagaaactct cagacctggt gggcagggac acccgactca gcaacctgct gagggagcag
     1681 tggccagaga ggcggcgatg ccacaggttg gagaatggct gtgggaatgc atctcctcag
     1741 atgaaggccc ggaacttgcc tgtttggctc cccaaccagg acgagcccct tcctggcagc
     1801 agctgtgcca tccaagttgg ggataaagtc ccctatgaca tctgccggcc agaccactca
     1861 gtgttgaccc tgcagctgcc tgtgacagcc tccgtgagag aggtgatggc agcgttggcc
     1921 caggaggatg gctggaccaa ggggcaggtg ctggtgaagg tcaattctgc aggtgatgcc
     1981 attggcctgc agccagatgc ccgtggtgtg gccacatctc tggggctcaa tgagcgtctc
     2041 tttgttgtca acccacagga agcgcatgag ctgatcccac accctgacca gctggggccc
     2101 actgtgggct ctgctgaggg gctggacctg gtgagtgcca aggacctggc aggccagctg
     2161 acggaccacg actggagcct cttcaacagt atccaccagg tggagctgat ccactatgtg
     2221 ctgggccccc agcatctgcg ggatgtcacc accgccaacc tggagcgctt catgcgccgc
     2281 ttcaatgagc tgcagtactg ggtggccacc gagctgtgtc tctgccccgt gcccggcccc
     2341 cgggcccagc tgctcaggaa gttcattaag ctggcggccc acctcaagga gcagaagaat
     2401 ctcaattcct tctttgccgt catgtttggc ctcagcaact cggccatcag ccgcctagcc
     2461 cacacctggg agcggctgcc ccacaaagtc cggaagctgt actccgccct cgagaggctg
     2521 ctggatccct catggaacca ccgggtatac cgactggccc tcgccaagct ctcccctcct
     2581 gtcatcccct tcatgcccct tcttgccgca gacatggccg ccattcatga gggaaaccac
     2641 acactagtgg agaatctcat caactttgag aagatgagaa tgatggccag agccgcgcgg
     2701 atgctgcacc actgccgaag ccacaaccct gtgcctctct caccactcag aagccgagtt
     2761 tcccacctcc acgaggacag ccaggtggcg aggatttcca catgctcgga gcagtccctg
     2821 agcacccgga gtccagccag cacctgggct tatgtccagc agctgaaggt cattgacaac
     2881 cagcgggaac tctcccgcct ctcccgagag ctggagccag ctagcgagct catggacggc
     2941 ggcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     3001 ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc caacgagaag
     3061 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     3121 gagctgtaca agggtggcag cggtggcatg gtgagcaagg gcgaggagct gttcaccggg
     3181 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     3241 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagctgat ctgcaccacc
     3301 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgggctacgg cctgcagtgc
     3361 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     3421 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     3481 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     3541 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     3601 tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac
     3661 atcgagccga attttgtctt cctgatcggc gccgcaggaa tactcttcgt atctagcgag
     3721 ctcatggacg gcggcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     3781 ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac
     3841 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     3901 ctcggcatgg acgagctgta caagggtggc agcggtggca tggtgagcaa gggcgaggag
     3961 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     4021 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagctg
     4081 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgggctac
     4141 ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     4201 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     4261 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     4321 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     4381 agccacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag
     4441 atccgccaca acatcgagcc gaattccctc gaggttaacg cggcgcgcct ctaatctaga
     4501 gggccctatt ctatagtgtc acctaaatgc tagagctcgc tgatcagcct cgactgtgcc
     4561 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     4621 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     4681 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     4741 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     4801 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     4861 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     4921 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     4981 catcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     5041 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     5101 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     5161 ctcggtctat tcttttgatt tataagggat tttggggatt tcggcctatt ggttaaaaaa
     5221 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     5281 tgtggaaagt ccccaggctc cccaggcagg cagaagtatg caaagcatgc atctcaatta
     5341 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     5401 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     5461 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     5521 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     5581 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga
     5641 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
     5701 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     5761 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     5821 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac
     5881 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     5941 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     6001 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     6061 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     6121 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     6181 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     6241 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     6301 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     6361 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     6421 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
     6481 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
     6541 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     6601 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     6661 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     6721 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     6781 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     6841 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     6901 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     6961 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     7021 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     7081 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     7141 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     7201 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     7261 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     7321 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     7381 agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     7441 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     7501 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     7561 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     7621 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     7681 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     7741 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     7801 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     7861 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     7921 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     7981 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     8041 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     8101 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     8161 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     8221 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     8281 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     8341 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     8401 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     8461 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     8521 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     8581 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     8641 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     8701 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     8761 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     8821 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     8881 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     8941 aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg tcgactctca
     9001 gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg
     9061 aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca
     9121 attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca
     9181 gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
     9241 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     9301 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     9361 cgccaatagg gactttccat tgacgtcaat gggtggacta tttacggtaa actgcccact
     9421 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     9481 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     9541 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     9601 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     9661 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     9721 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctctct
     9781 ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact cactataggg
     9841 agaccc
//
