LOCUS       Exported               11859 bp ds-DNA     circular SYN 08-OCT-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11859)
  AUTHORS   Herr Michael Boettcher
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Oct 8, 2024 from SnapGene 5.1.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11859
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     CDS             282..1088
                     /label=AmpR
                     /note="AmpR"
     rep_origin      complement(1214..1829)
                     /direction=LEFT
                     /label=pUC Ori
                     /note="pUC Ori"
     LTR             2177..2589
                     /label=5' LTR
                     /note="5' LTR"
                     /note="RSV/5'LTR"
     misc_feature    2640..2777
                     /label=psi
                     /note="psi"
     misc_feature    2744..3108
                     /label=gag
                     /note="gag"
     misc_feature    3254..3495
                     /label=RRE
                     /note="RRE"
     misc_feature    3896..4013
                     /label=cPPT
                     /note="cPPT"
     polyA_signal    4063..4193
                     /label=SV40 polyA
                     /note="SV40 polyA"
     misc_feature    4204..4236
                     /label=TruSeq PBS
     misc_binding    4243..4420
                     /label=filler (1,885 bp)
                     /note="filler (1,885 bp)"
                     /note="Filler (digest with BsmBI to replace fillerwithsgRNA
                     oligos)"
     misc_feature    4244..4249
                     /label=BsmBI
                     /label=BsmBI(1)
                     /note="BsmBI"
                     /note="Geneious type: restriction site"
     misc_binding    4421..4542
                     /label=filler (1,885 bp)
                     /note="filler (1,885 bp)"
                     /note="Filler (digest with BsmBI to replace fillerwithsgRNA
                     oligos)"
     misc_feature    4536..4541
                     /label=BsmBI
                     /note="BsmBI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(4543..4857)
                     /label=mouse U6 promoter
                     /note="mouse U6 promoter"
     misc_feature    4926..4927
                     /label=origin
                     /note="origin"
     misc_feature    4927..4929
                     /label=SmaI site
     promoter        4930..5185
                     /label=EFS-NS
                     /note="EFS-NS"
     CDS             5209..5235
                     /codon_start=1
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             5236..5262
                     /codon_start=1
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             5263..5289
                     /codon_start=1
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             5290..9207
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcussp.
                     BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="TQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDH
                     YKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRNA
                     IHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFDK
                     FTTYFSGFYRNRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHFE
                     NVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVLN
                     LAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLR
                     NENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTGK
                     ITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLK
                     KQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKAR
                     NYATKKPYSVEKFKLNFQMPTLARGWDVNREKNNGAILFVKNGLYYLGIMPKQKGRYKA
                     LSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEPL
                     EITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDL
                     SSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGH
                     HGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKD
                     QKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFFH
                     VPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQRS
                     LNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAVV
                     VLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQF
                     TSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDV
                     KTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIENH
                     RFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQMR
                     NSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKESK
                     DLKLQNGISNQDWLAYIQELRN"
     regulatory      8927..8936
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong 
                     initiationof translation (Kozak, 1987)"
     CDS             9219..9279
                     /codon_start=1
                     /label=P2A
                     /note="P2A"
                     /translation="RRNKLLSAETSRRCRRESWT"
     CDS             9280..9876
                     /label=Puro
                     /note="Puro"
                     /note="Puromycin resistance"
     misc_feature    9411..9417
                     /label=BstEII site
     misc_RNA        9892..10480
                     /label=WPRE
                     /note="WPRE"
     LTR             10551..10786
                     /label=3' LTR
     polyA_signal    10863..10993
                     /label=SV40 polyA
                     /note="SV40 polyA"
ORIGIN
        1 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
       61 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
      121 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
      181 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
      241 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
      301 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
      361 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
      421 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
      481 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
      541 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
      601 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
      661 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
      721 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
      781 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
      841 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
      901 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
      961 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     1021 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     1081 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     1141 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     1201 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     1261 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     1321 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     1381 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     1441 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     1501 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     1561 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     1621 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     1681 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     1741 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     1801 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     1861 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     1921 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     1981 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     2041 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     2101 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
     2161 agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt
     2221 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt
     2281 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat
     2341 tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata
     2401 cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     2461 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     2521 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     2581 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     2641 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     2701 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     2761 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     2821 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     2881 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     2941 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     3001 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     3061 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     3121 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     3181 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     3241 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     3301 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     3361 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     3421 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     3481 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     3541 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     3601 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     3661 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     3721 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     3781 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     3841 atattcacca ttatcgtttc agaatcttga gacaaatggc agtattcatc cacaatttta
     3901 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     3961 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
     4021 attacaggga cagcagagat ccactttggc gccggcctcg agcagacatg ataagataca
     4081 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     4141 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttggatttg
     4201 gatgtgactg gagttcagac gtgtgctctt ccgatcaaaa aaagagacgt acaaaaaaga
     4261 gcaagaagct aaaaaagatt taaaaattat ttttagcgca gttaatggaa caggaactaa
     4321 atttacccca aagaatcagg atatgaagat gaaacattta aaaatgttgt aaatccaaat
     4381 ccagaatttg atcctgcatg aaaaataccg cttgaatatg tttattttgc tcatttttat
     4441 taaaagtttg caaaaaggaa cataaaaatt ctaattattg atactaaagt tattaaaaag
     4501 aagggtcata gaatataata ttgctcattt acaaccgtct ccccaacaag gtggttctcc
     4561 aagggatact tatagtctca aaacacacaa ttactttaca gttagggtga gtttcctttt
     4621 gtgctgttta taacttcgta taatgtatgc tatacgaagt tatagaaatc caagcctatc
     4681 atgtaaaatg tagctagtat taaaaagaac agattatctg tcttttatcg cacattaagc
     4741 ctctatagtt actaggaaat attatatgca aattaaccgg ggcaggggag tagccgagct
     4801 tctcccacaa gtccgtgcga gggggccggc gcgggcctag agatggcgcg tcggatcagc
     4861 gctagaacaa tggtccatat aactccagtt attgaggttg aagagcatgc ccgggctcga
     4921 ggggggccct aggtcttgaa aggagtggga attggctccg gtgcccgtca gtgggcagag
     4981 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg atccggtgcc
     5041 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     5101 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     5161 aacgggtttg ccgccagaac acaggaccgg ttctagacta gagcgatgcc tgctgctaag
     5221 agagtgaaac tggatcctgc tgctaagaga gtgaaactgg atcctgctgc taagagagtg
     5281 aaactggata cacagttcga gggctttacc aacctgtatc aggtgagcaa gacactgcgg
     5341 tttgagctga tcccacaggg caagaccctg aagcacatcc aggagcaggg cttcatcgag
     5401 gaggacaagg cccgcaatga tcactacaag gagctgaagc ccatcatcga tcggatctac
     5461 aagacctatg ccgaccagtg cctgcagctg gtgcagctgg attgggagaa cctgagcgcc
     5521 gccatcgact cctatagaaa ggagaaaacc gaggagacaa ggaacgccct gatcgaggag
     5581 caggccacat atcgcaatgc catccacgac tacttcatcg gccggacaga caacctgacc
     5641 gatgccatca ataagagaca cgccgagatc tacaagggcc tgttcaaggc cgagctgttt
     5701 aatggcaagg tgctgaagca gctgggcacc gtgaccacaa ccgagcacga gaacgccctg
     5761 ctgcggagct tcgacaagtt tacaacctac ttctccggct tttatagaaa caggaagaac
     5821 gtgttcagcg ccgaggatat cagcacagcc atcccacacc gcatcgtgca ggacaacttc
     5881 cccaagttta aggagaattg tcacatcttc acacgcctga tcaccgccgt gcccagcctg
     5941 cgggagcact ttgagaacgt gaagaaggcc atcggcatct tcgtgagcac ctccatcgag
     6001 gaggtgtttt ccttcccttt ttataaccag ctgctgacac agacccagat cgacctgtat
     6061 aaccagctgc tgggaggaat ctctcgggag gcaggcaccg agaagatcaa gggcctgaac
     6121 gaggtgctga atctggccat ccagaagaat gatgagacag cccacatcat cgcctccctg
     6181 ccacacagat tcatccccct gtttaagcag atcctgtccg ataggaacac cctgtctttc
     6241 atcctggagg agtttaagag cgacgaggaa gtgatccagt ccttctgcaa gtacaagaca
     6301 ctgctgagaa acgagaacgt gctggagaca gccgaggccc tgtttaacga gctgaacagc
     6361 atcgacctga cacacatctt catcagccac aagaagctgg agacaatcag cagcgccctg
     6421 tgcgaccact gggatacact gaggaatgcc ctgtatgagc ggagaatctc cgagctgaca
     6481 ggcaagatca ccaagtctgc caaggagaag gtgcagcgca gcctgaagca cgaggatatc
     6541 aacctgcagg agatcatctc tgccgcaggc aaggagctga gcgaggcctt caagcagaaa
     6601 accagcgaga tcctgtccca cgcacacgcc gccctggatc agccactgcc tacaaccctg
     6661 aagaagcagg aggagaagga gatcctgaag tctcagctgg acagcctgct gggcctgtac
     6721 cacctgctgg actggtttgc cgtggatgag tccaacgagg tggaccccga gttctctgcc
     6781 cggctgaccg gcatcaagct ggagatggag ccttctctga gcttctacaa caaggccaga
     6841 aattatgcca ccaagaagcc ctactccgtg gagaagttca agctgaactt tcagatgcct
     6901 acactggcca gaggctggga cgtgaataga gagaagaaca atggcgccat cctgtttgtg
     6961 aagaacggcc tgtactatct gggcatcatg ccaaagcaga agggcaggta taaggccctg
     7021 agcttcgagc ccacagagaa aaccagcgag ggctttgata agatgtacta tgactacttc
     7081 cctgatgccg ccaagatgat cccaaagtgc agcacccagc tgaaggccgt gacagcccac
     7141 tttcagaccc acacaacccc catcctgctg tccaacaatt tcatcgagcc tctggagatc
     7201 acaaaggaga tctacgacct gaacaatcct gagaaggagc caaagaagtt tcagacagcc
     7261 tacgccaaga aaaccggcga ccagaagggc tacagagagg ccctgtgcaa gtggatcgac
     7321 ttcacaaggg attttctgtc caagtatacc aagacaacct ctatcgatct gtctagcctg
     7381 cggccatcct ctcagtataa ggacctgggc gagtactatg ccgagctgaa tcccctgctg
     7441 taccacatca gcttccagag aatcgccgag aaggagatca tggatgccgt ggagacaggc
     7501 aagctgtacc tgttccagat ctataacaag gactttgcca agggccacca cggcaagcct
     7561 aatctgcaca cactgtattg gaccggcctg ttttctccag agaacctggc caagacaagc
     7621 atcaagctga atggccaggc cgagctgttc taccgcccta agtccaggat gaagaggatg
     7681 gcacaccggc tgggagagaa gatgctgaac aagaagctga aggatcagaa aaccccaatc
     7741 cccgacaccc tgtaccagga gctgtacgac tatgtgaatc acagactgtc ccacgacctg
     7801 tctgatgagg ccagggccct gctgcccaac gtgatcacca aggaggtgtc tcacgagatc
     7861 atcaaggata ggcgctttac cagcgacaag ttctttttcc acgtgcctat cacactgaac
     7921 tatcaggccg ccaattcccc atctaagttc aaccagaggg tgaatgccta cctgaaggag
     7981 caccccgaga cacctatcat cggcatcgat cggggcgaga gaaacctgat ctatatcaca
     8041 gtgatcgact ccaccggcaa gatcctggag cagcggagcc tgaacaccat ccagcagttt
     8101 gattaccaga agaagctgga caacagggag aaggagaggg tggcagcaag gcaggcctgg
     8161 tctgtggtgg gcacaatcaa ggatctgaag cagggctatc tgagccaggt catccacgag
     8221 atcgtggacc tgatgatcca ctaccaggcc gtggtggtgc tggagaacct gaatttcggc
     8281 tttaagagca agaggaccgg catcgccgag aaggccgtgt accagcagtt cgagaagatg
     8341 ctgatcgata agctgaattg cctggtgctg aaggactatc cagcagagaa agtgggaggc
     8401 gtgctgaacc cataccagct gacagaccag ttcacctcct ttgccaagat gggcacccag
     8461 tctggcttcc tgttttacgt gcctgcccca tatacatcta agatcgatcc cctgaccggc
     8521 ttcgtggacc ccttcgtgtg gaaaaccatc aagaatcacg agagccgcaa gcacttcctg
     8581 gagggcttcg actttctgca ctacgacgtg aaaaccggcg acttcatcct gcactttaag
     8641 atgaacagaa atctgtcctt ccagaggggc ctgcccggct ttatgcctgc atgggatatc
     8701 gtgttcgaga agaacgagac acagtttgac gccaagggca cccctttcat cgccggcaag
     8761 agaatcgtgc cagtgatcga gaatcacaga ttcaccggca gataccggga cctgtatcct
     8821 gccaacgagc tgatcgccct gctggaggag aagggcatcg tgttcaggga tggctccaac
     8881 atcctgccaa agctgctgga gaatgacgat tctcacgcca tcgacaccat ggtggccctg
     8941 atccgcagcg tgctgcagat gcggaactcc aatgccgcca caggcgagga ctatatcaac
     9001 agccccgtgc gcgatctgaa tggcgtgtgc ttcgactccc ggtttcagaa cccagagtgg
     9061 cccatggacg ccgatgccaa tggcgcctac cacatcgccc tgaagggcca gctgctgctg
     9121 aatcacctga aggagagcaa ggatctgaag ctgcagaacg gcatctccaa tcaggactgg
     9181 ctggcctaca tccaggagct gcgcaacgct agcggatccg gcgcaacaaa cttctctctg
     9241 ctgaaacaag ccggagatgt cgaagagaat cctggaccga ccgagtacaa gcccacggtg
     9301 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
     9361 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
     9421 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     9481 gacggcgccg ccgtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     9541 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     9601 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     9661 ggagtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     9721 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     9781 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
     9841 cgcacctggt gcatgacccg caagcccggt gcctgaacgc gttaagtcga caatcaacct
     9901 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     9961 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
    10021 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
    10081 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
    10141 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
    10201 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
    10261 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
    10321 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
    10381 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
    10441 cctcagacga gtcggatctc cctttgggcc gcctccccgc gtcgacttta agaccaatga
    10501 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
    10561 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
    10621 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
    10681 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
    10741 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt
    10801 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
    10861 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
    10921 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
    10981 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
    11041 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
    11101 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
    11161 agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
    11221 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
    11281 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
    11341 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
    11401 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
    11461 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
    11521 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
    11581 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
    11641 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
    11701 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
    11761 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
    11821 taggtggcac ttttcgggga aatgtgcgcg gaaccccta
//
