LOCUS       pACE_MBP_FBXL12_        6256 bp    DNA        circular     01-OCT-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  . .
COMMENT     Annotated with pLannotate v1.2.0
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     enhancer        1067..1446
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
                     /locus_tag="CMV enhancer"
                     /label="CMV enhancer"
                     /ApEinfo_label="CMV enhancer"
                     /ApEinfo_fwdcolor="#00DF87"
                     /ApEinfo_revcolor="#00DF87"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        1448..1647
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /locus_tag="CMV promoter"
                     /label="CMV promoter"
                     /ApEinfo_label="CMV promoter"
                     /ApEinfo_fwdcolor="#00D95A"
                     /ApEinfo_revcolor="#00D95A"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     mobile_element  complement(5023..5188)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="mobile_element"
                     /locus_tag="Tn7L"
                     /label="Tn7L"
                     /ApEinfo_label="Tn7L"
                     /ApEinfo_fwdcolor="#008065"
                     /ApEinfo_revcolor="#008065"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     polyA_signal    4526..4660
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
                     /locus_tag="SV40 poly(A) signal"
                     /label="SV40 poly(A) signal"
                     /ApEinfo_label="SV40 poly(A) signal"
                     /ApEinfo_fwdcolor="#13AAFE"
                     /ApEinfo_revcolor="#13AAFE"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     promoter        1687..1796
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /locus_tag="p10 promoter"
                     /label="p10 promoter"
                     /ApEinfo_label="p10 promoter"
                     /ApEinfo_fwdcolor="#3A93FE"
                     /ApEinfo_revcolor="#3A93FE"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             2007..3104
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="97.0"
                     /match_length="99.7"
                     /fragment="False"
                     /other="CDS"
                     /locus_tag="MBP"
                     /label="MBP"
                     /ApEinfo_label="MBP"
                     /ApEinfo_fwdcolor="#2D64FE"
                     /ApEinfo_revcolor="#2D64FE"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      5378..5966
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
                     /locus_tag="ori"
                     /label="ori"
                     /ApEinfo_label="ori"
                     /ApEinfo_fwdcolor="#00891F"
                     /ApEinfo_revcolor="#00891F"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     CDS             complement(135..668)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /locus_tag="GmR"
                     /label="GmR"
                     /ApEinfo_label="GmR"
                     /ApEinfo_fwdcolor="#00C6C3"
                     /ApEinfo_revcolor="#00C6C3"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             3042..4325
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="91.5"
                     /match_length="61.1"
                     /fragment="True"
                     /other="CDS"
                     /locus_tag="Fbxl17 (fragment)"
                     /label="Fbxl17 (fragment)"
                     /ApEinfo_label="Fbxl17 (fragment)"
                     /ApEinfo_fwdcolor="#00D0FA"
                     /ApEinfo_revcolor="#00D0FA"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    956..989
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
                     /locus_tag="loxP"
                     /label="loxP"
                     /ApEinfo_label="loxP"
                     /ApEinfo_fwdcolor="#00D308"
                     /ApEinfo_revcolor="#00D308"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     promoter        complement(857..885)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /locus_tag="Pc promoter"
                     /label="Pc promoter"
                     /ApEinfo_label="Pc promoter"
                     /ApEinfo_fwdcolor="#2D64FE"
                     /ApEinfo_revcolor="#2D64FE"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     mobile_element  complement(join(6101..6256,1..68))
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="100.0"
                     /fragment="False"
                     /other="mobile_element"
                     /locus_tag="Tn7R"
                     /label="Tn7R"
                     /ApEinfo_label="Tn7R"
                     /ApEinfo_fwdcolor="#EE92FE"
                     /ApEinfo_revcolor="#EE92FE"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     CDS             4776..4994
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="97.3"
                     /match_length="38.8"
                     /fragment="True"
                     /other="CDS"
                     /locus_tag="KRAS (fragment)"
                     /label="KRAS (fragment)"
                     /ApEinfo_label="KRAS (fragment)"
                     /ApEinfo_fwdcolor="#A600FA"
                     /ApEinfo_revcolor="#A600FA"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             4689..4738
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="39.7"
                     /fragment="True"
                     /other="CDS"
                     /locus_tag="SET1 (fragment)"
                     /label="SET1 (fragment)"
                     /ApEinfo_label="SET1 (fragment)"
                     /ApEinfo_fwdcolor="#4856FE"
                     /ApEinfo_revcolor="#4856FE"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     intron          1806..1838
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="34.4"
                     /fragment="True"
                     /other="intron"
                     /locus_tag="CMV intron (fragment)"
                     /label="CMV intron (fragment)"
                     /ApEinfo_label="CMV intron (fragment)"
                     /ApEinfo_fwdcolor="#FE09C9"
                     /ApEinfo_revcolor="#FE09C9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     CDS             1977..2000
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="95.8"
                     /match_length="33.3"
                     /fragment="True"
                     /other="CDS"
                     /locus_tag="3xFLAG (fragment)"
                     /label="3xFLAG (fragment)"
                     /ApEinfo_label="3xFLAG (fragment)"
                     /ApEinfo_fwdcolor="#FE139F"
                     /ApEinfo_revcolor="#FE139F"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             4420..4503
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="3.9"
                     /fragment="True"
                     /other="CDS"
                     /locus_tag="large T antigen (fragment)"
                     /label="large T antigen (fragment)"
                     /ApEinfo_label="large T antigen (fragment)"
                     /ApEinfo_fwdcolor="#CB00C4"
                     /ApEinfo_revcolor="#CB00C4"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 tttctgaagt gcaaattgcc cgtcgtatta aagaggggcg tggccaaggg catgtaaaga
       61 ctatattcgc ggcgttgtga caatttaccg aacaactccg cggccgggaa gccgatctcg
      121 gcttgaacga attgttaggt ggcggtactt gggtcgatat caaagtgcat cacttcttcc
      181 cgtatgccca actttgtata gagagccact gcgggatcgt caccgtaatc tgcttgcacg
      241 tagatcacat aagcaccaag cgcgttggcc tcatgcttga ggagattgat gagcgcggtg
      301 gcaatgccct gcctccggtg ctcgccggag actgcgagat catagatata gatctcacta
      361 cgcggctgct caaacttggg cagaacgtaa gccgcgagag cgccaacaac cgcttcttgg
      421 tcgaaggcag caagcgcgat gaatgtctta ctacggagca agttcccgag gtaatcggag
      481 tccggctgat gttgggagta ggtggctacg tctccgaact cacgaccgaa aagatcaaga
      541 gcagcccgca tggatttgac ttggtcaggg ccgagcctac atgtgcgaat gatgcccata
      601 cttgagccac ctaactttgt tttagggcga ctgccctgct gcgtaacatc gttgctgctg
      661 cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc acggcgtaac gcgcttgctg
      721 cttggatgcc cgaggcatag actgtacaaa aaaacagtca taacaagcca tgaaaaccgc
      781 cactgcgccg ttaccaccgc tgcgttcggt caaggttctg gaccagttgc gtgagcgcat
      841 acgctacttg cattacagtt tacgaaccga acaggcttat gtcaactggg ttcgtgcctt
      901 catccgtttc cacggtgtgc gtcacccggc aaccttgggc agcagcgaag tcgccataac
      961 ttcgtatagc atacattata cgaagttatc tgtaactata acggtcctaa ggtagcgagt
     1021 ttaaacacta gtatcgatcg cgatgtacgg gccagatata cgcgttgaca ttgattattg
     1081 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     1141 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     1201 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     1261 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     1321 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     1381 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     1441 accatggtga tgcggttttg gcagtacacc aatgggcgtg gatagcggtt tgactcacgg
     1501 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
     1561 cgggactttc caaaatgtcg taataacccc gccccgttga cgcaaatggg cggtaggcgt
     1621 gtacggtggg aggtctatat aagcagacgt cgtttagtga accgtcagat caccatccag
     1681 tatacggacc tttaattcaa cccaacacaa tatattatag ttaaataaga attattatca
     1741 aatcatttgt atattaatta aaatactata ctgtaaatta cattttattt acaatcactc
     1801 gacagacaga ctgttccttt cctgggtctt ttctgcaggc accgtcgtcg atttaacaga
     1861 tctgtttgga tccttaagaa ggagatatac tggattattc ataccgtccc accatcgggc
     1921 gcggatctcg gtccgaaacc atgtcgtact accaccacca tcaccatcac tccggtgact
     1981 acaaagacga tgacgataaa ggtaccaaaa ccgaagaagg taaactggta atctggatta
     2041 acggcgataa aggctataac ggtctggctg aagtcggtaa gaaattcgag aaagataccg
     2101 gaattaaagt caccgttgag catcccgata aactggaaga gaaattccca caggttgcgg
     2161 caactggcga tggccctgac attatcttct gggcacacga ccgctttggt ggctacgctc
     2221 aatctggcct gttggctgaa atcaccccgg ccgcagcgtt ccaggacaag ctgtatccgt
     2281 ttacctggga tgccgtgcgc tacaacggca agctgattgc ttacccgatc gctgttgaag
     2341 cgttgtccct gatttataac aaggatctgc tgccgaaccc gccaaaaacc tgggaagaga
     2401 tcccggcgct ggataaagaa ctgaaagcga aaggtaagag cgcgctgatg ttcaacctgc
     2461 aagaaccgta cttcacctgg ccgctgattg ctgctgacgg gggttatgcg ttcaagtatg
     2521 cagccggaaa gtacgacatt aaagacgtgg gcgtggataa cgctggcgcg aaagcgggtc
     2581 tgaccttcct ggttgacctg attaaaaaca aacacatgaa tgcagacacc gattactcca
     2641 tcgcagaaca tgcctttaat catggcgaaa cagcgatgac catcaacggc ccgtgggcat
     2701 ggtccaacat cgacaccagc gcagtgaatt atggtgtaac ggtgctgccg accttcaagg
     2761 gtcaaccatc caaaccgttc gttggcgtgc tgagcgcagg tattaacgcc gccagtccga
     2821 acaaagagct ggcaaaagag ttcctcgaaa actacctgct gactgatgaa ggtctggaag
     2881 cggttaataa agacaaaccg ctgggtgccg tagcgctgaa gtcttacgag gaagagttgg
     2941 tgaaagatcc acgtgttgcc gccacaatgg aaaacgccca gaaaggtgaa atcatgccca
     3001 acatcccgca gatgtccgct ttctggtatg ccgtgcgcac tgcggtgatt aacgccgcca
     3061 gcggtcgtca gactgtcgat gcagccctgg cagccgcgca gactaatgct gctagcggtt
     3121 ctgtcgacga aaacctctac ttccaatcgt gccaccgcga accaccaccc gagacaccag
     3181 atataaatca gctccccccg tctatacttt tgaagatctt ttcgaatctt tcacttgatg
     3241 agcgttgcct ttctgcgtcg ctggtttgta agtactggcg cgatctctgt ctagattttc
     3301 agttctggaa gcagctcgac ctctctagta ggcaacaggt cacggatgag ctattagaaa
     3361 aaatcgcttc cagatcgcaa aatattatcg agataaacat atccgactgc aggtccatga
     3421 gcgataacgg tgtttgcgtc ctagccttca aatgtcctgg cttattacga tacacagcct
     3481 atcggtgtaa acaactgagc gacacatcga ttatcgcagt tgcctcccac tgtcctctac
     3541 tacaaaaagt tcacgtgggt aaccaagata agctcacaga cgagggacta aagcaattgg
     3601 gcagcaagtg tcgggaattg aaagatatcc attttggaca gtgctacaag atctcggacg
     3661 agggaatgat tgtaatagct aagggctgtc ttaaattgca aaggatatat atgcaggaga
     3721 acaaattagt aactgaccag tccgtaaagg cattcgccga acattgcccg gaactccaat
     3781 atgtgggctt tatgggatgt tcagttacca gcaagggtgt aattcatctg acgaagctgc
     3841 gtaatctgtc ctcactcgat ttgcgacata ttacggagtt agataatgaa actgtaatgg
     3901 aaattgtgaa gcgttgcaaa aacctctcat ctctgaactt gtgtcttaat tggatcataa
     3961 atgacagatg cgtcgaagtg atcgctaagg aaggtcagaa cttgaaagag ctatacttag
     4021 tgagctgcaa gattactgat tatgcactta tcgcgatagg gcgctatagc atgaccattg
     4081 aaaccgtaga cgtggggtgg tgtaaagaga taacggacca gggggccact ctaattgcac
     4141 agagttctaa atctttacga tacctgggtt tgatgcgatg tgacaaagtt aatgaggtca
     4201 cggtcgaaca gcttgtccaa caatatcctc acattacctt cagtaccgta ttacaagact
     4261 gcaaaagaac actggaacgg gcgtaccaaa tggggtggac tccgaacatg agtgcggcta
     4321 gttcataacc ggaggatgag tgtggcctga gtctcgaggc atgggtacag gttcctctag
     4381 agcctgcagt ctcgacaagc ttgtcgagaa gtactagagg atcataatca gccataccac
     4441 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     4501 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     4561 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     4621 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc tgatcactgc ttgagcctag
     4681 aagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc
     4741 aataactatc ataaccccta gaggccgcgc tggccaaact tgtggtagtt ggagctgacg
     4801 gcgtaggcaa gagtgccttg acgatccagc tgattcagaa tcattttgtg gacgaatatg
     4861 atccaaccat cgaggattcc taccgtaagc aagtagtaat tgatggcgaa acctgtctct
     4921 tggatattct cgacacagca ggtcaagagg agtacagtgc aatgcgtgac cagtacatgc
     4981 gtactggcct gggcggcccc tagggtatac ccatctaatt ggaaccagat aagtgaaatc
     5041 tagttccaaa ctattttgtc atttttaatt ttcgtattag cttacgacgc tacacccagt
     5101 tcccatctat tttgtcactc ttccctaaat aatccttaaa aactccattt ccacccctcc
     5161 cagttcccaa ctattttgtc cgcccacaac cggttgactt gggtcaactg tcagaccaag
     5221 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     5281 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     5341 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     5401 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     5461 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     5521 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     5581 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     5641 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     5701 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     5761 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     5821 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     5881 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     5941 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     6001 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tgacttgggt
     6061 cgctcttcct gtggatgcgc agatgccctg cgtaagcggg tgtgggcgga caataaagtc
     6121 ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt
     6181 tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct
     6241 aaagcaaact cttcat    
//
