LOCUS       pAAV-U6-SpGuideRNA(RhoP23H)-CMV-mTagBFP2_(RAS3613)        5541 bp    DNA     circular SYN 03-OCT-2024
DEFINITION  .
ACCESSION
VERSION     .
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     Annotated with pLannotate v1.2.2.
FEATURES             Location/Qualifiers
     rep_origin      1..589
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
                     /label="ori"
     repeat_region   662..689
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="19.9"
                     /fragment="True"
                     /other="repeat_region"
                     /label="AAV2 ITR (fragment)"
     repeat_region   718..745
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="19.9"
                     /fragment="True"
                     /other="repeat_region"
                     /label="AAV2 ITR (fragment)"
     protein_bind    783..800
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="72.0"
                     /fragment="True"
                     /other="protein_bind"
                     /label="attB1 (fragment)"
     promoter        852..1100
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /label="U6 promoter"
     misc_RNA        1101..1120
                     /created_by="KleinstiverLab8"
                     /label="RHO P23H spacer"
     misc_RNA        1121..1196
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_RNA"
                     /label="gRNA scaffold"
     enhancer        1209..1588
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
                     /label="CMV enhancer"
     promoter        1589..1792
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /label="CMV promoter"
     intron          1928..2060
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="intron"
                     /label="chimeric intron"
     CDS             2123..2833
                     /note="pLannotate"
                     /database="fpbase"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /label="mTagBFP2"
     misc_feature    2835..2854
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="30.3"
                     /fragment="True"
                     /other="misc_feature"
                     /label="MCS (fragment)"
     misc_feature    2883..3471
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
                     /label="WPRE"
     polyA_signal    3512..3633
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
                     /label="SV40 poly(A) signal"
     repeat_region   3704..3844
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="repeat_region"
                     /label="AAV2 ITR"
     misc_feature    complement(3849..3900)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="36.9"
                     /fragment="True"
                     /other="misc_feature"
                     /label="bom (fragment)"
     rep_origin      3919..4374
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="98.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
                     /label="f1 ori"
     misc_feature    complement(4375..4424)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="35.5"
                     /fragment="True"
                     /other="misc_feature"
                     /label="bom (fragment)"
     CDS             complement(4526..4561)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="18.8"
                     /fragment="True"
                     /other="CDS"
                     /label="rop (fragment)"
     CDS             4654..5463
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /label="KanR"
ORIGIN      
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt cctgcaggca
      661 gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt ggtcgcccgg
      721 cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact aggggttcct
      781 gctgtacaaa aaagcaggct ttaaaggaac caattcagtc gactggatcc ggtaccaagg
      841 tcgggcagga agagggccta tttcccatga ttccttcata tttgcatata cgatacaagg
      901 ctgttagaga gataattaga attaatttga ctgtaaacac aaagatatta gtacaaaata
      961 cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa
     1021 tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct
     1081 tgtggaaagg acgaaacacc gtagtactgt gggtactcga gttttagagc tagaaatagc
     1141 aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt
     1201 tttctagcga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
     1261 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
     1321 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     1381 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     1441 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     1501 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     1561 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
     1621 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
     1681 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
     1741 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt
     1801 gaaccgtcag atcactagaa gctttattgc ggtagtttat cacagttaaa ttgctaacgc
     1861 agtcagtgct tctgacacaa cagtctcgaa cttaagctgc agaagttggt cgtgaggcac
     1921 tgggcaggta agtatcaagg ttacaagaca ggtttaagga gaccaataga aactgggctt
     1981 gtcgagacag agaagactct tgcgtttctg ataggcacct attggtctta ctgacatcca
     2041 ctttgccttt ctctccacag gtgtccactc ccagttcaat tacagctctt aaggctagag
     2101 tacttaatta accggtgcca ccatggtgtc taagggcgaa gagctgatta aggagaacat
     2161 gcacatgaag ctgtacatgg agggcaccgt ggacaaccat cacttcaagt gcacatccga
     2221 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggtgg tcgagggcgg
     2281 ccctctcccc ttcgccttcg acatcctggc tactagcttc ctctacggca gcaagacctt
     2341 catcaaccac acccagggca tccccgactt cttcaagcag tccttccctg agggcttcac
     2401 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
     2461 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcacatccaa
     2521 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc ttcaccgaga cgctgtaccc
     2581 cgctgacggc ggcctggaag gcagaaacga catggccctg aagctcgtgg gcgggagcca
     2641 tctgatcgca aacgccaaga ccacatatag atccaagaaa cccgctaaga acctcaagat
     2701 gcctggcgtc tactatgtgg actacagact ggaaagaatc aaggaggcca acaacgagac
     2761 ctacgtcgag cagcacgagg tggcagtggc cagatactgc gacctcccta gcaaactggg
     2821 gcacaaactt aattactcag atctcgagct caagtagctt aagaattcat cagcttatcg
     2881 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     2941 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     3001 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     3061 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     3121 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     3181 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     3241 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     3301 tcgcctatgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     3361 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     3421 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc
     3481 gagcgctcta gagctcgctg atcagcctcg aaacttgttt attgcagctt ataatggtta
     3541 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     3601 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccag caggctttcc
     3661 taggttctca cgtgcggacc gaggctgcag cgtcgtcctc cctaggaacc cctagtgatg
     3721 gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc
     3781 gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg
     3841 caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     3901 cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     3961 ttacgcgcag cgtgaccgct acacttgcca gcgccttagc gcccgctcct ttcgctttct
     4021 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     4081 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg
     4141 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     4201 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac tctatctcgg
     4261 gctattcttt tgatttataa gggattttgc cgatttcggt ctattggtta aaaaatgagc
     4321 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt
     4381 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     4441 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     4501 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     4561 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgaac aataaaactg
     4621 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg
     4681 aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat
     4741 aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag
     4801 ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga
     4861 ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct
     4921 gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa
     4981 gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg
     5041 cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag
     5101 gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat
     5161 ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat
     5221 tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta
     5281 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc
     5341 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat
     5401 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc
     5461 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     5521 agaaaagatc aaaggatctt c
//
