LOCUS       MZB211        8144 bp DNA     circular SYN 09-MAR-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 12, 2024 from SnapGene 7.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8144
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     misc_feature    208..226
                     /label=T7
                     /note="/vntifkey=21"
     protein_bind    227..251
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             265..287
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             301..330
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     misc_feature    337..630
                     /label=Smt3 (SUMO)
                     /label=MZB-SUMO
     primer_bind     537..559
                     /label=Primer 2
     CDS             631..2955
                     /codon_start=1
                     /label=pol
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MILDVDYITEEGKPVIRLFKKENGKFKIEHDRTFRPYIYALLRDD
                     SKIEEVKKITGERHGKIVRIVDVEKVEKKFLGKPITVWKLYLEHPQDVPTIREKVREHP
                     AVVDIFEYDIPFAKRYLIDKGLIPMEGEEELKILAFDIETLYHEGEEFGKGPIIMISYA
                     DENEAKVITWKNIDLPYVEVVSSEREMIKRFLRIIREKDPDIIVTYNGDSFDFPYLAKR
                     AEKLGIKLTIGRDGSEPKMQRIGDMTAVEVKGRIHFDLYHVITRTINLPTYTLEAVYEA
                     IFGKPKEKVYADEIAKAWESGENLERVAKYSMEDAKATYELGKEFLPMEIQLSRLVGQP
                     LWDVSRSSTGNLVEWFLLRKAYERNEVAPNKPSEEEYQRRLRESYTGGFVKEPEKGLWE
                     NIVYLDFRSLYPSIIITHNVSPDTLNLEGCKNYDIAPQVGHKFCKDIPGFIPSLLGHLL
                     EERQKIKTKMKETQDPIEKILLDYRQKAIKLLANSFYGYYGYAKARWYCKECAESVTAW
                     GRKYIELVWKELEEKFGFKVLYIDTDGLYATIPGGESEEIKKKALEFVKYINSKLPGLL
                     ELEYEGFYKRGFFVTKKRYAVIDEEGKVITRGLEIVRRDWSEIAKETQARVLETILKHG
                     DVEEAVRIVKEVIQKLANYEIPPEKLAIYEQITRPLHEYKAIGPHVAVAKKLAAKGVKI
                     KPGMVIGYIVLRGDGPISNRAILAEEYDPKKHKYDAEYYIENQVLPAVLRILEGFGYRK
                     EDLRYQKTRQVGLTSWLNIKKS"
     primer_bind     complement(1824..1861)
                     /label=Primer 1
     CDS             2974..3162
                     /codon_start=1
                     /label=sso7d
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=98.4"
                     /note="/other=CDS"
                     /translation="ATVKFKYKGEEKEVDISKIKKVWRVGKMISFTYDEGGGKTGRGAV
                     SEKDAPKELLQMLEKQKK"
     CDS             3255..3272
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      3338..3385
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      3421..3873
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     misc_feature    complement(3964..4773)
                     /label=KanR2
                     /note="/vntifkey=21"
     misc_feature    complement(4878..5492)
                     /label=PBR322 Origin
                     /label=PBR322_Origin
                     /note="/vntifkey=21"
     rep_origin      4894..5477
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    5662..5800
                     /label=bom
                     /note="basis of mobility region from pBR322"
     misc_feature    complement(5901..6091)
                     /label=ROP
                     /note="/vntifkey=21"
     misc_feature    complement(6562..6835)
                     /label=tet
                     /note="/vntifkey=21"
     misc_feature    complement(6893..7976)
                     /label=lacI
                     /note="/vntifkey=21"
     promoter        complement(7968..8044)
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa
       61 acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat
      121 ataggcgcag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag
      181 aggatcgaga tctcgatccc gcgaaattaa tacgactcac tataggggaa ttgtgagcgg
      241 ataacaattc cccttagaaa taattttgtt taactttaag aaggagatat acatatgggc
      301 catcaccatc accatcacca tcaccatcac ggcagcatga gcgatagcga agtgaaccag
      361 gaagcgaaac cggaagtgaa accggaagtg aaaccggaaa cccatattaa cctgaaagtg
      421 agcgatggca gcagcgaaat tttttttaaa attaaaaaaa ccaccccgct gcgtcgtctg
      481 atggaagcgt ttgcgaaacg tcagggcaaa gaaatggata gcctgcgttt tctgtatgat
      541 ggcattcgta ttcaggcgga tcagaccccg gaagatctgg atatggaaga taacgatatt
      601 attgaagcgc atcgtgaaca gattggcggc atgattctgg acgttgatta cattactgag
      661 gaaggcaaac cggttatccg cctgtttaag aaagaaaacg gtaaattcaa aatcgaacat
      721 gaccgtactt tccgcccgta catctacgca ctgctgcgtg acgactctaa gatcgaagag
      781 gttaagaaaa tcaccggtga acgtcacggt aaaatcgttc gcattgtaga tgtggaaaag
      841 gtagaaaaga agttcctggg taaaccaatc actgtttgga aactgtacct ggaacacccg
      901 caggacgtac caactattcg tgaaaaggta cgtgagcacc cggcggtggt tgacattttc
      961 gaatacgaca tcccgtttgc gaaacgttat ctgatcgaca agggtctgat cccaatggaa
     1021 ggcgaagaag agctgaagat cctggcattc gatatcgaga ccctgtacca cgaaggtgag
     1081 gaattcggca aaggtccgat catcatgatc tcctacgcgg atgaaaacga agcgaaggta
     1141 attacttgga agaacatcga cctgccgtac gttgaagttg taagctctga acgtgaaatg
     1201 attaaacgtt tcctgcgtat catccgtgaa aaggatccgg acatcattgt aacttacaac
     1261 ggcgactctt tcgatttccc gtacctggca aaacgtgctg agaaactggg cattaaactg
     1321 accatcggtc gtgatggctc cgaaccgaaa atgcagcgta tcggtgacat gaccgcggta
     1381 gaggttaagg gtcgtatcca ttttgatctg tatcacgtaa tcactcgtac catcaacctg
     1441 ccgacctaca ctctggaagc agtttacgaa gcaatcttcg gtaaaccgaa ggagaaagta
     1501 tacgcagacg aaattgcgaa agcatgggag tctggtgaaa acctggagcg tgtagcaaaa
     1561 tactctatgg aagacgcgaa agcaacttat gaactgggta aggaattcct gccgatggaa
     1621 atccagctga gccgcctggt tggccagccg ctgtgggacg tttcccgttc ttccaccggt
     1681 aacctggttg aatggttcct gctgcgtaaa gcttatgaac gtaacgaagt tgcaccaaac
     1741 aagccgtccg aagaagaata ccagcgccgt ctgcgtgaat cttataccgg tggcttcgtg
     1801 aaagaaccgg agaaaggcct gtgggagaac atcgtttacc tggactttcg tagcctgtac
     1861 ccatctatca tcatcactca caacgtttcc ccggataccc tgaacctgga aggttgtaag
     1921 aactacgaca tcgctccgca ggtaggtcac aaattttgca aagatatccc gggcttcatc
     1981 ccgagcctgc tgggccacct gctggaagag cgtcaaaaga tcaaaaccaa aatgaaagaa
     2041 actcaggatc cgatcgaaaa gatcctgctg gactaccgcc agaaagctat taaactgctg
     2101 gcaaacagct tttacggcta ctacggttat gcgaaggcac gctggtactg caaggaatgc
     2161 gcagaaagcg ttaccgcgtg gggccgcaaa tatatcgaac tggtatggaa agaactggaa
     2221 gaaaagttcg gtttcaaagt tctgtacatc gataccgacg gcctgtacgc taccatcccg
     2281 ggcggtgaat ctgaagagat caagaagaag gctctggagt tcgttaaata catcaactcc
     2341 aaactgccgg gtctgctgga gctggaatac gagggcttct ataaacgtgg cttcttcgta
     2401 accaagaaac gttatgcggt tatcgacgaa gaaggtaaag ttatcactcg tggtctggaa
     2461 atcgttcgcc gtgactggtc tgaaatcgct aaggagactc aggcgcgcgt tctggaaact
     2521 atcctgaagc acggtgacgt agaagaagct gtacgtatcg ttaaggaagt tatccagaaa
     2581 ctggcaaact acgaaatccc gccggaaaag ctggctatct acgaacagat cactcgcccg
     2641 ctgcatgaat acaaagcaat cggtccgcac gttgctgttg caaagaagct ggcggctaaa
     2701 ggtgtaaaga tcaaaccggg tatggttatc ggctacatcg tgctgcgcgg cgatggcccg
     2761 atcagcaacc gtgcgattct ggctgaagaa tacgacccga agaagcacaa atacgatgct
     2821 gaatactaca tcgagaacca ggttctgccg gcggttctgc gtattctgga aggcttcggc
     2881 taccgcaaag aagacctgcg ttaccaaaag actcgccagg ttggtctgac tagctggctg
     2941 aacatcaaga agtccggcac cggtggtggt ggcgcaactg tgaaatttaa atacaaaggt
     3001 gaagaaaagg aagttgacat ctctaaaatc aagaaagtgt ggcgcgttgg caaaatgatt
     3061 tctttcacct acgacgaagg tggcggtaaa accggccgtg gcgctgtttc tgagaaagac
     3121 gcaccgaagg agctgctgca gatgctggag aaacagaaga aataataagg atccatgggt
     3181 accctcgagt gaagacaagc ttaggtattt attcggcgca aagtgcgtcg ggtgatgctg
     3241 ccaacttagt cgagcaccac caccaccacc actgagtccg gctgctaaca aagcccgaaa
     3301 ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc
     3361 taaacgggtc ttgaggggtt ttttgctgaa aggaggaact ataccggatt ggcgaatggg
     3421 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     3481 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc ttctcgccac
     3541 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     3601 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtggcca
     3661 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     3721 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     3781 gggatttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     3841 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg
     3901 cggaacccct attgtttatt tttctaaata cattcaaata tgtatccgct catgaattaa
     3961 ttcttagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc
     4021 aataccatat ttttgaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc
     4081 cataggatgg caagatcctg gtatcggtct gcgattccga ctcgtccaac atcaatacaa
     4141 cctattaatt tcccctcgtc aaaaaaaggt tatcaagtga gaaatcacca tgagtgacga
     4201 ctgaatccgg tgagaatggc aaaagtttat gcatttcttt ccagacttgt tcaacaggcc
     4261 agccattacg ctcgtcatca aaatcactcg cacaaccaaa ccgttattca ttcgtgattg
     4321 cgcctgagcg agacgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga
     4381 atgcaaccgg cgcaggaaca ctgccagcgc atcaacaatt tttcacctga atcaggatat
     4441 tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca
     4501 tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattcgtc agccagttta
     4561 gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca
     4621 actctggcgc atcgggcttc ccatacaatc gatagattgt cgcacctgat tgccgacatt
     4681 atcgcgagcc catttatacc catataaatc agcatccatg ttggaattta atcgcggcct
     4741 agagcaagac gtttcccgtt gaatatggct cataacaccc cttgtattac tgtttatgta
     4801 gcagacagtt ttattgttca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     4861 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     4921 ctgctgctgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     4981 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     5041 cttctagtgt agcctagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     5101 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     5161 ggttggactc aagacgatag taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     5221 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     5281 gctatgagaa agcgccacgc ttcccgaagg agaaaggcgg acaggtatcc ggtaagcggc
     5341 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     5401 agtcctgtcg ggtttcgcca cctctgactt gagcgcgatt tttgtgatgc tcgtcagggg
     5461 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     5521 ggccttttgc tcacatgttc tttcctgcgt tatcccctga tttgtggata accgtattac
     5581 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     5641 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatt gtgcggtatt
     5701 tcacaccgca atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     5761 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaaaccc
     5821 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     5881 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag
     5941 ctgggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg
     6001 cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca
     6061 tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggattctgtt
     6121 catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga
     6181 tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg
     6241 gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt
     6301 ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct
     6361 gacttccggt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc
     6421 aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat
     6481 tctgctaacc agtaagcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat
     6541 catgcgcacc cgtggggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt
     6601 ggtggcggga ccagtgacga agcttgagcg agggcgtgca agattccgaa taccgcaagc
     6661 gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc
     6721 gctgccggca cctgtcctac gagttgcata taaagaagac agtcataagt gcggcgacga
     6781 tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg
     6841 gtcgagatcc cggtgcctaa tgagtgagct aacttaatta attgcgttgc gctcactgcc
     6901 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     6961 gagaggcggt ttgcgtattg ggcgccaggg tggtttttct tttaccagtg agacgggcaa
     7021 cagctgattg cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt
     7081 ttgccccagc aggcgaaaat cctgtttgat ggtggttaac ggcgggatat acatgagctg
     7141 tcttcggtat cgtcgtatcc cactaccgag atatccgcac caacgcgcag cccggactcg
     7201 gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat cgcagtggaa
     7261 cgatgccctc attcagcatt tgcatggttt gttgaaaacc ggacatggca ctccagtcgc
     7321 cttcccgttc cgctatcggc tgaatttgat tgcgagtgag atatttatgc cagccagcca
     7381 gacgagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc
     7441 caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact
     7501 gttgatgggt gctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct
     7561 tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt
     7621 tgcgcgagaa gattgtgccc gccgctttac aggcttcgac gccgcttcgt tctaccatcg
     7681 acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg
     7741 acggcgcgtg cagggccaga ctggagtggc aacgccaatc agcaacgact gtttgcccgc
     7801 cagttgttgt gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt
     7861 ttcccgcgtt ttcgcagaaa cgtggctggc ctgttcacca cgcgggaaac ggtctgataa
     7921 gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg
     7981 aattgactct cttccgggcg ctatcatgcc ataccgcgaa ggttttgcgc cattcgatgg
     8041 tgtccgggat ctcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt
     8101 aggttgaggc cgttgagcac cgccgccgca aggaatggtg catg
//
